# File: mcat-1_Wildtype.ape

## Comments

>C50D2.9.1 unspliced + UTR + 2000 upstream + 2000 downstream; -ive strand; Sequence: 6991 bp
>Deletion size = 1609 bp
>mcat-1 sgRNA #1, Chr II, GGCAAAAGCTTTCCAAAACG; cut=89019; strand=-1; GC=45; 3'GG=no; folding=-2.5
>mcat-1 sgRNA #2, Chr II, TCGAAAACTCGCCGTGCCGC; cut=90623; strand=+1; GC=65; 3'GG=no; folding=-0.9

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
C50D2.6a.1_Unspliced_UTR | gene | forward | 1..1295
mcat-1-1 SV F | primer_bind | forward | 1412..1433
mcat-1 Upstream Homology Arm | misc_feature | forward | 2089..2488
Left Deletion Flank | misc_structure | forward | 2459..2488
mcat-1 sgRNA #1 | protein_bind | reverse | 2489..2508
mcat-1-1 WT F | primer_bind | forward | 3340..3360
mcat-1 sgRNA #2 | protein_bind | forward | 4078..4097
mcat-1 Downstream Homology Arm | misc_feature | forward | 4098..4447
Right Deletion Flank | misc_structure | forward | 4098..4127
mcat-1-1 SV R | primer_bind | reverse | 5216..5237
C50D2.7.1_Unspliced_UTR | gene | reverse | 5641..6991

## Sequence

t aattaaaactataataaatacaaacaatgaaaagatcacttcagcaactgaaatgaaaataattcttgtgagcctttttctatgtttgaaaaagcatggcaacgagctaacgtcct gcactgactttgtagaaacggg caaacaatgaaaaaaatcaatTCAGCAACTGAAATGTGAATAATTCTTGTGAGCCTTTTTCGATGTTTGAAAAAGCATCGCACCGAGCTGACGTCCTGCACCGACTTCGTAGAAGCGGGGGAATGCTTCGTTctgaaattttttaatgtgaaaaatccaaattcttcaccatatttgagtcagcgcgcaagaaaatgtgttttggggcgttgaagttacagtaccacgaaaaatttgaaatttacCTGATGTTTTCTGAACAGCGATTGTTGAATCTGTTCCCATCGAACCGGTGAATTGATTTGTTTGGCGACAGCTCCACGAACGTCTCCCTTCTTCGATGACATCACTTTTCCACTATAATTTGACC AGACATTGCACACCGGACGATGAATCTCCA CGTTTTGGAAAGCTTTTGCC GCATCTGCCATTGCCTTATCC TCGAAAACTCGCCGTGCCGC TGGAATATCCATCACctgtagaaataaaaa attgaacaattgcgccgaacattattactttaacacCTTTTGACCCATTCCAACAAATTGTGCTCCTTGTCCCGGAAATAATAGAACGACGTGCTCTTCGATGGGTAATTGACTGAAATCAATAGCTCCAGAGTCTTTCTTCTTGCGCGTTCCGTATcttgaaaaatcaaaattaaccgatatatgggctgacaaagcttatggtgcatcgatctcaacgatctgattcttagtactctcaagacggaaaataagtgatggaacgtgattttattcggaaatacagcgaaaacgatcgtagagttgattttctataaaaa cgagactaccgattagacggac attttagactaccgaaaaactaccgaaactaataagactttttgaaaatttcacaaaaaagaagttatggcggttcaaagttctggaaaaaatgttgattttcaactggaatcaaaaacttgggtctaaaaaagcaaaatctgagatttcggtactgcactttgaggctttgtaaacattttttttcgattttcaaaaatataccgtttttcaaacttcaaaaatttacttacataaacattaatttcgatccaaaaattataagaattttgaatcatcaaaaagtctaaaaattttcaaaaaaatatttgatttttttctaaaaatcgtcaattttttgatgagactacctgaaatcgcaaaaaaatagaaaaaatggagaaaaccacaaaattcaaaattt