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Strain Species Genotype
BC15557 C. elegans dpy-5(e907) I; sEx15557. Show Description
sEx15557 [rCes F47C10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
VC1296 C. elegans F47C10(gk584) V. Show Description
F47C10. Superficially wild type. External left primer: TCCGATACTTCATGCCAACA. External right primer: ATGGTGGTTTTGTAGCAGGC. Internal left primer: AATCTGCATGATCGGCATTT. Internal right primer: ATACCTACGTGCCTGCCAAC. Internal WT amplicon: 2445 bp. Deletion size: 638 bp. Deletion left flank: GGTAGAGTACCGAAAGCTGAAACTTTGATT. Deletion right flank: ATTTGAAAACATTTATATCAACTTTTAGAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1471 C. elegans nhr-186(gk732) V. Show Description
F47C10.3. External left primer: GAGGTCAATACAACGCCGAT. External right primer: TGTCCATTCGCAATTTTTGA. Internal left primer: ATATTTGCGACCGGCATTTA. Internal right primer: AAGCCTTGCCTATTTCCGAT. Internal WT amplicon: 2226 bp. Deletion size: 2034 bp. Deletion left flank: CGATATCGGCATGCTCGGCATCGCGTGGAG. Deletion right flank: TGAAAAAAAAAACAGAGAACTGTAAATTTT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2007 C. elegans nhr-185(gk1066) V. Show Description
This strain is homozygous for a deletion (gk1066) in F47C10.1, detectable by PCR using the following primers. External left primer: TCATTCTGGCAGGAAATTCA. External right primer: GGCGTAACGAAGTCCGATAA. Internal left primer: TCCGGTTAGTCCTGCAATTC. Internal right primer: CTGCTACCCATGTCGAGTGA. Internal WT amplicon: 2231 bp. Deletion size: 1770 bp. Deletion left flank: TTGCAAATTGAACATTGTGTGAAGCTGAGC. Deletion right flank: ATTGAATAGAACAATTTGGTACTAATTGAA. Validation: gk1066 passed by diagnostic PCR and CGH. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2051 C. elegans nhr-185(gk957) V. Show Description
F47C10.1. External left primer: TCATTCTGGCAGGAAATTCA. External right primer: GGCGTAACGAAGTCCGATAA. Internal left primer: TCCGGTTAGTCCTGCAATTC. Internal right primer: CTGCTACCCATGTCGAGTGA. Internal WT amplicon: 2231 bp. Deletion size: 847 bp. Deletion left flank: AGCTGAGCCCGGTAGATGTCGGACCACTAA. Deletion right flank: GAGGTATAAATAAAAGTCTATAAGAAAGAC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807