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Strain Species Genotype
AD271 C. elegans spe-38(eb44) I; him-5(e1490) V; asEx78. Show Description
asEx78 [spe-38p::spe-38(cDNA)::spe-38 3'UTR + myo-3p::GFP]. Pick GFP+ to maintain. GFP+ worms are fertile; animals that have lost the array are sterile.
AD281 C. elegans spe-45(as38) IV; him-5(e1490) V. Show Description
Him. Temperature-sensitive sterile. Small brood size even at permissive temperatures; pick fertile animals and maintain at 15C. Worms lacking spe-45 function produce morphologically normal and motile sperm that cannot fuse with oocytes despite direct contact in the reproductive tract. spe-45 hermaphrodites and males are subfertile at 16C and sterile at 25C. Reference: Singaravelu G, et al. Current Biology 2015. http://dx.doi.org/10.1016/j.cub.2015.10.055
AD292 C. elegans spe-51(as39) IV; him-5(e1490) V; asEx95. Show Description
asEx95 [T22B11.1(genomic) + myo-3p::GFP]. Pick GFP+ animals to maintain. as39 is a non-conditional allele of spe-51. Mutant hermaphrodites and males are severely subfertile due to a sperm defect. The extrachromosomal array asEx95 effectively rescues the fertility defect. Him. Reference: Mei X, et al. Curr Biol. 2023 Jul 3;S0960-9822(23)00780-7. doi: 10.1016/j.cub.2023.06.029. PMID: 37453427.
AD294 C. elegans cylc-2(mon2[cylc-2::mNG::3xFLAG) I; him-5(e1490) V. Show Description
Endogenous cycl-2 locus tagged with mNeonGreen (mNG). Green fluorescence in sperm. Him. Reference: Krauchunas AR, et al. (2020). C. elegans CYLC-2 localizes to sperm. microPublication Biology. 10.17912/micropub.biology.000314.
AD296 C. elegans spe-51(syb2737[spe-51::mNeonGreen]) IV; him-5(e1490) V. Show Description
mNeonGreen tag inserted into the endogenous spe-51 locus. Him. Reference: Mei X, et al. Curr Biol. 2023 Jul 3;S0960-9822(23)00780-7. doi: 10.1016/j.cub.2023.06.029. PMID: 37453427.
AD319 C. elegans spe-38(syb6556[spe-38::wrmScarlet-I]) I; him-5(e1490) V. Show Description
wrmScarlet-I tag inserted into endogenous spe-38 locus. Him. wrmScarlet-I expression labels membranous organelles (MOs) in the sperm. Reference: Zuo Y, et al. Biomolecules. 2023; 13(4):623. https://doi.org/10.3390/biom13040623
ARK7 C. elegans spe-36(nwk1[spe-36::gfp]) IV; him-5(e1490) V. Show Description
GFP tag inserted into endogenous spe-36 (F40F11.4) locus. SPE-36::GFP expression in spermatids and spermatozoa. Reference: Krauchunas AR, et al. Curr Biol. 2023 Jul 24;33(14):3056-3064.e5. doi: 10.1016/j.cub.2023.06.051. PMID: 37453426.
AV285 C. elegans swm-1(me66) him-5(e1490) V. Show Description
Sperm activation in virgin males. Poor sperm transfer.
AV322 C. elegans swm-1(me87) him-5(e1490) V. Show Description
Sperm activation in virgin males. Very poor sperm transfer.
BA1044 C. elegans spe-27(it132) spe-29(it127) IV; him-5(e1490) V. Show Description
Non-conditional hermaphrodite sterile. Males fertile. Self-progeny produced after mating.
BA1051 C. elegans spe-12(hc76) I; spe-29(it127) IV; him-5(e1490) V. Show Description
Self-sterile hermaphrodites; fertile males. Mated hermaphrodites produce self and outcross progeny. Self progeny are 33% males.
BA524 C. elegans fer-1(hc1) I; him-5(e1490) V. Show Description
Temperature sensitive. Maintain at 15C. Throws males.
BA547 C. elegans fer-2(hc2) III; him-5(e1490) V. Show Description
Fertile at 16c and 20C. Sterile at 25C-Produces some defective embryos and 0-5 progeny. Throws males.
