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Strain Species Genotype
CA1199 C. elegans unc-119(ed3) III; ieSi38 IV. Show Description
ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635.
CA1200 C. elegans ieSi57 II; unc-119(ed3) III. Show Description
ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. This strain can be used for auxin-inducible degradation (AID) in somatic tissues. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635.
CA1204 C. elegans unc-119(ed3) III; ieSi58 IV. Show Description
ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (oxTi177) expressing degron::GFP in the soma. This strain can be combined with different TIR1 strains to test auxin-inducible degradation (AID) of protein in somatic tissue. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635.
CA1205 C. elegans unc-119(ed3) III; ieSi59 III. Show Description
ieSi59 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] III. Single copy transgene inserted into chromosome III (oxTi444) expressing degron::GFP at low levels in the soma. This strain can be combined with different TIR1 strains to test auxin-inducible degradation (AID) of protein in somatic tissue. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635.
CA1206 C. elegans ieSi57 II; ieSi59 III. Show Description
ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. ieSi59 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] III. ieSi57 is a single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. ieSi59 is a single copy transgene inserted into chromosome III (oxTi444) expressing degron::GFP at low levels in the soma. This strain can be used as control for auxin-inducible degradation (AID) in somatic tissues. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635.
CA1208 C. elegans ieSi60 II; unc-119(ed3) III. Show Description
ieSi60 [myo-2p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in pharyngeal muscle. This strain can be used for auxin-inducible degradation (AID) in pharyngeal muscle. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635.
CA1209 C. elegans ieSi61 II; unc-119(ed3) III. Show Description
ieSi61 [ges-1p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the intestine. This strain can be used for auxin-inducible degradation (AID) in the intestine. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635.
CA1218 C. elegans syp-3(ok758) I; ieSi11 II; unc-119(ed3) III. Show Description
ieSi11 [syp-3p::EmeraldGFP::syp-3::syp-3 3'UTR + Cbr-unc-119(+)] II. ieSi11 was inserted into ttTi5605 II using MosSCI. Expression of GFP::SYP-3 largely complements syp-3(ok758), but some meiotic nondisjunction is detected above the N2 background (85% embryonic viability; ~1% male self-progeny;). GFP::SYP-3 expression is readily detected in spermatocytes and oocytes in the germline, and localizes to the interface between paired homologous chromosomes during most of meiotic prophase. Reference: Rog O, Dernburg AF. Cell Rep. 2015 Mar 10. pii: S2211-1247(15)00178-3.
CA1219 C. elegans unc-119(ed3) III; ieSi21 IV. Show Description
ieSi21 [sun-1p::sun-1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ieSi21 was inserted into cxTi10882 IV using MosSCI. Expression of the transgenic SUN-1::mRuby fusion protein complements the sun-1 deletion allele. SUN-1::mRuby is expressed throughout the germline and in the early embryo, where it localizes to nuclear envelope and associates with chromosome pairing centers during early meiotic prophase. Reference: Rog O, Dernburg AF. Cell Rep. 2015 Mar 10. pii: S2211-1247(15)00178-3.
CA1230 C. elegans htp-3(tm3655) I; ieSi6 II; unc-119(ed3) III. Show Description
ieSi6 [htp-3p::htp-3::GFP + Cbr-unc-119(+)] II. Maintain at 20C. Although silencing of the transgene has not observed, it may be helpful to maintain it over htp-3(tm3655) to continually select for expression. unc-119(ed3) might not be homozygous in this strain. Reference: Kim et al. Dev Cell. 2015 Oct 26;35(2):247-61.
CA1352 C. elegans ieSi64 II; unc-119(ed3) III. Show Description
ieSi64 [gld-1p::TIR1::mRuby::gld-1 3’UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing a modified Arabidopsis thaliana TIR1 tagged with mRuby in the germ line and early embryos. Comparing to CA1472, this strain expresses a higher level of TIR1 and can induce a faster degradation of AID-tagged proteins in the germ line and early embryos. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635.
CA1353 C. elegans ieSi65 II; unc-119(ed3) III. Show Description
ieSi65 [sun-1p::TIR1::sun-1 3’UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing a modified Arabidopsis thaliana TIR1 in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635.
CA1472 C. elegans ieSi68 II; unc-119(ed3) III. Show Description
ieSi68 [sun-1p::TIR1::mRuby::htp-1 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing a modified Arabidopsis thaliana TIR1 tagged with mRuby in the germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635.
CB1 C. elegans dpy-1(e1) III. Show Description
Dpy.
CB1068 C. elegans unc-79(e1068) III. Show Description
Unc-fainter. Stops. Stiff anterior.
CB1071 C. elegans mor-1(e1071) III. Show Description
Head abnormal. Movement almost wild-type.
CB109 C. elegans unc-16(e109) III. Show Description
Unc-sluggish. Small.
CB1122 C. elegans cccp-1(e1122) III. Show Description
Movement slow. Egg retainer. Recessive. M-MATING+++ 10-30%WT.
