File: mcat-1_Wildtype.ape

Comments

>C50D2.9.1 unspliced + UTR + 2000 upstream + 2000 downstream; -ive strand; Sequence: 6991 bp
>Deletion size = 1609 bp
>mcat-1 sgRNA #1, Chr II, GGCAAAAGCTTTCCAAAACG; cut=89019; strand=-1; GC=45; 3'GG=no; folding=-2.5
>mcat-1 sgRNA #2, Chr II, TCGAAAACTCGCCGTGCCGC; cut=90623; strand=+1; GC=65; 3'GG=no; folding=-0.9

Features

Name Type Orientation Position
C50D2.6a.1_Unspliced_UTR gene forward 1..1295
mcat-1-1 SV F primer_bind forward 1412..1433
mcat-1 Upstream Homology Arm misc_feature forward 2089..2488
Left Deletion Flank misc_structure forward 2459..2488
mcat-1 sgRNA #1 protein_bind reverse 2489..2508
mcat-1-1 WT F primer_bind forward 3340..3360
mcat-1 sgRNA #2 protein_bind forward 4078..4097
mcat-1 Downstream Homology Arm misc_feature forward 4098..4447
Right Deletion Flank misc_structure forward 4098..4127
mcat-1-1 SV R primer_bind reverse 5216..5237
C50D2.7.1_Unspliced_UTR gene reverse 5641..6991

