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Strain Species Genotype
CB4856 C. elegans Show Description
Isolated from a pineapple field in Hawaii in 1972 by L. Hollen. Wild type. Low copy Tc1; pattern IX. C. elegans wild isolate. CB subclone of HA-8 (Tc1 pattern IX). See also WBPaper00005369. [NOTE (4-2014): Users reported abnormalities in CB4856 in late 2013 and early 2014. The current working stock at the CGC was thawed in 2-2014 from stock frozen in 1995.]
CX11400 C. elegans kyIR9 (X: ~4745910 - ~4927296, N2>CB4856) X. Show Description
kyIR9 [X: ~4745910 - ~4927296, N2>CB4856] X. LG X contains N2 from ~4745910 - ~4927296; the rest is from CB4856. QX9 was crossed to CB4856; a recombinant F2 with N2 npr-1 and CB4856 tyra-3 was isolated. Its progeny was back-crossed to CB4856 8 times, picking npr-1 hets each round prior to homozygosing.
GN231 C. elegans pgIR6 (X, CB4856>N2) X. Show Description
pgIR6 (X, CB4856>N2). CB4856 Chromosome X in N2 background.
GN67 C. elegans pgIR2 (II, CB4856>N2) II. Show Description
pgIR2 (II, CB4856>N2). CB4856 Chromosome II in N2 background.
GN68 C. elegans pgIR3 (III, CB4856>N2) III. Show Description
pgIR3 (III, CB4856>N2). CB4856 Chromosome III in N2 background.
GN69 C. elegans pgIR4 (IV, CB4856>N2) IV. Show Description
pgIR4 (IV, CB4856>N2). CB4856 Chromosome IV in N2 background.
GN70 C. elegans pgIR5 (V, CB4856>N2) V. Show Description
pgIR5 (V, CB4856>N2). CB4856 Chromosome V in N2 background.
MJF1 C. elegans chpIR1 (M, CB4856 > N2). Show Description
Reduced lifespan and reduced mitochondrial membrane potential. Transmitochondrial cybrid worm strain was bred to be homoplasmic for the CB4856 mtDNA genome in the N2 nuclear background. Reference: Dingley SD, et al. J Mol Biol. 2014 May 29;426(11):2199-216.
PX631 C. elegans fxSi3 I; fxSi4 II; fog-2(q71) V. Show Description
fxSi3 [hsp-16.41p::PEEL-1::tbb-2 3' UTR + rpl-28p::mKate2::unc-54 3'UTR + rps-0p::HygR::unc-54 3' UTR, I:2851003] I. fxSi4 [hsp-16.41p::PEEL-1::tbb-2 3'UTR + loxP, II: 8420157] II. Heat-shock strain can be maintained at 20C without any issues. Degron tag was inserted into the endogenous spe-44 locus, allowing auxin-inducible spermatogenesis arrest and reversible male sterility. Heat-shock-induced expression of PEEL-1 will cause lethality in both sexes. Five generations of lab adaptation following genome editing, all in the CB4856 background. Reference: Kasimatis, KR et al. (2021) Post-Insemination Selection Dominates Pre-Insemination Selection in Driving Male Competitive Ability. bioRxiv doi: https://doi.org/10.1101/2021.06.23.449605
QG1 C. elegans qgIR1 (X, CB4856>N2) X. Show Description
qgIR1 (X, CB4856>N2, haw102573 to 4,822,488) X. The strain is a nearly isogenic line that carries the CB4856 version of npr-1 in an otherwise N2 genetic background. NIL derived from RIAIL QX58 linked then unlinked to mec-2 from CB3273 lon-2 mec-2, then backcrossed to lon-2 for 12 generations selecting nonLon worms, then homozygosed. Genotyped N2 at pkP6106 and pkP6145. Based on QG613 sequencing, interval is from X:4,754,307-4,864,273.
WE5236 C. elegans pgIR1 (I, CB4856>N2) I. Show Description
pgIR1 (I, CB4856>N2). CB4856 Chromosome I in N2 background.
WM179 C. elegans nmy-2(ne3409) I. Show Description
Isolated from Hawaiian strain CB4856. Temperature sensitive embryonic lethal. Cytokinesis failure and polarity defects at 25C. Maintain at 15C. RNAi sensitive.
WM180 C. elegans nmy-2(ne1490) I. Show Description
Isolated from Hawaiian strain CB4856. Temperature sensitive embryonic lethal. Cytokinesis failure and polarity defects at 25C. Maintain at 15C. RNAi sensitive.