| SD1891 |
C. elegans |
unc-119(ed3) III; wgIs203. Show Description
wgIs203 [nhr-76::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. Altered social behavior at 20C. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Gerstein MB, et al. Science. 2010 Dec 24;330(6012):1775-87. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
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| SD1894 |
C. elegans |
gaIs286. Show Description
gaIs286 [unc-62(7b)::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. A STOP-codon was inserted into exon 7a of unc-62 to generate an UNC-62(7b)-specific reporter. Recombineered fosmid was integrated by biolistic bombardment to produce strain OP602, which wa outcrossed to produce SD1894. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Gerstein MB, et al. Science. 2010 Dec 24;330(6012):1775-87. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
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| SD1897 |
C. elegans |
glo-4(ok623) V; wgIs600. Show Description
wgIs600 [unc-62::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. glo-4(ok623) causes a a partially-penetrant Dpy phenotype. Derived from parental strains RB811 and SD1871.
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| SD1898 |
C. elegans |
glo-4(ok623) V; gaIs286. Show Description
gaIs286 [unc-62(7b)::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. A STOP-codon was inserted into exon 7a of unc-62 to generate an UNC-62(7b)-specific reporter. Recombineered fosmid was integrated by biolistic bombardment to produce strain OP602, which wa outcrossed to produce SD1894. glo-4(ok623) causes a a partially-penetrant Dpy phenotype. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Gerstein MB, et al. Science. 2010 Dec 24;330(6012):1775-87. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org). Derived from parental strains RB811 and SD1894.
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| SD1910 |
C. elegans |
glo-4(ok623) V; stIs10453. Show Description
stIs10453 [elt-2::TGF(7E1)::GFP::TY1::3xFLAG inserted into fosmid WRM0617dE06 as C-terminal protein fusion]. glo-4(ok623) causes a a partially-penetrant Dpy phenotype.
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| SD1949 |
C. elegans |
glo-4(ok623) V; gaIs290. Show Description
gaIs290 [elt-2::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. Reduced autofluorescence. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Recombineered fosmid was integrated by biolistic bombardment. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Mann F, et al. PLoS. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
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| SD1954 |
C. elegans |
rde-1(ne219) V; kzIs9; wgIs600. Show Description
kzIs9 [(pKK1260) lin-26p::NLS::GFP + (pKK1253) lin-26p::rde-1 + rol-6(su1006)]. Rollers. Hypodermis specific RNAi. wgIs600 [unc-62::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering.
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| SD1966 |
C. elegans |
glp-1(e2141) III; gaIs290. Show Description
gaIs290 [elt-2::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. Temperature-sensitive sterile. Maintain at 15C. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Recombineered fosmid was integrated by biolistic bombardment. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Mann F, et al. PLoS. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
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| SD1989 |
C. elegans |
rde-1(ne300) V; gaIs290. Show Description
gaIs290 [elt-2::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. RNAi-resistant. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Recombineered fosmid was integrated by biolistic bombardment. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Mann F, et al. PLoS. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
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| SHG1614 |
C. elegans |
puf-8(ust245[puf-8::GFP::3xFlag]) II. Show Description
GFP::3xFlag inserted into endogenous puf-8 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG1676 |
C. elegans |
drh-3(ust352[drh-3::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous drh-3 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Chen X, et al. Nat Commun. 2024 Jul 10;15(1):5799. doi: 10.1038/s41467-024-50027-3. PMID: 38987544.
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| SHG1679 |
C. elegans |
ekl-1(ust353[ekl-1::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous ekl-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Chen X, et al. Nat Commun. 2024 Jul 10;15(1):5799. doi: 10.1038/s41467-024-50027-3. PMID: 38987544.
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| SHG1682 |
C. elegans |
elli-1(ust354[elli-1::GFP::3xFlag]) IV. Show Description
GFP::3xFlag inserted into endogenous elli-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Chen X, et al. Nat Commun. 2024 Jul 10;15(1):5799. doi: 10.1038/s41467-024-50027-3. PMID: 38987544.
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| SHG1683 |
C. elegans |
egc-1(ust355[egc-1::GFP::3xFlag]) III. Show Description
GFP::3xFlag inserted into endogenous egc-1/c14b1.12 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Chen X, et al. Nat Commun. 2024 Jul 10;15(1):5799. doi: 10.1038/s41467-024-50027-3. PMID: 38987544.
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| SHG1687 |
C. elegans |
edc-3(ust642[3xFlag::mCherry::edc-3]) I. Show Description
3xFlag::mCherry inserted into endogenous edc-3 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Zhang Y, et al. Sci Rep. 2021 Oct 13;11(1):20359.doi: 10.1038/s41598-021-99919-0. PMID: 34645931.