Sequence

taatgggctccccgtagggcttcataaggcaaaaaggggggggggtgtggttcaaaggcacatacacacaaatgacaataatatttcagaattcttccgatctcttccatgccttgccgagtaagttaccaagtgcatatatcattcacatattacatttcaagttgaaaatgtatagatctcattggtaaatcaattttgatcgacgtcgaagtacgtccaatcgatgcaggtgtccccagctttgacatactttgatgtcgattggactgacttcgacgtcgatcagaatcgatttaacagagtcttgtttaggctaaggggtcaagtgcagcatgaccgaaatttgcttggggcggggggaggggcgtccccataacggccctatttttcccaagcatgaatatttgaatacagatttccatttaaaaaattaatttgaaaaatgtgacgtaataatggtacacatgtaccgactattctgaatcgagttaagtaaactgacaatactttaatataaaaaaagtaatgtagacattttttggtttcctgtagtgtctagttttattagcacgggaatacctgcaccctggctatgcttactttcacgtgaaaaattctaaagttgtcgtacaattagcttcccgctttttttgagcaaagtaagtaagtataccaattgaacgaaaaacgaactgcctaatatcatccaggtctttcagcatttctattttctagacacgaaaagattgccctccaacagtgccgacctcagatcaatgattctctagccgcaagcaatcgattttcggtcacagtactacggaaggagcatcacaacctgagatctcatttcgagatgctctgcaagtaagaaactagccaatccttcaatacaaacttaaatttctctttcagaaaacttggctccatgattgaatgtgtggagcccgtaacacgagcaggctgtggagatcaagcggcaaagatgatgcttcgattcatcactgtcggattttcgagtttcgagcagctctattctcaactgggtatctccgatcaattgcccgcggcttgcaagtcgctgctctcacttcaaaaacgcacggtgctccccgagcgtcgccattatgtcctcccgcaggcttcttcggagtacagtgtcttcagttccacgtcttcgactccgcatattccaataatacttttctcgtatctcatccttttttgaaatctcgcctcatgatttataaataaaacgggtaaactaattatcatgaaacttatattttcaacaattaaaactataataaatacaaacaatgaaaagatcacttcagcaactgaaatgaaaataattcttgtgagcctttttctatgtttgaaaaagcatggcaacgagctaacgtcctgcactgactttgtagaaacggggaaatacttcgttcaaaaactattaaaaagtggcaaaaatggcaaaaaagtggcaaaaaatagcatttttgaaacttctctaaaatggttcattttttagctagaggactcaaaattgatctccaaaccctaaaaaattgccttttttcatggtaatatatggtttcaaacatttttagaaaaattatttttttgcccaaaaaaaaaggacagtcattatgtgatggctgaaaaatcaaaatgggcaaaaataaaaagttgtttatttttgtagggaacgggtaattcatacatgtttaatccaaaaaatctaagtttaacccatcaaaaactattaaaagtggcaaaaaataacatttttgaaacttctctaaaatggttcattttttagctagaggactcaaaattgatctccgaacccttaaaaatggccctttttcaatttaatataatttcaaacatttttagaatttttttgccccccaaaaaaaaggacagatggctgaaaaaagaattgggcaaaaataaaaagttgtttgttgaatgattttagaataaaacgggcactcattatcctgaaatttatattttcaacaattgaaactataataaatataaggctaacataatatataaggctaacataaatataacacaaacaatgaaaaaaatcaatTCAGCAACTGAAATGTGAATAATTCTTGTGAGCCTTTTTCGATGTTTGAAAAAGCATCGCACCGAGCTGACGTCCTGCACCGACTTCGTAGAAGCGGGGGAATGCTTCGTTctgaaattttttaatgtgaaaaatccaaattcttcaccatatttgagtcagcgcgcaagaaaatgtgttttggggcgttgaagttacagtaccacgaaaaatttgaaatttacCTGATGTTTTCTGAACAGCGATTGTTGAATCTGTTCCCATCGAACCGGTGAATTGATTTGTTTGGCGACAGCTCCACGAACGTCTCCCTTCTTCGATGACATCACTTTTCCACTATAATTTGACCAGACATTGCACACCGGACGATGAATCTCCACGTTTTGGAAAGCTTTTGCCACctgaaaacacaaacatgaggaaagcttataaaaacaactttatcggaaaatacaggttctgtttaatatttttctgttaaccagccatgaaccgtacctccggcgcagccccgacttctggggatgagaactattttttcttgaactacagtacttctaccgtaccactatgaaaagttattaatttttgaaaatagcaaagaagcacacacCTGTTCGACGGCAGACTCCATTTGTCGTGTATGAAAAGCTGCCGAAACTGCAAGTCTCTTCATCACTTGAATATTGTATTTCTCTTGATTTTCTTCCAAATATTTTAGGCACGTTTCTGATCCGCCAATTACTCGGACACCGCAATACAGATAGTTGGCAACTTCACATACAACAAGCTCTCGGTTTTCActggaaatttgaacaatcagggtttgggtggccgttcggcaggtttttttgttgaaatatccggcaaatcggtaaattgccggtttgccgtaaattttaaattccggcaatttgccggtttgctggaaattttcggcgattgccgatttgccggaaatgtgcaattccggcaatttgccggagtgccgatttgcgggaaattttcaattcaggaaatttgccgatttagtttattttcaagtaaatttattttttcgcatcaaagttaatttattttcgataaaagtagtttcaaatgttggaaaatagttttctgaaaattttcaatttttatgatttccgtcgggttttttgtactcttcctgcaaaaaccgcaggttttttttgaaaatttttgtgagaaaaaacctacGCCGCCACTTTTCTGGCATCTGCCATTGCCTTATCCAATTTCGATGCAGCTTTTACTCGAATCGTAACCATTCCACTCTTCACCAGTTTCGCGCATTCTGACATTGCTTCGGCTCTGGTTTTCACGATCTTGATAGctggaaaagtctaattttagaagaattgaagaaacacaaaaaccaaattacCATCTGAAAAGCTCAAAACTTTCCCGGCAACAAGTGACGCATATTCTCCGACTGAAAATCCAGCTGTATCAGTCAGGTTTTCTTCGATTGACGGATCTGATGCTTTTAAAGCTTCAAACGCTGCTATTGATGACGTTACGATGGCCGGTTGGCAGTAAAGTGTTTGTTCAAGTctgcaatttttttaaattttttcagacaatattatgaacaggggtgggcggcaaacgatttttccggcaaatcggcaaattgccggaattgaaaaatttcggcaaatcggcaaattgccaaatttgtcgacaaccacattgccgaacggcaattgtcacccactcctgattttgaaaatatgcttggcgattccagaagcattaatagtactgtttgaaaatcgaaaattagataatatcgggcaaaaaacgtatttttgccggtattttttcaccatgagcacaatttgataaagatttctaattttttcagagaataatatttattacTTCTGTTTGGGACCATCTTGACAAACTTTGAGCATGTCATATCCTAGAACTTCGGATGCTTCATCGAAAACTCGCCGTGCCGCTGGAATATCCATCACctgtagaaataaaaaattgaacaattgcgccgaacattattactttaacacCTTTTGACCCATTCCAACAAATTGTGCTCCTTGTCCCGGAAATAATAGAACGACGTGCTCTTCGATGGGTAATTGACTGAAATCAATAGCTCCAGAGTCTTTCTTCTTGCGCGTTCCGTATcttgaaaaatcaaaattaaccgatatatgggctgacaaagcttatggtgcatcgatctcaacgatctgattcttagtactctcaagacggaaaataagtgatggaacgtgattttattcggaaatacagcgaaaacgatcgtagagttgattttctataaaaatttgaatttttgaaaaaaatttttttttgaaattttgaaaacaaaaactccgaacttcagaaattttcaaaaaagaaaaatttaattgcctattttatccgtaccaggctcatcggtatttcgatcttaaattatttttaatgaaaaaaattcaaaaaccaaaaatgtacatttttggccacatttttaataatttccggttatttttcgaaaatttaaaaaagaaaaactattttttgcatgatttttccgagaaaaaaatcaaataataaaattttaaattttttcgaatttccttcatttcaaaattttcaaaaaaaaaccaaaattacCGAATTTTATTCCTTTCCTTCATAATTTCACTTGACGTCTTTTCAATATGCTCACGAAGTTGCTCCTCTGGGTACGGTAGCCGTGTCGTTGGATTAAGAATTACGTTGTGAACATCTGAAAATGTGACTCCTTCATCCAGAATCCCACCTCCTGGAGGTTTTCTTCGAATAAGTCGTGTGGAGCAACGAGCTATGTTAAATGGAGTCAGCATctgaaaaaattgttttttttgaaaattaaaacatttttgttttttcttatatacttgaatacagttgtgacgtaatttttctacactttttaattttccgacactacttgaataaccccataaggtggagttccgaaatctgacactttgcttttttagactaccgaaactagaccaccgagtgccgaaactacccaaaaattttcatactgccgatagactaccgagactaccgattagacggacattttagactaccgaaaaactaccgaaactaataagactttttgaaaatttcacaaaaaagaagttatggcggttcaaagttctggaaaaaatgttgattttcaactggaatcaaaaacttgggtctaaaaaagcaaaatctgagatttcggtactgcactttgaggctttgtaaacattttttttcgattttcaaaaatataccgtttttcaaacttcaaaaatttacttacataaacattaatttcgatccaaaaattataagaattttgaatcatcaaaaagtctaaaaattttcaaaaaaatatttgatttttttctaaaaatcgtcaattttttgatgagactacctgaaatcgcaaaaaaatagaaaaaatggagaaaaccacaaaattcaaaatttagaaaaaaaagggtatttattactatttacatttacaaaaattacaggcacataatcataacaaaattcacagaatctttggggggaaaagagaaaatacatgaaaaaaatggcaaaaaatggggagaatgaacaaatttttacaataaagtacacgtggtgtcagagtgtcccatcacggtgtgatctacaaaaatgcggtgagcgagacgcagaaatcccgcatttcttgtacatcaacccgtgatgggataacccgacaccatgtgaaagtctaccaatgagtgggtctattcaagcgataaaattgtgaataaagaagtccggtagcagaaattgcatcatcaattccgacggtttttgatggtaaacggcatacaagaacaggtgtgaacacgaatagaacatcttcacgcatccatgatgctattgggctggaaacagattgataaggaaaaggaacacaactttttcccccataactacagtaatcctaccgtactcctacagtacccctacattactattacagtaacccgacctacaatcaaagaaaagtgggaaaaattccagattttagcaaaatttggttttttttttgaaattttgacccgaaacgttttgggcaacttttttgtgaagtctcattatgaaattaggtggttttggacaagtttcagtctatttacgcaaaatacccacaaattctactctcagtttaatattgatttttgataacttaaaagcttttttggtgttttttttaattcggtgtttttctctcaattgattcctcttttcacatacttatgagcttcaaattgataattcttctcaatcttcttatccaacacaaaattcgccggagttctaatttccagcagctccgaatccatcgtatttgctccaatattacatgacaatcgaccagcgattctggctccggcagcaaggccagcagcaagattagaccaatctgttccagaagataccataatgtgatacgtgagacaatggaaatgaattcgagagagccggtatccagttttcgacgcaatttgtccagtgggatctcttccatatgtcttcaagagccaatggagcatctctacaactttgtgcacatgaacggttccagcttgtaccggatattcttccatatgtggtccatttgcaatatgtgacaggaatgtgagctcttgctcattaataccaagagaatctacgtatggtaggatcttattgataacatcagttgagaaaatctcatcggccaaacttccaagctccaaatgaatcggaactttcggcgggatttgaagaagatttcgcttgataagcctcatctttt