File: mrpl-39_Wildtype.ape

Comments

>Y46H3A.7a.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 5517 bp
>Deletion size = 670 bp
>mrpl-39 sgRNA #1, Chr V, ATTATTTTCAGACGCTGTCC; cut=1822064; strand=+1; GC=40; 3'GG=no; folding=-1.3
>mrpl-39 sgRNA #2, Chr V, CCGCGAAGAGAAGCAATTCG; cut=1822714; strand=+1; GC=55; 3'GG=no; folding=-3.7

Features

Name Type Orientation Position
mrpl-39 SV F primer_bind forward 1652..1672
mrpl-39 Upstream Homolgy Arm misc_feature forward 2019..2468
Left Deletion Flank misc_structure forward 2439..2468
mrpl-39 sgRNA #1 protein_bind forward 2469..2488
mrpl-39 WT F primer_bind forward 2883..2904
mrpl-39 sgRNA #2 protein_bind forward 3119..3138
Right Deletion Flank misc_structure forward 3139..3168
mrpl-39 Downstream Homolgy Arm misc_feature forward 3139..3488
mrpl-39 SV R primer_bind reverse 3613..3634

Sequence

gtcactaaatttcgagtaaaattacacgaaaaacatgaaaaatcagtcaaaaatgagaaaaattgatgaataaaaattaaaatgatgcaagcgcgctccaacgaacgagttcaattggcggtaattcaaatgggaatttgatgcaattttcaaaaaatcatataaaatctagaaaatttttttgaattttttaaatcatgatatttggtctttgtgaccccataggcatgttttaaagcaatttccccacagagcagagtccagaaaaattttagcaaaaaactgccgcaactggagaaaattctcgtaaatctttttttttaactagaaattcaaagtcaggggtcctcctgcaaacttacgcttgtcagaatcatcgtcaaccatcacaacctgctcgatcaactctggcggggatctgagcagcaccgaatgcacagttcggagcaacggagtccatccctcattgtggaacacaacaaccactgaaacagtcggcagcttttctggataatcccaatgcttgcactcttccggcctgatatccggaatagttcgattcatcgagatcatatcggagacgtaggtattgaagccgaactccttctccgctgctcttcctgccgccattccttcttcatcggttactggcacaggttttccgtgctctccgggctgatttgatgggatttccggctcttttggctcgtagtttcctagttctgagccacggaagattgtattaggatcaggaccaccggggagctccttatttgtgagatcgtctaaatcatcaaattgttgagcggcactgtccgatttgatagatgacatgctctgaaattcattttgtttttgagttttgtttctattccctgaaatgtggactacgctcagtgggaaaattgctttaaaacacgcctatggggtcacaataaccgaatatcatagtaaaaaaattcaaaaaaattttctagattttatatgattttttgaaaattggaaaaatctcagttttccacttaattcctatttgaatttccgccattatttgtattaatttattttattttttctcattatttcactgattttctttattatttgtgtatttttatcggaaaaagagacagaaatgcatattttcattaaaaatcactgaaaatgggtaaaactgaacctgaaattatcatatttcacagttttacgcatttacagtgacttttaatgaaaaatttgcatgtatcttgcatttttctcactttttccaataaaaatacacaaataatgaagaaaatcagtgaaataatgataaaaaatgaaattaattaattaaaataatgcaagcacgctccatagaacgaatccaattggcggaaattcaaataggaattattagggggaaaattgagatttttccaattttcaaaaaatcatataaaatttagagactttttttgaattttttttggtcattgtgaccccagaggcatgttttacagcaatttccccattaggcgcactccaaaaaaacctacatttcccttgctattgtagacaaatccaaataaaactaaaactcccaggcaaatatacgtagccactcggcatcctctgaaacaaaatttattaattttaaagtagagaaaaacaaaacctcacctttgcctccataatctctgtagttgcagctttttccgggcgattatcatgtcagatccggttggtttaatcaagttttcgttctgaaacgcaaaaaatacgttaaattgacttaaaaattgaacatttcggtcaaaaaagcacaaattcggtagaaaaatcgtagaaaaatcgtcaatgtgttggcaaatacccgactccgcccacatgcaaggcgcggcacgcgttttcgcaatctgcgcacacactttggtttccggtgattttatttttatttttctgatttctttttttaatggttttttgctttaaatcacttaaaaaaacatatttttcaggtcaataaaacATGAAACTACCTGTCAGAAGCCTGCTGGCAATCCGGAGCGCATCAACTGCTGTGGCATCGGCCGAAAgtatgagaaaagttattcaggctttttttctagcttctatagaaataatttttaattattaaatgtataaaattgttgagaagcatcctacacatcgaaaatgtatctgaaccgccagttttgcgatttctgtttcgtaaaacatgtaattttgtgaggaaaagctgtttcttgacagaaatcgttgattttacccactttctgatcgtttgatgcttttaaatgatatttttcgtttgaatttgcgaaaaaattgataaaaagctgaaaaagagaaaatttaaacggtgttcaataattatggaggctgtgagttgcattaaataccccaaatttcttgaaaataatatttataacaaagaaaaaaacgataaaaaatgcttataaaatattattttcagACGCTGTCCCGGCTTCCGTTCCAGCAGCGACTCTTTCCTCCGAGTTCTTCGATCACATTCGCCATCAAGTGTACGGGGACTTGAAAAAAGTGGACAAAGTCGTGGTTTCAGTGAGAACTGAGGATAAGGCTCAGCCAGTTCTTGTGAATCGGAATGTGTCCACATCATTTCACTGTTTTAATCgtaagaaagcagattttatgaataaataattaattttccccaatttcagATATCTCCAAGCACTTCGCCGACGACGCCGTACTCGTGGAAGTTCATCCATCCGTTGGCGGCGCCTACTTCAGCAGCGTCAATCAACCACTTCAAGATCAAGCCGAGATTCGAAAAATTGGATTCGATTCTGCTGAGGACGTGAATTTAGTGAATGATGCCTACTGGAGATCTTGTTCGCTAGTTACTGCTGCATTCCTGAAAGAAGCTCTGGACGTGGACGTGGATTTCAGATTCCCACGTGGAAATATTGAAAATGGATATTTTTCGGTGGATGTGAAGGGATTGAATGGGAACGTTTTCACCTTGgtgagcagtttttttttttgaaataaaattaattaaaatttttaaaattgattttcagGACGAACTTAACACGATAAATCGATTCGGTAAATCGTACATCCGCGAAGAGAAGCAATTCGAGGTGATCGCAGTGCCAACGAGTGTAGCAGAAGCATCAGGAATCCACGGCGATCATCTAGTACGAATCGGTCGCCAAGTGTTCTCCACAGATGGCCCATGCATCAATTCGACTCGTCAAATCGGCCGCTTCACGATTTTCCGATCAAAATCATCTGGCAAGAGTGTGATCGTCGGCGGAGTGTCGATTCCCATGAAACAGCCGACTTCATCATACTCCTGGAGCCTCGTCGCCAAGAATGCAGTGCAGAAATTTAGTAAAAATGCATGAgaattgaaattcaatagttttttatagctagtttctgattgttgtttttcttttttgttacttttttctgttcattaaaagaacaaaaattataaagctctaaataatttattaagtatgtacaaaaacaaaaaaataatctaacacagtgccctttcagagactaattatccattcttcaagaaattattcaaaaatccctgcgccacctccccggtaatcaaattgttcagctgtcccaacacagctgcaggcgactgatccatcattccagacacttcttcgcatctcttcatggctctctgcaattgtgttccgatttccaagcttgccatcacatcacacgtggcaacggcttctcgcagcgaatcacactgttgccgttctgcaccacgtcggcacgtctcctgggatgctttcagcaaatcgatttctccgtgaagaattgctcgtcgaagttcttcaccccacgcgtcgaacgtcttctcgtcggcttgatcctggcattttccatacgcgatttgcatgaattccgatataaatggaaggcagcctttgccacgtggcaggccaaagttactatccgtatttggcataaaatctcggattccgatctgcgggagtggctccgctttgcctccctcagttggggtcccggtgccggtgccaacttgaaggaatcgacgggcgatttgcatgaatgggctgccgtcgaaagcgtttgtctggaaatattttatttttatttttaaatttgccgctaggcgatttcgaatttattatgcggacccaatcgcgctccacggctaatcgaaagcgctccgcccctaaacgttgggtctcgttaggtatttggtggaaaaactgtaaattcaaacgctttcggcaatttttaggatttttttgcaaaaccacaaaatttcgactatcgttgttttttcggcaatttgccggtttaccggaaatttcaattccggcaatgtgccggtttgccaattttccggaacatttcaattccggcaatttgccgatttgccgtaacatttcaattccggcaatttatcgattttccggaaatttcaattccgccaaattgccgatttgccggtaatttcaattccggcaatttgctgatttgccaatttgccggaaatatcaattccgccaatttgccgatttgccggaactatcgattccgccaatttgccgatttgccggtaatttcaatttttgcaatttgccggtttgccgatttgccggaacatttaaattccgccattttgccaatttgccgttatgcctatttgccggaaaaaagaaatgctcgtaatttggcgaaacgaattgaaaacggttgaatttacgattttaccgccaaatacctaatgagacccaacgatggtaggcggagcgttttcaattggagcgcgttttgggcctcacacgtggagtcagaatgtctcatttcgctttgatcttcaaaaaatgtgggagttgagacgcagacatctcatctgattttgcatggttaagagcgtgctgacgtcacaattttctggagaaatattaccgcatttttcgtagatcaaaccgcaatgagacagcccgacaccatgtggggccttataataacaaaataaatcataccggcgctggtggtcttgctagttcgggagcactcacagctgcagcagaagaagacgcggaagccggtgcggcaatttccacgggagccggaatattagctggagcagcttctacagccacctcggctatcggctcggcatatcgaatctcccgaatcttctctcccatcgccacgtttttcttgaaatcccgcacacccaacacatcacgaccgcccatgtcgaaagtattatcattcgactcttgttgcttcggaatcccaaacattggtggaagtgttgctggtggtggtggagtcgatggggttccgaatgttaatggtgggagggtcggaaatgtggatggtggtggtgttggtggcgtagaagctccggaagcttccagcttatccagggcttgtaggaaagcatttcggccttggaatgcgagattttgaattgctgaaaattacggaaatagttta