# File: F43C9.2_Deletion.ape

## Comments

>F43C9.2b.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 10715 bp
>gene: F43C9.2b.1; chr: X; strain: VH7207; allele: hd7207; deletion size: 6980 bp; type: Homozygous; QC: PPPP; outcrossed: 0
>Deletion size = 6980 bp
>F43C9.2 sgRNA #1, Chr X, CACATTCCAATAATACCTCG; cut=4783447; strand=+1; GC=40; 3'GG=no; folding=-0.0
>F43C9.2 sgRNA #2, Chr X, CATCCAGCTGATGCTTTGGG; cut=4790392; strand=-1; GC=55; 3'GG=yes; folding=-2.9

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
F43C9.2-1 SV F | primer_bind | forward | 917..938
F43C9.2 Upstream Homology Arm | misc_feature | forward | 1368..1817
Left Deletion Flank | misc_structure | forward | 1788..1817
Selection Cassette | variation | forward | 1818..7234
LoxP | misc_recomb | forward | 1839..1872
Pmyo-2 | regulatory:promoter | forward | 1915..2893
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 2420..2443
GFP(S65C) | CDS | forward | 2921..3091,3143..3296,3348..3511,3563..3790
unc-54 3'UTR | 3'UTR | forward | 3927..4661
Prps-27 | regulatory:promoter | forward | 4860..5653
neoR | CDS | forward | 5654..6448
NeoR SEC Insertion Primer 2 | primer_bind | forward | 5955..5976
unc-54 3'UTR | 3'UTR | forward | 6449..7147
LoxP | misc_recomb | forward | 7178..7211
Right Deletion Flank | misc_structure | forward | 7235..7264
F43C9.2 Downstream Homology Arm | misc_feature | forward | 7235..7684
F43C9.2-1 SV R | primer_bind | reverse | 8358..8377

## Sequence

tccactggacatttaatcgcgg gcacagggtggcagggcattgatctgtgaaaacctggaattggaaaaattaattgcaaatgaacaaattactttagaaaatataaattgcaacaataagttccgagcaaggctagatggcacttatatccggcaaatgtactttttaggttacaattatcaaaaattactctaattttgttaatgagtatactagcattaatatccaattgactttttcaaaatatcagatattctcttaatcgcgtttttttcaaaattacaataataaatgccacataataaaaaaaaattttctcacgaaaaagttgaatattcaggtatggttttcaatcagtaattttttgtctgtttttttttcttgttgtaaatgttcaatacgcttatatgactatcggcacatattcaaaattctagatgtttgaaaatg cttgaagcgtataggtagccagatggtagtttcattattcattattcaaaaaaattccatattattcctgctcaaactgatatagcaaattgtttgatttattctcaaatttaaatttagttatccgaataagcatggaagtttgactcaacacagccaaaatttctacaattcaaatgaatgtatattactgtacaacaaacaatttcataataccacaacaatttcaactgataccaggtctatccactgaccacaaaacatgttcttctaatgctgggcaaaaacttcttgaaagtttcgaaatgagagctacttacccaaactcaactacatcatcgctcaatcatttcttactctgccgttgtttttgtcttttttagtctctcagccaattcttctgggtattacccgttggat tttgttttgcaacataccgaaactagagta ccgcggcgcatcaggcggcct ataacttcgtatagcatacattatacgaagttat tacgtagagctcggtacctcgtacgtaatacgactcactagt acaagtttgtacaaaaaagcaggctta ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGG gtaagtttaaacatatatatactaactaaccctgattatttaaattttcag CCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC gtaagtttaaacagttcggtactaactaaccatacatatttaaattttcag GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTT gtaagtttaaacatgattttactaactaactaatctgatttaaattttcag AACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG cattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcag agatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaac ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG ATCTCCTGTCATCTCACCTTGC TCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA ctcgaggtcgacaggcctgcatgcgtcgac ataacttcgtatagcatacattatacgaagttat agttgcaggaccactgcggccgc accacgttttcactcaaagcccaccctcct tcactatttttttttccaattttccacatccgtctttgttatatttttctgtcgaaagaagcaaaaaaagtaggatggaaaaaaatagataggaagacggcggcgggaagtttcaaacaccatcgagatattattcatgaagtgaagtgctcttggcacggatacggtggtacaaactttatggaaacttcaaatcccttgagccaaatccatcaaatttgcagaggtgatattcagaaggaaaaaagaaaccaaatccgacatttgggaataaagatacacttcaaggtgtgttgctcgtttgtcatattatatgctcctgcaactgctatcaaagtttggaaaaaaagaaggaaaatgcctttcgggaatacaagcttgtttactgttgatgcagagaaagtacaataaaagtgtagt gctatttcggtcgcgcaatt