AD271 |
C. elegans |
spe-38(eb44) I; him-5(e1490) V; asEx78. Show Description
asEx78 [spe-38p::spe-38(cDNA)::spe-38 3'UTR + myo-3p::GFP]. Pick GFP+ to maintain. GFP+ worms are fertile; animals that have lost the array are sterile.
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AD281 |
C. elegans |
spe-45(as38) IV; him-5(e1490) V. Show Description
Him. Temperature-sensitive sterile. Small brood size even at permissive temperatures; pick fertile animals and maintain at 15C. Worms lacking spe-45 function produce morphologically normal and motile sperm that cannot fuse with oocytes despite direct contact in the reproductive tract. spe-45 hermaphrodites and males are subfertile at 16C and sterile at 25C. Reference: Singaravelu G, et al. Current Biology 2015. http://dx.doi.org/10.1016/j.cub.2015.10.055
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AD292 |
C. elegans |
spe-51(as39) IV; him-5(e1490) V; asEx95. Show Description
asEx95 [T22B11.1(genomic) + myo-3p::GFP]. Pick GFP+ animals to maintain. as39 is a non-conditional allele of spe-51. Mutant hermaphrodites and males are severely subfertile due to a sperm defect. The extrachromosomal array asEx95 effectively rescues the fertility defect. Him. Reference: Mei X, et al. Curr Biol. 2023 Jul 3;S0960-9822(23)00780-7. doi: 10.1016/j.cub.2023.06.029. PMID: 37453427.
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AD294 |
C. elegans |
cylc-2(mon2[cylc-2::mNG::3xFLAG) I; him-5(e1490) V. Show Description
Endogenous cycl-2 locus tagged with mNeonGreen (mNG). Green fluorescence in sperm. Him. Reference: Krauchunas AR, et al. (2020). C. elegans CYLC-2 localizes to sperm. microPublication Biology. 10.17912/micropub.biology.000314.
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AD296 |
C. elegans |
spe-51(syb2737[spe-51::mNeonGreen]) IV; him-5(e1490) V. Show Description
mNeonGreen tag inserted into the endogenous spe-51 locus. Him. Reference: Mei X, et al. Curr Biol. 2023 Jul 3;S0960-9822(23)00780-7. doi: 10.1016/j.cub.2023.06.029. PMID: 37453427.
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AD319 |
C. elegans |
spe-38(syb6556[spe-38::wrmScarlet-I]) I; him-5(e1490) V. Show Description
wrmScarlet-I tag inserted into endogenous spe-38 locus. Him. wrmScarlet-I expression labels membranous organelles (MOs) in the sperm. Reference: Zuo Y, et al. Biomolecules. 2023; 13(4):623. https://doi.org/10.3390/biom13040623
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ARK7 |
C. elegans |
spe-36(nwk1[spe-36::gfp]) IV; him-5(e1490) V. Show Description
GFP tag inserted into endogenous spe-36 (F40F11.4) locus. SPE-36::GFP expression in spermatids and spermatozoa. Reference: Krauchunas AR, et al. Curr Biol. 2023 Jul 24;33(14):3056-3064.e5. doi: 10.1016/j.cub.2023.06.051. PMID: 37453426.
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AV285 |
C. elegans |
swm-1(me66) him-5(e1490) V. Show Description
Sperm activation in virgin males. Poor sperm transfer.
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AV322 |
C. elegans |
swm-1(me87) him-5(e1490) V. Show Description
Sperm activation in virgin males. Very poor sperm transfer.
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BA1044 |
C. elegans |
spe-27(it132) spe-29(it127) IV; him-5(e1490) V. Show Description
Non-conditional hermaphrodite sterile. Males fertile. Self-progeny produced after mating.
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BA1051 |
C. elegans |
spe-12(hc76) I; spe-29(it127) IV; him-5(e1490) V. Show Description
Self-sterile hermaphrodites; fertile males. Mated hermaphrodites produce self and outcross progeny. Self progeny are 33% males.
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BA524 |
C. elegans |
fer-1(hc1) I; him-5(e1490) V. Show Description
Temperature sensitive. Maintain at 15C. Throws males.
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BA547 |
C. elegans |
fer-2(hc2) III; him-5(e1490) V. Show Description
Fertile at 16c and 20C. Sterile at 25C-Produces some defective embryos and 0-5 progeny. Throws males.
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BA562 |
C. elegans |
fer-4(hc4) him-5(e1490) V. Show Description
Temperature sensitive. Maintain at 15C. Produces about 30% males.
