File: F43C9.2_Wildtype.ape

Comments
>F43C9.2b.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 10715 bp
>Deletion size = 6980 bp
>F43C9.2 sgRNA #1, Chr X, CACATTCCAATAATACCTCG; cut=4783447; strand=+1; GC=40; 3'GG=no; folding=-0.0
>F43C9.2 sgRNA #2, Chr X, CATCCAGCTGATGCTTTGGG; cut=4790392; strand=-1; GC=55; 3'GG=yes; folding=-2.9


Features
F43C9.2-1 SV F	primer_bind	forward	917..938
TCCACTGGACATTTAATCGCGG

F43C9.2 Upstream Homology Arm	misc_feature	forward	1368..1817
CTTGAAGCGTATAGGTAGCCAGATGGTAGTTTCATTATTCATTATTCAAAAAAATTCCATATTATTCCTGCTCAAACTGATATAGCAAATTGTTTGATTTATTCTCAAATTTAAATTTAGTTATCCGAATAAGCATGGAAGTTTGACTCAACACAGCCAAAATTTCTACAATTCAAATGAATGTATATTACTGTACAACAAACAATTTCATAATACCACAACAATTTCAACTGATACCAGGTCTATCCACTGACCACAAAACATGTTCTTCTAATGCTGGGCAAAAACTTCTTGAAAGTTTCGAAATGAGAGCTACTTACCCAAACTCAACTACATCATCGCTCAATCATTTCTTACTCTGCCGTTGTTTTTGTCTTTTTTAGTCTCTCAGCCAATTCTTCTGGGTATTACCCGTTGGATTTTGTTTTGCAACATACCGAAACTAGAGTA

Left Deletion Flank	misc_structure	forward	1788..1817
TTTGTTTTGCAACATACCGAAACTAGAGTA

F43C9.2 sgRNA #1	protein_bind	forward	1818..1837
CACATTCCAATAATACCTCG

F43C9.2 WT R	primer_bind	reverse	3832..3853
GCCCCAGTTGCCTATTATTTGC

F43C9.2 sgRNA #2	protein_bind	reverse	8778..8797
CCCAAAGCATCAGCTGGATG

Right Deletion Flank	misc_structure	forward	8798..8827
ACCACGTTTTCACTCAAAGCCCACCCTCCT

F43C9.2 Downstream Homology Arm	misc_feature	forward	8798..9247
ACCACGTTTTCACTCAAAGCCCACCCTCCTTCACTATTTTTTTTTCCAATTTTCCACATCCGTCTTTGTTATATTTTTCTGTCGAAAGAAGCAAAAAAAGTAGGATGGAAAAAAATAGATAGGAAGACGGCGGCGGGAAGTTTCAAACACCATCGAGATATTATTCATGAAGTGAAGTGCTCTTGGCACGGATACGGTGGTACAAACTTTATGGAAACTTCAAATCCCTTGAGCCAAATCCATCAAATTTGCAGAGGTGATATTCAGAAGGAAAAAAGAAACCAAATCCGACATTTGGGAATAAAGATACACTTCAAGGTGTGTTGCTCGTTTGTCATATTATATGCTCCTGCAACTGCTATCAAAGTTTGGAAAAAAAGAAGGAAAATGCCTTTCGGGAATACAAGCTTGTTTACTGTTGATGCAGAGAAAGTACAATAAAAGTGTAGT

