DpyUnc.

Dpy. Slightly Unc. Semi-dominant suppressor. Suppressed Unc between 22.5 and 24C.

Dpyish/Suppressed. Variable expression.

Dpy. Coiler Unc. Closely linked. Doesn't grow at 25C.

Temperature sensitive dauer constitutive. Unc. Closely linked.

Temperature sensitive dauer constitutive. Long. Unc. Linked closely.

UncDpy.

Lon, slow-growing. Very poor growth at less than 20C.

tra-2 suppressed. Viable hermaphrodite. See also CGC 397. Grow between 22.5C and 24C.

tra-3 suppressed. Viable hermaphrodite. See also CGC 397.

Slightly Unc. Lannate resistant. Recessive. Larvae Unc. M-MATING++ 1-10%WT.

Lannate resistant. Recessive. M-MATING-NO SUCCESS.

Unc. Recessive. M-MATING-NO SUCCESS.

Heterozygotes are wild-type, and segregate wild-type heterozygotes, fertile wild-type males (tra-1 homozygotes), and Dpy. Can recombine: pick individual WT hermaphrodites and check for correct segregation of progeny to maintain.

Pick Unc hermaphrodite to maintain (these will be tra-1/+; unc-42 him-5 dpy-21 XO animals). Segregates Unc hermaphrodites (tra-1/+ III), Unc males (+/+ III), and DpyUnc hermaphrodites (+/+ III). dpy-21 not expressed in XO. dpy-21(e428) V; XX animals are weak Dpy. dpy-21(e428) V; XO animals are non-Dpy.

Balanced well. Animals with the duplication are WT. Animals which have lost the duplication have a hermaphrodite gonad and intestine (they are self-fertile); the rest of the body is male.

Balanced well. Animals with the duplication are WT. Animals which have lost the duplication have a hermaphrodite gonad and intestine (they are self-fertile); the rest of the body is male.

Slow moving Unc. Body muscle abnormal. Temperature sensitive. Recessive. M-MATING+POOR <1%WT.

Levamisole resistant. Recessive. Kinky Unc. M-MATING+POOR <1%WT.

Recessive Suppressor. XO Fertile hermaphrodite. WT phenotype.

Unc. Recessive. M-MATING++ 1-10%WT.

Bursae abnormal. Vulva protruding. Segregates abnormal males. M-MATING++ 1-10%WT.

Homozygous viable and looks almost normal by dissecting scope, but under Nomarski microscopy the gut has a very abnormal spongy appearance, which is especially noticeable in L4 larvae.

Semidominant Unc(mild). Intragenic revertants common.

Touch insensitive, lethargic.

Hermaphrodites are Dpy and segregate males. Males are non-Dpy and Mab. Both sexes show lineage alterations.

Abnormal large nucleoli in all cells.

Wild type strain segregating more wild type and embryonic lethals, in approximately 1:1 ratio.

Animals which have lost the Dup are thin, sterile, and uncoordinated. Maintain by picking WT (Egl).

Benomyl Resistant. Dominant at 25C--Recessive at 15C.

Males abnormal.

Variable failures in cytokinesis. Mostly viable and fertile.

WT hermaphrodites which segregate WT hermaphrodites and tra-1 homozygous XX; weakly masculinized class A7, never fertile as males.

Dpy. Mapping marker standard. Recessive. M-MATING++ 1-10%WT.

Stable male/female strain propagated by crossing tra-3(e1767)IV females x tra-3(e1767)IV males. tra-1(e1575) is semi-dominant and transforms both XX and XO into fertile females. Strain contains 4 genotypes: 1) e1575/+; e1767 XX which are fertile females. 2) e1575/+; e1767 XO which are fertile females. 3) e1767 XX which are infertile pseudomales. 4) e1767 XO which are fertile males.

Shrinker-contracts both dorsally and ventrally when prodded. Slow, poor backing. Slightly small. M-MATING++ 1-10%WT

Hermaphrodites and males superficially WT. Males lay down a gelatinous plug over the vulva of mated hermaphrodites. Plug formation controlled only by male genotype. Both plg-1 and plg-1/+ males make plugs.

Non-Dpy 4A;3X hermaphrodites segregating 4A;3X hermaphrodites, 4A;2X males and dead or very dumpy 4A;4X hermaphrodites. Reference: Strain 19 in Hodgkin (2002) PMID: 12399387

Males are missing most of the rays and additional alae extend into the tail.

Wildtype hermaphrodites segregating wild-type hermaphrodites and tra-1(q88) XX males, which are fertile and make yolk. Complex rearrangement of tra-1. Reference: Zarkower et al. (1994) PMID: 7520378

Heterozygotes are WT and segregate WT and DpyUnc. 1/3 of the WT are fem-2 homozygotes. Homozygous fem-2 animals are hermaphrodites if mother was heterozygous for fem-2, and are fertile females if mother was homozygous for fem-2.

Temperature sensitive. Sterile at 25C. Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. [2/98: Craig Mello noticed a different embryonic phenotype in this strain as compared to the e2141 stock that Jim Priess obtained from England-the ABp fate appears WT.] [NOTE (11/16/10 - J. Hubbard): This strain is NOT synonymous with glp-1(e2144) as previously reported in Kodoyianni V, Maine EM, Kimble J. (1992) [Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans. Mol Biol Cell. 3,1199-213. PMID: 1457827]. As reported in Worm Breeders Gazette December 2010; 18(3), e2144 carries the mutation c2785t in exon 8, leading to the amino acid change L929F, whereas e2141 carries the mutations c2920t and a3610g in exon 8, leading to the amino acid changes R974C and T1204A.]

Uncoordinated, shrinker.

Heterozygotes are WT and segregate WT, Uncs which are sterile and dead eggs (eDf2 homozygotes). Strain breaks down very rarely.

eDf43 pka lin-49(e2173). Heterozygotes are WT and segregate WT, Unc-36, and LinDpys. The lin-40 dpy-11 homozygotes are Dpy, Sterile and abnormal. Maintain by picking WT.

Wildtype hermaphrodites segregating wildtype hermaphrodites and tra-1 XX males, which mate with greater efficiency than most tra-1 XX mutants. Reference: Hodgkin (1987) PMID:3428597.

Spongy gut, intestine has foamy look, especially in L4; Temperature sensitive-almost normal at 15C; leaky Eat at 20C; Penetrant and sick Eat at 25C. Unc.

Slow growing. Homozygous gro-1(e2400) derived from PaC-1 (CB4507). Caenorhabditis elegans wild isolate CB subclone of PaC1 (Tc1 pattern IV?)].

Dpy hermaphrodites. Protruding vulva in adult; viable at all temperatures.

Grows poorly, best at 20C. Self-fertile intersexual XX hermaphrodite due to partial suppression of weak tra-1 allele by smg-2(NMD). References: Zarkower et al. (1994) PMID: 7520378. Hodgkin (1987) PMID:3428597.