# File: cyp-13A5_Wildtype.ape

## Comments

>T10B9.2 unspliced + UTR + 2000 upstream + 2000 downstream; -ive strand; Sequence: 6086 bp
>Deletion size = 1318 bp
>cyp-13A5 sgRNA #1, Chr II, TTCCAAAGCGAAGATCAAAT; cut=9798882; strand=+1; GC=35; 3'GG=no; folding=-0.4
>cyp-13A5 sgRNA #2, Chr II, TCTCCCAATTTCAACAGTCC; cut=9800180; strand=+1; GC=45; 3'GG=no; folding=0.0

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
cyp-13A4_Unpliced_UTR | gene | forward | 1..1464
cyp-13A5 SV F | primer_bind | forward | 1698..1719
cyp-13A5 Upstream Homology Arm | misc_feature | forward | 2139..2588
Left Deletion Flank | misc_structure | forward | 2559..2588
cyp-13A5 sgRNA #1 | protein_bind | forward | 2589..2608
cyp-13A5 WT F | primer_bind | forward | 3006..3027
cya-13A5 sgRNA #2 | protein_bind | forward | 3887..3906
Right Deletion Flank | misc_structure | forward | 3907..3936
cyp-13A5 Downstream Homology Arm | misc_feature | forward | 3907..4356
cyp-13A5 SV R | primer_bind | reverse | 4642..4663

## Sequence

t agttggaaaaaatgcttgattttgctatttagagtatggtgtttatatacagatcagtttgtgtactgtggttcagtattgtctgctttcacacatttgtgtttcgcgtcgcaacaattatggtttgtgttttagaaggaaacatggatgatagcagagagcacgacaatttctgaacgtaggttgataaattggaaaaattatcaactcctatgcattgttggaaactgtta tgttgtgtcgacatcatacggg caggattccgtacttgaagttagttttgaccatacaaacgaactcttatttccacttatctctctaatatgcattctattttctgttagttatttttttgaaatgatgttttaaaatatgttttctaatttaaatgatttcctaacgtgtagatacattaggacgagtaaagtaattttttattagaaaagtgatttaaaattagaagcaagcacatgagctggttttcaatgttaataaattgtctttcttgaaggattctgcaattttttaaattagctatcgatttgaacaatttattaatcaaaaaaccaataggtacacatatgcataatttattatacaactaaaattaggaaTTAATTGGAATGTCTTGGTGTCAACTTGAGCATAACACTTCTTGGTGCAGTTGTGGCGGA TCCGACCAATTTTAATGGAATTTCTGTTTGCGTAGAAGTTTCGAATGTGTACTTTTTCAGGATGTGGGTCAAAAGCATTTTTTTTTCCATCATTGCCAATCGCATTCCAATACAAATTCTTGGTCCCATACCGAATGGAAGATATCCTCCTTTTGCATAAAATAGTTCGTCTCCAGATTCCCATctgcaaaaagtgatgttctttgggaagaaaaaattaaacttgaagctcatagctcatcttattaagaacatagaacatacCTCTCTGGCCTAAACTCATTTGCATCCTCTCCCCAAACTTTTGGATCATAATGAAGTGTCCATGTATCCACTTGAACATTTGTTCCTTTCTCTATTTGAACTCCAAGTACATTCGTTTCCTTCATGCACTTTCGGTTGTGAACGctgaaaatcgaa ttgacctgcttagattattaatagaatact ttccaaagcgaagatcaaat agggaattgcattaaacctaatgaattttaaatcttcttgaattaaaaattcaaattataaatagagtcaacggataacttgaaacatctaaaagcccgctaataaaatacttacATGGATGCTAATGGATACATTCGTAGAGCTTCCTTTACCACACATTCCATGTACTTCAATTTTGATATTTGATCAAAAGTGACTTCAGGATTTGGGCATTCTCTATCCACTTCTTCTTGTAATTTCTTCTGAATCTCTGGATGTCTTGCCAGGAGATATGAAGAATATGAGAGGGAAAGGGCAGTTGTATCATATCCAGCTAACAGAAAAACGAACAATTGGCCAATAATTTCATCAAATGTCAGTTGTTTATCAACTTTCGCAATTTCTGTTTTAGCAAAT CCTAGTGCGGATTCCTCTTCAA TCTCCCAATTTCAACAGTCC TGGATTTTCATAGTCTTGGAACTCGTGAAT CACTCCAAAAAATGGAAATCCACGGGGTCCTTTGACTCCTTTCCTGATCCAAAATGACCAAATCCAGATATAATATGTCAAAAGTCCAATAAACGAAGCACCAGCAATAAGTATACTTAAACTCATattttggaacaaaatatagtgtttcttctatctagtccagagagtatttatatactaaccgacatatttaccaaggccgtggtcacaaaaatcatttgacttgcttactcactcgggtgtttctcggcgcaacaagtcactatgatttgtgtttgaaaaatgggaaatgaatgacggaaaatttgtgacttggaattggacagaagtgaaacaagatagtgtttagatgatgattgattagctaggtaattcatttatgatatttattgttagagttttttgaagttgttcaag tagaataatgttctatactccaaagacaacaacattttttttttcatttttttgtaaaccatttttttgtaaactatcagtttgtttaactgttttcaatattaaatgttcaatataaaattatgtttgattttattttgatttatttatggctatttttcaattaaaacatatcagtaaaataaatattaattagacaatttttatacatgtaaaattacaaaaaattatgaatctctcggctccaatttcacaattacactttccggcgaaagtgtcaacttt cctaccaacttcaccggaatct