# File: taap-1_Deletion.ape

## Comments

>M05D6.6.3 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 5164 bp
>gene: M05D6.6.3; chr: II; strain: VH7209; allele: hd7209; deletion size: 1080 bp; type: Homozygous; QC: PPPP; outcrossed: 0
>Deletion size = 1080 bp
>taap-1 sgRNA #1, Chr II, GCTTCATAAATGCATATTCC; cut=8482835; strand=-1; GC=35; 3'GG=no; folding=-1.1
>taap-1 sgRNA #2, Chr II, TTTAATGATACTTTTAGGCG; cut=8483910; strand=+1; GC=30; 3'GG=no; folding=-0.8

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
M05D6.5a.1_Unspliced_UTR | gene | reverse | 112..1828
taap-1-1 SV F | primer_bind | forward | 1495..1516
taap-1 Upstream Homology Arm | misc_feature | forward | 2008..2457
Left Deletion Flank | misc_structure | forward | 2428..2457
Selection Cassette | variation | forward | 2458..7874
LoxP | misc_recomb | forward | 2479..2512
Pmyo-2 | regulatory:promoter | forward | 2555..3533
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 3060..3083
GFP(S65C) | CDS | forward | 3561..3731,3783..3936,3988..4151,4203..4430
unc-54 3'UTR | 3'UTR | forward | 4567..5301
Prps-27 | regulatory:promoter | forward | 5500..6293
neoR | CDS | forward | 6294..7088
NeoR SEC Insertion Primer 2 | primer_bind | forward | 6595..6616
unc-54 3'UTR | 3'UTR | forward | 7089..7787
LoxP | misc_recomb | forward | 7818..7851
Right Deletion Flank | misc_structure | forward | 7875..7904
taap-1 Downstream Homology Arm | misc_feature | forward | 7875..8224
taap-1-1 SV R | primer_bind | reverse | 9362..9385

## Sequence

atttctgtctgtttacttccagaatctgaattccaaatgaccaatgcaaatccgagaaggaaaattagaaaatgtgtaattgtaaacttttcgagcattgcctctaaaaca agaatgactcctggattgctcg cagcatttgagaccactccagtcttctgtgcaccgcctgtgttctttccgctgtggtatccaatgtcgactggaattgcagggatctgaaaaaaattttttttttaatttgaaacaaaatttaactttatattgttttaaataatattttcaaagaactcactccattgtatttctcggcattatccatagttcttgcgagttgttgtggtgtcatatgttcttttttccattcgtaagatcctttcttcggctcctcgtggactccggcgacgttcatgaaagctgtacggaacatgcccattttgtgtga agagagtaattaactataataagaaatagaacttacacaaaaataacaaagaaacgcgtaaatagaaaaattagagcaatataaaatgaattaaaaaaaaaaaaacattgagaagactatatgaatgttcggaccgattgaacggattcatcgaaaactaaaataagattaatcaattt tcaaacatttatgaaacacataatttacaaaaaaagattttagaaaccgtaacaaacgaggagccaaactagtaaaattgaataagaaattgagcgctaaaatttttaatttttgacacgattttaaccgaattatttaagaaaaagcaagatattctactataactgctcatttctaccaatttgtacaatttgattctggtcaaacaaacaaattcgaaatTTACATCTTGGTAACAACTACTCCTGCATCACGATCACGTGCGATTTTCTGAGCCATTGCTCTTTCAGCGATGTAGAATGTTGAAAAGAAGAAAAGTGAAGCAGTGACAATGAAGACTACTCGCATTCTGTCGTGCATTCTGTTCATTGTTCCATGGctgaaattggaaatatttatcttagacaatttatcggctt tttttaatttgaagttttccattaattcca ccgcggcgcatcaggcggcct ataacttcgtatagcatacattatacgaagttat tacgtagagctcggtacctcgtacgtaatacgactcactagt acaagtttgtacaaaaaagcaggctta ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGG gtaagtttaaacatatatatactaactaaccctgattatttaaattttcag CCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC gtaagtttaaacagttcggtactaactaaccatacatatttaaattttcag GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTT gtaagtttaaacatgattttactaactaactaatctgatttaaattttcag AACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG cattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcag agatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaac ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG ATCTCCTGTCATCTCACCTTGC TCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA ctcgaggtcgacaggcctgcatgcgtcgac ataacttcgtatagcatacattatacgaagttat agttgcaggaccactgcggccgc gggtttttcttcgcaagattcacgtttcgg acccgaaaaacgcgcgataagtcacgtaggccgccgtctgtctggtgtctagtgcactaaaatgatgacaaatacgtactctcattctttgaacgttcctcgttgtcaagttcaaatattttctcctaggaatccatatggggattgaggtgcattcaattgttttatgacttacaattagagaaattgaacagtgataggcagtaaagaatgtggatcgattcattgatctagacttattattatgtttagcgaagttgcaacaattttcggatccggctttcaaaacattattgcagcaaataaaaaaataaaatcta cctggaagtccatattaggatggc aggattataagatacagttgcccttcaattaatttaaacatcatttagtcgattattgaaacataaaattcaaaactaataaaatgttttaaatttcttccgctggacataaacct