Strain Information

Name RG3411   View On Wormbase
Species C. elegans
GenotypeC34G6.3(ve911[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) I.
DescriptionHomozygous viable. Deletion of 480 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: AACGAAGAAGAAGACTTCAAATTTGTCGGT ; Right flanking sequence: GGGCCACCCGCCAGGGCTGTTCAGCAACCG. C34G6.3 sgRNA A: TTCAAGATCCCAAACTTCTG; C34G6.3 sgRNA B: TTGAAGCAAGACATTACACG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.
MutagenCrispr/Cas9
Made byRG KO Group
Laboratory RG
Reference n/a
Sign in or register an account if you want to order this strain.