# File: K07F5.15_Wildtype.ape

## Comments

>K07F5.15.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 5063 bp
>Deletion size = 541 bp
>K07F5.15 sgRNA #1, Chr IV, CAAAAGACAGCCTTGCCAAA; cut=9865831; strand=+1; GC=45; 3'GG=no; folding=-0.5
>K07F5.15 sgRNA #2, Chr IV, AAAAGTGATTTCGTAGGCGG; cut=9866337; strand=-1; GC=45; 3'GG=yes; folding=-0.8

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
K07F5.22_Unspliced_UTR | gene | forward | 2001..2857
K07F5.15-1 SV F | primer_bind | forward | 2098..2119
K07F5.15 Upstream Homology Arm | misc_feature | forward | 2387..2736
Left Deletion Flank | misc_structure | forward | 2707..2736
K07F5.15 sgRNA #1 | protein_bind | forward | 2737..2756
K07F5.15-1 WT F | primer_bind | forward | 3087..3108
K07F5.15 sgRNA #2 | protein_bind | reverse | 3258..3277
K07F5.15 Downstream Homology Arm | misc_feature | forward | 3278..3727
Right Deletion Flank | misc_structure | forward | 3278..3307
K07F5.15-1 SV R | primer_bind | reverse | 4037..4059

## Sequence

acctttttacttgccagacgattctacaagttttgtatgaacggtgagtttcagatttcgatggctgacacagctcaatacggtataattttcagtc tactcgtttgacggtcttgtcg ttgctgctctattattcatctgcacctgtgcttatttgaaacgagttccacgtgttagtagctggctgttgtctgagaagaagggattcttcggagttttttataaggtaaagatcagggctaaatccgaaaaaataactttaaaaaaggccttcgaaaaactaaaacttttgcaagttagaaacgttattatttaatattagaataaaagcaaaaatattttaattccgttctagtagagtgtgattttaaaaattgatgattgtt tcagttagcgggttgactaacatagctttttttttttacatttttcgtcgttttcgctcactttatttttgaaaatagcaaaaaataacattttcgtgaaaacagtctacttttgactgtttgttttcaaaatgtatgtgtttgtcatatttatatgacgatgaatgagaaaaatgcttattttccctgtaaatatttcacttggaataattagtgtcaagattttatatttgctttcaggctgctgtaattggtgtccgacttcattctctcgttgctctttcgtgtctctctgccgccgtctacgttcttttcgttcg ctaaaaacaATGGTTCAAGGAAAGCTCAAG CAAAAGACAGCCTTGCCAAA AGGTGTCAAACAAAAGGTTAAGAAGGCCAAGAATCCAGGACAACCAAAGAAGGGAAAATTCTTGCACATTGCTCCTAAGAAGGCTCATATCATTGAAGCAg taagtttttcttgtgatcattatcttcattttatttgtcattttcagGAAAAAGTTGCTGCCCATGTCACCAAAGTCATCAACGACAAAAACGAGGAGATGGTGAAAGGCAGAGCTGATACTGCAGTTGGAAAAAATAAGAAGTGAttaagtactctggttatattaatttactgaatttgtctgtcctttgttgaaaataaacttcttttttgtgaactaaattttaa ttccgcgaatgcataaactacg gttctcgttacaattgacacttgaaaagtggattgatctaaataccaagaggatcaatttttcaagaagtgcgcgcgtcccacaaaccaataaaccaatatatactctcgtctacgctgtctttataactttttgttgattttccgcca ccgcctacgaaatcactttt ttgatgctcttttgttacgaactttatacc tgaaaaagatttttatcgcggttgggacgatcgagttcacagtttattttaattttatgtgtatcatcagacgatcgcggatttggtgaccagtaatatattcaaaagtctgttgattttctgaatttttgttaacttcggttttttcaaataacgtcattaaacgatccatgtattttaagctttcattctatgccactttctctttcaacaaattacttgaaatcgaaccattcctgaatcttcatattttacctgcgaataccagctccatgatctaccgccaaaatgctgaccaacaggtatttgtaggcaggcataagatagccacatattaggtaggcataactataggcaagccttcgtacgaacctctccttatcgctcgatccttggagccggtggcaacctcgtttccat cagggtgccgatactttactgcactaaaacataattatgacatcacaatatgaaatactacgcgtatactactgttgtaggaccagaaggacatgtcctgcagcaactcgtgaaaaaactgatcatgttttaaaaaaattctgatttcgaattcatcaccaagcaacgtggttttgttttgatcaaattgaaaaggcacgctagttgttcttctatttttagaaatgaaaactagagtgtaaacgattgatatggtatagatctttgcgatcaaaaatagtttgtagtataggtggtttttatctttgt gctactacaccgaaacgtatgtc