WM230 |
C. elegans |
drh-3(ne3197) I. Show Description
Mutator. Dominant RNAi-deficient. Temperature-sensitive sterile. Maintain at 15-20C. ne3197 is a G840D missense mutation. Reference: Gu W, et al. Mol Cell. 2009 Oct 23;36(2):231-44.
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WM231 |
C. elegans |
drh-3(ne4254) I. Show Description
Mutator. Dominant RNAi-deficient. Temperature-sensitive sterile. Maintain at 15-20C. ne4254 is a G802E missense mutation. Reference: Gu W, et al. Mol Cell. 2009 Oct 23;36(2):231-44.
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WM242 |
C. elegans |
neSi12 II; unc-119(ed3) III. Show Description
neSi12 [cdk-1::GFP + Cbr-unc-119(+)] II. Reference: Shirayama M, et al. Cell. 2012 Jul 6;150(1):65-77.
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WM27 |
C. elegans |
rde-1(ne219) V. Show Description
RNAi deficient.
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WM274 |
C. elegans |
prg-1(tm872) I; neSi14 II; unc-119(ed3) III. Show Description
neSi14 [FLAG::prg-1 + Cbr-unc-119(+)] II. MosSCI insertion into ttTi5605 (LG II). Reference: Lee HC, et al. Cell. 2012 Jul 6;150(1):78-87.
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WM275 |
C. elegans |
prg-1(tm872) I; neSi15 II; unc-119(ed3) III. Show Description
neSi15 [FLAG::prg-1(D583A) + Cbr-unc-119(+)] II. MosSCI insertion into ttTi5605 (LG II). Reference: Lee HC, et al. Cell. 2012 Jul 6;150(1):78-87.
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WM28 |
C. elegans |
unc-32(e189) III; rde-1(ne219) V. Show Description
RNAi deficient. Unc.
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WM29 |
C. elegans |
rde-2(ne221) I. Show Description
RNAi deficient. Transposon high-hopper strain. Him. Weak sterility.
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WM30 |
C. elegans |
mut-2(ne298) I. Show Description
RNAi deficient. Transposon high-hopper strain. Him. 10% dead embryos probably due to nondisjunction.
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WM43 |
C. elegans |
gex-3(zu196) IV/nT1 [unc-?(n754) let-?] (IV;V). Show Description
Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Zygotic phenotype: 100% of gex-3 homozygotes become Egl although they all make a normal looking L3/L4 vulva. Embryonic phenotype: complete loss of morphogenesis - hypodermal cells fail to intercalate or migrate. Received new stock from Erik Lundquist 11/2003.
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WM45 |
C. elegans |
rde-1(ne300) V. Show Description
RNAi deficient. Reference: Tabara H, et al. Cell 1999 Oct 15:99(2):123-32.
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WM49 |
C. elegans |
rde-4(ne301) III. Show Description
RNAi deficient.
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WM53 |
C. elegans |
alg-2(ok304) II. Show Description
T07D3.7. Homozygous viable, contains an out of frame deletion removing nucleotides encoding amino acids 34-374. This strain cannot be distributed to for-profit companies. Do not distribute this strain; other labs should request it from the CGC. URL: http://www.celeganskoconsortium.omrf.org.
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WM73 |
C. elegans |
mbk-2(ne992) IV. Show Description
Defective in mitotic spindle orientation. Mis-segregation of germline proteins PGL-1 and PIE-1. Change of cell fate (extra-gut, no gut). Defective in chromosome segregation. Temperature sensitive; maintain at 15C; 100% lethal at 25C.
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WM75 |
C. elegans |
wrm-1(tm514) III; neIs2 IV. Show Description
neIs2 [wrm-1::GFP + rol-6(su1006)] IV. Rollers.
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WM79 |
C. elegans |
rol-6(n1270) II; neEx1. Show Description
Rollers. n1270 is phenotypically wild type. neEx1[LIT-1::GFP + rol-6(su1006)]. LIT-1::GFP has full length LIT-1 fused to GFP in a YAC. Pick Rollers to maintain.
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WM93 |
C. elegans |
lit-1(ne1991) III. Show Description
Temperature sensitive embryonic lethal. Maintain at 15C.
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WM98 |
C. elegans |
sma-2(e502) ced-7(n1892) cdk-1(ne236)/qC1 [dpy-19(e1259) glp-1(q339) III. Show Description
Heterozygotes are WT and segregate WT, Smalls which produces dead eggs, and Dpy Steriles. Throws males.