F43C9.2-1 SV R	primer_bind	reverse	9921..9940
GCTATTTCGGTCGCGCAATT


formatted sequence

        1 agagatacat gtctccggtc tacctgttcg tttaactttt ccaatccttg cattcaactc
       61 tttagtaatt tcatcaactt tcgtgaaagt ttctcgacgt tttagagatg ttatgaatgg
      121 tgctgctgac gacgatggaa gtgccatgga caatctttta ttggagattt cctcaatttc
      181 ttgtgtcaag tgggaggttg acattgaaag tgctttattg aattttgaaa ggttagaatt
      241 acttcgtggt ggaactgaaa ttagtaaatt gttaaataat ttatatttta ctcactgatt
      301 ggttgctcag gatgcggaat cggagtcgct gcacatgaat catgtgagcc ggaagagaat
      361 aacgaggttg aaagtctcga gtcgattgag ttatctctga atttgatacg ttatagtgta
      421 attgaaacgc ataataacct ggataacatc ttcttactgc ctttttcatc aatatcatct
      481 tgttcggcaa gtgcagactc taatctggct ttcaccattt ccaattgttg cgatagctgg
      541 aaacaaattt cttatataca attttctttt aagcaatatt tacaatttca atatctgagc
      601 gtaatctgtt aacatccacc gatttcgcag gatttcttaa catcgtggac gatttgttgt
      661 gcggtggagg tggtggtggg tccattggtc ggggacgggg gagagtggta cttgaccaca
      721 tttttcaatc gaaaattaaa aaaatactaa tgatttattg taaaaattta gtgatagaat
      781 atagtttaaa atgagaatta gcagaaatta ttgttttcaa tcaaaaataa gcaatatcaa
      841 taagcaaaca caaagaaaat caagaccggc gatttgacag cttgtatgac agctctccaa
      901 gcgccgccag gaattgtcca ctggacattt aatcgcgggc acagggtggc agggcattga
      961 tctgtgaaaa cctggaattg gaaaaattaa ttgcaaatga acaaattact ttagaaaata
     1021 taaattgcaa caataagttc cgagcaaggc tagatggcac ttatatccgg caaatgtact
     1081 ttttaggtta caattatcaa aaattactct aattttgtta atgagtatac tagcattaat
     1141 atccaattga ctttttcaaa atatcagata ttctcttaat cgcgtttttt tcaaaattac
     1201 aataataaat gccacataat aaaaaaaaat tttctcacga aaaagttgaa tattcaggta
     1261 tggttttcaa tcagtaattt tttgtctgtt tttttttctt gttgtaaatg ttcaatacgc
     1321 ttatatgact atcggcacat attcaaaatt ctagatgttt gaaaatgctt gaagcgtata
     1381 ggtagccaga tggtagtttc attattcatt attcaaaaaa attccatatt attcctgctc
     1441 aaactgatat agcaaattgt ttgatttatt ctcaaattta aatttagtta tccgaataag
     1501 catggaagtt tgactcaaca cagccaaaat ttctacaatt caaatgaatg tatattactg
     1561 tacaacaaac aatttcataa taccacaaca atttcaactg ataccaggtc tatccactga
     1621 ccacaaaaca tgttcttcta atgctgggca aaaacttctt gaaagtttcg aaatgagagc
     1681 tacttaccca aactcaacta catcatcgct caatcatttc ttactctgcc gttgtttttg
     1741 tcttttttag tctctcagcc aattcttctg ggtattaccc gttggatttt gttttgcaac
     1801 ataccgaaac tagagtacac attccaataa tacctcgtgg tatttcataa aagttttttt
     1861 tacaacacaa cttttcacat gtgttcctta tttagaagtt ctcatgactc aactgtattg
     1921 acactattac aaaatttaac ttgagtcatt gtgtcatttg atcgttttat tcgtttctcc
     1981 tccatttgaa atttttttca ccgcaagatt tgattcatga gagaggttga ggccttcatt
     2041 tttacaccat cgcacctctt ttaaacctgt tttcaattgc cttcttgtat aatcgaatct
