# File: ttr-33_Deletion.ape

## Comments

>C37C3.13.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 4817 bp
>gene: C37C3.13.1; chr: V; strain: VH7214; allele: hd7205; deletion size: 672 bp; type: Heterozygous; QC: PPHP; outcrossed: 0
>Deletion size = 672 bp
>ttr-33 sgRNA #1, Chr V, CCAATGTTAAAGAAAGTCTT; cut=7837315; strand=+1; GC=30; 3'GG=no; folding=-1.1
>ttr-33 sgRNA #2, Chr V, AAACTCTCTTATAGCACAGT; cut=7837967; strand=+1; GC=35; 3'GG=no; folding=-0.0

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
txt-5_Unspliced_UTR | gene | reverse | 128..2042
ttr-33-2 SV F | primer_bind | forward | 1358..1378
ttr-33 Upstream Homology Arm | misc_feature | forward | 1856..2205
Left Deletion Flank | misc_structure | forward | 2176..2205
Selection Cassette | variation | forward | 2206..7622
LoxP | misc_recomb | forward | 2227..2260
Pmyo-2 | regulatory:promoter | forward | 2303..3281
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 2808..2831
GFP(S65C) | CDS | forward | 3309..3479,3531..3684,3736..3899,3951..4178
unc-54 3'UTR | 3'UTR | forward | 4315..5049
Prps-27 | regulatory:promoter | forward | 5248..6041
neoR | CDS | forward | 6042..6836
NeoR SEC Insertion Primer 2 | primer_bind | forward | 6343..6364
unc-54 3'UTR | 3'UTR | forward | 6837..7535
LoxP | misc_recomb | forward | 7566..7599
ttr-33 Downstream Homology Arm | misc_feature | forward | 7623..8072
Right Deletion Flank | misc_structure | forward | 7623..7652
ttr-33-2 SV R | primer_bind | reverse | 8662..8683

