File: K07F5.15_Wildtype.ape

Comments
>K07F5.15.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive
 strand; Sequence: 5063 bp
>Deletion size = 541 bp
>K07F5.15 sgRNA #1, Chr IV, CAAAAGACAGCCTTGCCAAA; cut=9865831;
 strand=+1; GC=45; 3'GG=no; folding=-0.5
>K07F5.15 sgRNA #2, Chr IV, AAAAGTGATTTCGTAGGCGG; cut=9866337;
 strand=-1; GC=45; 3'GG=yes; folding=-0.8


Features
K07F5.22_Unspliced_UTR	gene	forward	2001..2857
 

K07F5.15-1 SV F	primer_bind	forward	2098..2119
TACTCGTTTGACGGTCTTGTCG

K07F5.15 Upstream Homology Arm	misc_feature	forward	2387..2736
TCAGTTAGCGGGTTGACTAACATAGCTTTTTTTTTTTACATTTTTCGTCGTTTTCGCTCACTTTATTTTTGAAAATAGCAAAAAATAACATTTTCGTGAAAACAGTCTACTTTTGACTGTTTGTTTTCAAAATGTATGTGTTTGTCATATTTATATGACGATGAATGAGAAAAATGCTTATTTTCCCTGTAAATATTTCACTTGGAATAATTAGTGTCAAGATTTTATATTTGCTTTCAGGCTGCTGTAATTGGTGTCCGACTTCATTCTCTCGTTGCTCTTTCGTGTCTCTCTGCCGCCGTCTACGTTCTTTTCGTTCGCTAAAAACAATGGTTCAAGGAAAGCTCAAG

Left Deletion Flank	misc_structure	forward	2707..2736
CTAAAAACAATGGTTCAAGGAAAGCTCAAG

K07F5.15 sgRNA #1	protein_bind	forward	2737..2756
CAAAAGACAGCCTTGCCAAA

K07F5.15-1 WT F	primer_bind	forward	3087..3108
TTCCGCGAATGCATAAACTACG

K07F5.15 sgRNA #2	protein_bind	reverse	3258..3277
CCGCCTACGAAATCACTTTT

K07F5.15 Downstream Homology Arm	misc_feature	forward	3278..3727
TTGATGCTCTTTTGTTACGAACTTTATACCTGAAAAAGATTTTTATCGCGGTTGGGACGATCGAGTTCACAGTTTATTTTAATTTTATGTGTATCATCAGACGATCGCGGATTTGGTGACCAGTAATATATTCAAAAGTCTGTTGATTTTCTGAATTTTTGTTAACTTCGGTTTTTTCAAATAACGTCATTAAACGATCCATGTATTTTAAGCTTTCATTCTATGCCACTTTCTCTTTCAACAAATTACTTGAAATCGAACCATTCCTGAATCTTCATATTTTACCTGCGAATACCAGCTCCATGATCTACCGCCAAAATGCTGACCAACAGGTATTTGTAGGCAGGCATAAGATAGCCACATATTAGGTAGGCATAACTATAGGCAAGCCTTCGTACGAACCTCTCCTTATCGCTCGATCCTTGGAGCCGGTGGCAACCTCGTTTCCAT

Right Deletion Flank	misc_structure	forward	3278..3307
TTGATGCTCTTTTGTTACGAACTTTATACC

