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Strain Species Genotype
KWN117 C. elegans pha-1(e2123) III; him-5(e1490) V; rnyEx60. Show Description
rnyEx60 [vha-6p:::vha-6::mCherry + myo-3p::GFP + pha-1(+)]. Maintain at 25C to select for animals carrying the array. Labels apical intestinal membrane. Reference: Allman et al. (2009) Am J Physiol Cell 297:C1071-81.
KWN190 C. elegans pha-1(e2123) III; him-5(e1490) V; rnyEx109. Show Description
rnyEx109 [nhx-2p::D3cpv + pha-1(+)]. Maintain at 20-25C to select for array. KWN190 strain expresses the FRET-based calcium indicator protein D3cpv throughout the cytoplasm of intestinal cells. Dual emission ratio imaging (ex. 435, em. 480/535-nm) can be used to measure intestinal calcium and, although the FRET pair is CFP/YFP, intestinal D3cpv fluorescence is observable under standard GFP filter sets. The D3cpv biosensor has the advantages of being relatively pH-insensitive and not interfering with endogenous calmodulin signaling. References: Am J Physiol Cell Physiol. 301:C1389-1403. Am J Physiol Cell Physiol. 297:C1071-81. FASEB J. 27:760-768. PLoS Biol. 11: e1001613.
MH1157 C. elegans him-5(e1490) V; egl-13(ku194) X. Show Description
ku194 is a loss of function allele, likely to be molecular null. Connection of gonad defective, >95% Egl. Anchor cell and uterine seam cell do not fuse. Males can mate. Hermaphrodites are very difficult to mate. Previously called cog-2(ku194).
MH1946 C. elegans dli-1(ku266)/+ IV; him-5(e1490) V. Show Description
Heterozygotes are WT and segregate WT and Sterile animals with a protruding vulva. Throws males. Clone several WT to recover heterozygote. Cytoplasmic dynein light intermediate chain. 9/02: ku266 is not an L to stop; it is a W117 to a stop (TGG to TGA). Sandhya Koushika.
MOS370 C. elegans him-5(e1490) V; lite-1(ce314) X; fsIs22; ljIs114. Show Description
fsIs22 [osm-5p::fem-3::mCherry + unc-122p::GFP]. ljIs114 [gpa-13p?FLPase + sra-6p?FRT::mCherry::StopCodon::FRT?ChR2?YFP]. Him. Pan-ciliated masculinization with ChR2 specifically in ASH. Reference: Pechuk V., et al. 2022, Current Biology 32, 1–14. https://doi.org/10.1016/j.cub.2022.08.038
MOS374 C. elegans him-5(e1490) V; etyEx106. Show Description
etyEx106 [sra-6p::Cx36::YFP + flp-18p::Cx36::mCherry + unc-122p::GFP]. Pick animals with GFP+ coelomocytes to maintain. Artificial gap-junction between ASH and AVA. Reference: Pechuk V., et al. 2022, Current Biology 32, 1–14. https://doi.org/10.1016/j.cub.2022.08.038
MOS88 C. elegans him-5(e1490) V; etyIs1. Show Description
etyIs1 [gpa-13p::FLPase + sra-6p::FRT::mCherry::StopCodon::FRT::GCaMP6s]. Him. Integrated ASH-specific calcium sensor transgene. Reference: Pechuk V., et al. 2022, Current Biology 32, 1–14. https://doi.org/10.1016/j.cub.2022.08.038
MT2537 C. elegans dpy-13(e184) unc-8(n491) IV; him-5(e1490) V. Show Description
DpyUnc. e184 and n491 are both semi-dominant.
MT4051 C. elegans lin-44(n1792) I; him-5(e1490) V. Show Description
At 20C about 50% of the hermaphrodites are Egl. Males have an abnormal tail and do not mate.
MT4150 C. elegans unc-17(e245) dpy-4(e1166) IV; him-5(e1490) V. Show Description
DpyUnc. Segregates males.
MT4156 C. elegans lin-26(n156)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-5(e1490) V. Show Description
Heterozygotes are WT and segregate WT, Egls with abnormal tails and DpyUncs.
MT4306 C. elegans lin-39(n1490) unc-36(e251) III. Show Description
Unc. Vulvaless (temperature sensitive-91% Vul at 15C and 100% Vul at 20C and 25C). n1490 is also temperature sensitive Unc.
