LOCUS       ttr_33_Wildtype         4817 bp    DNA        linear       10-JUL-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >C37C3.13.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive
            strand; Sequence: 4817 bp
COMMENT     >Deletion size = 672 bp
COMMENT     >ttr-33 sgRNA #1, Chr V, CCAATGTTAAAGAAAGTCTT; cut=7837315;
            strand=+1; GC=30; 3'GG=no; folding=-1.1
COMMENT     >ttr-33 sgRNA #2, Chr V, AAACTCTCTTATAGCACAGT; cut=7837967;
            strand=+1; GC=35; 3'GG=no; folding=-0.0
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     gene            complement(128..2042)
                     /locus_tag="txt-5_Unspliced_UTR"
                     /label="txt-5_Unspliced_UTR"
                     /ApEinfo_label="txt-5_Unspliced_UTR"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1856..2205
                     /locus_tag="ttr-33 Upstream Homology Arm"
                     /label="ttr-33 Upstream Homology Arm"
                     /ApEinfo_label="ttr-33 Upstream Homology Arm"
                     /ApEinfo_fwdcolor="#ff9f23"
                     /ApEinfo_revcolor="#ff9f23"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    2206..2225
                     /bound_moiety=""
                     /locus_tag="ttr-33 sgRNA #1"
                     /label="ttr-33 sgRNA #1"
                     /ApEinfo_label="ttr-33 sgRNA #1"
                     /ApEinfo_fwdcolor="#fd81ff"
                     /ApEinfo_revcolor="#c2acff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_structure  2176..2205
                     /locus_tag="Left Deletion Flank"
                     /label="Left Deletion Flank"
                     /ApEinfo_label="Left Deletion Flank"
                     /ApEinfo_fwdcolor="#ff644f"
                     /ApEinfo_revcolor="ff644f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2878..3327
                     /locus_tag="ttr-33 Downstream Homology Arm"
                     /label="ttr-33 Downstream Homology Arm"
                     /ApEinfo_label="ttr-33 Downstream Homology Arm"
                     /ApEinfo_fwdcolor="#ff9f23"
                     /ApEinfo_revcolor="#ff9f23"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    2858..2877
                     /bound_moiety=""
                     /locus_tag="ttr-33 sgRNA #2"
                     /label="ttr-33 sgRNA #2"
                     /ApEinfo_label="ttr-33 sgRNA #2"
                     /ApEinfo_fwdcolor="#fd81ff"
                     /ApEinfo_revcolor="#c2acff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_structure  2878..2907
                     /locus_tag="Right Deletion Flank"
                     /label="Right Deletion Flank"
                     /ApEinfo_label="Right Deletion Flank"
                     /ApEinfo_fwdcolor="#ff644f"
                     /ApEinfo_revcolor="ff644f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     1358..1378
                     /locus_tag="ttr-33-2 SV F"
                     /label="ttr-33-2 SV F"
                     /ApEinfo_label="ttr-33-2 SV F"
                     /ApEinfo_fwdcolor="#80c7ff"
                     /ApEinfo_revcolor="#46beff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3917..3938)
                     /locus_tag="ttr-33-2 SV R"
                     /label="ttr-33-2 SV R"