     2101 tgagttcctt ctttgaatac taacaaattt cgtcagattc gttttttgac tgatcccgag
     2161 tggccaattg tttgaactgt ttgttacaaA TGACATCTCT CACTCCTGAC Cgtaagttca
     2221 tttttgagtt tcaattataa ataggaaaat atcaatattt taaaggtaga ccagtgccta
     2281 agtaattcaa catattacta cgatgctaaa atagccaact aacataataa acctttaaaa
     2341 aaattagaaa tgttctatag aaaaacaaaa tgtggtgaaa ctgagttagc acaattattt
     2401 tagaatctta aagagctgtc tcattttcaa acccctacaa ttttgaaaaa taaacatttt
     2461 aaacacagaa gaaacataca acaaagtagg atcacatttt ttatccacga cttccaaatt
     2521 caaaatcggt gaaaactgaa atgcacccgc tttatgaaga agattttgaa gattttcaca
     2581 atttttgctt tttgaaaatg attttagccc cataacaagg ttaaaacagt ttacatttga
     2641 ttactacttt tatataacaa agcatgtttc ataggttccc aaataaaact cagttattca
     2701 aaatagaacc tattataaac atcttcgaaa ttatcatgtc tagttttgaa ttctcttgat
     2761 aagtttatga ataatctttt tttttttaac ttttaaattt aaaaaattta aaaaaaaacc
     2821 aaaaaattag tttaaaaata ttttttgctg caactcaatt aaagcttaat ttcgtaaaaa
     2881 aacaatgttt cgatagaagt atgtaattat gatatatgct ttcagttatt ttaactgtaa
     2941 atttcaaaaa gctcaaaaac aaattttatc aatgtttact gagatattcg agcattttag
     3001 cttcagtctc agcagttttc aacaagttcc cctctgacga taatctttta atctataatt
     3061 ttaatttgtt tttgaataga taattgtatg aaaatgttca aaatgctctt catctgattc
     3121 acatttgttg caaaaaaaaa tcaacagaac ttcataatct ttcgttctta gcaaaataag
     3181 ccaactagat acccttgttg gataatagag ttccatattt cattcaaagt acttttttat
     3241 ttgctttttc tttttgtccg ctccttttct acctaccaaa taatctttcg tcagagacta
     3301 tgaacgcaag aatgcactaa ttcatcattt cttgatggaa agttgggaca aaacgtgagg
     3361 acggaaattt ctgtttcaac aacagctgtc gttgtgttgt ctccatccgt cgtcatcttg
     3421 tcaagttttt tctgaatgtc agcaaggcgt tagtattcat gaaacagagc tggagaagaa
     3481 aattttgata atgtcgcaca aagtgcaatt tttgtttgaa aaaagttgca aggaaactat
     3541 gtttatgttg caaaaactat attttggggg ctatagagat attaaaaaca ttcattcaga
     3601 tttgatacgg gcacttcgta aacaaaagtg catacagcgg aagatgaagc acaactttgg
     3661 tcagctagaa tttgccaaaa aagtgttctc tcttgtgtac atttttccag atcttcgaaa
     3721 atgagatgag aactgaaatg ctcagcgtta gcagttacaa aattttggtc gatgcaccat
     3781 gttctttttc acacataacg aaattattgt gatagatcta tgaagtacac agccccagtt
     3841 gcctattatt tgccataaat gtttatttgc ggcaaactgc caaaattgcc aaaaatatta
     3901 gatcaatatt ttttataagc aaaatcactc aaaactatat attttgacac atttgtttgt
     3961 tattatttta ctagttcacg gaatgggatt caattttttt taattaaaaa agttacaagc
     4021 agaaaatttg aaaaacgaaa atatgccatt gtcaaaaaat tcccagtttc ggcaatttcc
     4081 aagtttgccg tttgccgccc accgctgaat ctattttata tttcggaaaa tttagtcgca
     4141 tataaaaaat gtttacaaga aattatagaa catttcagtt gtaatatttt attcaaaatt
     4201 aactttaaat aagctatttt atggtgtgat ttataacatt tcatacaaat tttttatgcc
