pkIs296 [hsp::gsa-1(Q208L) + dpy-20(+)] X. Heat-shock promoter driving expression of constitutively active gsa-1 transgene. Suppressor of activated Gs. sgs-1 also called acy-1.

Heterozygotes are WT and segregate Dpys, Wt and arrested larvae that hardly move or pump. Suppressor of activated Gs. sgs-1 also called acy-1.

Flanking sequence: AGTCACTCTT - deletion - GAAGACTACT.

pk690 is a deletion allele within the gene C32E8.7. The deletion is stable in the homozygous state and has no obvious phenotype. Can verify the presence/homozygosity of the deletion by PCR using the following primers: AL1: 5'-CGACGACACTCCATTATTCC-3' AR1: 5'-CCAGTCCTGCAAAAATGCTC-3'. A product of about 3.7 kb is obtained from WT worms, while a product of about 1 kb is obtained from NL2003 worms. The deletion has been sequenced and covers position -354 to +2276.

Mut. RNAiR.

Resistant to feeding dsRNA.

Tc1 insertion in F16D3.2. Resistant to feeding dsRNA. Sensitive to gonadal injection of dsRNA.

Tc1 insertion in C34E11.1. Resistant to feeding dsRNA. Sensitive to gonadal injection of dsRNA.

Homozygous rrf-1 deletion allele. RNAi interference for genes expressed in somatic tissue is lost in rrf-1 deletion mutants.

Homozygous rrf-3 deletion allele. Increased sensitivity to RNAi when compared to WT animals. Deletion sequence (deletion in lower case letters, flanking undeleted sequence in capital letters): TGCACATATTctacagaatt ------- --------tacccgattaAATGGACAATT (from Plasterk Lab 11/05).

Osm. Odr; defective chemotaxis toward various odors. Reference: Lans H, Rademakers S, Jansen G. Genetics. 2004 Aug;167(4):1677-87.

pkIs1269 [gpa-13XS(+) + dpy-20(+)].

Heterozygotes are WT and segregate WT, blistered, and lethals. pk481 previously called spn-1.

TTTAAAACG TA CTTACCTTT

insertion: TTCTGTGAAC TA TGTCAAAAT

Dpyish.

Insertion in GGTCAAACG TA AGTTAAAGT.

pkIs1582 [let-858::GFP + rol-6(su1006)]. Rollers.

Mutator phenotype. Enhanced level of spontaneous mutations (frameshifts and single base pair substitutions). The genomic region that is deleted in NL2511 is from nt 24180-25956 (takes out exon 5 and part of exon 6).

ppw-1 animals are resistant to feeding of ds RNA directed against germline genes. Multiple polymorphisms in C18E3.7 including a single base deletion in ppw-1 resulting in an early stop codon.

Pick WT to maintain. The pk1331 allele can be followed by PCR.

pkIs1330 [hsp::gpa-12QL + dpy-20(+)]. Suppressor of activated G12.

Suppressor of activated Gs. Temperature sensitive allele.

pkIs296 [hsp::gsa-1(Q208L) + dpy-20(+)] X. Heat-shock promoter driving expression of constitutively active gsa-1 transgene. Suppressor of activated Gs. sgs-1 also called acy-1.

Resistant when fed dsRNA. Contains a point mutation at position 7120 (c to t) (W575 STOP).

Resistant to feeding dsRNA but sensitive to dsRNA injection.

Resistant when fed dsRNA. Contains a point mutation at position 13832 (c to t) (R1000 STOP).

Resistant to feeding dsRNA but sensitive to dsRNA injection. PKA rsd-8.

Reduced response to exogenously added dauer pheromone and thus defective in the regulation of dauer formation.

Reduced response to exogenously added dauer pheromone and thus defective in the regulation of dauer formation.

pkIs1604 [hsp-16.2::ATG(A)17GFP::LacZ + rol-6(su1006)]. Rollers.

pkIs1605 [hsp-16.2p::GFP::LacZ + rol-6(su1006)]. Rollers.

Heterozygotes are WT and segregate WT (heterozygotes), L1 arrested animals (pk44 homozygotes), and paralyzed Dpy Uncs (mnC1 homozygotes).

Reduced response to exogenously added dauer pheromone and thus defective in the regulation of dauer formation.

ppw-1 animals are resistant to feeding of ds RNA directed against germline genes. The genomic region that is deleted: nt 2479-3982 of C18E3 (intragenic deletion in C18E3.7). This strain was formerly called NL2557.

Mutator. Flanking sequence: cctatgatcatattattgacgactttagtc aatggcaaagaaagagtttttaaatgtcat. AKA mut-8.Mutator. Flanking sequence: cctatgatcatattattgacgactttagtc aatggcaaagaaagagtttttaaatgtcat.Mutator.

pkEx170 [gpb-1(+) + rol-6(su1006)]. Rollers. Pick Rollers to maintain. NL361 is homozygous for the gpb-1 deletion allele pk44; this results in an L1 arrest if the larvae has maternally derived GPB-1 or in an early embryonic lethality if there is no maternally derived GPB-1 for the developing embryo. This phenotype is rescued by the extrachromosomal transgene which contains the WT gpb-1 gene.

pkIs32[pie-1::GFP::H2B]. RNAi hypersensitive.

Twitcher Unc. Flanking sequence: agattgacgagatccataaggaaggatgta cattgaactggaagcctccaactgataacg.Twitcher Unc.

pkIs1600 [dpy-30::GFP(truncated) + rol-6(su1006)] I. ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Rollers.

pkIs1641[unc-119(+)]. Non-Unc strain.

pkIs1642[unc-119(+) + R11A8.5(+) + sir-2.1(+)]. Non-Unc strain.

Suppressor of activated Gs. Temperature sensitive allele.

Superficially wildtype.

pkIs1330 [hsp::gpa-12QL + dpy-20(+)]. Suppressor of activated G12.

pkIs2256 [alg-2::HA + rol-6(su1006)]. Rollers.

Right flanking sequence of Tc1: 5'-TATATTCCAGAAA.