MT4700 C. elegans let-60(n1531) unc-22(e66)/let-60(n1700) dpy-20(e1282) IV; him-5(e1490) V. Show Description
Heterozygotes are mostly non-Muv (about 20% will be Muv because n1700 is semi-dominant) and non-Unc. Hets segregate Twitchers which die at various larval stages or as Vul adults, because of the maternal effect rescue of n1531 by n1700. The Twitchers will not give viable progeny. Hets also segregate DpyMuvs. The strain segregates males which will mate, though not very well.
MT4705 C. elegans lin-17(n671) lin-44(n1792) I; him-5(e1490) V. Show Description
MT4718 C. elegans egl-31(n472) I; him-5(e1490) V. Show Description
Egg laying defective. Makes bags of worms. Backward Unc. Throws males.
MT4837 C. elegans sma-3(e491) lin-39(n1490) III. Show Description
Small. Vulvaless (temperature sensitive-91% Vul at 15C and 100% Vul at 20C and 25C). n1490 is also temperature sensitive Unc.
MT5726 C. elegans dpy-20(e1362) unc-22(e66) IV; him-5(e1490) V; nDp5 (IV;f). Show Description
Animals with the Duplication are WT. Animals which have lost the Duplication are Dpy and Twitchers. Throws both WT and DpyTwitcher males. Maintain by picking WT.
MT5823 C. elegans lin-26(n156) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-5(e1490) V. Show Description
Heterozygotes are WT.
NC3540 C. elegans otIs458. Show Description
otIs458[ceh-63p::GFP + unc-119(+)]. GFP expression in DVC neurons. Can be used to isolate DVC by FACS. Derived by outcrossing parental strain OH11974 to remove him-5(e1490). Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
NG2517 C. elegans him-5(e1490) V; gmIs5. Show Description
gmIs5 [ina-1::GFP + rol-6(su1006)]. Rollers. ina-1(gm86) may still be in the background.
NM5176 C. elegans jsTi1490 IV. Show Description
jsTi1490 [LoxP::mex-5p::FLP::SL2::mNeonGreen::rpl-28p::FRT::GFP::his-58::FRT3] IV. jsTi1490 is an RMCE landing site inserted using miniMos located on Chr IV at 7,310,985 (at 3.32 m.u.) between glr-4 and F42C5.8. Insertion site gtacataaattataccaaatattgaTAaaagctacgaaaattccactgatat with rpl-28 transcription towards F42C5.8. Reference: Nonet ML. Genetics. 2020.
NW1550 C. elegans efn-2(ev658) him-5(e1490) Show Description
Male ray fusions: approx 15% penetrant.
NW1615 C. elegans plx-1(ev724) jcIs1 IV; him-5(e1490) V. Show Description
jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
NW1696 C. elegans him-5(e1490) V; evIs138. Show Description
evIs138 [plx-2(+) + sur-5::GFP]. Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1697 C. elegans plx-2(ev775) II; him-5(e1490) V. Show Description
Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1698 C. elegans plx-2(ev774) II; him-5(e1490) V. Show Description
Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1699 C. elegans plx-2(ev738) II; him-5(e1490) V. Show Description
Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1700 C. elegans plx-2(ev773) II; him-5(e1490) V. Show Description
Null allele. Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single. Some embryonic and larval lethality.
NW1701 C. elegans mab-20(ev778) I; muIs32 II; him-5(e1490) V. Show Description
muIs32 [mec-7p::GFP + lin-15(+)]. Extensive ray fusion. Body morphology defects at larval stages. Embryonic and larval lethality.
NW1702 C. elegans smp-1(ev715) I; jcIs1 IV; him-5(e1490) V. Show Description
jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Strain does not Roll but otherwise seems fine. Male ray 1 anterior displacement in homozygous animals. Genetic interaction with smp-2(ev709). Vulva cell migration defects. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
NW1703 C. elegans him-5(e1490) V; evEx193. Show Description
evEx193 [semala::GFP]. GFP fluorescence in interneurons (AVL), the excretory channel, the distal tip cells (DTC), somatic cells of the gonad, epidermal cells. In adult, expression in the fused seam cell syncitium.
NW1704 C. elegans smp-2(ev709) I; jcIs1 IV; him-5(e1490) V. Show Description
jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Strain does not Roll but otherwise seems fine. Male ray 1 anterior displacement in homozygous animals. Genetic interaction with smp-1(ev715). ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
NY2045 C. elegans ynIs45 I; him-5(e1490) V. Show Description
ynIs45 [flp-15p::GFP].
