LOCUS       ttr_33_Insertion        9562 bp    DNA        linear       10-JUL-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >C37C3.13.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive
            strand; Sequence: 4817 bp
COMMENT     >gene: C37C3.13.1; chr: V; strain: VH7214; allele: hd7205; deletion
            size: 672 bp; type: Heterozygous; QC: PPHP; outcrossed: 0
COMMENT     >Deletion size = 672 bp
COMMENT     >ttr-33 sgRNA #1, Chr V, CCAATGTTAAAGAAAGTCTT; cut=7837315;
            strand=+1; GC=30; 3'GG=no; folding=-1.1
COMMENT     >ttr-33 sgRNA #2, Chr V, AAACTCTCTTATAGCACAGT; cut=7837967;
            strand=+1; GC=35; 3'GG=no; folding=-0.0
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     variation       2206..7622
                     /locus_tag="Selection Cassette"
                     /label="Selection Cassette"
                     /ApEinfo_label="Selection Cassette"
                     /ApEinfo_fwdcolor="#f0faff"
                     /ApEinfo_revcolor="#f0faff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     regulatory      2303..3281
                     /regulatory_class="promoter"
                     /locus_tag="Pmyo-2"
                     /label="Pmyo-2"
                     /ApEinfo_label="Pmyo-2"
                     /ApEinfo_fwdcolor="#fceeaf"
                     /ApEinfo_revcolor="#fceeaf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(128..2042)
                     /locus_tag="txt-5_Unspliced_UTR"
                     /label="txt-5_Unspliced_UTR"
                     /ApEinfo_label="txt-5_Unspliced_UTR"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(3309..3479,3531..3684,3736..3899,3951..4178)
                     /locus_tag="GFP(S65C)"
                     /label="GFP(S65C)"
                     /ApEinfo_label="GFP(S65C)"
                     /ApEinfo_fwdcolor="#b3dc80"
                     /ApEinfo_revcolor="#b3dc80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1856..2205
                     /locus_tag="ttr-33 Upstream Homology Arm"
                     /label="ttr-33 Upstream Homology Arm"
                     /ApEinfo_label="ttr-33 Upstream Homology Arm"
                     /ApEinfo_fwdcolor="#ff9f23"
                     /ApEinfo_revcolor="#ff9f23"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           6837..7535
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#dbefff"
                     /ApEinfo_revcolor="#dbefff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_structure  2176..2205
                     /locus_tag="Left Deletion Flank"
                     /label="Left Deletion Flank"
                     /ApEinfo_label="Left Deletion Flank"
                     /ApEinfo_fwdcolor="#ff644f"
                     /ApEinfo_revcolor="ff644f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     7566..7599
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#c055fd"
                     /ApEinfo_revcolor="#c055fd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7623..8072
                     /locus_tag="ttr-33 Downstream Homology Arm"
                     /label="ttr-33 Downstream Homology Arm"
                     /ApEinfo_label="ttr-33 Downstream Homology Arm"
                     /ApEinfo_fwdcolor="#ff9f23"
                     /ApEinfo_revcolor="#ff9f23"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     2227..2260
                     /locus_tag="LoxP(1)"
                     /label="LoxP(1)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#c055fd"
                     /ApEinfo_revcolor="#c055fd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  7623..7652
                     /locus_tag="Right Deletion Flank"
                     /label="Right Deletion Flank"
                     /ApEinfo_label="Right Deletion Flank"
                     /ApEinfo_fwdcolor="#ff644f"
                     /ApEinfo_revcolor="ff644f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     regulatory      5248..6041
                     /regulatory_class="promoter"
                     /locus_tag="Prps-27"
                     /label="Prps-27"
                     /ApEinfo_label="Prps-27"
                     /ApEinfo_fwdcolor="#fceeaf"
                     /ApEinfo_revcolor="#fceeaf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2808..2831)
                     /locus_tag="pMYO-2 SEC Insertion Primer 2"
                     /label="pMYO-2 SEC Insertion Primer 2"