BA562 C. elegans fer-4(hc4) him-5(e1490) V. Show Description
Temperature sensitive. Maintain at 15C. Produces about 30% males.
BA782 C. elegans spe-10(hc104) him-5(e1490) V. Show Description
Temperature sensitive, maintain at 16C. Segregates males. Average progeny at 16C is 7 +/- 5. Average progeny at 25C is 0.1 +/- .1.
BC3448 C. elegans dpy-18(e364)/eT1 III; let-428(s1490) unc-46(e177)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal late larval-Extrudes viscera. Maintain by picking WT.
BC4923 C. elegans egl-38(sy294) IV; him-5(e1490) V. Show Description
BW1809 C. elegans gpa-16(it143) I; him-5(e1490) V. Show Description
Temperature-sensitve. Maintain at 15C. Slight Maternal Effect Lethal (Mel) at 15C, more pronounced at 20C. Highly penetrant Mel at 25C and a fraction of the survivors have reversed left-right organs.
BW1935 C. elegans unc-119(ed3) III; ctIs43 him-5(e1490) V. Show Description
ctIs43 [dbl-1p::GFP + dbl-1p::GFP::NLS + unc-119(+)].
CB1490 C. elegans ali-1(e1934) him-5(e1490) V. Show Description
Faint or invisible alae in L1. Segregates males.
CB2338 C. elegans mab-6(e1249) II; him-5(e1490) V. Show Description
Segregates males. Males abnormal.
CB2810 C. elegans tra-1(e1575)/+ III; unc-42(e270) him-5(e1490) dpy-21(e428) V. Show Description
Pick Unc hermaphrodite to maintain (these will be tra-1/+; unc-42 him-5 dpy-21 XO animals). Segregates Unc hermaphrodites (tra-1/+ III), Unc males (+/+ III), and DpyUnc hermaphrodites (+/+ III). dpy-21 not expressed in XO. dpy-21(e428) V; XX animals are weak Dpy. dpy-21(e428) V; XO animals are non-Dpy.
CB3252 C. elegans rnt-1(e1241) I; him-5(e1490) dpy-21(e428) V. Show Description
Hermaphrodites are Dpy and Him. Males are non-Dpy and Mab. Males have abnormal bursa and defective rays. Both sexes show lateral hypodermis lineage defexts. M-MATING+POOR <1%WT.
CB3297 C. elegans vab-9(e1744) II; him-5(e1490) V. Show Description
Him. M-MATING+POOR <1%WT. Tail abnormalities->knob-like swelling in larvae and adults.
CB3298 C. elegans him-5(e1490) dpy-21(e428) V; mab-7(e1599) X. Show Description
Hermaphrodites are Dpy. Males are non-Dpy and have abnormal bursae in adult, with swollen rays. Males will mate, but with very low efficiency. [3/98: King Chow isolated a line from the CGC stock that was throwing 100% Mabs. Sent the strain back to the CGC to replace the old stock.]
CB3299 C. elegans mab-5(e1239) III; him-5(e1490) dpy-21(e428) V. Show Description
Hermaphrodites are Dpy and segregate males. Males are non-Dpy and Mab. Both sexes show lineage alterations.
CB3353 C. elegans mab-9(e1245) II; him-5(e1490) dpy-21(e428) V. Show Description
Hermaphrodites are Dpy and segregates males. Males are non-Dpy and have severe tail abnormalities; frequently lethal to adult males.
CB3518 C. elegans mab-10(e1248) II; him-5(e1490) V. Show Description
Hermaphrodtes segregate impotent males. Males show very slight swelling of bursa.
CB3531 C. elegans mab-5(e1239) III; him-5(e1490) V. Show Description
Males abnormal.
CB3855 C. elegans plg-1(e2001) III; him-5(e1490) V. Show Description
Hermaphrodites and males superficially WT. Males lay down a gelatinous plug over the vulva of mated hermaphrodites. Plug formation controlled only by male genotype. Both plg-1 and plg-1/+ males make plugs.
CB3924 C. elegans pal-1(e2091) III; him-5(e1490) V. Show Description
Males are missing most of the rays and additional alae extend into the tail.
CB4043 C. elegans smg-2(e2008) I; him-5(e1490) V. Show Description
e2008 recessively suppresses the phenotypes of mutations in unc-54(r293), lin-29(n546), tra-2 and dpy-5. Abnormal male tail. smg-2 homozygotes have slightly abnormal movement.