NY2054 C. elegans ynIs54 IV; him-5(e1490) V. Show Description
ynIs54 [flp-20p::GFP]. Him.
NY2057 C. elegans ynIs57 III; him-5(e1490) V. Show Description
ynIs57 [flp-2p::GFP].
NY2064 C. elegans ynIs64 I; him-5(e1490) V. Show Description
ynIs64 [flp-17p::GFP].
NY2066 C. elegans ynIs66 IV; him-5(e1490) V. Show Description
ynIs66 [flp-7p::GFP].
NY2067 C. elegans ynIs67 III; him-5(e1490) V. Show Description
ynIs67 [flp-6p::GFP].
OH10196 C. elegans ceh-43(tm480) III; him-5(e1490) V; nIs118; norEx41. Show Description
nIs118 [cat-2::GFP]. norEx41 [ceh-43 fosmid + dat-1::mCherry]. Him. tm480 embryonic lethality is rescued by extrachromosomal array. Pick mCherry+ animals to maintain.
OH10198 C. elegans ceh-43(tm480) III; him-5(e1490) V; otIs226; norEx41. Show Description
otIs226 [bas-1::GFP]. norEx41 [ceh-43 fosmid + dat-1::mCherry]]. Him. tm480 embryonic lethality is rescued by extrachromosomal array. Pick mCherry+ animals to maintain.
OH10235 C. elegans ceh-43(tm480) III; him-5(e1490) V; bxIs19; norEx41. Show Description
bxIs19 [trp-4p::GFP]. norEx41 [ceh-43 fosmid + dat-1::mCherry]. Him. tm480 embryonic lethality is rescued by extrachromosomal array. Pick mCherry+ animals to maintain.
OH12312 C. elegans otIs388 pha-1(e2123) III; him-5(e1490) V. Show Description
otIs388 [eat-4(fosmid)::SL2::YFP::H2B + pha-1(+)] III. Him. Reference: Serrano-Saiz E, et al. Cell. 2013 Oct 24;155(3):659-73.
OH127 C. elegans him-5(e1490) V; otIs14. Show Description
otIs14 [zig-3::GFP + rol-6(su1006)]. Rollers.
OH128 C. elegans otIs39 II; him-5(e1490) V. Show Description
otIs39 [unc-47(delta)::GFP + lin-15(+)]. Expressed in RMEL/R, AVL, RIS, DVB, PVT and unidentified amphid sensory neuron pair.
OH13083 C. elegans him-5(e1490) V; otIs576. Show Description
otIs576 [unc-17(fosmid)::GFP + lin-44::YFP]. Him. GFP expression in all cholinergic neurons. Reference: Pereira L, et al. Elife. 2015 Dec 25;4.
OH13104 C. elegans him-5(e1490) V; otIs565. Show Description
otIs565 [unc-47(fosmid)::SL2::H2B::mChopti + pha-1(+)]. Reporter tag inserted into fosmid WRM0626bG04. Him. Line 5-1. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5.
OH13105 C. elegans him-5(e1490) otIs564 V. Show Description
otIs564 [unc-47(fosmid)::SL2::H2B::mChopti + pha-1(+)] V. Reporter tag inserted into fosmid WRM0626bG04. Him. Line 2-2. Inserted near unc-42. Brighter mCherry expression than in OH13104. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5.
OH13106 C. elegans him-5(e1490) V; otIs568. Show Description
otIs568 [unc-46(fosmid)::SL2::H2B::mChopti + pha-1(+)]. Reporter tag inserted into fosmid WRM0611dE07. Him. Line 19-2. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5.
OH13107 C. elegans otIs570 I; him-5(e1490) V. Show Description
otIs570 [snf-11(fosmid)::SL2::H2B::mChopti + pha-1(+)] I. Reporter tag inserted into fosmid WRM064dH02. Him. Line 14-1. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5.
OH13333 C. elegans him-5(e1490) V; otIs514. Show Description
otIs514 [unc-25p::unc-25(partial)::GFP::unc-54 3'UTR + pha-1(+)]. Him. Reporter contains 1.8 kb upstream of the unc-25 start codon through exon 6. Derived from injection of pMG89; line 14-12. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5.