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WM99 |
C. elegans |
cdk-1(ne2257) III. Show Description
Maintain at 15C. Produces dead eggs at 25C.
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WN2002 |
C. elegans |
Show Description
C. elegans wild isolate. Reference: Andersen EC, et al. Nat Genet. 2012 Jan 29;44(3):285-90.
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WOP122 |
C.elegans |
ahcy-1(syb646[ahcy-1::GFP]) I. Show Description
GFP tag inserted at C-terminus of endogenous ahcy-1 locus. Derived by out-crossing parental strain PHX646 two times to N2. Reference: Thapa P, et al. NPJ Aging. 2023 Dec 5;9(1):27. doi: 10.1038/s41514-023-00125-1. PMID: 38052822.
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WOP159 |
C.elegans |
ahcy-1(syb784 *syb646[ahcy-1(Y145C)::GFP]) I. Show Description
Engineered Y145C substitution mutation in endogenously GFP-tagged ahcy-1 locus. ahcy-1(Y145C) mutation mimics the pathogenic human mutation AHCY Y143C. ahcy-1(Y145C) mutants have a prolonged lifespan and are larger than control animals. ahcy-1(Y145C) mutants are fertile and produce a brood of laid and hatched eggs similar to control animals. ahcy-1(Y145C) mutants show a slight increase in SAH and a decrease in SAM levels, leading to an increased SAH to SAM ratio. See WOP122 for control strain. Derived by out-crossing parental strain PHX784 two times to N2. Reference: Thapa P, et al. NPJ Aging. 2023 Dec 5;9(1):27. doi: 10.1038/s41514-023-00125-1. PMID: 38052822.
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WRM45 |
C. elegans |
mex-3(spr5[*tn1753]) I. Show Description
Homozygous fertile; reduced brood size. mex-3(spr5) is a CRISPR/Cas9 engineered deletion removing 488 bp of the mex-3 3´UTR. Derived by modification of parental strain DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904
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WRM49 |
C. elegans |
mex-3(spr6[*tn1753]) I. Show Description
Homozygous fertile. mex-3(spr6) is a CRISPR/Cas9 engineered deletion removing 142 bp of the mex-3 3´UTR. Derived by modification of parental strain DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904
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WRM50 |
C. elegans |
mex-3(spr7[*tn1753]) I. Show Description
Homozygous fertile. mex-3(spr7) is a CRISPR/Cas9 engineered deletion removing 134 bp of the mex-3 3´UTR. Derived by modification of parental strain DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904
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WRM52 |
C. elegans |
mex-3(spr9[*tn1753]) I. Show Description
Homozygous fertile; reduced brood size. mex-3(spr9) is a CRISPR/Cas9 engineered deletion of the mex-3 3´UTR (removes 624 of 689 bp). Derived by modification of parental strain DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904
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WRM53 |
C. elegans |
mex-3(spr10[*tn1753]) I. Show Description
Homozygous fertile; reduced brood size. mex-3(spr5) is a CRISPR/Cas9 engineered deletion removing 190 bp of the mex-3 3´UTR. Derived by modification of parental strain DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904
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WS1433 |
C. elegans |
hus-1(op241) I; unc-119(ed3) III; opIs34. Show Description
opIs34 [hus-1p::hus-1::GFP + unc-119(+)]. Low-copy integrated array of 1144 bp hus-1 promoter and genomic coding sequence fused to GFP. Nuclear expression of GFP in germ cells and embryos. Integrated GFP construct completely rescues DNA damage induced cell cycle arrest defect and partially rescues DNA damage induced apoptosis of hus-1(op241).
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WS1609 |
C. elegans |
unc-69(ok339)/qC1 [dpy-19(e1259) glp-1(q339) III. Show Description
Heterozygotes are WT and segregate WT, Dpy Steriles and arrested L1 coilers. Fails to complement unc-50.
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WS1642 |
C. elegans |
rpoa-2(op259) I. Show Description
Maintain at 15-20C. Sterile at 25C. Apoptosis variant. Small germ line.
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WS2170 |
C. elegans |
unc-119(ed3) III; opIs110 IV. Show Description
opIs110 [lim-7p::YFP::actin + unc-119(+)] IV. YFP::ACT-5 expressed in somatic sheath cells, marks pre-disc corpses.