     4261 tggcaacatc tgacttgcga cctgtggcaa gagcactgac cttttcctgc cctcttgaat
     4321 acatacattc cgccttgaac taatttggcc cgggccacat gcagctcaaa gaatgtgtca
     4381 atgagccaat aaatccatgc atttttgcct ctggccaacg gggcccccaa aacggaggag
     4441 cgcgccttct ccgcctatga tgtctccgcc cattttcaca gctcgctgag caaaacatag
     4501 gcacgacaca tactcaaaag agcatctgcc ctcggtcgcc cctccaaact tctctacttt
     4561 gctaagtttg tttgtgtgtg tgcttcctga agtgattaat tgtattattc aattattgat
     4621 aaataatgac tgctattgct agcccagtaa atcatgatat tatttgccag agatatgtgc
     4681 tagacgattt acgtaagtat acttttctga aatttttttg aatcaaagaa aataataact
     4741 taaaatttga caagcgcaga gaaagcacag aaaaaaatca aacacagttc attgggatta
     4801 ttaaatgaat gtgctcattg tacatcttga attgaaaatg gtacatgtat tatgtatgta
     4861 ttattatgta tgtacaatcc aaacatcacc aaattttgag attgcttttc tagacccaaa
     4921 atagctcaaa acttccactt tttgtttaaa gttttggaaa atatatattt cagctaaaat
     4981 ctcaatatag caaatttaat tgcattacca cactttcata catgttgtgg tcttctaaca
     5041 cgcgtttttt cgttgattga ggtacatttt cagtcgatag gtgcacaaaa ttgtataatt
     5101 tgtgcaacca tcgcctaaaa atgcagctca ataaaattga ataaaatctt aatttagcaa
     5161 gttctgaaaa gctattaaaa tttgagaatt aagatgaaaa taatttgttt ttgtcaaaaa
     5221 ctttgaacgg atcgctacga agtttaggaa gttttagtcc agttagatct aaaaaacttt
     5281 tcgagtgcac tccccatcat cagattacta aaaattgctt tttagacttt tatatacttc
     5341 atatttcttt tatatagtgc tttatagtac ttctagacga acatctaaaa taaacaaatt
     5401 cgcctgatcc ttgcttcttc tcttcccatc gtgctttttc ctgactttcc tgcctctttc
     5461 ctcgaaaatg tggaaatcat aactcagtta tagtgtcaag ttacccaaaa aaaacttttg
     5521 tgtcgtcatc cccaaatgtt tcatctgttt tctttcttgc tctcaacaaa gggcaacaat
     5581 gcgtgaatgt gttgctccaa tcatcagccc tctcgccctg caaaccgaga tctttcctaa
     5641 ttgggaaacg aaactttgcg gagaatgggt tgggagaggg tgcaacacac acaaaattgg
     5701 gtacttgtgt attatttccc ctcatttttt aaaatataat tttaaataaa aacgtgtgga
     5761 gtttttgttt tttaggctta ggaaataacc atttctaagc ctaaagatgg aaaatggaca
     5821 tcacgtttta ttaaaatgga taatattaaa atgaggggaa ataatacaca agtacccaaa
     5881 attgcagtaa ttttgcgagg ttcggtcatt ttttgtgtgg gaggaggaca aactaatgtc
     5941 ataaatcaac atcagaagtg tcatttttag tccaaaaata gaaataaaca ctacttcctg
     6001 gtaaatcaaa ctttgaaaaa aggtgcatgt ataatattgc atatgacacc actatacaac
     6061 acttgtcaga gttaattgaa ttttgaaaaa caccacccca ctccctttat gtcctctcct
     6121 tccattcttt ttcttctcaa taggaatggg tactcgtgta ctttggtcag ttgttttaat
     6181 taagcttaac tgtatcttcc atcgttttct tttagttcca aacaaaactg ccccctcccc
     6241 gtcttagtac agctgcaatt ccacaggggc acattcttca gtgctcctat ctcgagtcat
     6301 gcgcgatagg tgaaacagag agccgaggag gttccagaag agcaaaaggc catccttttg
     6361 ttttatgtga ggagatcttt tgcaatgcag aaatgaaata gaggggtttg agccaaaata
