# File: ttr-33_Wildtype.ape

## Comments

>C37C3.13.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 4817 bp
>Deletion size = 672 bp
>ttr-33 sgRNA #1, Chr V, CCAATGTTAAAGAAAGTCTT; cut=7837315; strand=+1; GC=30; 3'GG=no; folding=-1.1
>ttr-33 sgRNA #2, Chr V, AAACTCTCTTATAGCACAGT; cut=7837967; strand=+1; GC=35; 3'GG=no; folding=-0.0

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
txt-5_Unspliced_UTR | gene | reverse | 128..2042
ttr-33-2 SV F | primer_bind | forward | 1358..1378
ttr-33 Upstream Homology Arm | misc_feature | forward | 1856..2205
Left Deletion Flank | misc_structure | forward | 2176..2205
ttr-33 sgRNA #1 | protein_bind | forward | 2206..2225
ttr-33-2 WT F | primer_bind | forward | 2719..2740
ttr-33 sgRNA #2 | protein_bind | forward | 2858..2877
ttr-33 Downstream Homology Arm | misc_feature | forward | 2878..3327
Right Deletion Flank | misc_structure | forward | 2878..2907
ttr-33-2 SV R | primer_bind | reverse | 3917..3938

## Sequence

cgaagaagaagctgaatatgtcattgttgataccggagctctacctgaattatgcatgcttccagaacaaagagggtcttgttatgataacattttgagatggaggtaagtcaaatcaagaatagaaaattcgaaaatccgaaaaactttataattatactaaaagcaaaatcttaaaatctttcagattcgactctgaaaagtctcaatgtgtaaccttcatgtattctggatgtaatccaaatgcaaatcacttcactagtcaggttagtttcattattttgtgtcctttcgtggaactggccccttggtttctaacttgatcttctccttccgaatacccaatttgagcaccgctggctcactttttcgacggtgacgttcctcaattctagcggcctctgtattttctgagcactcttgagcaacagtttcctcactggaaatgtttgtttttcaagagggagtgagagagagaaataaacgtacaatttttgaagccgcacatgatttgttagaagtcgatgccgttctgcagtatccttcatgtttcgtagttgtttctgtagtaatttttatggattaggaactaagaaatcatcactcactgcggtagttgcatttttgtgcatgcatcttcccataaaagcaacaaatgcaacaactgatagagccgccacacaaattgcaataattcgaagtcgatttctaattcctttctttactttttgtctatgctcagctgctttttcgatgtgcttcttcttgctggggtcgagctcgcaatgaggaaatggttcgatgagtggaccgtgttttttgcattgttcacaacggcgtccagtgtatttgtctgggcagtcacacgaaagagtgcggtttttgaaatctgaaaattttaaatttaagaacaggatctatagcagttttgcccatcacagtcctatgtctatattaaaaaaaattatcggacattaaaaaaaatgttttctcattttttcagtatttctataaaaactgcattcgcatttaatcataacttttaatcgttaaaaacttagtctttaagtacctggggatccgtaaacacagacaatttcatcacaataatcgccttcaaatcccacatcacagatgcatcttccatttctcaaaaacccctcgacacatttacttgtatattggcattcacttccaaaatatgagcccacacattcacatcggtcccccttccattctcctttgtttccgcactgaaataattcaatagattttggaagtttagggcctcaaaaatataccttttccgctggccgatagtcacacatttcacctttccatccgacttcgcaaatgcacggctcactgaatagaaggctttccgggtcgaagcggaatccgaccgagagtccgtgaactgtaaattgaaaatttgtaattccaaaaaaaaaacagcttttgcaaaaatcgtccaaaagaattttagagttagacattatttttctcaaaaagttcaaagttgtatcagttttaaaataaaatatttaataggattgtagagcttgttagaaaaaataaaagctacttgaaaaaagaaaggtatccaaaaaggtattgagatagtttcaagcaactctatttgtaaactgtcgagtttttaagttctacaaatctcttataacatcgctacatctactatcaaactttgaaaaaaaaccataccacattcaaaatgttcacatttatctccagtctgtcccttgatacaatgacaaatccctccagcatagattcctccattacgacattcggctctcggatcatccagagcaacattgtctagaatacttctcttttgaagaatacgatgcacgtcgctcaatatattttcatctagatctagtgagtcatctcgtgattgtgcttttgttgttgataaaaataggaagagtaaagtggaaaattgtaaacagtacatagcgttagatactgacaagtctactatcaattgatttatttattgcgtcttgaaaggggtatcaatgagagaaatagggagatgggtaaaatgcatttataagagaatacaaaagatgacgtaattgattaatcagagatcagttgaaaatacttttaagtatcaattaTTATCTGTGAAGACAGTCACGTGACTCTGACTCGAACTCAATTTGCATGTTGATAGTTCCAATGTTAAAGAAAGTCTTTGGGTTTTCTCCAGATGAAACAAATGATTTTGGAATATTAAACGTGACTCTTCTCTGACAAGGTTTGAGTCCGTCATCACAATCGTGATAGATATTAAGTTTTGGATCAATAGTCATCACTTCGGAAGTGTGTCCGGTAAGAAGGAATTGACCAAGAGAGTCTGTAGTTCCTTCGGCAAGAAGATCGTCAAGATCCGGTCctgaaaaaaacttttattttgaaaaatttcaatgagttgcttcatgttagaatttggaatttttaaagatgttagcaattggtatttaaatgttcaagctaacgtaattagagttattcaaacaagctttatataaaaactttgtgtaagattcggtctaattagaacatcaatttttaacgcagctgataaaaaactttaatttcaagcttcacataattctacttacCGGTATCATCATCGTAGAGCTTCACCTTCGTGTTAGCCAGTGGTTTGTCTCCACACATCAGAACACCCTTAACTCCAGCTGATTGGGTGAATACAGCTTCGGAGCCAATTGCACAAAGTATGAAAAGTGATGAAATGCACGCGAGTCGTGACATtatttttgtctgaaaatacaaacactgactgatctgaccttcatcggagaaactctcttatagcacagttggttagaaaaagatacggagaggagaagtgggaaatcgaattgaccaaacaaaagaactggttttcacttgaaatagaagacgatgaaagatatacaacagagaagatcggaagtgattcatctggagaagaaaattgagaggagcaacttcttgtattttccacttatttatatacccaatagaattcacctgattctttccgatttgtgtacatttcgctgactaacgtgtgcttcttcggttttgtcatttcttattgttcattgaaaataaacagaacaaagcaatcataaggtcgaaaatcccatttagagatcaagaggtgtacctttaattgtgcggcatggcatagttttatcttgctgaactctcaccaattgatgagtatgtcagtagaatggattccatccgatcgttgctccacggtgatctcttccgccgccttttcatccaccatacccgttgtgtatggctggcaactgtgaacagcgcctcagtggaatgtttagtttgatatacagtttaaaataattttctaaactaaagaaatcagtttttgaaaccagtcttgtaggcatgtcgggcgcaggcacgctaacgtgaaaaatagaatttcgagtggttaactattttattttcaattaaaatacaatcaactacacaatgaatgacccggataaatgaaatacaaatacaagaatttaaaaaaaacatggaaatttaaacttttccatcatctccctttgctggaatattatatttcattcgataagcttccaattcggcttttctctgatcggatcgtacactgtgtctctcatccatctcttgttgagctgtcattctcttctcattccatttctgagcttttgcttttttgtggattctgttgtattctttgcacttgcagcagcaataacagaagcaggcaatgagaattacagcaatgattccagcgcagatggcaataacgattgcggcggctgatgtgctcacccagcagacattgtatttgacatgcttgatattacagtctggatagtaccagtcgaatggcatacatcttttcgtttttccaccacaccagaagcaattctgaaaaaatgtgtttttgaaattttcaatatgtttgcttataaaattgaatttaatttttcaaacagtgtttcagaaactcaacttctgaaattaggaaagtattctcaattgagagctgtttttgtattaaaagtttcagtttagaactacaggtgtgaaaaaatctgagcaagtgaacaccaacgtattgcatcacagtttacgcgtcaatttattcgagtgttcattgtagagaaagttaggtcaccttccagaaaattaagaaacttgtttcagacatttttgctcttttagaggaatttttttttagaggaaacacgcaagtttctttgaaaacaaaaacaaaatatattttttatccacttaccgagcccttgccaacacatgtttcacaagtgttcaaatcgttcgatccaattctacagtattcttgtttctctgaccatgtcatgttatccgcacatactgatactagaacaattgagaaaaagagtagtaatcggtgaatcatcgttctgaaaaatcaataaatagtaacaacttgagcaagtctcgtaactgagcgacaaaaccaaagtagtaatgaaatagaaagatagaaaggtaaactcaaagggctcgcgtgtgtttgtctatcgagtgccaatgagttttaggagtagcgacagaaataagttggcagaagaagaacatacgaactatgtcgggctacaagattcttgtgtttactttttgaaaaagaaaatgcatttgagaaaatgcaaatgttcggcagaaatcgaatggagtttagagcagaatggtaaaaataaaggtggatcagcaaaaatagttgaaca