                     /ApEinfo_label="pMYO-2 SEC Insertion Primer 2"
                     /ApEinfo_fwdcolor="#fceeaf"
                     /ApEinfo_revcolor="#fceeaf"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           4315..5049
                     /locus_tag="unc-54 3'UTR(1)"
                     /label="unc-54 3'UTR(1)"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#dcf0ff"
                     /ApEinfo_revcolor="#dcf0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             6042..6836
                     /locus_tag="neoR"
                     /label="neoR"
                     /ApEinfo_label="neoR"
                     /ApEinfo_fwdcolor="#b3dc80"
                     /ApEinfo_revcolor="#b3dc80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 1 offset 0"
     primer_bind     6343..6364
                     /locus_tag="NeoR SEC Insertion Primer 2"
                     /label="NeoR SEC Insertion Primer 2"
                     /ApEinfo_label="NeoR SEC Insertion Primer 2"
                     /ApEinfo_fwdcolor="#d5fcaf"
                     /ApEinfo_revcolor="#d5fcaf"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     1358..1378
                     /locus_tag="ttr-33-2 SV F"
                     /label="ttr-33-2 SV F"
                     /ApEinfo_label="ttr-33-2 SV F"
                     /ApEinfo_fwdcolor="#80c7ff"
                     /ApEinfo_revcolor="#46beff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(8662..8683)
                     /locus_tag="ttr-33-2 SV R"
                     /label="ttr-33-2 SV R"
                     /ApEinfo_label="ttr-33-2 SV R"
                     /ApEinfo_fwdcolor="#80c7ff"
                     /ApEinfo_revcolor="#46beff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"

ORIGIN
        1 cgaagaagaa gctgaatatg tcattgttga taccggagct ctacctgaat tatgcatgct
       61 tccagaacaa agagggtctt gttatgataa cattttgaga tggaggtaag tcaaatcaag
      121 aatagaaaat tcgaaaatcc gaaaaacttt ataattatac taaaagcaaa atcttaaaat
      181 ctttcagatt cgactctgaa aagtctcaat gtgtaacctt catgtattct ggatgtaatc
      241 caaatgcaaa tcacttcact agtcaggtta gtttcattat tttgtgtcct ttcgtggaac
      301 tggccccttg gtttctaact tgatcttctc cttccgaata cccaatttga gcaccgctgg
      361 ctcacttttt cgacggtgac gttcctcaat tctagcggcc tctgtatttt ctgagcactc
      421 ttgagcaaca gtttcctcac tggaaatgtt tgtttttcaa gagggagtga gagagagaaa
      481 taaacgtaca atttttgaag ccgcacatga tttgttagaa gtcgatgccg ttctgcagta
      541 tccttcatgt ttcgtagttg tttctgtagt aatttttatg gattaggaac taagaaatca
      601 tcactcactg cggtagttgc atttttgtgc atgcatcttc ccataaaagc aacaaatgca
      661 acaactgata gagccgccac acaaattgca ataattcgaa gtcgatttct aattcctttc
      721 tttacttttt gtctatgctc agctgctttt tcgatgtgct tcttcttgct ggggtcgagc
      781 tcgcaatgag gaaatggttc gatgagtgga ccgtgttttt tgcattgttc acaacggcgt
      841 ccagtgtatt tgtctgggca gtcacacgaa agagtgcggt ttttgaaatc tgaaaatttt
      901 aaatttaaga acaggatcta tagcagtttt gcccatcaca gtcctatgtc tatattaaaa
      961 aaaattatcg gacattaaaa aaaatgtttt ctcatttttt cagtatttct ataaaaactg
     1021 cattcgcatt taatcataac ttttaatcgt taaaaactta gtctttaagt acctggggat
     1081 ccgtaaacac agacaatttc atcacaataa tcgccttcaa atcccacatc acagatgcat
     1141 cttccatttc tcaaaaaccc ctcgacacat ttacttgtat attggcattc acttccaaaa
     1201 tatgagccca cacattcaca tcggtccccc ttccattctc ctttgtttcc gcactgaaat
     1261 aattcaatag attttggaag tttagggcct caaaaatata ccttttccgc tggccgatag
     1321 tcacacattt cacctttcca tccgacttcg caaatgcacg gctcactgaa tagaaggctt
     1381 tccgggtcga agcggaatcc gaccgagagt ccgtgaactg taaattgaaa atttgtaatt
     1441 ccaaaaaaaa aacagctttt gcaaaaatcg tccaaaagaa ttttagagtt agacattatt
     1501 tttctcaaaa agttcaaagt tgtatcagtt ttaaaataaa atatttaata ggattgtaga
     1561 gcttgttaga aaaaataaaa gctacttgaa aaaagaaagg tatccaaaaa ggtattgaga
     1621 tagtttcaag caactctatt tgtaaactgt cgagttttta agttctacaa atctcttata
     1681 acatcgctac atctactatc aaactttgaa aaaaaaccat accacattca aaatgttcac
     1741 atttatctcc agtctgtccc ttgatacaat gacaaatccc tccagcatag attcctccat
     1801 tacgacattc ggctctcgga tcatccagag caacattgtc tagaatactt ctcttttgaa
     1861 gaatacgatg cacgtcgctc aatatatttt catctagatc tagtgagtca tctcgtgatt
     1921 gtgcttttgt tgttgataaa aataggaaga gtaaagtgga aaattgtaaa cagtacatag
     1981 cgttagatac tgacaagtct actatcaatt gatttattta ttgcgtcttg aaaggggtat
     2041 caatgagaga aatagggaga tgggtaaaat gcatttataa gagaatacaa aagatgacgt
     2101 aattgattaa tcagagatca gttgaaaata cttttaagta tcaattaTTA TCTGTGAAGA
