# File: taap-1_Wildtype.ape

## Comments

>M05D6.6.3 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 5164 bp
>Deletion size = 1080 bp
>taap-1 sgRNA #1, Chr II, GCTTCATAAATGCATATTCC; cut=8482835; strand=-1; GC=35; 3'GG=no; folding=-1.1
>taap-1 sgRNA #2, Chr II, TTTAATGATACTTTTAGGCG; cut=8483910; strand=+1; GC=30; 3'GG=no; folding=-0.8

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
M05D6.5a.1_Unspliced_UTR | gene | reverse | 112..1828
taap-1-1 SV F | primer_bind | forward | 1495..1516
taap-1 Upstream Homology Arm | misc_feature | forward | 2008..2457
Left Deletion Flank | misc_structure | forward | 2428..2457
taap-1 sgRNA #1 | protein_bind | reverse | 2458..2477
taap-1-1 WT R | primer_bind | reverse | 2667..2687
taap-1 sgRNA #2 | protein_bind | forward | 3518..3537
Right Deletion Flank | misc_structure | forward | 3538..3567
taap-1 Downstream Homology Arm | misc_feature | forward | 3538..3887
taap-1-1 SV R | primer_bind | reverse | 5025..5048

## Sequence

atttctgtctgtttacttccagaatctgaattccaaatgaccaatgcaaatccgagaaggaaaattagaaaatgtgtaattgtaaacttttcgagcattgcctctaaaaca agaatgactcctggattgctcg cagcatttgagaccactccagtcttctgtgcaccgcctgtgttctttccgctgtggtatccaatgtcgactggaattgcagggatctgaaaaaaattttttttttaatttgaaacaaaatttaactttatattgttttaaataatattttcaaagaactcactccattgtatttctcggcattatccatagttcttgcgagttgttgtggtgtcatatgttcttttttccattcgtaagatcctttcttcggctcctcgtggactccggcgacgttcatgaaagctgtacggaacatgcccattttgtgtga agagagtaattaactataataagaaatagaacttacacaaaaataacaaagaaacgcgtaaatagaaaaattagagcaatataaaatgaattaaaaaaaaaaaaacattgagaagactatatgaatgttcggaccgattgaacggattcatcgaaaactaaaataagattaatcaattt tcaaacatttatgaaacacataatttacaaaaaaagattttagaaaccgtaacaaacgaggagccaaactagtaaaattgaataagaaattgagcgctaaaatttttaatttttgacacgattttaaccgaattatttaagaaaaagcaagatattctactataactgctcatttctaccaatttgtacaatttgattctggtcaaacaaacaaattcgaaatTTACATCTTGGTAACAACTACTCCTGCATCACGATCACGTGCGATTTTCTGAGCCATTGCTCTTTCAGCGATGTAGAATGTTGAAAAGAAGAAAAGTGAAGCAGTGACAATGAAGACTACTCGCATTCTGTCGTGCATTCTGTTCATTGTTCCATGGctgaaattggaaatatttatcttagacaatttatcggctt tttttaatttgaagttttccattaattcca ggaatatgcatttatgaagc ttttttttttaagattttcaaagaaaattacttacTGAACATATGGTGGAATATCAGCGGCAGACGGGTAAAGTTTGGTAATCACCAAAAATTTTCTCTGCCACTTTGTAGGTACTTGTCCAGTTTCTGAATCGGCTGCTCCAGTAAAACGAGAATCTTGAACAGAATGTTTGAAATCGTCTTTGGTTG CGTAGGTCACACGATGTTGGT tttaatgatacttttaggcg gggtttttcttcgcaagattcacgtttcgg acccgaaaaacgcgcgataagtcacgtaggccgccgtctgtctggtgtctagtgcactaaaatgatgacaaatacgtactctcattctttgaacgttcctcgttgtcaagttcaaatattttctcctaggaatccatatggggattgaggtgcattcaattgttttatgacttacaattagagaaattgaacagtgataggcagtaaagaatgtggatcgattcattgatctagacttattattatgtttagcgaagttgcaacaattttcggatccggctttcaaaacattattgcagcaaataaaaaaataaaatcta cctggaagtccatattaggatggc aggattataagatacagttgcccttcaattaatttaaacatcatttagtcgattattgaaacataaaattcaaaactaataaaatgttttaaatttcttccgctggacataaacct