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Strain Species Genotype
RB1654 C. elegans F31E3.2(ok2044) III. Show Description
F31E3.2. Homozygous. Outer Left Sequence: ATTCGAAAGTCCACCGTCTG. Outer Right Sequence: CATTTCTTCCGCAACTCACA. Inner Left Sequence: GATGGACCAGCGAAGAAAAG. Inner Right Sequence: GGGCGTGAAGAACAGTGAAT. Inner Primer PCR Length: 2860 bp. Deletion Size: 1289 bp. Deletion left flank: AAATACATTTGTGACGTCACAAATGTATTT. Deletion right flank: TGTGGTAGGAACTATTTTCATTCACTTATT. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC447 C. elegans ceh-20(ok541) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
F31E3.2d. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok541 homozygotes (arrested DpyUnc). Homozygous hT2[bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT GFP and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807