# File: mcat-1_Deletion.ape

## Comments

>C50D2.9.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 6991 bp
>gene: C50D2.9.1; chr: II; strain: VH7215; allele: hd7179; deletion size: 1609 bp; type: Heterozygous; QC: PPHP; outcrossed: 0
>Deletion size = 1609 bp
>mcat-1 sgRNA #1, Chr II, GGCAAAAGCTTTCCAAAACG; cut=89019; strand=-1; GC=45; 3'GG=no; folding=-2.5
>mcat-1 sgRNA #2, Chr II, TCGAAAACTCGCCGTGCCGC; cut=90623; strand=+1; GC=65; 3'GG=no; folding=-0.9

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
C50D2.6a.1_Unspliced_UTR | gene | forward | 1..1295
mcat-1-1 SV F | primer_bind | forward | 1412..1433
mcat-1 Upstream Homology Arm | misc_feature | forward | 2089..2488
Left Deletion Flank | misc_structure | forward | 2459..2488
Selection Cassette | variation | forward | 2489..7905
LoxP | misc_recomb | forward | 2510..2543
Pmyo-2 | regulatory:promoter | forward | 2586..3564
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 3091..3114
GFP(S65C) | CDS | forward | 3592..3762,3814..3967,4019..4182,4234..4461
unc-54 3'UTR | 3'UTR | forward | 4598..5332
Prps-27 | regulatory:promoter | forward | 5531..6324
neoR | CDS | forward | 6325..7119
NeoR SEC Insertion Primer 2 | primer_bind | forward | 6626..6647
unc-54 3'UTR | 3'UTR | forward | 7120..7818
LoxP | misc_recomb | forward | 7849..7882
Right Deletion Flank | misc_structure | forward | 7906..7935
mcat-1 Downstream Homology Arm | misc_feature | forward | 7906..8255
mcat-1-1 SV R | primer_bind | reverse | 9024..9045
C50D2.7.1_Unspliced_UTR | gene | reverse | 9449..10799

## Sequence

t aattaaaactataataaatacaaacaatgaaaagatcacttcagcaactgaaatgaaaataattcttgtgagcctttttctatgtttgaaaaagcatggcaacgagctaacgtcct gcactgactttgtagaaacggg caaacaatgaaaaaaatcaatTCAGCAACTGAAATGTGAATAATTCTTGTGAGCCTTTTTCGATGTTTGAAAAAGCATCGCACCGAGCTGACGTCCTGCACCGACTTCGTAGAAGCGGGGGAATGCTTCGTTctgaaattttttaatgtgaaaaatccaaattcttcaccatatttgagtcagcgcgcaagaaaatgtgttttggggcgttgaagttacagtaccacgaaaaatttgaaatttacCTGATGTTTTCTGAACAGCGATTGTTGAATCTGTTCCCATCGAACCGGTGAATTGATTTGTTTGGCGACAGCTCCACGAACGTCTCCCTTCTTCGATGACATCACTTTTCCACTATAATTTGACC AGACATTGCACACCGGACGATGAATCTCCA ccgcggcgcatcaggcggcct ataacttcgtatagcatacattatacgaagttat tacgtagagctcggtacctcgtacgtaatacgactcactagt acaagtttgtacaaaaaagcaggctta ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGG gtaagtttaaacatatatatactaactaaccctgattatttaaattttcag CCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC gtaagtttaaacagttcggtactaactaaccatacatatttaaattttcag GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTT gtaagtttaaacatgattttactaactaactaatctgatttaaattttcag AACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG cattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcag agatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaac ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG ATCTCCTGTCATCTCACCTTGC TCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA ctcgaggtcgacaggcctgcatgcgtcgac ataacttcgtatagcatacattatacgaagttat agttgcaggaccactgcggccgc TGGAATATCCATCACctgtagaaataaaaa attgaacaattgcgccgaacattattactttaacacCTTTTGACCCATTCCAACAAATTGTGCTCCTTGTCCCGGAAATAATAGAACGACGTGCTCTTCGATGGGTAATTGACTGAAATCAATAGCTCCAGAGTCTTTCTTCTTGCGCGTTCCGTATcttgaaaaatcaaaattaaccgatatatgggctgacaaagcttatggtgcatcgatctcaacgatctgattcttagtactctcaagacggaaaataagtgatggaacgtgattttattcggaaatacagcgaaaacgatcgtagagttgattttctataaaaa cgagactaccgattagacggac attttagactaccgaaaaactaccgaaactaataagactttttgaaaatttcacaaaaaagaagttatggcggttcaaagttctggaaaaaatgttgattttcaactggaatcaaaaacttgggtctaaaaaagcaaaatctgagatttcggtactgcactttgaggctttgtaaacattttttttcgattttcaaaaatataccgtttttcaaacttcaaaaatttacttacataaacattaatttcgatccaaaaattataagaattttgaatcatcaaaaagtctaaaaattttcaaaaaaatatttgatttttttctaaaaatcgtcaattttttgatgagactacctgaaatcgcaaaaaaatagaaaaaatggagaaaaccacaaaattcaaaattt