Search Strains

ynIs54 [flp-20p::GFP]. Him.

ynIs57 [flp-2p::GFP].

ynIs64 [flp-17p::GFP].

ynIs66 [flp-7p::GFP].

ynIs67 [flp-6p::GFP].

ynIs72 [flp-1p::GFP].

ynIs78 [flp-8p::GFP].

ynIs80 [flp-21p::GFP].

ynIs87 [flp-21p::GFP].

ynIs97 [flp-1p::GFP].

ynIs59 [flp-18p::GFP].

Temperature-sensitive maternal effect lethal; 99% of embryos fail to hatch at 25 C. Maintain at 15-20 C. Reference: Song et al. (2008) Dev Cell 15:901-12.

Temperature-sensitive maternal effect lethal; ~50% of embryos fail to hatch at 25 C. Maintain at 15-20 C. Reference: Kemp et al. (2007) Genetics 176:95-113.

Unc. bs12 mutation causes sublethal defect in attachment of centrosome to the nucleus in early embryos. Viable 15-25 C. Reference: Kemp et al. (2007) Genetics 176:95-113.

jjIs1092 [(pNUT1) npp-1::GFP + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Non-Unc.

qaIs3502 [YFP::lmn-1 + CFP::H2B + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Non-Unc.

ltIs1[pIC22; pie-1 promoter::knl-3::GFP + unc-119(+)].

ltIs6 [pIC35; pie-1p::kbp-5::GFP-TEV-STag + unc-119(+)].

ltIs7 [(pIC41) pie-1p::kbp-4::GFP::TEV-STag + unc-119(+)]. [NOTE: Array might be prone to silencing; rescue of unc-119 appears incomplete.]

ltIs9 [pie-1p::kbp-3::GFP::TEV-S Tag + unc-119(+)].

ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. qaIs3502[pie-1::YFP::LMN-1 + unc-119(+)].

ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. qaIs3546 [pie-1p::GFP::npp-8 + unc-119(+)]; npp-8 is CeNup155.

ltIs103 [(pAA212) pie-1p::GFPLAP::cav-1 + unc-119(+)].

ltIs104 [(pAA277) pie-1p::GFP::LAP::vps-37 + unc-119(+)].

ltIs105 [(pAA280) pie-1p::GFP::LAP::MVB-12 + unc-119(+)].

ltIs79 [(pAA196) pie-1p::mCherry::rab-5 + unc-119(+)]. pwIs116 [rme-2p::rme-2::GFP::rme-2 3'UTR + unc-119(+)]. Maintain at 20-25C to reduce silencing of the array.

ltIs14 [(pASM05) pie-1p::GFP-TEV-STag::air-2 + unc-119(+)].

Reference: Wang S, et al.  Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094.

Control strain for OD2770

ltIs24 [(pAZ132) pie-1p::GFP::tba-2 + unc-119(+)].

ltIs22[pPM3; pie-1::GFP-TEV-STag::KNL-2 + unc-119(+)].

ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. meIs1 [pie-1p::GFP::lacI]. Made by crossing AV221 with OD56. Reference: Yuen KW, et al. Curr Biol. 2011 Nov 8;21(21):1800-7.

ltIs38 [pie-1p::GFP::PH(PLC1delta1) + unc-119(+)].

ltIs41[pAA5; pie-1::GFP-TEV-STag::CAR-1; unc-119(+)].

ltIs42[pAA19; pie-1::GFP-TEV-Stag::CAR-1deltaN; unc-119(+)].

ltIs43[pAA26; pie-1::GFP-TEV-STag::ZEN-4; unc-119(+)]. Insertion only viable when heterozygous. Pick non-Unc worms to maintain.

ltIs3[pIC31; pie-1 promoter::hcp-1::GFP-TEV-STag + unc-119(+)].

ltIs44 [pie-1p::mCherry::PH(PLC1delta1) + unc-119(+)].

ltIs38 [pie-1p::GFP::PH(PLC1delta1) + unc-119(+)]. ltIs24 [pAZ132; pie-1::GFP::tba-2 + unc-119(+)].

ltIs75 [(pSK5) pie-1::GFP::TEV-STag::LacI + unc-119(+)].

ltIs4 [(pIC32) pie-1p::mis-12::GFP::TEV-STag + unc-119(+)].

ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. qaIs3507 [pie-1::GFP::lem-2 + unc-119(+)].

ltIs5 [(pIC36) pie-1p::kbp-1::GFP::TEV-STag + unc-119(+)].

No obvious phenotype.

No obvious phenotype.

Daf-c. Dyf. Maintain at 15C.

Daf-c. Dyf. Maintain at 15C.

Daf-c. Dyf. Maintain at 15C.

drEx206 [hsf-1p::hsf-1::YFP::unc-54 3'UTR + unc-119(+)]. Reference: Morton EA, Lamitina T. Aging Cell. 2012 Oct 26. doi: 10.1111/acel.12024.

oxIs12 [unc-47p::GFP + lin-15(+)]. Ectopic DVB outgrowth.