## Sequence

cgaagaagaagctgaatatgtcattgttgataccggagctctacctgaattatgcatgcttccagaacaaagagggtcttgttatgataacattttgagatggaggtaagtcaaatcaagaatagaaaattcgaaaatccgaaaaactttataattatactaaaagcaaaatcttaaaatctttcagattcgactctgaaaagtctcaatgtgtaaccttcatgtattctggatgtaatccaaatgcaaatcacttcactagtcaggttagtttcattattttgtgtcctttcgtggaactggccccttggtttctaacttgatcttctccttccgaatacccaatttgagcaccgctggctcactttttcgacggtgacgttcctcaattctagcggcctctgtattttctgagcactcttgagcaacagtttcctcactggaaatgtttgtttttcaagagggagtgagagagagaaataaacgtacaatttttgaagccgcacatgatttgttagaagtcgatgccgttctgcagtatccttcatgtttcgtagttgtttctgtagtaatttttatggattaggaactaagaaatcatcactcactgcggtagttgcatttttgtgcatgcatcttcccataaaagcaacaaatgcaacaactgatagagccgccacacaaattgcaataattcgaagtcgatttctaattcctttctttactttttgtctatgctcagctgctttttcgatgtgcttcttcttgctggggtcgagctcgcaatgaggaaatggttcgatgagtggaccgtgttttttgcattgttcacaacggcgtccagtgtatttgtctgggcagtcacacgaaagagtgcggtttttgaaatctgaaaattttaaatttaagaacaggatctatagcagttttgcccatcacagtcctatgtctatattaaaaaaaattatcggacattaaaaaaaatgttttctcattttttcagtatttctataaaaactgcattcgcatttaatcataacttttaatcgttaaaaacttagtctttaagtacctggggatccgtaaacacagacaatttcatcacaataatcgccttcaaatcccacatcacagatgcatcttccatttctcaaaaacccctcgacacatttacttgtatattggcattcacttccaaaatatgagcccacacattcacatcggtcccccttccattctcctttgtttccgcactgaaataattcaatagattttggaagtttagggcctcaaaaatataccttttccgctggccgatagtcacacatttcacctttccatccgacttcgcaaatgcacggctcactgaatagaaggctttccgggtcgaagcggaatccgaccgagagtccgtgaactgtaaattgaaaatttgtaattccaaaaaaaaaacagcttttgcaaaaatcgtccaaaagaattttagagttagacattatttttctcaaaaagttcaaagttgtatcagttttaaaataaaatatttaataggattgtagagcttgttagaaaaaataaaagctacttgaaaaaagaaaggtatccaaaaaggtattgagatagtttcaagcaactctatttgtaaactgtcgagtttttaagttctacaaatctcttataacatcgctacatctactatcaaactttgaaaaaaaaccataccacattcaaaatgttcacatttatctccagtctgtcccttgatacaatgacaaatccctccagcatagattcctccattacgacattcggctctcggatcatccagagcaacattgtctagaatacttctcttttgaagaatacgatgcacgtcgctcaatatattttcatctagatctagtgagtcatctcgtgattgtgcttttgttgttgataaaaataggaagagtaaagtggaaaattgtaaacagtacatagcgttagatactgacaagtctactatcaattgatttatttattgcgtcttgaaaggggtatcaatgagagaaatagggagatgggtaaaatgcatttataagagaatacaaaagatgacgtaattgattaatcagagatcagttgaaaatacttttaagtatcaattaTTATCTGTGAAGACAGTCACGTGACTCTGACTCGAACTCAATTTGCATGTTGATAGTTccgcggcgcatcaggcggcctataacttcgtatagcatacattatacgaagttattacgtagagctcggtacctcgtacgtaatacgactcactagtcattttatatctgagtagtatcctttgctttaaatgtccataaaactaattttataatcaataaaacaacgtttgtaaatcaactgagtttacaagtagagacattgagggatactttcactatgctaaagtgataatcgaccaaataataacttcactttggtatttattcctgtcttataaatgttatgtatgaattaaattcatatgcatatggctcactctgacaatttttaataatcttccagatcaatattgactaccgatgcgggtggtcttttgctttgaattctgctgaactttacaccccgaacagcaatgtgtgcttcagcctaaaaaaaagtaagtgtgttaatcagtgcccccgattcttcattttttgcccctctctcccgtttcgtcggcaaaagaagagaaaataaagataagtctcaagataggttggtaatcgctaaagtggttgtgtggataagagtagcaaaatggcaggaagagcactttgcgcgcacacactgtactcattgttctggataaaattctctcgttgtttgccgtcggatgtctgcctctctgcattgagccggcttcttcactatctttagttaacctaaaatgccgtttcttttctcgtatccccactatcccgttgaggttctctgctctcttcgctccctaccgccagcgagcaactatccgtgggggcgccttgctcggaagatgggggggaagaaagaagatttttgctatttgcacttgagaaagagacttttcctgcgtcgatggttagagaacagtgtgcagacacttttcagctacctagaattacaattggatatccccgcctcccaatccacccacccagggaaaaagaagggctcgccgaaaatcaaagttatctccaggctcgcgcatcccaccgagcggttgacttctctccaccacttttcattttaaccctcgatcgtcagacacagaaatgacaagtttgtacaaaaaagcaggcttaATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagCCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAGcattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcagcatctcgcgcccgtgcctctgacttctaagtccaattactcttcaacatccctacatgctctttctccctgtgctcccaccccctatttttgttattatcaaaaaaacttcttcttaatttctttgttttttagcttcttttaagtcacctctaacaatgaaattgtgtagattcaaaaatagaattaattcgtaataaaaagtcgaaaaaaattgtgctccctccccccattaataataattctatcccaaaatctacacaatgttctgtgtacacttcttatgttttttttacttctgataaattttttttgaaacatcatagaaaaaaccgcacacaaaataccttatcatatgttacgtttcagtttatgaccgcaatttttatttcttcgcacgtctgggcctctcatgacgtcaaatcatgctcatcgtgaaaaagttttggagtatttttggaatttttcaatcaagtgaaagtttatgaaattaatgtcctttcttttgctttttgggggtttcccctattgtttgtcaagagtttcgaggacggcgtttttcttgctaaaatcacaagtattgatgagcacgatgcaagaaagatcggaagaaggtttgggtttgaggctcagtggaaggtgagtagaagttgataatttgaaagtggagtagtgtctatggggtttttgccttaaatgacagaatacattcccaatataccaaacataactgtttagatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaacatttctaaattttagtttaataaaaaaattttaaaataatacgagttttgagactaactttcaataaaaaccaaaaaattgcttcaaaaaagagctcgcgccgatttacgataaacacagcgcgctacgacactccgaaaaccgcgccgcaccattacattctccgccccaccgcacggtttgcggaaggcgatgaaattgctgggcctaaaattttaaccctttttttcccgtttttcaactgttttcccgattttttcgatatttttttcaggtttttagttagaaaagtatataaaaattgcattatttgtagttttcgctcgtaatttgtgatagaaaacggccaatttgcctagatttgtgattctaccaagtggaatttttgggaaaatatcaatattccacgatttttattcaaatcataacgtttcgaaattatcagcttaaataaaactaaaataaataataaaatactaaaaaccctgaatttctcggttctcggttcagcttgagttcgttctatacacgttatcgatttttgtcatttcgtttaatcatgggttttgattcattttaaatatgaaatttgatttattcctcgtttcgaagttggtttgtcttgtccggccggtaaacaaatgttattaaaaaaaataataaatttcgaaatttttcgtaatgttccgcaatcgtgtttttccgtttatctcgtcttcccttccttcaaatatactatttattttctcaattcacatattttcagccagccctgctcaacaagtggaataaaaATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAgtccaattactcttcaacatccctacatgctctttctccctgtgctcccaccccctatttttgttattatcaaaaaacttctcttaatttctttgttttttagcttcttttaagccacctctaacaatgaaattgtgtagattcaaaaatagaattaattcgtaataaaaagtcgaaaaaaattgtgctccctccccccattaataataattctatcccaaaatctacacaatgttctgtgtacacttcttatgttttttacttctgataaatttttttgaaacatcatagaaaaaaccgcacacaaaataccttatcatatgttacgtttcagtttatgaccgcaatttttatttcttcgcacgtctgggcctctcatgacgtcaaatcatgctcatcgtgaaaaagttttggagtatttttggaatttttcaatcaagtgaaagtttatgaaattaattttcctgcttttgctttttggggtttcccctattgtttgtcaagatttcgaggacggcgtttttcttgctaaaatcacaagtattgatgagcacgatgcaagaaagatcggaagaaggtttgggtttgaggctcagtggaaggtgagtagaagttgataatttgaaagtggagtagtgtctatggggtttttgccttaaatgacagaatacattcccaatataccaaacataactgtttctcgaggtcgacaggcctgcatgcgtcgacataacttcgtatagcatacattatacgaagttatagttgcaggaccactgcggccgctggttagaaaaagatacggagaggagaagtgggaaatcgaattgaccaaacaaaagaactggttttcacttgaaatagaagacgatgaaagatatacaacagagaagatcggaagtgattcatctggagaagaaaattgagaggagcaacttcttgtattttccacttatttatatacccaatagaattcacctgattctttccgatttgtgtacatttcgctgactaacgtgtgcttcttcggttttgtcatttcttattgttcattgaaaataaacagaacaaagcaatcataaggtcgaaaatcccatttagagatcaagaggtgtacctttaattgtgcggcatggcatagttttatcttgctgaactctcaccaattgatgagtatgtcagtagaatggattccatccgatcgttgctccacggtgatctcttccgccgccttttcatccaccatacccgttgtgtatggctggcaactgtgaacagcgcctcagtggaatgtttagtttgatatacagtttaaaataattttctaaactaaagaaatcagtttttgaaaccagtcttgtaggcatgtcgggcgcaggcacgctaacgtgaaaaatagaatttcgagtggttaactattttattttcaattaaaatacaatcaactacacaatgaatgacccggataaatgaaatacaaatacaagaatttaaaaaaaacatggaaatttaaacttttccatcatctccctttgctggaatattatatttcattcgataagcttccaattcggcttttctctgatcggatcgtacactgtgtctctcatccatctcttgttgagctgtcattctcttctcattccatttctgagcttttgcttttttgtggattctgttgtattctttgcacttgcagcagcaataacagaagcaggcaatgagaattacagcaatgattccagcgcagatggcaataacgattgcggcggctgatgtgctcacccagcagacattgtatttgacatgcttgatattacagtctggatagtaccagtcgaatggcatacatcttttcgtttttccaccacaccagaagcaattctgaaaaaatgtgtttttgaaattttcaatatgtttgcttataaaattgaatttaatttttcaaacagtgtttcagaaactcaacttctgaaattaggaaagtattctcaattgagagctgtttttgtattaaaagtttcagtttagaactacaggtgtgaaaaaatctgagcaagtgaacaccaacgtattgcatcacagtttacgcgtcaatttattcgagtgttcattgtagagaaagttaggtcaccttccagaaaattaagaaacttgtttcagacatttttgctcttttagaggaatttttttttagaggaaacacgcaagtttctttgaaaacaaaaacaaaatatattttttatccacttaccgagcccttgccaacacatgtttcacaagtgttcaaatcgttcgatccaattctacagtattcttgtttctctgaccatgtcatgttatccgcacatactgatactagaacaattgagaaaaagagtagtaatcggtgaatcatcgttctgaaaaatcaataaatagtaacaacttgagcaagtctcgtaactgagcgacaaaaccaaagtagtaatgaaatagaaagatagaaaggtaaactcaaagggctcgcgtgtgtttgtctatcgagtgccaatgagttttaggagtagcgacagaaataagttggcagaagaagaacatacgaactatgtcgggctacaagattcttgtgtttactttttgaaaaagaaaatgcatttgagaaaatgcaaatgttcggcagaaatcgaatggagtttagagcagaatggtaaaaataaaggtggatcagcaaaaatagttgaaca