     6421 aataggcggt tttggtaaga ttttgcaagg gaaaaacaat gaaacctttt ctgaataact
     6481 gaaaaaacta gatataggcg gtacctgtta tcgatttttt tttgtgaaca gtttttttta
     6541 gatgtaattg aaaatgcaac acttgcgaat tttcctatta gagttgttgg cacaatgatc
     6601 tactttcacg gcatatatct cggttgtctt tacttttgga aaaaaaagtt ctactactaa
     6661 aatatagaaa aatattttta acgtattttg ttagtaagaa atttttgata caaccactga
     6721 gaaccgagag gggacatgta ataaaaaaaa ctgccaccaa tttcaatcta aatgaaacca
     6781 aatgatagga tctttttttt taatgaccat ttcttctggt atctcagagt gaaaattgat
     6841 tcttaatcgt tgaaactagc ttggctccga ctaacaaaaa aaacgtttac tttaatcgaa
     6901 ttgagtcgcg catgtcccgg aaaggtcttg aatcaacctt cttaatcgaa ttggaatttg
     6961 gtgacttcat gacgcgtttt ctatacaaag aatggctatt ttaaatgtgc gacgccgatt
     7021 ttttgtgatg aattgaaaac agaaagtatc tgagggcaac tgagataaat tttcaatttt
     7081 cattcatgtc cgtaggcgtt ttgtttcggc tcttagagca aagaataggc aaacttgaaa
     7141 agaaaaagaa agagctaaca atgttttgtg aaaaataaaa aattctgtgg tgagagtaaa
     7201 aacaaagtaa tgaattattt tcagAACTCT CAGAGACTTT TGACCTTTTG GATGTTGACA
     7261 AAGATGGGCG GCTTTCGCGG AATGAAATCG CTGCTCTTCT GAGAACTATC AATGTGGAGC
     7321 CAACTCGCGT GGAGTTGGAC TTCATTTTTG GAGAAATGGA TACAGATAgt aagtttaaaa
     7381 ttcttgttta tttgtttcat acaaaacaaa tttgaacaaa aattgacaag tttgtctgtt
     7441 tgtctgtttg tttctctgtg tctccgtgca ggtgtttact gctgaagtgt gtaatccatc
     7501 acagatacca tcggtgtggg cctccttagc tcagttggta gagcgtgaga cttttaatct
     7561 taaggtcagg ggttcgagtc ccctaggtgg cttgattttt tgttggtttt ttgtgcgccc
     7621 tcacatcata ttggtcactg aacttctaca tcttaggtaa tggtccggaa caattgacca
     7681 aacattggtt attttctctg aattttgttg ttctgccagt cacataatag agtagagtga
     7741 agtcgaatgg ttgtcataaa agaaatatta catttctgag caatctttgc acattttaac
     7801 aaaatgtgtg ggcagggaat cactttacgg aagaacatag aactctaaag tatcgctttc
     7861 attcatcatt tgattttttt ccagAAACCG GAAAAATCAG CAAAGAAGAG TTCGTCAACT
     7921 ACATGAAGTC TCCTCCAATT CACAGAACCA CTCTCCGCGA ACTCGAAGTC CAGTTCCGCA
     7981 AGTTTGATTC AGATGGTGAC GGAGCTATCA CTGAAGgttt gttagtttcg tcgcccccaa
     8041 acaattttct gctggaaagt ctccagagag agcgggacac gcaatcattt cttgaaactt
     8101 ttttccatct cctcctttcg caagggaaag tttgtgctct cggtctccca aagttcattt
     8161 tatttcattg tgcagaagga gagccagcga gcgagcaaag caaaaaaaat cgaaaaaaaa
     8221 aagaaaaaga tagggggaaa attggcaaga tagaacgcgc ctattcaatc ttcttgttcc
     8281 acctaaaaac tgttggcagg aattgaaaaa ggaagaagac aaattatacg tttgcatcgc
     8341 ttcctcccgt ttggctcctg cataaaaaat gtgaacactt ttttattttg ctgttttgca
     8401 gACGAGATGG CCGAAATTCT ACGAAGAACC GCTGATCTAG GGGATCGCGC AGCTATCAGC
     8461 GACATGTTCA AGGCGACTGA TTTGAATGGG GATGGTAAAA TTACCTTCTT CGAGTTTGTG
     8521 AAAATGATGC AAGAATAGat aaatttacct gagaatacac ctaaattaat atgaatcgca
