wgIs710 [F21A10.2::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org).

zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10655 [F21A10.2::H1-wCherry::let-858 3' UTR]. May still contain stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)] in the background.

F21A10.2. External left primer: ACCCGAACAATAACACGCTC. External right primer: GGCCATTTCTCCGTCATTTA. Internal left primer: CATCAGTGGCAGTTGCGTAT. Internal right primer: CGCGAGAAAGAAAGAATTGC. Internal WT amplicon: 2333 bp. Deletion size: 996 bp. Deletion left flank: CATAAAAATGTTTTCAAGCACTTCAGATAT. Deletion right flank: CACGCGGGCACCCTTGCCTGACTGGGGGGT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807