File: mcat-1_Wildtype.ape

Comments
>C50D2.9.1 unspliced + UTR + 2000 upstream + 2000 downstream; -ive
 strand; Sequence: 6991 bp
>Deletion size = 1609 bp
>mcat-1 sgRNA #1, Chr II, GGCAAAAGCTTTCCAAAACG; cut=89019;
 strand=-1; GC=45; 3'GG=no; folding=-2.5
>mcat-1 sgRNA #2, Chr II, TCGAAAACTCGCCGTGCCGC; cut=90623;
 strand=+1; GC=65; 3'GG=no; folding=-0.9



Features
C50D2.6a.1_Unspliced_UTR	gene	forward	1..1295
 

mcat-1-1 SV F	primer_bind	forward	1412..1433
GCACTGACTTTGTAGAAACGGG

mcat-1 Upstream Homology Arm	misc_feature	forward	2089..2488
CAAACAATGAAAAAAATCAATTCAGCAACTGAAATGTGAATAATTCTTGTGAGCCTTTTTCGATGTTTGAAAAAGCATCGCACCGAGCTGACGTCCTGCACCGACTTCGTAGAAGCGGGGGAATGCTTCGTTCTGAAATTTTTTAATGTGAAAAATCCAAATTCTTCACCATATTTGAGTCAGCGCGCAAGAAAATGTGTTTTGGGGCGTTGAAGTTACAGTACCACGAAAAATTTGAAATTTACCTGATGTTTTCTGAACAGCGATTGTTGAATCTGTTCCCATCGAACCGGTGAATTGATTTGTTTGGCGACAGCTCCACGAACGTCTCCCTTCTTCGATGACATCACTTTTCCACTATAATTTGACCAGACATTGCACACCGGACGATGAATCTCCA

Left Deletion Flank	misc_structure	forward	2459..2488
AGACATTGCACACCGGACGATGAATCTCCA

mcat-1 sgRNA #1	protein_bind	reverse	2489..2508
CGTTTTGGAAAGCTTTTGCC

mcat-1-1 WT F	primer_bind	forward	3340..3360
GCATCTGCCATTGCCTTATCC

mcat-1 sgRNA #2	protein_bind	forward	4078..4097
TCGAAAACTCGCCGTGCCGC

mcat-1 Downstream Homology Arm	misc_feature	forward	4098..4447
TGGAATATCCATCACCTGTAGAAATAAAAAATTGAACAATTGCGCCGAACATTATTACTTTAACACCTTTTGACCCATTCCAACAAATTGTGCTCCTTGTCCCGGAAATAATAGAACGACGTGCTCTTCGATGGGTAATTGACTGAAATCAATAGCTCCAGAGTCTTTCTTCTTGCGCGTTCCGTATCTTGAAAAATCAAAATTAACCGATATATGGGCTGACAAAGCTTATGGTGCATCGATCTCAACGATCTGATTCTTAGTACTCTCAAGACGGAAAATAAGTGATGGAACGTGATTTTATTCGGAAATACAGCGAAAACGATCGTAGAGTTGATTTTCTATAAAAA

Right Deletion Flank	misc_structure	forward	4098..4127
TGGAATATCCATCACCTGTAGAAATAAAAA