CB1259 C. elegans dpy-19(e1259) III. Show Description
Temperature sensitive Dpy. Reproduction poor at 25C. M-MATING+ AT LOW TEMPERATURE ONLY.
CB1291 C. elegans unc-79(e1291) III. Show Description
Unc-fainter: stops.
CB1295 C. elegans dpy-17(e1295) III. Show Description
Medium Dpy.
CB1314 C. elegans dpy-19(e1314) III. Show Description
Temperature sensitive Dpy.
CB1322 C. elegans lin-8(n111) II; lin-9(n112) III. Show Description
Multivulva.
CB1345 C. elegans dpy-17(e1345) III. Show Description
Dpy. Temperature sensitive.
CB1364 C. elegans daf-4(e1364) III. Show Description
Temperature sensitive dauer constitutive. 100% dauers at 25C. Dauer recovery poor at 15C. Small. M-Mating--no success.
CB1370 C. elegans daf-2(e1370) III. Show Description
Temperature sensitive dauer constitutive. Maintain at 15C. 100% dauers at 25C. 15% dauer formation at 20C. Long-lived. M-MATING+LOW TEMPERATURE ONLY.
CB1372 C. elegans daf-7(e1372) III. Show Description
Temperature sensitive dauer constitutive. Crowds. 100% dauers at 25C. Leaky at 20C. Growth slow. M-MATING+LOW TEMPERATURE ONLY.
CB1416 C. elegans unc-86(e1416) III. Show Description
Egg-laying deficient. Lineage abnormal. Mechanosensory abnormal. M-MATING++ 1-10%WT. Serotonin def.
CB1464 C. elegans sup-5(e1464) III. Show Description
Suppressor. Null allele. Sterile at 15C. Grow between 22.5 and 24C.
CB1507 C. elegans unc-86(e1507) III. Show Description
HSN-. Egl. Mec.
CB1511 C. elegans him-10(e1511) III. Show Description
Temperature sensitive. 2% XO self-progeny at 15C, 12% at 20C, and 27% at 25C. M-MATING++++ >30%WT.
CB1517 C. elegans eDf2 III; eDp6 (III;f). Show Description
DpyUnc. See also WBPaper0000498 and WBPaper00002306.
CB156 C. elegans unc-25(e156) III. Show Description
Shrinker-contracts both dorsally and ventrally when prodded. Slow, poor backing. Slightly small.
CB1562 C. elegans vab-7(e1562) III. Show Description
Unc. Tail abnormalities-twisted or knobbed. Recessive. M-MATING-NO SUCCESS.
CB1597 C. elegans unc-103(e1597) III. Show Description
Semidominant. Paralyzed Unc. Prone to spontaneous suppression.
CB164 C. elegans dpy-17(e164) III. Show Description
Dpy. Larvae abnormal. M-MATING+++ 10-30%WT.
CB185 C. elegans lon-1(e185) III. Show Description
Long. Abnormal bursae. Epistatic to Small. Recessive. M-MATING-NO SUCCESS.
CB189 C. elegans unc-32(e189) III. Show Description
Coiler Unc. Severe. Recessive. M-MATING-NO SUCCESS.
CB1918 C. elegans lon-1(e185) unc-32(e189) III. Show Description
Long. Unc. Linked closely.
CB2053 C. elegans dpy-18(e364) unc-64(e246) III. Show Description
DpyUnc.
CB2095 C. elegans dpy-1(e1) sup-5(e1877) III; unc-51(e369) V. Show Description
Dpy. Slightly Unc. Semi-dominant suppressor. Suppressed Unc between 22.5 and 24C.
CB2108 C. elegans dpy-18(e364) sup-1(e995) III. Show Description
Dpyish/Suppressed. Variable expression.
CB2195 C. elegans dpy-19(e1259) unc-32(e189) III. Show Description
Dpy. Coiler Unc. Closely linked. Doesn't grow at 25C.
CB2196 C. elegans daf-4(e1364) unc-32(e189) III. Show Description
Temperature sensitive dauer constitutive. Unc. Closely linked.
CB2206 C. elegans lon-1(e185) daf-4(e1364) unc-32(e189) III. Show Description
Temperature sensitive dauer constitutive. Long. Unc. Linked closely.
CB2233 C. elegans unc-69(e587) dpy-18(e364) III. Show Description
UncDpy.
CB2305 C. elegans lon-1(e185) sup-5(e1464) III. Show Description
Lon, slow-growing. Very poor growth at less than 20C.
CB2317 C. elegans tra-2(e1425) II; sup-5(e1464) III. Show Description
tra-2 suppressed. Viable hermaphrodite. See also CGC 397. Grow between 22.5C and 24C.
CB2318 C. elegans sup-5(e1464) III; tra-3(e1107) IV. Show Description
tra-3 suppressed. Viable hermaphrodite. See also CGC 397.
CB239 C. elegans ric-1(e239) III. Show Description
Slightly Unc. Lannate resistant. Recessive. Larvae Unc. M-MATING++ 1-10%WT.