File: pigw-1_Wildtype.ape

Comments

>Y110A2AL.12a.1 unspliced + UTR + 2000 upstream + 2000 downstream; -ive strand; Sequence: 11492 bp
>Deletion size = 3569 bp
>pigw-1 sgRNA #1, Chr II, GATTGATAGGCAGACTCCGG; cut=2879222; strand=+1; GC=55; 3'GG=yes; folding=-0.9
>pigw-1 sgRNA #2, Chr II, GATGATGGATGTCGGAGTGG; cut=2882756; strand=-1; GC=55; 3'GG=yes; folding=-2

Features

Name Type Orientation Position
Y110A2AL.1_Unspliced_UTR gene forward 1..1350
pigw-1-1 SV F primer_bind forward 2764..2783
pigw-1 Upstream Homology Arm misc_feature forward 3337..3786
Left Deletion Flank misc_structure forward 3757..3786
pigw-1 sgRNA #1 protein_bind forward 3787..3806
pigw-1-1 WT R primer_bind reverse 5701..5722
pigw-1 sgRNA #2 protein_bind reverse 7336..7355
pigw-1 Downstream Homology Arm misc_feature forward 7356..7705
Right Deletion Flank misc_structure forward 7356..7385
pigw-1-1 SV R primer_bind reverse 9429..9450
Y110A2AL.13.1_Unspliced_UTR gene reverse 10096..11492

Sequence

ttataaatttacaaaaaatattttgatctagtgggaattgaacccatgaccttctgggccttagtcatcgaaaatcccactgcgccatttggactctttttttgaaaattgataaaattcgaaatttgtaacttctgaaatacttccggatgtgtctattaaatgcacctgatagtgagaaaaatatttaaccccgaggggaattgaaatcttggccttctgggtctcaaccattgaaaatcccactgcgccattgggacactttttttgaaaattggtaaaatctgccaaaaaaaattttttccctattttttcgaacgattttaaagttttttcaaagccaaacttcacacattttgtctagtttttttttagaaaatttatccgaattttcagacctttttattttttcaggagtaccgtaggaattgcctgggagagaagcgaacagctgtggaaaatgtcatcaatgcaaaagtggtcgcttggaacctggcagtggtccgtaggcgacactacttcgacctgcacggaatgacgccgcaaggagccgtcgactttgtggctcagatcgttgaagggaggaggccaggctgtatgtttttgagtttacgtgaaagaaaattaaattttaagaatttttgtagttgagaatttttcgatatctcaattttctgccgagttattgaagttttagaaacaactatttgaaaacgaatatatctccccagggtttaaagctatcaaaaaattgttaactagtaaaatatagaaaataaaattctaaacatttaattagttgaaaacttttttctatcttaactctccgccgagatacggcagtttaaagtgagacaaagagagagaggagagacgcagaccgcctcgccgtctgcgtctctcccccctccctcactcttttgcgcactaaacttcaaagccctgtaactttgcaggtatttaagctagaaaaaagtttccaactaacaaaatgtgtgaaattttacgcagagtatgattattttaaaaaaaatcctaaaaattttcagatataaaactcgaaactggacgcggcaaccattccaaggacaacattccggcgattcaaaaccggctgcttcaggattttggcaatctttccggatttcaaatcgccatcgacccgagcaatttaggagttctaattctcagttttcagtgatttgttctgtactattttcgtgtatttcggtgtaatttcatttttgtcatattcttgcagttcatattttgtcattattgtttattgtactattattataatattatttattaatatggtgtacataataaacttgatacaaatttcaaaaacattgagcctccgtatctcaacagtggtttaatttatcaaaaattgccaaaacgcagaatgtagagctaaaaattctgcacatttcaccagttgaaaattttttgatagctctaccgcgagcagagatacagctggattaaggaaacgaggagacgcagacgacgagagaacccacacactctctctctcgcgcgcgcgccaaacttcaaacctctgtaactttgcgagttttacagctagaaaaaaatttccaactatggccatgtgtagaattttaaggcctatctgataatattaatattaaatcaaaactctaacgcggtaaaaactttgaaccttcgtatcttggcaggtattagaattatcggtaaaattacaacagtaaaaatgaggagctaaaaattctgcacatttcatcagttgaaagttttttgatagctcttccctccgcagagatacagctggataaggaaacgactagacgcagacggcgagagagcctgcgtctctcccctcccacactctctctctctctctcgcgcgcgctaaactttgaggctctgtaactttgcgagttttacagctagaaaaaagtttccaactaagaaaatgtgtagaattttaagatctatcagaagaaccattcatctatccaataaaatttattcgaaaaatttcgttgtgagagccgaaaaaacaaataaataagagtttacaagttcgggagatccagaaaaaaaacgatcaaaaaattacagacaatgagtgaaaaaaaaaactagaaatcaggggggggggacaagagacagtgaaagatttggtggccgaggaaatggggaaaactaggccacgtggcagaaaaaatgcaaaaaatacagaaaaagagaggtagaaatctgaatattatgaatgtgaaaaaaaattaaaaattcgagaaaaaaatttaaaatgcattagaaatgtgcgtattttggggggaaataaagaaaggggcggggcgtattaggccacgcccacaaaatggacatcgagctttttgcagaaaaaaagtgagagaaaaaaaaactggaaaactttttttgcgatttttttttgatggccgccaaaaaaaaaactcggccaccaaaacttttttttggtggccgaggtttctctttcacggccatgggaaatttttctttaaaaaatcgcttttttgaaaattttcacaaaaaaaaaaggccgcggccgccaaaaattgtttggtggccgagtttttgtttggcacggccacggaaaaaacaaagattttcccaaaaattcttaaaaataaaaaacatggccgcaaacgaaaaaactcggccaccaaaaatgttttgatggccgatttttctttttggcagccacacgagtcgattttttatcaaaaatttcgatattttcgtgccccccgcgaatgagaaaactcggtcaccaacaaaaattagcttttttttttcggaaaatcgatggcaaaaattgtgaaaattcaatttggaggccgagaaaaaactcggccaccaaaaatttattggtggccgagttttctctttggcggccacgtaaaaaattcgtgaaaatctcgattttcttctaattttttttggcaaaaatttgacttttacatggccaccggtgagaaaactcggccaccaacagaaattagcttttttttgcgaaaaatcgactgcaaaaattgtgaaaattcaatttggaggccgagaaaaaactcggccatcaaaaaatttattggtggccgagttttctctttggcggccacgtaaaaaattcgtgaaaatctcgattttctgctaattttttttggcaaaaacttgacttttacatggccaccgatgagaaaactcggccaccaacagaaattagcttttttttgcggagagaaaaaactcggccaccaaaaatttattggtggccgagttttcatttcggcggccacgtaaaacattttaggcaaaatttaaatttcacatggccgtcaatttgaaaactcggccatcaacaaaatttgcaatatttcaacaaaaaaaatggtacaaattatgcattttccatcattttttcggcgaaaaagcaaaaaaattctctaaaatttcagaaattCTACTCGGCGTGGGGTCTCGTCGCAAATTTAATTTTCCGGTATTCTAAAAAGTGCATGAGACCGGTGCACACAGTGACATATGCCAAGAGAATGGGGAAGGCAATGTGCGGAGGCTGGCGATGAGCATCAACCGAGAGGTTGACAAGGCCTGTGAGCACATTTGAGACGAGGAAAAAGAGCAGTGAGTGGCGATTTATAGAATCAGAAAGACACGGAGACACCACGGAATACGGATCTTCGCCATTTGAGAACTGCGGGAAACTGGAACACCAGGCGACTGTATTGAACATTTGGATTGATAGGCAGACTCCGGTGGCGAAGGTCATGAGGGTTAActgaaagaagaagaaggaattttacatcaaaaagtgctctcacaacgatgctccgttattccgggtgtgtgggcggagcttgttataagcggagtgtcgttgtaaattaattagacattgaacattgctcgatttgcaattttttcgctgagaaaattagaaattcggccaccaaaaaatgtttggcggccgagtttttttggcggccacgctttttcgaattttttttaaatcgttgaaaaagtgattttttaagaaatttatctcgtggccgtgaaactgaaaactcggccaccaaaaaaaaattttggtggccgagtttttttttttttttttcgcgtttttcgtttttatcaatttttgggaaaaaaatcagaaacgtttttgcgtggccgtgcaaagaaaaaaaatctcggccaccgataaaaattttggtggccgacttatttgtaaaaattttcaaaaatattaagcttaaaaaaatcggcaaaaaagttatttttctataaaattttttcgtggccgtgaaagaaaaaactcggccaccaaaaaaaattttacatcaaaaagtgctcacacaacgatgctccgttcttccgtacaaacggagcgtcgttggaaacaaatttgacatcgaaaattgctctcggggggtgatttttggctttttagcccatttttagcccaaaaaatttgaattttggactaaaaattggcctaaaaatcgaattttggacaatttttcgttttttaatttttttttcgtaaaaatcacttgaaaatttcaatttttggcctaaaaatcgactttttctaacttattttagttcagaaagaaatcgacgccgaaaattgcttgctcacaacgatgctccgttcttccgtatcgaaaattggcgattttcaactttttcgcccgaaaaattgatggaaaagtgggaaaattagactttttaagaaatatttttttcaacgcggccgccaaagggaaaactcggccaccattttttttgttgaaaatttgcaaattttgctggtggccgagttttctcattgtcagtgccatgtaaaattctattttttgccgaacatttacgaaatttttttcgtggccgcaaaataactttggtggccgaatttttaggaaaaaatcagaaacttttttcccgtggccgtgcaaaaaaaaaaaaaacttcaccgaaaaaaattttggtggccgaatttttcgtaaaaaaaaaaatatttagcttcataaaatcgttaaaaaatgtgattattctagaaatgtttctttttttctcgtggccgtgaaagagaaaaactcggccaccgaaattttttttttattttttcggatttttcggcaaaaaagtgggaatcaacgccgaaaatcgctcgcaacgacactccgttcttccgcgcgcgcgaggaattaacaaattgatctacCTGTGAAAAGATATACGTGGCATTAACCACACGTCGACACGGTGGGTCAACGCATTTTTCGGCGGCAATTTGAATAAAAAATAGGATCAGCGAGAGTGCGAAGAGCTGGAAGCATCGGCGGATCCACGATTTGATGCGGATGctgaaaaaaaaattttttttttttgtgaaaatttgaattttttcgttttggcgggaattcaaatcacCTAGTCTTCGCCATAAACTCTCCGATGGCCAAACTCGCGTAGAATATCGTCAAATATCCCATGAGTGACGTCAGACCCTCCGCGTTGGCGGTGATCAGATTCTCGCGGCGATTCGCCGACTCGTCAAGGATCCATGCCTGGAGCCCCGTGGATTTGAGCATTGTTTGATGGCAAATTCCAAAGATCACAGAGAGAACGAGAGGGTAGCAGCGTGGCAGAATTGAGGAGGACAGGATctggaagaaaaatttgaaaaaaatctaaaaattccagattgtagtcccaatggatgcagaatctaattctgatcctttctgctttttgaagttttttgatagctctccccattgcagagatacacgcgatcaaagtttcgcggcgagagagtgtatggggaggggagacgcagcaggggtgcgcgccgccgcgtctcccctcctcacacactctctcacctctcagcttcaaatcagtgtatctctgcagcggggagagctatcaaaaatcttccaattgcaaaaatgatcagaattgaattctgcgtcgattggcactaatttccagtggcctagttttccaaaaaaaactcggccatcgtacCCTGATAGCGGCCATCGTGAAGAAAAAGTTCCAATGAACGCCATATTCGGTGACGTGTTGCGGATAGCCAATCACTTCGAGGAGCACAGCACGGCCGGCTCCGAGAGCAAATAGAAGAAATGTGGAGCTGAGGTACCACGGGCGGGGGGAATGGctggaaatttgattttttttttgtgggaaatcgacaacgaaaattgctcacggggtgggtgatttttggcgtttttagcccgtttttaagccaaaaattttgaattttccaactttctcgtcgatttttaggtaaaaatggcctaaaaattggatttttccgctaatttttgagttttttgattagaaatcgacaccaaaaatggctcggcgggtggtttttctgcgtttttagctatttttcggtaaaaaatgctgaaattttgaccttcctcgtcgatttttaggtaaaaatggcctaaaatttgggtttttcgccaattttgcgccaaaattcaattttctacgatttgagcaaaaatctacaacacaaattgctctctgggtggtttttcggcgtttttagccatttttcgagcaaaaattctggatttttggcttttcaaccgctttttgagcatatttggcctaagaaactggattttttcgccaaattttgagcctaaactcaattaaaattttctacgctttgagcaaaaatcgacagcataaattgctcatggggtgattttcgacctttttagaccgttttaagccaataattttgaaattttggcgtttcttatcgatttttaggtaaaaatgggctgaaaactgtatttttaagccattttttgagccaaaactcaattttatacgctttgagcaaaaatcggcaacaaaaattgctcgggggtgattttcgacgttttaggcagtttttcggccaaaaatttcggattttcaggctttcttatcgatttttacataaaaaagccttttaaaaatggatttttaagccaatttttgagccaaaactcaattaacggaaatcgacagcccaaattgttcaattgttcactgggttattttcgtcgctttttagccatttttcgaccaaaaatttcagattttttggcttttcaaccgctttttgagcacatttagccaaaaaaacgtgattttttttcgcggattgctcacTGTGAAAGTTGTCGTCCGGAGCTCGGCGACTTTGCCATTCGGCTGCCTAGCGCCGCCTGAAACAGGAAAGCCGCCACTCCGACATCCATCATCGAATGTCCGAATTTCTCGGTTTTTGCGTATTTTCTCGGAAAAATCGGAAAATCCACAGCCAAAATGGCGAGAGTGACCAAAATCATGGTAAGGGACCGGAAATAGGTGACAAATTGCGTGGGTTTATGCTGTTCTTCGACGAATCGACTCCACGATTGTCGAAGAATCACGGAGAGCTGATGGTGGTTGTGGTGTTGCTCGGCGGCTTCGAAGAGCACAAAAATGAGCAAAACTGCAGAGATGATGATCATTATGGTGGAGAGGAGGATGAGGTGCTCGTTGAGCACTGTCATTGCGAGAAGGGATGGAACGCAGAGGAATATGACGTCGGCAACGAATTTTAGCCAGTAGGTGAATctgcaaaattgggagggaatttgagggaaaaaaagttttttggaattaaaaaaaaaaattttttggactaaaaaaaattgttttcgaaattttttttggaatttttttggaatttaaaactataggttattggctaaaaattcagaaaaatccaaaaaaaaaacattttttttttggaacttttttgaatttttttggaaactgtttggaattttttttcaaggaattttagccagtaggtgaatctgaaaattggggggaatttaagaggggaaaattttttaaacaaattattttttggacttaaaaaaagattttttggaatttttttcaggagtaaaaaatttttttttttgggttctctttaggataaaaattttttttcggaatctctgggtttttttgagcaaattttaggttaaaaagcttaatttttgaaatttttatattgaaatcataataaattggctaaaaaccgaaaaaatccaaaaaaaatcacaaaaaaaaatgtttttgtgattttttttcagattttttttaatttcaaaaaaaaatttcagaactttttttggaatttttttagattattttcagattttttttggaatttaatttgtttttttttttttgaaaattttttggaaatttgggttttttgtgcaaattttaggttaaaaagcttaatttttgaaatttttatattgaaatcataataaattggctaaaaaccgaaaaaatccaaaaaaaatcacaaaaaaaaatgtttttgtgattttttttcagattttttttaatttcaaaaaaaaatttcagaactttttttggaatttttttagattattttcagattttttttggaatttaatttgtatttttttttttgaaaattttttggaaatttgggttttttgtgcaaattttaggttaaaaagcttaatttttgaaatttttatattgaaatcataataaattggctaaaaaaccggaaaaatccaaaaaaaatcacaaaaaaaaatgtttttgtgattttttttcaaaagttttttaatttcaaaaaaaaatttcagaattgtttttggattttttttttggaatttttttggatttttttttggaatttttttggaaaattttttggattttttttggattttttttttttggaatttttttttggcattttttgaaatttcttttttgaaatttctcttttttttggaaaatttcagagtaatttcaaaaaaaatttttccaaattttagtctaaacaaatttaatttcagcatacttaaaactatatgatattagctaaaaatacagaaaaaatccaaaaaaaaaaatccgaaatttgttttttttttttgaattttttttacaacttttttgtttaaaaataaggcgtttttttttcaaattttagtatcaaaaaattgtaagctgtttaattattctttgaaattgggtaaaaaaacagaattttcaaaaataatttttttttggaaaatttggaaaaaatttttttttccaaaaattaaaaaaaaaattgttttcaaaattccaaatttcctcgcggctcaaaattcggccccgcacCTTCCGGTATCATATCGTCGTCCTAAGAAAATCCACGGCAACGTTAGATTCCGTATAAAAAGTCCCAACATTGTGATGAGGAACGTCAAAAAGAGCTCCCGTTGAGCACATCCTGTCAGTGTTCCAACGAATGAACTGTGCTCAGTTGTTGCTCCGACATCGGCTTGGCTCATttaaatgtgtgtctctggaaaaaatgttgaaatttgagaggataaaaatttttttttgaaaaaaaaaattttttttttgagtgttttttaaactgaaattccttaaattttaggcagttttcagcaaattttattcaaaaaataacaaaaatctgttaaatacctttttttttaattaaaaacaattctgaaaaaaacctaattttttcagacattttctgccagttttttctgaaaattcataatttttaaaagtatttttatcgaactttattccaaactgaatgaaaatataatttttaaaattaatttcagacaaaaatttgaaaaaaaataactttcgaaattttttttttcagaaaaaaaattcaaaaaatggttttatttttcaaattttcatttaaaaattctttaaaagattttttcaattttttcagaaacattatttaaaaaaaacgatttttttcaaaattacagagaaaaaatcaaaaatatttttttcaaaaaattttctaaaaatatcttattatctgtgaaaatcactaaaaaatttttttttttaattaaaaaaaaattccattagggctgagacccatatattccacaaaaaaatgaaaaaaaaattttcatcgaaaaaaaaatactatatggaaatttaatcaaaggtacattttgcaaataaaattcaaaaaaaaagtagagagcgaaaaatgggggaaaaactgagatatctggaggaatagcagcggaaaaatcaataaattatcgacttttcaatttaaaaaatcaataaaatatcgattttttagggaaaatggtcaaaaatcgatagattatcgattttttagtgaaaatactgaaaaaatcaataaattatcatttttcgcggagaatttcaaaaaaaaaatcaatttttttagtccaaaaaaaatcattaaaatatcgaatttttagtaaaaattcttttaaaaaacgatttttcaataaaaaatcaataaattatcgatttttcagtgaaaatggtcaaaaatcgattttttagtgtgaaaactctcaataaaatcaataagttatcgatttttcaataaaaaaaatcaataaaatatcgattttttagtgaaaattctataaaaaccgatttttcaattaaaaaaatcaataaattatcgattttttagtgtgaaaactctcgataaaatcaataaaatatcgatttttcagtgaaaataccgaaaaaaaaaatcgatttttcaataaaaaatcaataaaatatcgatttttagggaaaattctttaaaaaaatcgatttttcaataaaaaaatcaataaattatcgatttttcagtgaaaattctattaaaaattgatttttcaataaaaaaatcaataaattatcgatttttccaagaaaatggtcagaaaatcaataaaatatcgatttttagtaaaaatactgaagaaaaaaaacaataaaatatcgatttttccaggcaatttatttgatacgatagatgagatgaacaccggatgaagtatccacaatatcagacatttctccaatttccagtgcaaatgaggcgtcttcgaatggtttctgcatttgacgacgttcgaatggctgaaaattttagagtttttgggttttttggggtgaaaaaatcgatatttttgaagattttttggaggtgaaattagctgaaattgcaggtttttaggcgaaaaattgaccttttttcaagtttttagaggaaaaattggtaaaaatgaattttttcgcagtttttcacgcaaatttatagctaaaatattatattaaaattgattttttttccctgattttcagggtaattttacaggcaatttttcgtgattttgtcataaaattcgaagaaaaattgtatttttcggagcattttaagggtaattttgatatttttaatttttgaacatttttttgtgaatttttgatagcaatttacaaaaaaaacaaagttttccgtgaaaatcgattttt