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| SHG2040 |
C. elegans |
csr-1(ust371[3xFlag::GFP::csr-1]) IV. Show Description
3xFlag::GFP inserted into endogenous csr-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2152 |
C. elegans |
fbf-2(ust337[fbf-2::GFP::3xFlag]) II. Show Description
GFP::3xFlag inserted into endogenous fbf-2 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2207 |
C.elegans |
ddx-19(ust399[ddx-19::GFP::3xFlag]) II. Show Description
GFP::3xFlag inserted into endogenous ddx-19 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2238 |
C. elegans |
meg-3(ust407[meg-3::GFP::3xFlag]) X. Show Description
GFP::3xFlag inserted into endogenous meg-3 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2239 |
C. elegans |
car-1(ust408[GFP::car-1]) I. Show Description
3xFlag::GFP inserted into endogenous car-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2240 |
C. elegans |
dcap-1(ust409[dcap-1::GFP::3xFlag]) IV. Show Description
GFP::3xFlag inserted into endogenous dcap-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2241 |
C. elegans |
pab-1(ust410[pab-1::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous pab-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2242 |
C. elegans |
parn-1(ust411[parn-1::GFP::3xFlag]) V. Show Description
GFP::3xFlag inserted into endogenous parn-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2243 |
C. elegans |
ifet-1(ust412[ifet-1::GFP::3xFlag]) III. Show Description
GFP::3xFlag inserted into endogenous ifet-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2244 |
C. elegans |
henn-1(ust413[henn-1::GFP::3xFlag]) III. Show Description
GFP::3xFlag inserted into endogenous henn-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2328 |
C. elegans |
plp-1(ust432[plp-1::GFP::3xFlag]) IV. Show Description
GFP::3xFlag inserted into endogenous plp-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2329 |
C. elegans |
mina-1(ust433[mina-1::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous mina-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2330 |
C. elegans |
spn-4(ust434[spn-4::GFP::3xFlag]) V. Show Description
GFP::3xFlag inserted into endogenous spn-4 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2331 |
C. elegans |
vbh-1(ust435[vbh-1::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous vbh-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2333 |
C. elegans |
pgl-3(ust437[pgl-3::GFP::3xFlag]) V. Show Description
GFP::3xFlag inserted into endogenous pgl-3 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2361 |
C. elegans |
mip-2(ust450[mip-2::GFP::3xFlag]) V. Show Description
GFP::3xFlag inserted into endogenous mip-2 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2362 |
C. elegans |
gld-1(ust451[gld-1::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous gld-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2427 |
C. elegans |
ife-1(ust446[ife-1::GFP::3xFlag]) III. Show Description
GFP::3xFlag inserted into endogenous ife-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2428 |
C. elegans |
mut-2(ust447[mut-2::GFP::3xFlag]) I. Show Description
3xFlag::GFP inserted into endogenous mut-2 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2429 |
C. elegans |
glh-2(ust448[glh-2::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous glh-2 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2430 |
C. elegans |
glh-4(ust449[glh-4::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous glh-4 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2534 |
C. elegans |
laf-1(ust492[laf-1::GFP::3xFlag]) III. Show Description
GFP::3xFlag inserted into endogenous laf-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2536 |
C. elegans |
glh-3(ust494[sxFlag::GFP::glh-3]) I. Show Description
3xFlag::GFP inserted into endogenous glh-3 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2537 |
C. elegans |
gld-3(ust495[gld-3::GFP::3xFlag]) II. Show Description
GFP::3xFlag inserted into endogenous gld-3 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2538 |
C. elegans |
deps-1(ust496[deps-1::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous deps-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2540 |
C. elegans |
gld-4(ust498[gld-4::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous gld-4 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2606 |
C. elegans |
pgl-2(ust515[3xFlag::GFP::pgl-2]) III. Show Description
3xFlag::GFP inserted into endogenous pgl-2 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2607 |
C. elegans |
pid-4(ust516[pid-4::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous pid-4 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2608 |
C. elegans |
pid-5(ust517[pid-5::GFP::3xFlag]) V. Show Description
GFP::3xFlag inserted into endogenous pid-5 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2609 |
C. elegans |
gls-1(ust518[glh-1::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous gls-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2672 |
C. elegans |
gld-2(ust546[gld-2::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous gld-2 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2701 |
C. elegans |
nhl-2(ust586[3xFlag::GFP::nhl-2]) III. Show Description
GFP::3xFlag inserted into endogenous nhl-2 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2875 |
C. elegans |
D2005.4(ust638)[D2005.4::GFP::3xFlag] I. Show Description
GFP::3xFlag inserted into endogenous D2005.4 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2876 |
C. elegans |
egc-3(ust620[egc-3::GFP::3xFlag]). Show Description
GFP::3xFlag inserted into endogenous egc-3(F59G1.8) locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2882 |
C. elegans |
rde-12(ust575[3xFlag::GFP::rde-12]) V. Show Description
3xFlag::GFP inserted into endogenous rde-12 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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