| DCR9211 |
C. elegans |
unc-17(ola503[unc-17::GFP FLP-on]) IV. Show Description
GFP FLP-on inserted at C-terminus of endogenous unc-17 locus. Part of SynaptoTagMe toolkit for examining cell-specific cholinergic transmission. See https://elifesciences.org/reviewed-preprints/108675 for sequencing information. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
|
| DCR9265 |
C. elegans |
unc-17(syb7251[GFP::SL2::unc-17 FLP-on]) IV. Show Description
GFP::SL2 FLP-on inserted at N-terminus of endogenous unc-17 locus. Part of SynaptoTagMe toolkit for examining cell-specific cholinergic transmission. See https://elifesciences.org/reviewed-preprints/108675 for sequencing information. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
|
| DCR9374 |
C. elegans |
unc-17(syb7882[unc-17::mRuby3 FLP-on]) IV. Show Description
mRuby3 FLP-on inserted at C-terminus of endogenous unc-17 locus. Part of SynaptoTagMe toolkit for examining cell-specific cholinergic transmission. See https://elifesciences.org/reviewed-preprints/108675 for sequencing information. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
|
| DCR9575 |
C. elegans |
eat-4(syb8568[eat-4::GFP FLP-on]) III. Show Description
GFP FLP-on inserted at C-terminus of endogenous eat-4 locus. Part of SynaptoTagMe toolkit for examining cell-specific glutamatergic transmission. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
|
| DCR9681 |
C. elegans |
eat-4(syb9139[eat-4::mRuby3 FLP-on]) III. Show Description
mRuby3 FLP-on inserted at C-terminus of endogenous eat-4 locus. Part of SynaptoTagMe toolkit for examining cell-specific glutamatergic transmission. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
|
| DQM1113 |
C. elegans |
bmdSi297 II. Show Description
bmdSi297 [loxN::rpl-28p::FRT3::STOP::FRT3::TIR1(F79G)::T2A::DHB::2xmKate2]; inserted into safe harbor site ttTi5605 in Chr II. Ubiquitous rpl-28 promoter driving expression of FRT3::STOP::FRT3::TIR1(F79G)::DHB construct dependent upon tissue-specific FLPase. High levels of TIR1(F79G) can be expressed in specific tissue or cell types via FLPase activity, allowing spatiotemporally-targeted degradation of AID-tagged proteins. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 |
|
| DQM1244 |
C.elegans |
bmdSi327 I. Show Description
bmdSi327 [loxN::ckb-3p::FLP::P2A::H2B::2xmTurq2]; inserted into safe harbor site ttTi4348 in Chr I. Uterine-specific expression of FLPase in Z1/Z4 and their descendants with blue fluorescent histone reporter for visualization. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 |
|
| DQM1256 |
C. elegans |
bmdSi346 I; bmdSi297 II. Show Description
bmdSi346 [loxN::lin-31p::FLP]; inserted into safe harbor site ttTi4348 in Chr I. bmdSi297 [loxN::rpl-28p::FRT3::STOP::FRT3::TIR1(F79G)::T2A::DHB::2xmKate2]; inserted into safe harbor site ttTi5605 in Chr II. FLP-ON::TIR1 system for AID-tagged protein degradation in VPCs. High levels of TIR1(F79G) expression in vulval precursor cells by lin-31p::FLP with co-expression of CDK activity sensor. bmdSi297 contains the ubiquitous rpl-28 promoter driving expression of FRT3::STOP::FRT3::TIR1(F79G)::DHB construct dependent upon tissue-specific FLPase. High levels of TIR1(F79G) can be expressed in specific tissue or cell types via FLPase activity, allowing spatiotemporally-targeted degradation of AID-tagged proteins. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 |
|
| DQM1258 |
C. elegans |
bmdSi348 I. Show Description
bmdSi348 [loxN::rgef-1p::FLP::P2A::H2B::2xmTurq2]; inserted into safe harbor site ttTi4348 in Chr I. Pan-neuronal expression of FLPase with blue fluorescent histone reporter for visualization. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 |
|
| DQM1260 |
C. elegans |
bmdSi350 I. Show Description
bmdSi350 [loxN::wrt-2p::FLP::P2A::H2B::2xmTurq2]; inserted into safe harbor site ttTi4348 in Chr I. Hypodermal (seam cell) expression of FLPase with blue fluorescent histone reporter for visualization. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 |
|
| DQM1283 |
C. elegans |
bmdSi348 I; bmdSi362 II. Show Description
bmdSi348 [loxN::rgef-1p::FLP::P2A::H2B::2xmTurq2]; inserted into safe harbor site ttTi4348 in Chr I. bmdSi362 [loxN::rpl-28p::FRT3-LCK::mNG-STOP::FRT3::TIR1(F79G)::2A::PH::2xmKate2]; inserted into safe harbor site ttTi5605 in Chr II. FLP-ON::TIR1 system for AID-tagged protein degradation in neurons. High levels of TIR1(F79G) expression in neurons by rgef-1p::FLP with co-expression of membrane markers. bmdSi362 contains the ubiquitous rpl-28 promoter driving expression of FRT3-LCK::mNG-STOP::FRT3::TIR1(F79G)::2A::PH::2xmKate2 construct dependent upon tissue-specific FLPase. High levels of TIR1(F79G) can be expressed in specific tissue or cell types via FLPase activity, allowing spatiotemporally-targeted degradation of AID-tagged proteins. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 |
|