                     /ApEinfo_label="ttr-33-2 SV R"
                     /ApEinfo_fwdcolor="#80c7ff"
                     /ApEinfo_revcolor="#46beff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     2719..2740
                     /locus_tag="ttr-33-2 WT F"
                     /label="ttr-33-2 WT F"
                     /ApEinfo_label="ttr-33-2 WT F"
                     /ApEinfo_fwdcolor="#80c7ff"
                     /ApEinfo_revcolor="#46beff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"

ORIGIN
        1 cgaagaagaa gctgaatatg tcattgttga taccggagct ctacctgaat tatgcatgct
       61 tccagaacaa agagggtctt gttatgataa cattttgaga tggaggtaag tcaaatcaag
      121 aatagaaaat tcgaaaatcc gaaaaacttt ataattatac taaaagcaaa atcttaaaat
      181 ctttcagatt cgactctgaa aagtctcaat gtgtaacctt catgtattct ggatgtaatc
      241 caaatgcaaa tcacttcact agtcaggtta gtttcattat tttgtgtcct ttcgtggaac
      301 tggccccttg gtttctaact tgatcttctc cttccgaata cccaatttga gcaccgctgg
      361 ctcacttttt cgacggtgac gttcctcaat tctagcggcc tctgtatttt ctgagcactc
      421 ttgagcaaca gtttcctcac tggaaatgtt tgtttttcaa gagggagtga gagagagaaa
      481 taaacgtaca atttttgaag ccgcacatga tttgttagaa gtcgatgccg ttctgcagta
      541 tccttcatgt ttcgtagttg tttctgtagt aatttttatg gattaggaac taagaaatca
      601 tcactcactg cggtagttgc atttttgtgc atgcatcttc ccataaaagc aacaaatgca
      661 acaactgata gagccgccac acaaattgca ataattcgaa gtcgatttct aattcctttc
      721 tttacttttt gtctatgctc agctgctttt tcgatgtgct tcttcttgct ggggtcgagc
      781 tcgcaatgag gaaatggttc gatgagtgga ccgtgttttt tgcattgttc acaacggcgt
      841 ccagtgtatt tgtctgggca gtcacacgaa agagtgcggt ttttgaaatc tgaaaatttt
      901 aaatttaaga acaggatcta tagcagtttt gcccatcaca gtcctatgtc tatattaaaa
      961 aaaattatcg gacattaaaa aaaatgtttt ctcatttttt cagtatttct ataaaaactg
     1021 cattcgcatt taatcataac ttttaatcgt taaaaactta gtctttaagt acctggggat
     1081 ccgtaaacac agacaatttc atcacaataa tcgccttcaa atcccacatc acagatgcat
     1141 cttccatttc tcaaaaaccc ctcgacacat ttacttgtat attggcattc acttccaaaa
     1201 tatgagccca cacattcaca tcggtccccc ttccattctc ctttgtttcc gcactgaaat
     1261 aattcaatag attttggaag tttagggcct caaaaatata ccttttccgc tggccgatag
     1321 tcacacattt cacctttcca tccgacttcg caaatgcacg gctcactgaa tagaaggctt
     1381 tccgggtcga agcggaatcc gaccgagagt ccgtgaactg taaattgaaa atttgtaatt
     1441 ccaaaaaaaa aacagctttt gcaaaaatcg tccaaaagaa ttttagagtt agacattatt
     1501 tttctcaaaa agttcaaagt tgtatcagtt ttaaaataaa atatttaata ggattgtaga
     1561 gcttgttaga aaaaataaaa gctacttgaa aaaagaaagg tatccaaaaa ggtattgaga
     1621 tagtttcaag caactctatt tgtaaactgt cgagttttta agttctacaa atctcttata
     1681 acatcgctac atctactatc aaactttgaa aaaaaaccat accacattca aaatgttcac
     1741 atttatctcc agtctgtccc ttgatacaat gacaaatccc tccagcatag attcctccat
     1801 tacgacattc ggctctcgga tcatccagag caacattgtc tagaatactt ctcttttgaa
     1861 gaatacgatg cacgtcgctc aatatatttt catctagatc tagtgagtca tctcgtgatt
     1921 gtgcttttgt tgttgataaa aataggaaga gtaaagtgga aaattgtaaa cagtacatag
     1981 cgttagatac tgacaagtct actatcaatt gatttattta ttgcgtcttg aaaggggtat
     2041 caatgagaga aatagggaga tgggtaaaat gcatttataa gagaatacaa aagatgacgt
     2101 aattgattaa tcagagatca gttgaaaata cttttaagta tcaattaTTA TCTGTGAAGA
     2161 CAGTCACGTG ACTCTGACTC GAACTCAATT TGCATGTTGA TAGTTCCAAT GTTAAAGAAA