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BA782 |
C. elegans |
spe-10(hc104) him-5(e1490) V. Show Description
Temperature sensitive, maintain at 16C. Segregates males. Average progeny at 16C is 7 +/- 5. Average progeny at 25C is 0.1 +/- .1.
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BC3448 |
C. elegans |
dpy-18(e364)/eT1 III; let-428(s1490) unc-46(e177)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal late larval-Extrudes viscera. Maintain by picking WT.
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BC4923 |
C. elegans |
egl-38(sy294) IV; him-5(e1490) V. Show Description
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BW1809 |
C. elegans |
gpa-16(it143) I; him-5(e1490) V. Show Description
Temperature-sensitve. Maintain at 15C. Slight Maternal Effect Lethal (Mel) at 15C, more pronounced at 20C. Highly penetrant Mel at 25C and a fraction of the survivors have reversed left-right organs.
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BW1935 |
C. elegans |
unc-119(ed3) III; ctIs43 him-5(e1490) V. Show Description
ctIs43 [dbl-1p::GFP + dbl-1p::GFP::NLS + unc-119(+)].
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CB1490 |
C. elegans |
ali-1(e1934) him-5(e1490) V. Show Description
Faint or invisible alae in L1. Segregates males.
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CB2338 |
C. elegans |
mab-6(e1249) II; him-5(e1490) V. Show Description
Segregates males. Males abnormal.
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CB2810 |
C. elegans |
tra-1(e1575)/+ III; unc-42(e270) him-5(e1490) dpy-21(e428) V. Show Description
Pick Unc hermaphrodite to maintain (these will be tra-1/+; unc-42 him-5 dpy-21 XO animals). Segregates Unc hermaphrodites (tra-1/+ III), Unc males (+/+ III), and DpyUnc hermaphrodites (+/+ III). dpy-21 not expressed in XO. dpy-21(e428) V; XX animals are weak Dpy. dpy-21(e428) V; XO animals are non-Dpy.
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CB3252 |
C. elegans |
rnt-1(e1241) I; him-5(e1490) dpy-21(e428) V. Show Description
Hermaphrodites are Dpy and Him. Males are non-Dpy and Mab. Males have abnormal bursa and defective rays. Both sexes show lateral hypodermis lineage defexts. M-MATING+POOR <1%WT.
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CB3297 |
C. elegans |
vab-9(e1744) II; him-5(e1490) V. Show Description
Him. M-MATING+POOR <1%WT. Tail abnormalities->knob-like swelling in larvae and adults.
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CB3298 |
C. elegans |
him-5(e1490) dpy-21(e428) V; mab-7(e1599) X. Show Description
Hermaphrodites are Dpy. Males are non-Dpy and have abnormal bursae in adult, with swollen rays. Males will mate, but with very low efficiency. [3/98: King Chow isolated a line from the CGC stock that was throwing 100% Mabs. Sent the strain back to the CGC to replace the old stock.]
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CB3299 |
C. elegans |
mab-5(e1239) III; him-5(e1490) dpy-21(e428) V. Show Description
Hermaphrodites are Dpy and segregate males. Males are non-Dpy and Mab. Both sexes show lineage alterations.
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CB3353 |
C. elegans |
mab-9(e1245) II; him-5(e1490) dpy-21(e428) V. Show Description
Hermaphrodites are Dpy and segregates males. Males are non-Dpy and have severe tail abnormalities; frequently lethal to adult males.
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CB3518 |
C. elegans |
mab-10(e1248) II; him-5(e1490) V. Show Description
Hermaphrodtes segregate impotent males. Males show very slight swelling of bursa.
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CB3531 |
C. elegans |
mab-5(e1239) III; him-5(e1490) V. Show Description
Males abnormal.
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CB3855 |
C. elegans |
plg-1(e2001) III; him-5(e1490) V. Show Description
Hermaphrodites and males superficially WT. Males lay down a gelatinous plug over the vulva of mated hermaphrodites. Plug formation controlled only by male genotype. Both plg-1 and plg-1/+ males make plugs.
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CB3924 |
C. elegans |
pal-1(e2091) III; him-5(e1490) V. Show Description
Males are missing most of the rays and additional alae extend into the tail.
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CB4043 |
C. elegans |
smg-2(e2008) I; him-5(e1490) V. Show Description
e2008 recessively suppresses the phenotypes of mutations in unc-54(r293), lin-29(n546), tra-2 and dpy-5. Abnormal male tail. smg-2 homozygotes have slightly abnormal movement.