     2161 CAGTCACGTG ACTCTGACTC GAACTCAATT TGCATGTTGA TAGTTccgcg gcgcatcagg
     2221 cggcctataa cttcgtatag catacattat acgaagttat tacgtagagc tcggtacctc
     2281 gtacgtaata cgactcacta gtcattttat atctgagtag tatcctttgc tttaaatgtc
     2341 cataaaacta attttataat caataaaaca acgtttgtaa atcaactgag tttacaagta
     2401 gagacattga gggatacttt cactatgcta aagtgataat cgaccaaata ataacttcac
     2461 tttggtattt attcctgtct tataaatgtt atgtatgaat taaattcata tgcatatggc
     2521 tcactctgac aatttttaat aatcttccag atcaatattg actaccgatg cgggtggtct
     2581 tttgctttga attctgctga actttacacc ccgaacagca atgtgtgctt cagcctaaaa
     2641 aaaagtaagt gtgttaatca gtgcccccga ttcttcattt tttgcccctc tctcccgttt
     2701 cgtcggcaaa agaagagaaa ataaagataa gtctcaagat aggttggtaa tcgctaaagt
     2761 ggttgtgtgg ataagagtag caaaatggca ggaagagcac tttgcgcgca cacactgtac
     2821 tcattgttct ggataaaatt ctctcgttgt ttgccgtcgg atgtctgcct ctctgcattg
     2881 agccggcttc ttcactatct ttagttaacc taaaatgccg tttcttttct cgtatcccca
     2941 ctatcccgtt gaggttctct gctctcttcg ctccctaccg ccagcgagca actatccgtg
     3001 ggggcgcctt gctcggaaga tgggggggaa gaaagaagat ttttgctatt tgcacttgag
     3061 aaagagactt ttcctgcgtc gatggttaga gaacagtgtg cagacacttt tcagctacct
     3121 agaattacaa ttggatatcc ccgcctccca atccacccac ccagggaaaa agaagggctc
     3181 gccgaaaatc aaagttatct ccaggctcgc gcatcccacc gagcggttga cttctctcca
     3241 ccacttttca ttttaaccct cgatcgtcag acacagaaat gacaagtttg tacaaaaaag
     3301 caggcttaAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT
     3361 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA
     3421 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg
     3481 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG
     3541 TCACTACTTT CTGTTATGGT GTTCAATGCT TCTCGAGATA CCCAGATCAT ATGAAACGGC
     3601 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA
     3661 AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca
     3721 tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG
     3781 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA
     3841 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg
     3901 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA
     3961 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC
     4021 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC
     4081 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG
     4141 CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATAGca ttcgtagaat tccaactgag
     4201 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc
     4261 agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc
     4321 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     4381 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa acttcttctt aatttctttg
     4441 ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat
     4501 taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta
     4561 tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttttact tctgataaat
     4621 tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc
     4681 agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat
     4741 gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg
     4801 aaattaatgt cctttctttt gctttttggg ggtttcccct attgtttgtc aagagtttcg
     4861 aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg
     4921 gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg
     4981 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca
     5041 taactgttta gatcttcgaa tgcatcgcgc gcaccgtacg tctcgaggaa ttcctgcagg
     5101 atatctggat ccacgaagct tcccatggtg acgtcaccgg ttctagatac ctaggtgagc
     5161 tctggtaccc tctagtcaag gccttaagtg agtcgtatta cggactggcc gtcgttttac
     5221 aggccggatg ggccatggca ccttaacatt tctaaatttt agtttaataa aaaaatttta
     5281 aaataatacg agttttgaga ctaactttca ataaaaacca aaaaattgct tcaaaaaaga
     5341 gctcgcgccg atttacgata aacacagcgc gctacgacac tccgaaaacc gcgccgcacc
     5401 attacattct ccgccccacc gcacggtttg cggaaggcga tgaaattgct gggcctaaaa
     5461 ttttaaccct ttttttcccg tttttcaact gttttcccga ttttttcgat atttttttca
     5521 ggtttttagt tagaaaagta tataaaaatt gcattatttg tagttttcgc tcgtaatttg
     5581 tgatagaaaa cggccaattt gcctagattt gtgattctac caagtggaat ttttgggaaa
     5641 atatcaatat tccacgattt ttattcaaat cataacgttt cgaaattatc agcttaaata
     5701 aaactaaaat aaataataaa atactaaaaa ccctgaattt ctcggttctc ggttcagctt
     5761 gagttcgttc tatacacgtt atcgattttt gtcatttcgt ttaatcatgg gttttgattc
