File: K07F5.15_Wildtype.ape

Comments

>K07F5.15.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 5063 bp
>Deletion size = 541 bp
>K07F5.15 sgRNA #1, Chr IV, CAAAAGACAGCCTTGCCAAA; cut=9865831; strand=+1; GC=45; 3'GG=no; folding=-0.5
>K07F5.15 sgRNA #2, Chr IV, AAAAGTGATTTCGTAGGCGG; cut=9866337; strand=-1; GC=45; 3'GG=yes; folding=-0.8

Features

Name Type Orientation Position
K07F5.22_Unspliced_UTR gene forward 2001..2857
K07F5.15-1 SV F primer_bind forward 2098..2119
K07F5.15 Upstream Homology Arm misc_feature forward 2387..2736
Left Deletion Flank misc_structure forward 2707..2736
K07F5.15 sgRNA #1 protein_bind forward 2737..2756
K07F5.15-1 WT F primer_bind forward 3087..3108
K07F5.15 sgRNA #2 protein_bind reverse 3258..3277
K07F5.15 Downstream Homology Arm misc_feature forward 3278..3727
Right Deletion Flank misc_structure forward 3278..3307
K07F5.15-1 SV R primer_bind reverse 4037..4059

Sequence

ggaatggaagtgacgaaggagacgaagattcaactgaaaagaaaaaggaagaagtcgttgaaaaagacgaggaaagcggtgatgagagctttgagaaagctgaaatctttttcaaaatggatgacgcgttttcagctgaagtgagttttcaacaaaaaaaaacatttttcacacaaaaattattttcagatgaaagaacgaaaagatggtgatgatccgtcaactggaggtttctcttttttatcaatgattggaagaaaatatgatgacgaagcagttgaagatgatggagaaaatttaaagaaaatagaaccaacacaaagtaaagccgaaccgtctggaaatgttgacaaaatcgaaaaatctctactcaaagtgaaagttagcacaaagtttttcgtcgatcctctgtcggatgaaaaaatcaaaagtttagcggctaatttcagaagaactcaaacagtggagaaagttattgataaatgggcacctcatcgagatgctattttcaaggtgagtagttttttttttgagcctgaccgactattgagctctgttttattatttcaagcgagtcttaatacaaaaaagatttcaaattgttttgattcagaaagttataccaatttccagtatgatcaaataagtccaccatcttaataaataacaaaattttccctaactgtctgattctcgttacgcatacacaatctcattctcaatctttttgcggaaaagtgtcattattaatcgatttttctttcttcttccaatataaattattcatacattttttagctctggaagaaacaacgtcgtgatgcagtaaaaaagcagaaagattcatttttcacaaatggaaaacgaaaacggaaagctgatacaggaaacgaagatgctcctgagactgcgtgaaatgttcaagatctaatagttattcaattgttcgttgttgtttttgttgattttcaaatgtttgttatagtaaatacatattttcaaatgttttgatttttttgaaaacttactctggtttaatttccgatgagaataccttttacattttttatgataatttttgttaaactatcataatatttcagaattcttcagcgtaatgtcattttcggatgttttggatgcaaaaccaaaagagccaaagctctatcgattgcttgggtgtgatgaaagctcttcggtaggttaaactttaaaataatattttcataatttaagttatattcagatagatcaaataactgccgaatatcgggcacgggttcgcgactgccatccggataaagtgaaggagcacaacacgaattcgactgaaaaattcatggagctacagggaagtttaataaaataaattaatatcaataaaacgcacaataattgttgttacctcttcagaatgcatattctattttaacgagtgattctcgtcgaaaaacttacgattcgtggcttcattccccgtttccagtttcttttgaagacttcacaaagtctcaggacacttttcagatggtaattttggctccgatatgtgtttttgagtttttttggttcggtccttgtgcttttttgttgaaacttttaattctatttaaaagaaaataaaaaatttcagtcaactcactgggcagttccaaaatctcaaccatctattgcagccaatcaaccagaaaagttgtctcaaaattttaaatctgaaaaatctgaaaaatcacgctggtccgacggtgatcgatatcaatcatcgattgcctcggcttttagaaattataaagtttaaacctgaaattttttgccaaatttaacactccaaaataatatgaatattttcaaaacaatatttgtattttattcatttatatcaattcttaataaatgtttattgaatacttcacctctacgtctttgttcatatttgtaccgtataactcggtttgatattgtttttacgctcataatgagttttcaaatttgaattttagacctttttacttgccagacgattctacaagttttgtatgaacggtgagtttcagatttcgatggctgacacagctcaatacggtataattttcagtctactcgtttgacggtcttgtcgttgctgctctattattcatctgcacctgtgcttatttgaaacgagttccacgtgttagtagctggctgttgtctgagaagaagggattcttcggagttttttataaggtaaagatcagggctaaatccgaaaaaataactttaaaaaaggccttcgaaaaactaaaacttttgcaagttagaaacgttattatttaatattagaataaaagcaaaaatattttaattccgttctagtagagtgtgattttaaaaattgatgattgtttcagttagcgggttgactaacatagctttttttttttacatttttcgtcgttttcgctcactttatttttgaaaatagcaaaaaataacattttcgtgaaaacagtctacttttgactgtttgttttcaaaatgtatgtgtttgtcatatttatatgacgatgaatgagaaaaatgcttattttccctgtaaatatttcacttggaataattagtgtcaagattttatatttgctttcaggctgctgtaattggtgtccgacttcattctctcgttgctctttcgtgtctctctgccgccgtctacgttcttttcgttcgctaaaaacaATGGTTCAAGGAAAGCTCAAGCAAAAGACAGCCTTGCCAAAAGGTGTCAAACAAAAGGTTAAGAAGGCCAAGAATCCAGGACAACCAAAGAAGGGAAAATTCTTGCACATTGCTCCTAAGAAGGCTCATATCATTGAAGCAgtaagtttttcttgtgatcattatcttcattttatttgtcattttcagGAAAAAGTTGCTGCCCATGTCACCAAAGTCATCAACGACAAAAACGAGGAGATGGTGAAAGGCAGAGCTGATACTGCAGTTGGAAAAAATAAGAAGTGAttaagtactctggttatattaatttactgaatttgtctgtcctttgttgaaaataaacttcttttttgtgaactaaattttaattccgcgaatgcataaactacggttctcgttacaattgacacttgaaaagtggattgatctaaataccaagaggatcaatttttcaagaagtgcgcgcgtcccacaaaccaataaaccaatatatactctcgtctacgctgtctttataactttttgttgattttccgccaccgcctacgaaatcacttttttgatgctcttttgttacgaactttatacctgaaaaagatttttatcgcggttgggacgatcgagttcacagtttattttaattttatgtgtatcatcagacgatcgcggatttggtgaccagtaatatattcaaaagtctgttgattttctgaatttttgttaacttcggttttttcaaataacgtcattaaacgatccatgtattttaagctttcattctatgccactttctctttcaacaaattacttgaaatcgaaccattcctgaatcttcatattttacctgcgaataccagctccatgatctaccgccaaaatgctgaccaacaggtatttgtaggcaggcataagatagccacatattaggtaggcataactataggcaagccttcgtacgaacctctccttatcgctcgatccttggagccggtggcaacctcgtttccatcagggtgccgatactttactgcactaaaacataattatgacatcacaatatgaaatactacgcgtatactactgttgtaggaccagaaggacatgtcctgcagcaactcgtgaaaaaactgatcatgttttaaaaaaattctgatttcgaattcatcaccaagcaacgtggttttgttttgatcaaattgaaaaggcacgctagttgttcttctatttttagaaatgaaaactagagtgtaaacgattgatatggtatagatctttgcgatcaaaaatagtttgtagtataggtggtttttatctttgtgctactacaccgaaacgtatgtcgtaattattatattttcgtgaaaaactgaaacttcactttaatgctgttttctgtgtgggtcttgccactatatttgttgtagtgatcgttaaaggtgtgatgtttgacaaaatcggtcattagatgtcgaaattgaagttaacgaaaatggaaagaggtaccagtttgacaattaaatattctgtgcctatgtacgtaccgtatatcctctattagttttgcacccctttgctcaactttggcagctcatatctcagtagtcgtttgttctaaagaaaagttgtcaactgataacatatttgttagatgtttttctatagtttgtagtttgttaattatttatatgtaaaacaaaatgagatatgaactgtcaaagttgagcaaaggggtgcaaaactaatagaggatatacggtatgtagaaattatttttagaaaggattaaaaaattaaaaaatttttttttttaaggattaaaaaatccttttttttttttttttttttttaggattttttaatcctttaaaataaatttggaatatttttgttcacttaatgccatgaccgaaaattctacagtgctgcaaccaagctgccaagaaggctagggaggcgagtggagaggcgccccacgcctcgcctcaatgtcgtgaaagttttaatggcgatgtttcagtaaaataatatagaactataaaagatttttcgtttgtatatttagttccatctttttcaagttttttgttttcaatttttaactattttaatccaatagctatcaagttgtttaggggaaatccctttttcagatcaattcaacagttatcatgtcaacaacatgatgtacgcggtgcaaatcattgttcgctcacattgaaaacacgtttatccataggaggagcgtcactacgaggtcataatctatccctttacttctcgaaatctcattgaaaaactgcacaaagttgaacaccataaacataatcacatgagaagaatcaaaa