mcat-1-1 SV R	primer_bind	reverse	5216..5237
CGAGACTACCGATTAGACGGAC

C50D2.7.1_Unspliced_UTR	gene	reverse	5641..6991
 


formatted sequence

        1 aatgggctcc ccgtagggct tcataaggca aaaagggggg ggggtgtggt tcaaaggcac
       61 atacacacaa atgacaataa tatttcagaa ttcttccgat ctcttccatg ccttgccgag
      121 taagttacca agtgcatata tcattcacat attacatttc aagttgaaaa tgtatagatc
      181 tcattggtaa atcaattttg atcgacgtcg aagtacgtcc aatcgatgca ggtgtcccca
      241 gctttgacat actttgatgt cgattggact gacttcgacg tcgatcagaa tcgatttaac
      301 agagtcttgt ttaggctaag gggtcaagtg cagcatgacc gaaatttgct tggggcgggg
      361 ggaggggcgt ccccataacg gccctatttt tcccaagcat gaatatttga atacagattt
      421 ccatttaaaa aattaatttg aaaaatgtga cgtaataatg gtacacatgt accgactatt
      481 ctgaatcgag ttaagtaaac tgacaatact ttaatataaa aaaagtaatg tagacatttt
      541 ttggtttcct gtagtgtcta gttttattag cacgggaata cctgcaccct ggctatgctt
      601 actttcacgt gaaaaattct aaagttgtcg tacaattagc ttcccgcttt ttttgagcaa
      661 agtaagtaag tataccaatt gaacgaaaaa cgaactgcct aatatcatcc aggtctttca
      721 gcatttctat tttctagaca cgaaaagatt gccctccaac agtgccgacc tcagatcaat
      781 gattctctag ccgcaagcaa tcgattttcg gtcacagtac tacggaagga gcatcacaac
      841 ctgagatctc atttcgagat gctctgcaag taagaaacta gccaatcctt caatacaaac
      901 ttaaatttct ctttcagaaa acttggctcc atgattgaat gtgtggagcc cgtaacacga
      961 gcaggctgtg gagatcaagc ggcaaagatg atgcttcgat tcatcactgt cggattttcg
     1021 agtttcgagc agctctattc tcaactgggt atctccgatc aattgcccgc ggcttgcaag
     1081 tcgctgctct cacttcaaaa acgcacggtg ctccccgagc gtcgccatta tgtcctcccg
     1141 caggcttctt cggagtacag tgtcttcagt tccacgtctt cgactccgca tattccaata
     1201 atacttttct cgtatctcat ccttttttga aatctcgcct catgatttat aaataaaacg
     1261 ggtaaactaa ttatcatgaa acttatattt tcaacaatta aaactataat aaatacaaac
     1321 aatgaaaaga tcacttcagc aactgaaatg aaaataattc ttgtgagcct ttttctatgt
     1381 ttgaaaaagc atggcaacga gctaacgtcc tgcactgact ttgtagaaac ggggaaatac
     1441 ttcgttcaaa aactattaaa aagtggcaaa aatggcaaaa aagtggcaaa aaatagcatt
     1501 tttgaaactt ctctaaaatg gttcattttt tagctagagg actcaaaatt gatctccaaa
     1561 ccctaaaaaa ttgccttttt tcatggtaat atatggtttc aaacattttt agaaaaatta
     1621 tttttttgcc caaaaaaaaa ggacagtcat tatgtgatgg ctgaaaaatc aaaatgggca
     1681 aaaataaaaa gttgtttatt tttgtaggga acgggtaatt catacatgtt taatccaaaa
     1741 aatctaagtt taacccatca aaaactatta aaagtggcaa aaaataacat ttttgaaact
     1801 tctctaaaat ggttcatttt ttagctagag gactcaaaat tgatctccga acccttaaaa
     1861 atggcccttt ttcaatttaa tataatttca aacattttta gaattttttt gccccccaaa
     1921 aaaaaggaca gatggctgaa aaaagaattg ggcaaaaata aaaagttgtt tgttgaatga
     1981 ttttagaata aaacgggcac tcattatcct gaaatttata ttttcaacaa ttgaaactat
     2041 aataaatata aggctaacat aatatataag gctaacataa atataacaca aacaatgaaa
     2101 aaaatcaatT CAGCAACTGA AATGTGAATA ATTCTTGTGA GCCTTTTTCG ATGTTTGAAA
     2161 AAGCATCGCA CCGAGCTGAC GTCCTGCACC GACTTCGTAG AAGCGGGGGA ATGCTTCGTT
     2221 ctgaaatttt ttaatgtgaa aaatccaaat tcttcaccat atttgagtca gcgcgcaaga
