File: taap-1_Wildtype.ape

Comments
>M05D6.6.3 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 5164 bp
>Deletion size = 1080 bp
>taap-1 sgRNA #1, Chr II, GCTTCATAAATGCATATTCC; cut=8482835; strand=-1; GC=35; 3'GG=no; folding=-1.1
>taap-1 sgRNA #2, Chr II, TTTAATGATACTTTTAGGCG; cut=8483910; strand=+1; GC=30; 3'GG=no; folding=-0.8



Features
M05D6.5a.1_Unspliced_UTR	gene	reverse	112..1828
 

taap-1-1 SV F	primer_bind	forward	1495..1516
AGAATGACTCCTGGATTGCTCG

taap-1 Upstream Homology Arm	misc_feature	forward	2008..2457
TCAAACATTTATGAAACACATAATTTACAAAAAAAGATTTTAGAAACCGTAACAAACGAGGAGCCAAACTAGTAAAATTGAATAAGAAATTGAGCGCTAAAATTTTTAATTTTTGACACGATTTTAACCGAATTATTTAAGAAAAAGCAAGATATTCTACTATAACTGCTCATTTCTACCAATTTGTACAATTTGATTCTGGTCAAACAAACAAATTCGAAATTTACATCTTGGTAACAACTACTCCTGCATCACGATCACGTGCGATTTTCTGAGCCATTGCTCTTTCAGCGATGTAGAATGTTGAAAAGAAGAAAAGTGAAGCAGTGACAATGAAGACTACTCGCATTCTGTCGTGCATTCTGTTCATTGTTCCATGGCTGAAATTGGAAATATTTATCTTAGACAATTTATCGGCTTTTTTTAATTTGAAGTTTTCCATTAATTCCA

Left Deletion Flank	misc_structure	forward	2428..2457
TTTTTAATTTGAAGTTTTCCATTAATTCCA

taap-1 sgRNA #1	protein_bind	reverse	2458..2477
GGAATATGCATTTATGAAGC

taap-1-1 WT R	primer_bind	reverse	2667..2687
CGTAGGTCACACGATGTTGGT

taap-1 sgRNA #2	protein_bind	forward	3518..3537
TTTAATGATACTTTTAGGCG

Right Deletion Flank	misc_structure	forward	3538..3567
GGGTTTTTCTTCGCAAGATTCACGTTTCGG

taap-1 Downstream Homology Arm	misc_feature	forward	3538..3887
GGGTTTTTCTTCGCAAGATTCACGTTTCGGACCCGAAAAACGCGCGATAAGTCACGTAGGCCGCCGTCTGTCTGGTGTCTAGTGCACTAAAATGATGACAAATACGTACTCTCATTCTTTGAACGTTCCTCGTTGTCAAGTTCAAATATTTTCTCCTAGGAATCCATATGGGGATTGAGGTGCATTCAATTGTTTTATGACTTACAATTAGAGAAATTGAACAGTGATAGGCAGTAAAGAATGTGGATCGATTCATTGATCTAGACTTATTATTATGTTTAGCGAAGTTGCAACAATTTTCGGATCCGGCTTTCAAAACATTATTGCAGCAAATAAAAAAATAAAATCTA