     5821 attttaaata tgaaatttga tttattcctc gtttcgaagt tggtttgtct tgtccggccg
     5881 gtaaacaaat gttattaaaa aaaataataa atttcgaaat ttttcgtaat gttccgcaat
     5941 cgtgtttttc cgtttatctc gtcttccctt ccttcaaata tactatttat tttctcaatt
     6001 cacatatttt cagccagccc tgctcaacaa gtggaataaa aATGATTGAA CAAGATGGAT
     6061 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
     6121 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
     6181 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
     6241 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
     6301 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
     6361 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
     6421 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
     6481 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCAC
     6541 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA
     6601 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA
     6661 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG
     6721 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG
     6781 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAgtcc
     6841 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     6901 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag ccacctctaa
     6961 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     7021 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     7081 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     7141 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     7201 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     7261 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     7321 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     7381 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     7441 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     7501 agaatacatt cccaatatac caaacataac tgtttctcga ggtcgacagg cctgcatgcg
     7561 tcgacataac ttcgtatagc atacattata cgaagttata gttgcaggac cactgcggcc
     7621 gctggttaga aaaagatacg gagaggagaa gtgggaaatc gaattgacca aacaaaagaa
     7681 ctggttttca cttgaaatag aagacgatga aagatataca acagagaaga tcggaagtga
     7741 ttcatctgga gaagaaaatt gagaggagca acttcttgta ttttccactt atttatatac
     7801 ccaatagaat tcacctgatt ctttccgatt tgtgtacatt tcgctgacta acgtgtgctt
     7861 cttcggtttt gtcatttctt attgttcatt gaaaataaac agaacaaagc aatcataagg
     7921 tcgaaaatcc catttagaga tcaagaggtg tacctttaat tgtgcggcat ggcatagttt
     7981 tatcttgctg aactctcacc aattgatgag tatgtcagta gaatggattc catccgatcg
     8041 ttgctccacg gtgatctctt ccgccgcctt ttcatccacc atacccgttg tgtatggctg
     8101 gcaactgtga acagcgcctc agtggaatgt ttagtttgat atacagttta aaataatttt
     8161 ctaaactaaa gaaatcagtt tttgaaacca gtcttgtagg catgtcgggc gcaggcacgc
     8221 taacgtgaaa aatagaattt cgagtggtta actattttat tttcaattaa aatacaatca
     8281 actacacaat gaatgacccg gataaatgaa atacaaatac aagaatttaa aaaaaacatg
     8341 gaaatttaaa cttttccatc atctcccttt gctggaatat tatatttcat tcgataagct
     8401 tccaattcgg cttttctctg atcggatcgt acactgtgtc tctcatccat ctcttgttga
     8461 gctgtcattc tcttctcatt ccatttctga gcttttgctt ttttgtggat tctgttgtat
     8521 tctttgcact tgcagcagca ataacagaag caggcaatga gaattacagc aatgattcca
     8581 gcgcagatgg caataacgat tgcggcggct gatgtgctca cccagcagac attgtatttg
     8641 acatgcttga tattacagtc tggatagtac cagtcgaatg gcatacatct tttcgttttt
     8701 ccaccacacc agaagcaatt ctgaaaaaat gtgtttttga aattttcaat atgtttgctt
     8761 ataaaattga atttaatttt tcaaacagtg tttcagaaac tcaacttctg aaattaggaa
     8821 agtattctca attgagagct gtttttgtat taaaagtttc agtttagaac tacaggtgtg
     8881 aaaaaatctg agcaagtgaa caccaacgta ttgcatcaca gtttacgcgt caatttattc
     8941 gagtgttcat tgtagagaaa gttaggtcac cttccagaaa attaagaaac ttgtttcaga
     9001 catttttgct cttttagagg aatttttttt tagaggaaac acgcaagttt ctttgaaaac
     9061 aaaaacaaaa tatatttttt atccacttac cgagcccttg ccaacacatg tttcacaagt
     9121 gttcaaatcg ttcgatccaa ttctacagta ttcttgtttc tctgaccatg tcatgttatc
     9181 cgcacatact gatactagaa caattgagaa aaagagtagt aatcggtgaa tcatcgttct
     9241 gaaaaatcaa taaatagtaa caacttgagc aagtctcgta actgagcgac aaaaccaaag
     9301 tagtaatgaa atagaaagat agaaaggtaa actcaaaggg ctcgcgtgtg tttgtctatc
     9361 gagtgccaat gagttttagg agtagcgaca gaaataagtt ggcagaagaa gaacatacga
     9421 actatgtcgg gctacaagat tcttgtgttt actttttgaa aaagaaaatg catttgagaa
     9481 aatgcaaatg ttcggcagaa atcgaatgga gtttagagca gaatggtaaa aataaaggtg
     9541 gatcagcaaa aatagttgaa ca   
//
