maIs134 [lin-4p::GFP + unc-119(+)]. Wild type.

maIs135 [mir-237p::GFP + unc-119(+)]. Wild type.

maIs137 [let-7p::GFP + unc-119(+)]. Wild type.

maIs138 [mir-84p::GFP + unc-119(+)]. Wild type.

maIs140 [mir-241p::GFP + unc-119(+)]. Wild type.

maIs150 [mir-48p::GFP + unc-119(+)]. Wild type.

maIs162 [mir-59p::GFP + unc-119(+)]. Wild type.

maIs163 [mir-357-358p::GFP + unc-119(+)]. Wild type. [10/01/2018: Received new stock from VT following report from user that GFP expression pattern in original stock was not as expected.]

maIs173 [mir-242p::GFP + unc-119(+)]. Wild type.

maIs177 [mir-243p::GFP + unc-119(+)]. Wild type.

maIs180 [mir-244p::GFP + unc-119(+)]. Wild type.

maIs182 [mir-251p::GFP + unc-119(+)]. Wild type.

maIs184 [mir-51::GFP + unc-119(+)]. Wild type.

maIs185 [mir-2p::GFP + unc-119(+)]. Wild type.

maIs188 [mir-228p::GFP + unc-119(+)]. Wild type.

maIs189 [mir-54-56p::GFP + unc-119(+)]. Wild type.

maIs191 [mir-235p::GFP + unc-119(+)]. Wild type.

maIs196 [mir-227-80p::GFP + unc-119(+)]. Wild type.

maIs197 [mir-234p::GFP + unc-119(+)]. Wild type.

maIs206 [mir-81p::GFP + unc-119(+)]. Wild type.

maIs218 [mir-231p::GFP + unc-119(+)]. Wild type.

maIs220 [mir-360p::GFP + unc-119(+)]. Wild type.

maIs227 [mir-90p::GFP + unc-119(+)]. Wild type.

maIs229 [mir-85p::GFP + unc-119(+)]. Wild type.

maIs234 [mir-53::GFP + unc-119(+)]. Wild type.

maIs236 [mir-246p::GFP + unc-119(+)]. Wild type.

maIs251 [mir-1p::GFP + unc-119(+)]. Wild type.

maIs256 [mir-247-797p::GFP + unc-119(+)]. Wild type.

maIs261 [mir-265p::GFP + unc-119(+)] Wild type.

maIs267 [mir-266p::GFP + unc-119(+)]. Wild type.

maIs268 [mir-259p::GFP + unc-119(+)]. Wild type.

maIs276 [mir-60p::GFP + unc-119(+)]. Wild type.

maIs278 [mir-788p::GFP + unc-119(+)]. Wild type.

maIs300 [mir-82p::GFP + unc-119(+)]. Wild type.

maIs347 [mir-793p::GFP + unc-119(+)]. Wild type.

maIs348 [mir-794p::GFP + unc-119(+)]. Wild type.

maIs352 [mir-71p::GFP + unc-119(+)]. Wild type.

Heterozygotes are superficially WT (with DTC migration defects), and segregate superficially WT (with DTC migration defects), sterile ts-Dpy qC1 homozygotes, and st564 homozygotes (PAT lethal). Pick WT and check for correct segregation of progeny to maintain. Reference: Burke SL, Hammell M, Ambros V. Genetics. 2015 Jun 15.

Pick wild-type GFP+ to maintain. Heterozygotes are WT with GFP+ pharynx, and segregate Dpy GFP+ mIn1 homozygotes, and GFP- low viability non-Egl hermaphrodites. Viability of first generation nDf50 homozygotes (GFP-) segregated from balanced heterozygotes is rescued by maternal contribution of mir-35-41 from balancer. Second generation GFP- animals are low viability. Reference: McJunkin K, Ambros V. Genes Dev. 2017 Feb 15;31(4):422-437.

maEx246 (cdc-42p::GFP::H2B::cdc-42(mutated) 3`UTR + cdc-42p::mCherry::H2B::cdc-42 3`UTR + pBluescript + pIF9 unc-119(+) + pCFJ150 + pCFJ210). Pick non-Unc to maintain. Reference: Burke SL, Hammell M, Ambros V. Genetics. 2015 Jun 15.

Endogenous sup-26 locus tagged with 3xFLAG. Reference: McJunkin K & Ambros V. Genes Dev. 2017 Feb 15;31(4):422-437. PMID: 28279983

maEx246 (cdc-42p::GFP::H2B::cdc-42(mutated) 3`UTR + cdc-42p::mCherry::H2B::cdc-42 3`UTR + pBluescript + pIF9 unc-119(+) + pCFJ150 + pCFJ210). Pick non-Unc to maintain. Reference: Burke SL, Hammell M, Ambros V. Genetics. 2015 Jun 15.

maEx247 (pat-3p::GFP::H2B::pat-3(mutated) 3`UTR + pat-3p::mCherry::H2B::pat-3 3`UTR + pBluescript + pIF9 unc-119(+) + pCFJ150 + pCFJ210). Pick non-Unc to maintain. DTC migration defects. Reference: Burke SL, Hammell M, Ambros V. Genetics. 2015 Jun 15.

maEx247 (pat-3p::GFP::H2B::pat-3(mutated) 3`UTR + pat-3p::mCherry::H2B::pat-3 3`UTR + pBluescript + pIF9 unc-119(+) + pCFJ150 + pCFJ210). Pick non-Unc to maintain. Reference: Burke SL, Hammell M, Ambros V. Genetics. 2015 Jun 15.

Heterozygotes are Dpy and segregate Dpy, males and dead eggs.

Pick wild-type GFP+ to maintain. Heterozygotes are WT with GFP+ pharynx, and segregate Dpy GFP+ mIn1 homozygotes, and GFP- low viability non-Egl hermaphrodites. Viability of first generation nDf50 homozygotes (GFP-) segregated from balanced heterozygotes is rescued by maternal contribution of mir-35-41 from balancer. Second generation GFP- animals are low viability. Reference: McJunkin K, Ambros V. Genes Dev. 2017 Feb 15;31(4):422-437.

Endogenous nhl-2 locus tagged at the N-terminus with GFP and 3xFLAG. Reference: McJunkin K & Ambros V. Genes Dev. 2017 Feb 15;31(4):422-437. PMID: 28279983

Only gives a decent brood size at 20C. At 15C: Muv/Blip. At 20C: some Muv/Blip, some Blip but not Muv. At 25C: Not Muv, but do have Blip (due to lin-12). Egl- at all temps.

maIs103 [rnr::GFP + unc-36(+)].