taap-1-1 SV R	primer_bind	reverse	5025..5048
CCTGGAAGTCCATATTAGGATGGC


formatted sequence

        1 atttctgtct gtttacttcc agaatctgaa ttccaaatga ccaatgcaaa tccgagaagg
       61 aaaattagaa aatgtgtaat tgtaaacttt tcgagcattg cctctaaaac atgaattttg
      121 ataaatttat tcaaaaaatg agaggtggat gttgagagtg aaaggagcgt acaatataat
      181 tcagcaacag ggaagaattg aaagagatga gacgagggaa aacccgggct tcaaaatatt
      241 tgaaagcaca ttttttggaa aactccaaaa aaaaacctca aatcacggaa ggaaagttct
      301 agtcggtgat gataagagat gaaagcaaat aaaaagagac taatcataag aaaattggaa
      361 aagagagaaa gtatacaatt tattcatttc tggtgctcct catcttcata cgtggcaccg
      421 aaaatggtca ctccggcgac aagagcggtg acagtgaaaa attgggccat aatacggtac
      481 tgcatcattt tttgagctcc gactttgtca ccaagaaatg aggatctata gaaaattttg
      541 aatttttatg tgtttttttt tgataaataa attttagaat ttaagattcc tctaaaattt
      601 cattaaatct taattgccaa aataagttaa cttgatatat tttaaatttt taacaacgat
      661 acactattat taatcacgtg ttcatacatt gtttactaaa gtatagaaca tagacagaga
      721 tcaaaaagtg ttaaaatgag agaaaactgg atttggcaga aaaagtggta gactacactc
      781 tagctaacac tgataaggtc acataaatga aattgattaa tacttgtaaa tactaataaa
      841 aatgtgtata tatatatact aaattagtaa ttgttctcga acgtccgaga ctagtctggt
      901 gtcctctatg ctccgcccaa tttatcggac aggtggtgtc tcatctaatc tgttctctgt
      961 gctcatttgc tcaacacaga agatcaaaag ttcagacaaa ttatgcactc aacgagattc
     1021 acgtgaaaag cacagtaact atatcaaaaa atcacaaaaa tatttgacga cccccggttt
     1081 caacacgaat tcttttaaat acaaacggat gttgttcttt aaagagtagt tttcagtttt
     1141 tgttacagcg gaatttttat cgacttctca tagtttttcg tttcaagtcg tgataaaagc
     1201 catttgaaaa aatcgatgaa aacttggcaa cgacaaaagt ttaaaattgc agtactcttt
     1261 aaaggcgcac acccgtttgt attgaacaaa aaatttcgtg tcgagaccga aaaccgtatt
     1321 tctggggcaa aagtcgcgta acctcgcgtc tgggtaatac aagttttcct aaacatattt
     1381 tcgaaaaggt ttaaattttt agtttttcaa acaacgttta aaactattta tttttaagat
     1441 tttgacactt acttgaacat tcccaaaagg gcggcagttg tcagtcccat tccaagaatg
     1501 actcctggat tgctcgcagc atttgagacc actccagtct tctgtgcacc gcctgtgttc
     1561 tttccgctgt ggtatccaat gtcgactgga attgcaggga tctgaaaaaa attttttttt
     1621 taatttgaaa caaaatttaa ctttatattg ttttaaataa tattttcaaa gaactcactc
     1681 cattgtattt ctcggcatta tccatagttc ttgcgagttg ttgtggtgtc atatgttctt
     1741 ttttccattc gtaagatcct ttcttcggct cctcgtggac tccggcgacg ttcatgaaag
     1801 ctgtacggaa catgcccatt ttgtgtgaag agagtaatta actataataa gaaatagaac
     1861 ttacacaaaa ataacaaaga aacgcgtaaa tagaaaaatt agagcaatat aaaatgaatt
     1921 aaaaaaaaaa aaacattgag aagactatat gaatgttcgg accgattgaa cggattcatc
     1981 gaaaactaaa ataagattaa tcaattttca aacatttatg aaacacataa tttacaaaaa
     2041 aagattttag aaaccgtaac aaacgaggag ccaaactagt aaaattgaat aagaaattga
     2101 gcgctaaaat ttttaatttt tgacacgatt ttaaccgaat tatttaagaa aaagcaagat
     2161 attctactat aactgctcat ttctaccaat ttgtacaatt tgattctggt caaacaaaca
     2221 aattcgaaat TTACATCTTG GTAACAACTA CTCCTGCATC ACGATCACGT GCGATTTTCT
     2281 GAGCCATTGC TCTTTCAGCG ATGTAGAATG TTGAAAAGAA GAAAAGTGAA GCAGTGACAA
     2341 TGAAGACTAC TCGCATTCTG TCGTGCATTC TGTTCATTGT TCCATGGctg aaattggaaa
     2401 tatttatctt agacaattta tcggcttttt ttaatttgaa gttttccatt aattccagga
     2461 atatgcattt atgaagcttt tttttttaag attttcaaag aaaattactt acTGAACATA
     2521 TGGTGGAATA TCAGCGGCAG ACGGGTAAAG TTTGGTAATC ACCAAAAATT TTCTCTGCCA