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CB4088 |
C. elegans |
him-5(e1490) V. Show Description
From CB1490, which is a double mutant of him-5 with ali-1. CB4088 has been outcrossed to remove ali-1.
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CB4104 |
C. elegans |
mab-12(e2166) IV; him-5(e1490) V. Show Description
Males have abnormal swollen bursa. Hermaphrodites have abnormal swollen vulva.
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CB4111 |
C. elegans |
mab-24(e2169) I; him-5(e1490) V. Show Description
Anterior displacement of rays and fan. Ray identities are not altered. Slightly Unc (coiler).
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CB4219 |
C. elegans |
pal-2(e2260) IV; him-5(e1490) V. Show Description
WT hermaphrodites. Mails have ectopic lateral alae in tail region. Segregates males.
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CB4354 |
C. elegans |
smg-3(ma117) IV; him-5(e1490) V. Show Description
Hermaphrodites have an abnormal protrusive vulva. Segregate males with abnormally swollen bursa. Suppressor of certain alleles of certain genes.
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CB4377 |
C. elegans |
lin-22(n372) pal-2(e2260) IV; him-5(e1490) V; lin-32(e1926) X. Show Description
Hermaphrodites segregating males with abnormal ray lineages. Reference: Hodgkin et al. (1989) Genome, 31, 625-637. doi:10.1139/g89-116.
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CB4554 |
C. elegans |
smg-2(e2008) smg-1(e1228) I; him-5(e1490) V. Show Description
Smg (protruding vulva) hermaphrodites segregating Smg hermaphrodites and Smg (Male abnormal) males. Reference: Hodgkin et al. (1989) PMID: 2583479.
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CB4605 |
C. elegans |
mab-9(e2410) II; him-5(e1490) V. Show Description
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CB5365 |
C. elegans |
tra-3(bn75) IV; him-5(e1490) V. Show Description
Him. Temperature-sensitive: infertile above 20C. Variably masculinized XX hermaphrodites segregating normal XO males. Stronger XX masculinization than null alleles of tra-3. Reference: Francis et al. (1995) PMID: 7713420.
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CB6740 |
C. elegans |
bus-20(e2990) III; him-5(e1490) V. Show Description
Anatomically normal hermaphrodites and males; Bus (resistant to infection by M. nematophilum); weakly resistant to Leucobacter Verde2; insensitive to Leucobacter Verde1. Reference: J Hodgkin (unpublished).
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CB6742 |
C. elegans |
dhs-5(e2992) II; him-5(e1490) V. Show Description
Bus (resistant to M. nematophilum infection); sensitive to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: M J Gravato-Nobre, J Hodgkin (unpublished).
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CB7109 |
C. elegans |
acs-20(e3031) IV; him-5(e1490) V. Show Description
Him. Skiddy, bleach-sensitive hermaphrodites and males. Resistant to Leucobacter Verde1. Reference: O'Rourke et al. (in preparation).
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CB7148 |
C. elegans |
him-5(e1490) V; bus-8B(tm1410) X; eEx763. Show Description
eEx763 [bus-8(exonU2stop) + sur-5p::GFP]. Pick GFP+ to maintain. Lethal bus-8 mutation rescued by bus-8 transgene defective in 5' exons U1 and U2. Reference: Stroud et al (in preparation).
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CB7425 |
C. elegans |
him-5(e1490) V; bus-25(e3094) X. Show Description
Him. Small, slow-growing, bleach-sensitive; Bus (resistant to M. nematophilum), slightly resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: Hodgkin et al (in preparation).
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CF12 |
C. elegans |
rol-6(e187) II; lin-22(n372) IV; him-5(e1490) V. Show Description
Rollers. lin-22 and him-5 mutations affect neuroblast formation from epidermal precursor cell V5.
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CF250 |
C. elegans |
cat-4(e1141) him-5(e1490) V. Show Description
Catecholamine abnormal. Throws males.
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CF26 |
C. elegans |
lin-22(mu2) IV; him-5(e1490) V. Show Description
Alae-to-ray transformation similar to that of n372 animals.
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CF301 |
C. elegans |
mab-5(e2088) III; unc-31(e169) IV; him-5(e1490) V; muIs9 X. Show Description
muIs9 [hs-mab-5 + C14G10]. Heat-shock inducible mab-5. C14G10 contains a WT copy of unc-31. muIs9 integrated by gamma irradiation.
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