     2221 GTCTTTGGGT TTTCTCCAGA TGAAACAAAT GATTTTGGAA TATTAAACGT GACTCTTCTC
     2281 TGACAAGGTT TGAGTCCGTC ATCACAATCG TGATAGATAT TAAGTTTTGG ATCAATAGTC
     2341 ATCACTTCGG AAGTGTGTCC GGTAAGAAGG AATTGACCAA GAGAGTCTGT AGTTCCTTCG
     2401 GCAAGAAGAT CGTCAAGATC CGGTCctgaa aaaaactttt attttgaaaa atttcaatga
     2461 gttgcttcat gttagaattt ggaattttta aagatgttag caattggtat ttaaatgttc
     2521 aagctaacgt aattagagtt attcaaacaa gctttatata aaaactttgt gtaagattcg
     2581 gtctaattag aacatcaatt tttaacgcag ctgataaaaa actttaattt caagcttcac
     2641 ataattctac ttacCGGTAT CATCATCGTA GAGCTTCACC TTCGTGTTAG CCAGTGGTTT
     2701 GTCTCCACAC ATCAGAACAC CCTTAACTCC AGCTGATTGG GTGAATACAG CTTCGGAGCC
     2761 AATTGCACAA AGTATGAAAA GTGATGAAAT GCACGCGAGT CGTGACATta tttttgtctg
     2821 aaaatacaaa cactgactga tctgaccttc atcggagaaa ctctcttata gcacagttgg
     2881 ttagaaaaag atacggagag gagaagtggg aaatcgaatt gaccaaacaa aagaactggt
     2941 tttcacttga aatagaagac gatgaaagat atacaacaga gaagatcgga agtgattcat
     3001 ctggagaaga aaattgagag gagcaacttc ttgtattttc cacttattta tatacccaat
     3061 agaattcacc tgattctttc cgatttgtgt acatttcgct gactaacgtg tgcttcttcg
     3121 gttttgtcat ttcttattgt tcattgaaaa taaacagaac aaagcaatca taaggtcgaa
     3181 aatcccattt agagatcaag aggtgtacct ttaattgtgc ggcatggcat agttttatct
     3241 tgctgaactc tcaccaattg atgagtatgt cagtagaatg gattccatcc gatcgttgct
     3301 ccacggtgat ctcttccgcc gccttttcat ccaccatacc cgttgtgtat ggctggcaac
     3361 tgtgaacagc gcctcagtgg aatgtttagt ttgatataca gtttaaaata attttctaaa
     3421 ctaaagaaat cagtttttga aaccagtctt gtaggcatgt cgggcgcagg cacgctaacg
     3481 tgaaaaatag aatttcgagt ggttaactat tttattttca attaaaatac aatcaactac
     3541 acaatgaatg acccggataa atgaaataca aatacaagaa tttaaaaaaa acatggaaat
     3601 ttaaactttt ccatcatctc cctttgctgg aatattatat ttcattcgat aagcttccaa
     3661 ttcggctttt ctctgatcgg atcgtacact gtgtctctca tccatctctt gttgagctgt
     3721 cattctcttc tcattccatt tctgagcttt tgcttttttg tggattctgt tgtattcttt
     3781 gcacttgcag cagcaataac agaagcaggc aatgagaatt acagcaatga ttccagcgca
     3841 gatggcaata acgattgcgg cggctgatgt gctcacccag cagacattgt atttgacatg
     3901 cttgatatta cagtctggat agtaccagtc gaatggcata catcttttcg tttttccacc
     3961 acaccagaag caattctgaa aaaatgtgtt tttgaaattt tcaatatgtt tgcttataaa
     4021 attgaattta atttttcaaa cagtgtttca gaaactcaac ttctgaaatt aggaaagtat
     4081 tctcaattga gagctgtttt tgtattaaaa gtttcagttt agaactacag gtgtgaaaaa
     4141 atctgagcaa gtgaacacca acgtattgca tcacagttta cgcgtcaatt tattcgagtg
     4201 ttcattgtag agaaagttag gtcaccttcc agaaaattaa gaaacttgtt tcagacattt
     4261 ttgctctttt agaggaattt ttttttagag gaaacacgca agtttctttg aaaacaaaaa
     4321 caaaatatat tttttatcca cttaccgagc ccttgccaac acatgtttca caagtgttca
     4381 aatcgttcga tccaattcta cagtattctt gtttctctga ccatgtcatg ttatccgcac
     4441 atactgatac tagaacaatt gagaaaaaga gtagtaatcg gtgaatcatc gttctgaaaa
     4501 atcaataaat agtaacaact tgagcaagtc tcgtaactga gcgacaaaac caaagtagta
     4561 atgaaataga aagatagaaa ggtaaactca aagggctcgc gtgtgtttgt ctatcgagtg
     4621 ccaatgagtt ttaggagtag cgacagaaat aagttggcag aagaagaaca tacgaactat
     4681 gtcgggctac aagattcttg tgtttacttt ttgaaaaaga aaatgcattt gagaaaatgc
     4741 aaatgttcgg cagaaatcga atggagttta gagcagaatg gtaaaaataa aggtggatca
     4801 gcaaaaatag ttgaaca    
//