     2581 CTTTGTAGGT ACTTGTCCAG TTTCTGAATC GGCTGCTCCA GTAAAACGAG AATCTTGAAC
     2641 AGAATGTTTG AAATCGTCTT TGGTTGCGTA GGTCACACGA TGTTGGTCAA CCTCCTGGCC
     2701 TTGTTGTTGT TCGATAATTT GGGCctgaaa atgaataagt atgaaaatag tataatattc
     2761 aaattagctt acCTTTTTTT GAGCCATTTT AGCTTCCAGC GGATCAAATC TTCGAATTGC
     2821 CATACAAACA GCCGGAGTCA TTCGCGATGA GGCTTGCTGT CCTCCGCATC TTATAACCGA
     2881 TGACAGCATt ttgactgaaa attttaattt ttgtcgattt taaatctaaa gtgcaagctg
     2941 accgaagaaa caagaatagt gatgaaaaaa agctgagaaa ccgcccgttt ttgaaacgca
     3001 gcatacgaga gaaatgtagt acacaaactc cgggcgcaac aggaaatagg agagactcag
     3061 tgatgcagag aaatttttta tttcgttgag aagtgcctca gggtaagaaa aaaagtatca
     3121 atatcttctg cattttgaag aaaaataaca gaaaaacacg aataaattgg aattaaatta
     3181 ataataatac agtaccccgc aaagatgaaa ctacgctcta ttgataaact tgaaacgcgg
     3241 tgattcaaat tgaatttttc atcaatagtc tttgaatctg catgccatgt aatttatgta
     3301 ttaattgaaa tgctacaatc tgattaattt tcgaataatt caaacatcaa tttcaaactc
     3361 tctctctttg taaaattatt gaataacgtg gaaataacga gcaacttact tagtactata
     3421 gaatatcaag tagcaagaac ctaaaggaag ataaagataa taaagtaatg agggaccatg
     3481 ttggcaaata ttccttttgt catcattttt cgcatgcttt aatgatactt ttaggcgggg
     3541 tttttcttcg caagattcac gtttcggacc cgaaaaacgc gcgataagtc acgtaggccg
     3601 ccgtctgtct ggtgtctagt gcactaaaat gatgacaaat acgtactctc attctttgaa
     3661 cgttcctcgt tgtcaagttc aaatattttc tcctaggaat ccatatgggg attgaggtgc
     3721 attcaattgt tttatgactt acaattagag aaattgaaca gtgataggca gtaaagaatg
     3781 tggatcgatt cattgatcta gacttattat tatgtttagc gaagttgcaa caattttcgg
     3841 atccggcttt caaaacatta ttgcagcaaa taaaaaaata aaatctaaat ttcacattaa
     3901 attctttttt tttaatttaa aagtttactt actgtgtgtc aaaagacatg tttccaaaat
     3961 tattttcttt atgcgcaaca ttcgtagaaa ctacattgat attaattagg cgttggagaa
     4021 gttctttaaa cattattttt atagtcagtt attcaatagt tcataatcta taatttctaa
     4081 atccgaatgt cttgaattaa attcaaataa attagaaaat gctgtcaatt aaaagcaatt
     4141 tatgcttgtt gactattata aacaatttaa aaagaattac aactagatca gaaaccaagt
     4201 attgattttt aaactgaagg taagaaaaaa aaacgaaata aacacgtatt ctctctcaac
     4261 aacccaacac tctctctttc cactctaaac ctcacacatt ttggatctct ctcccactcc
     4321 acttccccag tctacactat aatcaccact acaatttgtt tacagtcaca cgaactttgc
     4381 acatttgatg caccttttag ttctaaaaaa gtattttcct tgtgagtttc ttatcatctc
     4441 atcaacaaat ttctagagga aaccatctta tcattccctg caagcctaaa tcaaagctga
     4501 tttgttaaaa aggggcgttc ccatttctgg ttatcatttt ttgattattg gcttggaaac
     4561 ataatttcaa caagttgctg ctgaacaagg cggtggattt ctgcacaaaa tttgtgactg
     4621 taccataaag gaagcggaaa aaatcattcc gaggggtttt agatttctca aaatttgtag
     4681 ccatagtttt ggcaaattgc caaattttgg aaaagtagga attcttgaga aatttagaga
     4741 tttgttgaat gtctcaactt ttggttggtt aattattatt gaaaagtgaa tggaatataa
     4801 aaatcgtaga aaaactattt ttggtcgaat taaaaaagta taatatgagt gtcattatga
     4861 tgcgaaggaa attggacacg ggaaaggaaa acccgaactg cccaataaaa ttatcaacac
     4921 atttcaaaaa catttttgaa aagatttagc acaattgaac tgatttcaaa cattttcccc
     4981 cggggtgaga tttcttaaag aattggactg acaactcaga agcacctgga agtccatatt
     5041 aggatggcag gattataaga tacagttgcc cttcaattaa tttaaacatc atttagtcga
     5101 ttattgaaac ataaaattca aaactaataa aatgttttaa atttcttccg ctggacataa
     5161 acct     
//

plain sequence
 
