# File: K07F5.15_Deletion.ape

## Comments

>K07F5.15.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 5063 bp
>gene: K07F5.15.1; chr: IV; strain: VH7212; allele: hd7202; deletion size: 541 bp; type: Heterozygous; QC: PPHP; outcrossed: 0
>Deletion size = 541 bp
>K07F5.15 sgRNA #1, Chr IV, CAAAAGACAGCCTTGCCAAA; cut=9865831; strand=+1; GC=45; 3'GG=no; folding=-0.5
>K07F5.15 sgRNA #2, Chr IV, AAAAGTGATTTCGTAGGCGG; cut=9866337; strand=-1; GC=45; 3'GG=yes; folding=-0.8

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
K07F5.22_Unspliced_UTR | gene | forward | 2001..2736
K07F5.15-1 SV F | primer_bind | forward | 2098..2119
K07F5.15 Upstream Homology Arm | misc_feature | forward | 2387..2736
Left Deletion Flank | misc_structure | forward | 2707..2736
Selection Cassette | variation | forward | 2737..8153
LoxP | misc_recomb | forward | 2758..2791
Pmyo-2 | regulatory:promoter | forward | 2834..3812
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 3339..3362
GFP(S65C) | CDS | forward | 3840..4010,4062..4215,4267..4430,4482..4709
unc-54 3'UTR | 3'UTR | forward | 4846..5580
Prps-27 | regulatory:promoter | forward | 5779..6572
neoR | CDS | forward | 6573..7367
NeoR SEC Insertion Primer 2 | primer_bind | forward | 6874..6895
unc-54 3'UTR | 3'UTR | forward | 7368..8066
LoxP | misc_recomb | forward | 8097..8130
Right Deletion Flank | misc_structure | forward | 8154..8183
K07F5.15 Downstream Homology Arm | misc_feature | forward | 8154..8603
K07F5.15-1 SV R | primer_bind | reverse | 8913..8935

## Sequence

acctttttacttgccagacgattctacaagttttgtatgaacggtgagtttcagatttcgatggctgacacagctcaatacggtataattttcagtc tactcgtttgacggtcttgtcg ttgctgctctattattcatctgcacctgtgcttatttgaaacgagttccacgtgttagtagctggctgttgtctgagaagaagggattcttcggagttttttataaggtaaagatcagggctaaatccgaaaaaataactttaaaaaaggccttcgaaaaactaaaacttttgcaagttagaaacgttattatttaatattagaataaaagcaaaaatattttaattccgttctagtagagtgtgattttaaaaattgatgattgtt tcagttagcgggttgactaacatagctttttttttttacatttttcgtcgttttcgctcactttatttttgaaaatagcaaaaaataacattttcgtgaaaacagtctacttttgactgtttgttttcaaaatgtatgtgtttgtcatatttatatgacgatgaatgagaaaaatgcttattttccctgtaaatatttcacttggaataattagtgtcaagattttatatttgctttcaggctgctgtaattggtgtccgacttcattctctcgttgctctttcgtgtctctctgccgccgtctacgttcttttcgttcg ctaaaaacaATGGTTCAAGGAAAGCTCAAG ccgcggcgcatcaggcggcct ataacttcgtatagcatacattatacgaagttat tacgtagagctcggtacctcgtacgtaatacgactcactagt acaagtttgtacaaaaaagcaggctta ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGG gtaagtttaaacatatatatactaactaaccctgattatttaaattttcag CCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC gtaagtttaaacagttcggtactaactaaccatacatatttaaattttcag GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTT gtaagtttaaacatgattttactaactaactaatctgatttaaattttcag AACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG cattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcag agatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaac ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG ATCTCCTGTCATCTCACCTTGC TCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA ctcgaggtcgacaggcctgcatgcgtcgac ataacttcgtatagcatacattatacgaagttat agttgcaggaccactgcggccgc ttgatgctcttttgttacgaactttatacc tgaaaaagatttttatcgcggttgggacgatcgagttcacagtttattttaattttatgtgtatcatcagacgatcgcggatttggtgaccagtaatatattcaaaagtctgttgattttctgaatttttgttaacttcggttttttcaaataacgtcattaaacgatccatgtattttaagctttcattctatgccactttctctttcaacaaattacttgaaatcgaaccattcctgaatcttcatattttacctgcgaataccagctccatgatctaccgccaaaatgctgaccaacaggtatttgtaggcaggcataagatagccacatattaggtaggcataactataggcaagccttcgtacgaacctctccttatcgctcgatccttggagccggtggcaacctcgtttccat cagggtgccgatactttactgcactaaaacataattatgacatcacaatatgaaatactacgcgtatactactgttgtaggaccagaaggacatgtcctgcagcaactcgtgaaaaaactgatcatgttttaaaaaaattctgatttcgaattcatcaccaagcaacgtggttttgttttgatcaaattgaaaaggcacgctagttgttcttctatttttagaaatgaaaactagagtgtaaacgattgatatggtatagatctttgcgatcaaaaatagtttgtagtataggtggtttttatctttgt gctactacaccgaaacgtatgtc