K07F5.15-1 SV R	primer_bind	reverse	4037..4059
GCTACTACACCGAAACGTATGTC


formatted sequence

        1 ggaatggaag tgacgaagga gacgaagatt caactgaaaa gaaaaaggaa gaagtcgttg
       61 aaaaagacga ggaaagcggt gatgagagct ttgagaaagc tgaaatcttt ttcaaaatgg
      121 atgacgcgtt ttcagctgaa gtgagttttc aacaaaaaaa aacatttttc acacaaaaat
      181 tattttcaga tgaaagaacg aaaagatggt gatgatccgt caactggagg tttctctttt
      241 ttatcaatga ttggaagaaa atatgatgac gaagcagttg aagatgatgg agaaaattta
      301 aagaaaatag aaccaacaca aagtaaagcc gaaccgtctg gaaatgttga caaaatcgaa
      361 aaatctctac tcaaagtgaa agttagcaca aagtttttcg tcgatcctct gtcggatgaa
      421 aaaatcaaaa gtttagcggc taatttcaga agaactcaaa cagtggagaa agttattgat
      481 aaatgggcac ctcatcgaga tgctattttc aaggtgagta gttttttttt tgagcctgac
      541 cgactattga gctctgtttt attatttcaa gcgagtctta atacaaaaaa gatttcaaat
      601 tgttttgatt cagaaagtta taccaatttc cagtatgatc aaataagtcc accatcttaa
      661 taaataacaa aattttccct aactgtctga ttctcgttac gcatacacaa tctcattctc
      721 aatctttttg cggaaaagtg tcattattaa tcgatttttc tttcttcttc caatataaat
      781 tattcataca ttttttagct ctggaagaaa caacgtcgtg atgcagtaaa aaagcagaaa
      841 gattcatttt tcacaaatgg aaaacgaaaa cggaaagctg atacaggaaa cgaagatgct
      901 cctgagactg cgtgaaatgt tcaagatcta atagttattc aattgttcgt tgttgttttt
      961 gttgattttc aaatgtttgt tatagtaaat acatattttc aaatgttttg atttttttga
     1021 aaacttactc tggtttaatt tccgatgaga atacctttta cattttttat gataattttt
     1081 gttaaactat cataatattt cagaattctt cagcgtaatg tcattttcgg atgttttgga
     1141 tgcaaaacca aaagagccaa agctctatcg attgcttggg tgtgatgaaa gctcttcggt
     1201 aggttaaact ttaaaataat attttcataa tttaagttat attcagatag atcaaataac
     1261 tgccgaatat cgggcacggg ttcgcgactg ccatccggat aaagtgaagg agcacaacac
     1321 gaattcgact gaaaaattca tggagctaca gggaagttta ataaaataaa ttaatatcaa
     1381 taaaacgcac aataattgtt gttacctctt cagaatgcat attctatttt aacgagtgat
     1441 tctcgtcgaa aaacttacga ttcgtggctt cattccccgt ttccagtttc ttttgaagac
     1501 ttcacaaagt ctcaggacac ttttcagatg gtaattttgg ctccgatatg tgtttttgag
     1561 tttttttggt tcggtccttg tgcttttttg ttgaaacttt taattctatt taaaagaaaa
     1621 taaaaaattt cagtcaactc actgggcagt tccaaaatct caaccatcta ttgcagccaa
     1681 tcaaccagaa aagttgtctc aaaattttaa atctgaaaaa tctgaaaaat cacgctggtc
     1741 cgacggtgat cgatatcaat catcgattgc ctcggctttt agaaattata aagtttaaac
     1801 ctgaaatttt ttgccaaatt taacactcca aaataatatg aatattttca aaacaatatt
     1861 tgtattttat tcatttatat caattcttaa taaatgttta ttgaatactt cacctctacg
     1921 tctttgttca tatttgtacc gtataactcg gtttgatatt gtttttacgc tcataatgag
     1981 ttttcaaatt tgaattttag acctttttac ttgccagacg attctacaag ttttgtatga
     2041 acggtgagtt tcagatttcg atggctgaca cagctcaata cggtataatt ttcagtctac
     2101 tcgtttgacg gtcttgtcgt tgctgctcta ttattcatct gcacctgtgc ttatttgaaa
     2161 cgagttccac gtgttagtag ctggctgttg tctgagaaga agggattctt cggagttttt
     2221 tataaggtaa agatcagggc taaatccgaa aaaataactt taaaaaaggc cttcgaaaaa
     2281 ctaaaacttt tgcaagttag aaacgttatt atttaatatt agaataaaag caaaaatatt
     2341 ttaattccgt tctagtagag tgtgatttta aaaattgatg attgtttcag ttagcgggtt
     2401 gactaacata gctttttttt tttacatttt tcgtcgtttt cgctcacttt atttttgaaa
     2461 atagcaaaaa ataacatttt cgtgaaaaca gtctactttt gactgtttgt tttcaaaatg