     8581 tttacacccc cacttctatt tcatctgtcc tgtcgtgttt aagctcacca ccccgtgttg
     8641 ttcattcctt tccagaatct tctttttttt aatgtaccac ttttaccact gaataaacta
     8701 gtcgcacttt caacactata caaagtttca catgcttcat ccaccctcgc ccaacaacaa
     8761 caacaacaaa atgcccaccc aaagcatcag ctggatgacc acgttttcac tcaaagccca
     8821 ccctccttca ctattttttt ttccaatttt ccacatccgt ctttgttata tttttctgtc
     8881 gaaagaagca aaaaaagtag gatggaaaaa aatagatagg aagacggcgg cgggaagttt
     8941 caaacaccat cgagatatta ttcatgaagt gaagtgctct tggcacggat acggtggtac
     9001 aaactttatg gaaacttcaa atcccttgag ccaaatccat caaatttgca gaggtgatat
     9061 tcagaaggaa aaaagaaacc aaatccgaca tttgggaata aagatacact tcaaggtgtg
     9121 ttgctcgttt gtcatattat atgctcctgc aactgctatc aaagtttgga aaaaaagaag
     9181 gaaaatgcct ttcgggaata caagcttgtt tactgttgat gcagagaaag tacaataaaa
     9241 gtgtagtcta caaaaacata aaatcagaaa acaggaaaaa atgcgctaaa aaaactatta
     9301 ctagcgtttt caagaaacct ttgaagcttt aacagtttca aataaaaatg caacatggtg
     9361 atcccacaaa aaatatgtac gatattttat tttatctaaa ttgttaagct tgtacgaagg
     9421 gaaatgaaat ttttatgtca agtcaacttg gttgaacatt tgaaaaaacc gatatccggg
     9481 tccgttttct aacggattcc gtgccacact acatgtattt cgtgtattaa aaaaagaacc
     9541 cactaaagaa taacaattca ggatagttca tcatgggtta gattaggtac tgaactaatt
     9601 cttatattct caaaacaatt aatgtttttt ttttattata caagtgatgc aacataaaaa
     9661 atgggggttt tttacaaacc gaaatgacaa actggtgcac tgaacattta ttccaaacaa
     9721 tttttgaaag ctagcatttt tctaatcccg aaaattattg agatctataa tatgtcgatt
     9781 tcgaataaca tgaccacata tttccaaacg ggattttttt gacaggatgg attcggacgg
     9841 atggacaatc tggatgggtt tttttttcgg tattaactac atactcaaca aatttacata
     9901 gaactgaaaa agggaatttc gctatttcgg tcgcgcaatt ggcggtgcaa attttctgta
     9961 tcaggggtgt gcggataaca gccgaattag ccgaattagc cgatcggcta attcggctag
    10021 ctcggctaat attagccgaa ttatccgtta gccgaccttt ttaccgatta gccgaattag
    10081 ccgttagccg actttttggc agttcggcta atacgtagtt atccgttagc cgattttggc
    10141 cgatgttagc cgaattcgcc gatcggctat ttcggctaat tcaactaaca tatttttttg
    10201 gaaaacaaaa tgtggtgaat ttgtatgatt ttttcctatt tgaacagtcg ttcatccctg
    10261 aatttgctca ataacaatga aaaaaataac ccaaaacctg aattaacaga atggacaaaa
    10321 aaagcttaac caaatgaata ttttccgaat tagccgaatt agccgaaaat tagccgattc
    10381 gaccgataaa ttttagccga gttatccgtt agccgaaaat tttgaaatta gccgttagcc
    10441 gaattatccg ttagtcgatt gttaaaaaat cggcgttagc cgatttatcc gttagccgaa
    10501 aaaatcggtt atccgcacac ccctgttctg tatcttgaag ggttcgcttg gaaatctagt
    10561 ttccgccgca ctatccacaa gggaacttgg aatttgaaaa ataaaaatag ttgtccaaat
    10621 ttaaataaag tataagattg tgtatatatt gaacagcgga aaacactttt taatcggcca
    10681 ataaaacaaa tctgggatat gttctttttg taatt  
//

plain sequence
 
