| F53B6.4(sta15) I. Show Description
sta15 is a CRISPR-engineered 31bp deletion in exon 2 of F53B6.4 that causes in early stop codons and a predicted full knockout. Made with a co-conversion of dpy-10 in parental N2 strain; presumably chased away during out-crossing. Left flanking Sequence of the deletion: AATCATCAAAGTCTTCGAAATCGAAGAAAG ; Right flanking sequence of the deletion: TTCAACTGTTTCCGCTGCTACTGGTGTTTC . sgRNA #1: GTCAAGACTGCTACCACCTC. Reference: Miklas JK, et al. Cell Rep. 2026 Aug 27;45(9):117902. doi: 10.1016/j.celrep.2026.117902. PMID: 42658673. |