     2521 tatgtgtttg tcatatttat atgacgatga atgagaaaaa tgcttatttt ccctgtaaat
     2581 atttcacttg gaataattag tgtcaagatt ttatatttgc tttcaggctg ctgtaattgg
     2641 tgtccgactt cattctctcg ttgctctttc gtgtctctct gccgccgtct acgttctttt
     2701 cgttcgctaa aaacaATGGT TCAAGGAAAG CTCAAGCAAA AGACAGCCTT GCCAAAAGGT
     2761 GTCAAACAAA AGGTTAAGAA GGCCAAGAAT CCAGGACAAC CAAAGAAGGG AAAATTCTTG
     2821 CACATTGCTC CTAAGAAGGC TCATATCATT GAAGCAgtaa gtttttcttg tgatcattat
     2881 cttcatttta tttgtcattt tcagGAAAAA GTTGCTGCCC ATGTCACCAA AGTCATCAAC
     2941 GACAAAAACG AGGAGATGGT GAAAGGCAGA GCTGATACTG CAGTTGGAAA AAATAAGAAG
     3001 TGAttaagta ctctggttat attaatttac tgaatttgtc tgtcctttgt tgaaaataaa
     3061 cttctttttt gtgaactaaa ttttaattcc gcgaatgcat aaactacggt tctcgttaca
     3121 attgacactt gaaaagtgga ttgatctaaa taccaagagg atcaattttt caagaagtgc
     3181 gcgcgtccca caaaccaata aaccaatata tactctcgtc tacgctgtct ttataacttt
     3241 ttgttgattt tccgccaccg cctacgaaat cacttttttg atgctctttt gttacgaact
     3301 ttatacctga aaaagatttt tatcgcggtt gggacgatcg agttcacagt ttattttaat
     3361 tttatgtgta tcatcagacg atcgcggatt tggtgaccag taatatattc aaaagtctgt
     3421 tgattttctg aatttttgtt aacttcggtt ttttcaaata acgtcattaa acgatccatg
     3481 tattttaagc tttcattcta tgccactttc tctttcaaca aattacttga aatcgaacca
     3541 ttcctgaatc ttcatatttt acctgcgaat accagctcca tgatctaccg ccaaaatgct
     3601 gaccaacagg tatttgtagg caggcataag atagccacat attaggtagg cataactata
     3661 ggcaagcctt cgtacgaacc tctccttatc gctcgatcct tggagccggt ggcaacctcg
     3721 tttccatcag ggtgccgata ctttactgca ctaaaacata attatgacat cacaatatga
     3781 aatactacgc gtatactact gttgtaggac cagaaggaca tgtcctgcag caactcgtga
     3841 aaaaactgat catgttttaa aaaaattctg atttcgaatt catcaccaag caacgtggtt
     3901 ttgttttgat caaattgaaa aggcacgcta gttgttcttc tatttttaga aatgaaaact
     3961 agagtgtaaa cgattgatat ggtatagatc tttgcgatca aaaatagttt gtagtatagg
     4021 tggtttttat ctttgtgcta ctacaccgaa acgtatgtcg taattattat attttcgtga
     4081 aaaactgaaa cttcacttta atgctgtttt ctgtgtgggt cttgccacta tatttgttgt
     4141 agtgatcgtt aaaggtgtga tgtttgacaa aatcggtcat tagatgtcga aattgaagtt
     4201 aacgaaaatg gaaagaggta ccagtttgac aattaaatat tctgtgccta tgtacgtacc
     4261 gtatatcctc tattagtttt gcaccccttt gctcaacttt ggcagctcat atctcagtag
     4321 tcgtttgttc taaagaaaag ttgtcaactg ataacatatt tgttagatgt ttttctatag
     4381 tttgtagttt gttaattatt tatatgtaaa acaaaatgag atatgaactg tcaaagttga
     4441 gcaaaggggt gcaaaactaa tagaggatat acggtatgta gaaattattt ttagaaagga
     4501 ttaaaaaatt aaaaaatttt tttttttaag gattaaaaaa tccttttttt tttttttttt
     4561 tttttaggat tttttaatcc tttaaaataa atttggaata tttttgttca cttaatgcca
     4621 tgaccgaaaa ttctacagtg ctgcaaccaa gctgccaaga aggctaggga ggcgagtgga
     4681 gaggcgcccc acgcctcgcc tcaatgtcgt gaaagtttta atggcgatgt ttcagtaaaa
     4741 taatatagaa ctataaaaga tttttcgttt gtatatttag ttccatcttt ttcaagtttt
     4801 ttgttttcaa tttttaacta ttttaatcca atagctatca agttgtttag gggaaatccc
     4861 tttttcagat caattcaaca gttatcatgt caacaacatg atgtacgcgg tgcaaatcat
     4921 tgttcgctca cattgaaaac acgtttatcc ataggaggag cgtcactacg aggtcataat
     4981 ctatcccttt acttctcgaa atctcattga aaaactgcac aaagttgaac accataaaca
     5041 taatcacatg agaagaatca aaa   
//

plain sequence
 