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WS2265 |
C. elegans |
hus-1(op244) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Heterozygotes are WT GFP+ and segregate non-glowing hus-1 homozygotes and very rare homozygous hT2 glowing animals, and dead eggs. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and gut promoter driving GFP in the intestine. hus-1(op244) mutants from homozygous parents show an incompletely penetrant maternal effect embryonic lethality. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
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WS2277 |
C. elegans |
hus-1(op241) I. Show Description
Maintain at 15 or 20C. Mrt at 25C. Defective for DNA damage induced germ cell death and cell cycle arrest.
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WS2972 |
C. elegans |
gla-3(op216) I. Show Description
Sterile or nearly sterile. Extra programmed cell death in germline. Maintain at 20 C. Reference: Kritikou et al. 2006 Genes Dev 20(16):2279-92.
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WS2973 |
C. elegans |
gla-3(op212) I. Show Description
Sterile or nearly sterile. Extra programmed cell death in germline. Maintain at 20 C. Reference: Kritikou et al. 2006 Genes Dev 20(16):2279-92.
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WS2974 |
C. elegans |
gla-3(ep312) I. Show Description
Sterile or nearly sterile. Extra programmed cell death in germline. Maintain at 20 C. Reference: Kritikou et al. 2006 Genes Dev 20(16):2279-92.
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WS3455 |
C. elegans |
chk-2(gk212) V. Show Description
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WS3530 |
C. elegans |
mtm-1(op309) I. Show Description
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WS4274 |
C. elegans |
unc-119(ed3) III; opIs206. Show Description
opIs206 [hif-1p::hif-1(genomic)::GFP::hif-1 3'UTR + unc-119(+)]. Weak GFP signal in early embryonic stages (2-cell, 4-cell, 8-cell, etc.). Reference: Sendoel A, et al. Nature. 2010 Jun 3;465(7298):577-83.
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WS4543 |
C. elegans |
opIs257. Show Description
opIs257 [rad-54p::rad-54::YFP::rad-54 3'UTR + unc-119(+)].
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WS4581 |
C. elegans |
unc-119(ed3) III; opIs263. Show Description
opIs263 [rpa-1p::rpa-1::YFP + unc-119(+)]. YFP expression in soma and germline.
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WS4591 |
C. elegans |
unc-130(op459) II; hse-5(tm472) III. Show Description
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WS4700 |
C. elegans |
opIs295 II; unc-119(ed3) III. Show Description
opIs295 [cdc-42p::GFP::cdc-42(genomic)::cdc-42 3'UTR + unc-119(+)] II.
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WS4918 |
C. elegans |
opIs310. Show Description
opIs310 [ced-1p::YFP::act-5::let-858 3'UTR + unc-119(+)].
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WS4920 |
C. elegans |
ced-2(n1994) IV; opIs310. Show Description
opIs310 [ced-1p::YFP::act-5::let-858 3'UTR + unc-119(+)].
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WS5018 |
C. elegans |
cdc-42(gk388) opIs295 II. Show Description
opIs295 [cdc-42p::GFP::cdc-42(genomic)::cdc-42 3'UTR + unc-119(+)] II. opIs295 fully rescues cdc-42 animals.
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WS5229 |
C. elegans |
unc-119(ed3) III; fan-1(tm423) IV; opIs406. Show Description
opIs406 [fan-1p::fan-1::GFP::let-858 3'UTR + unc-119(+)]. opIs406 resuces tm423. Reference: Kratz K, et al. Cell. 2010 Jul 9;142(1):77-88.
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WS5235 |
C. elegans |
ccz-1(t2129) V/nT1 [qIs51] (IV;V). Show Description
Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ccz-1 homozygotes (produce only arrested embryos with spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7.
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WS841 |
C. elegans |
ptp-2(op194) unc-4(e120)/mIn1 [dpy-10(e128)] II; him-5(e1490) V. Show Description
Heterozygotes are WT and segregate WT, Uncs which are sterile (>10 offspring) and Dpys. Throws males of all classes. mIn1 pka mC6.
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WT30 |
C. elegans |
unc-119(ed3) III; wtEx30. Show Description
wtEx30 [cuti-1p::cuti-1::GFP + unc-119(+)]. Maintain by picking non-Unc. Reference: Fritz JA & Behm CA. PLoS One. 2009;4(4):e5117.
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