     2281 aaatgtgttt tggggcgttg aagttacagt accacgaaaa atttgaaatt tacCTGATGT
     2341 TTTCTGAACA GCGATTGTTG AATCTGTTCC CATCGAACCG GTGAATTGAT TTGTTTGGCG
     2401 ACAGCTCCAC GAACGTCTCC CTTCTTCGAT GACATCACTT TTCCACTATA ATTTGACCAG
     2461 ACATTGCACA CCGGACGATG AATCTCCACG TTTTGGAAAG CTTTTGCCAC ctgaaaacac
     2521 aaacatgagg aaagcttata aaaacaactt tatcggaaaa tacaggttct gtttaatatt
     2581 tttctgttaa ccagccatga accgtacctc cggcgcagcc ccgacttctg gggatgagaa
     2641 ctattttttc ttgaactaca gtacttctac cgtaccacta tgaaaagtta ttaatttttg
     2701 aaaatagcaa agaagcacac acCTGTTCGA CGGCAGACTC CATTTGTCGT GTATGAAAAG
     2761 CTGCCGAAAC TGCAAGTCTC TTCATCACTT GAATATTGTA TTTCTCTTGA TTTTCTTCCA
     2821 AATATTTTAG GCACGTTTCT GATCCGCCAA TTACTCGGAC ACCGCAATAC AGATAGTTGG
     2881 CAACTTCACA TACAACAAGC TCTCGGTTTT CActggaaat ttgaacaatc agggtttggg
     2941 tggccgttcg gcaggttttt ttgttgaaat atccggcaaa tcggtaaatt gccggtttgc
     3001 cgtaaatttt aaattccggc aatttgccgg tttgctggaa attttcggcg attgccgatt
     3061 tgccggaaat gtgcaattcc ggcaatttgc cggagtgccg atttgcggga aattttcaat
     3121 tcaggaaatt tgccgattta gtttattttc aagtaaattt attttttcgc atcaaagtta
     3181 atttattttc gataaaagta gtttcaaatg ttggaaaata gttttctgaa aattttcaat
     3241 ttttatgatt tccgtcgggt tttttgtact cttcctgcaa aaaccgcagg ttttttttga
     3301 aaatttttgt gagaaaaaac ctacGCCGCC ACTTTTCTGG CATCTGCCAT TGCCTTATCC
     3361 AATTTCGATG CAGCTTTTAC TCGAATCGTA ACCATTCCAC TCTTCACCAG TTTCGCGCAT
     3421 TCTGACATTG CTTCGGCTCT GGTTTTCACG ATCTTGATAG ctggaaaagt ctaattttag
     3481 aagaattgaa gaaacacaaa aaccaaatta cCATCTGAAA AGCTCAAAAC TTTCCCGGCA
     3541 ACAAGTGACG CATATTCTCC GACTGAAAAT CCAGCTGTAT CAGTCAGGTT TTCTTCGATT
     3601 GACGGATCTG ATGCTTTTAA AGCTTCAAAC GCTGCTATTG ATGACGTTAC GATGGCCGGT
     3661 TGGCAGTAAA GTGTTTGTTC AAGTctgcaa tttttttaaa ttttttcaga caatattatg
     3721 aacaggggtg ggcggcaaac gatttttccg gcaaatcggc aaattgccgg aattgaaaaa
     3781 tttcggcaaa tcggcaaatt gccaaatttg tcgacaacca cattgccgaa cggcaattgt
     3841 cacccactcc tgattttgaa aatatgcttg gcgattccag aagcattaat agtactgttt
     3901 gaaaatcgaa aattagataa tatcgggcaa aaaacgtatt tttgccggta ttttttcacc
     3961 atgagcacaa tttgataaag atttctaatt ttttcagaga ataatattta ttacTTCTGT
     4021 TTGGGACCAT CTTGACAAAC TTTGAGCATG TCATATCCTA GAACTTCGGA TGCTTCATCG
     4081 AAAACTCGCC GTGCCGCTGG AATATCCATC ACctgtagaa ataaaaaatt gaacaattgc
     4141 gccgaacatt attactttaa cacCTTTTGA CCCATTCCAA CAAATTGTGC TCCTTGTCCC
     4201 GGAAATAATA GAACGACGTG CTCTTCGATG GGTAATTGAC TGAAATCAAT AGCTCCAGAG
     4261 TCTTTCTTCT TGCGCGTTCC GTATcttgaa aaatcaaaat taaccgatat atgggctgac
     4321 aaagcttatg gtgcatcgat ctcaacgatc tgattcttag tactctcaag acggaaaata
     4381 agtgatggaa cgtgatttta ttcggaaata cagcgaaaac gatcgtagag ttgattttct
     4441 ataaaaattt gaatttttga aaaaaatttt tttttgaaat tttgaaaaca aaaactccga
     4501 acttcagaaa ttttcaaaaa agaaaaattt aattgcctat tttatccgta ccaggctcat
     4561 cggtatttcg atcttaaatt atttttaatg aaaaaaattc aaaaaccaaa aatgtacatt