CB4088 C. elegans him-5(e1490) V. Show Description
From CB1490, which is a double mutant of him-5 with ali-1. CB4088 has been outcrossed to remove ali-1.
CB4104 C. elegans mab-12(e2166) IV; him-5(e1490) V. Show Description
Males have abnormal swollen bursa. Hermaphrodites have abnormal swollen vulva.
CB4111 C. elegans mab-24(e2169) I; him-5(e1490) V. Show Description
Anterior displacement of rays and fan. Ray identities are not altered. Slightly Unc (coiler).
CB4219 C. elegans pal-2(e2260) IV; him-5(e1490) V. Show Description
WT hermaphrodites. Mails have ectopic lateral alae in tail region. Segregates males.
CB4354 C. elegans smg-3(ma117) IV; him-5(e1490) V. Show Description
Hermaphrodites have an abnormal protrusive vulva. Segregate males with abnormally swollen bursa. Suppressor of certain alleles of certain genes.
CB4377 C. elegans lin-22(n372) pal-2(e2260) IV; him-5(e1490) V; lin-32(e1926) X. Show Description
Hermaphrodites segregating males with abnormal ray lineages. Reference: Hodgkin et al. (1989) Genome, 31, 625-637. doi:10.1139/g89-116.
CB4554 C. elegans smg-2(e2008) smg-1(e1228) I; him-5(e1490) V. Show Description
Smg (protruding vulva) hermaphrodites segregating Smg hermaphrodites and Smg (Male abnormal) males. Reference: Hodgkin et al. (1989) PMID: 2583479.
CB4605 C. elegans mab-9(e2410) II; him-5(e1490) V. Show Description
CB5365 C. elegans tra-3(bn75) IV; him-5(e1490) V. Show Description
Him. Temperature-sensitive: infertile above 20C. Variably masculinized XX hermaphrodites segregating normal XO males. Stronger XX masculinization than null alleles of tra-3. Reference: Francis et al. (1995) PMID: 7713420.
CB6740 C. elegans bus-20(e2990) III; him-5(e1490) V. Show Description
Anatomically normal hermaphrodites and males; Bus (resistant to infection by M. nematophilum); weakly resistant to Leucobacter Verde2; insensitive to Leucobacter Verde1. Reference: J Hodgkin (unpublished).
CB6742 C. elegans dhs-5(e2992) II; him-5(e1490) V. Show Description
Bus (resistant to M. nematophilum infection); sensitive to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: M J Gravato-Nobre, J Hodgkin (unpublished).
CB7109 C. elegans acs-20(e3031) IV; him-5(e1490) V. Show Description
Him. Skiddy, bleach-sensitive hermaphrodites and males. Resistant to Leucobacter Verde1. Reference: O'Rourke et al. (in preparation).
CB7148 C. elegans him-5(e1490) V; bus-8B(tm1410) X; eEx763. Show Description
eEx763 [bus-8(exonU2stop) + sur-5p::GFP]. Pick GFP+ to maintain. Lethal bus-8 mutation rescued by bus-8 transgene defective in 5' exons U1 and U2. Reference: Stroud et al (in preparation).
CB7425 C. elegans him-5(e1490) V; bus-25(e3094) X. Show Description
Him. Small, slow-growing, bleach-sensitive; Bus (resistant to M. nematophilum), slightly resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: Hodgkin et al (in preparation).
CF12 C. elegans rol-6(e187) II; lin-22(n372) IV; him-5(e1490) V. Show Description
Rollers. lin-22 and him-5 mutations affect neuroblast formation from epidermal precursor cell V5.
CF250 C. elegans cat-4(e1141) him-5(e1490) V. Show Description
Catecholamine abnormal. Throws males.
CF26 C. elegans lin-22(mu2) IV; him-5(e1490) V. Show Description
Alae-to-ray transformation similar to that of n372 animals.
CF301 C. elegans mab-5(e2088) III; unc-31(e169) IV; him-5(e1490) V; muIs9 X. Show Description
muIs9 [hs-mab-5 + C14G10]. Heat-shock inducible mab-5. C14G10 contains a WT copy of unc-31. muIs9 integrated by gamma irradiation.