     4621 tttggccaca tttttaataa tttccggtta tttttcgaaa atttaaaaaa gaaaaactat
     4681 tttttgcatg atttttccga gaaaaaaatc aaataataaa attttaaatt ttttcgaatt
     4741 tccttcattt caaaattttc aaaaaaaaac caaaattacC GAATTTTATT CCTTTCCTTC
     4801 ATAATTTCAC TTGACGTCTT TTCAATATGC TCACGAAGTT GCTCCTCTGG GTACGGTAGC
     4861 CGTGTCGTTG GATTAAGAAT TACGTTGTGA ACATCTGAAA ATGTGACTCC TTCATCCAGA
     4921 ATCCCACCTC CTGGAGGTTT TCTTCGAATA AGTCGTGTGG AGCAACGAGC TATGTTAAAT
     4981 GGAGTCAGCA Tctgaaaaaa ttgttttttt tgaaaattaa aacatttttg ttttttctta
     5041 tatacttgaa tacagttgtg acgtaatttt tctacacttt ttaattttcc gacactactt
     5101 gaataacccc ataaggtgga gttccgaaat ctgacacttt gcttttttag actaccgaaa
     5161 ctagaccacc gagtgccgaa actacccaaa aattttcata ctgccgatag actaccgaga
     5221 ctaccgatta gacggacatt ttagactacc gaaaaactac cgaaactaat aagacttttt
     5281 gaaaatttca caaaaaagaa gttatggcgg ttcaaagttc tggaaaaaat gttgattttc
     5341 aactggaatc aaaaacttgg gtctaaaaaa gcaaaatctg agatttcggt actgcacttt
     5401 gaggctttgt aaacattttt tttcgatttt caaaaatata ccgtttttca aacttcaaaa
     5461 atttacttac ataaacatta atttcgatcc aaaaattata agaattttga atcatcaaaa
     5521 agtctaaaaa ttttcaaaaa aatatttgat ttttttctaa aaatcgtcaa ttttttgatg
     5581 agactacctg aaatcgcaaa aaaatagaaa aaatggagaa aaccacaaaa ttcaaaattt
     5641 agaaaaaaaa gggtatttat tactatttac atttacaaaa attacaggca cataatcata
     5701 acaaaattca cagaatcttt ggggggaaaa gagaaaatac atgaaaaaaa tggcaaaaaa
     5761 tggggagaat gaacaaattt ttacaataaa gtacacgtgg tgtcagagtg tcccatcacg
     5821 gtgtgatcta caaaaatgcg gtgagcgaga cgcagaaatc ccgcatttct tgtacatcaa
     5881 cccgtgatgg gataacccga caccatgtga aagtctacca atgagtgggt ctattcaagc
     5941 gataaaattg tgaataaaga agtccggtag cagaaattgc atcatcaatt ccgacggttt
     6001 ttgatggtaa acggcataca agaacaggtg tgaacacgaa tagaacatct tcacgcatcc
     6061 atgatgctat tgggctggaa acagattgat aaggaaaagg aacacaactt tttcccccat
     6121 aactacagta atcctaccgt actcctacag tacccctaca ttactattac agtaacccga
     6181 cctacaatca aagaaaagtg ggaaaaattc cagattttag caaaatttgg tttttttttt
     6241 gaaattttga cccgaaacgt tttgggcaac ttttttgtga agtctcatta tgaaattagg
     6301 tggttttgga caagtttcag tctatttacg caaaataccc acaaattcta ctctcagttt
     6361 aatattgatt tttgataact taaaagcttt tttggtgttt tttttaattc ggtgtttttc
     6421 tctcaattga ttcctctttt cacatactta tgagcttcaa attgataatt cttctcaatc
     6481 ttcttatcca acacaaaatt cgccggagtt ctaatttcca gcagctccga atccatcgta
     6541 tttgctccaa tattacatga caatcgacca gcgattctgg ctccggcagc aaggccagca
     6601 gcaagattag accaatctgt tccagaagat accataatgt gatacgtgag acaatggaaa
     6661 tgaattcgag agagccggta tccagttttc gacgcaattt gtccagtggg atctcttcca
     6721 tatgtcttca agagccaatg gagcatctct acaactttgt gcacatgaac ggttccagct
     6781 tgtaccggat attcttccat atgtggtcca tttgcaatat gtgacaggaa tgtgagctct
     6841 tgctcattaa taccaagaga atctacgtat ggtaggatct tattgataac atcagttgag
     6901 aaaatctcat cggccaaact tccaagctcc aaatgaatcg gaactttcgg cgggatttga
     6961 agaagatttc gcttgataag cctcatcttt t  
//

plain sequence
 
