File: cyp-13A5_Wildtype.ape

Comments
>T10B9.2 unspliced + UTR + 2000 upstream + 2000 downstream; -ive strand; Sequence: 6086 bp
>Deletion size = 1318 bp
>cyp-13A5 sgRNA #1, Chr II, TTCCAAAGCGAAGATCAAAT; cut=9798882; strand=+1; GC=35; 3'GG=no; folding=-0.4
>cyp-13A5 sgRNA #2, Chr II, TCTCCCAATTTCAACAGTCC; cut=9800180; strand=+1; GC=45; 3'GG=no; folding=0.0


Features
cyp-13A4_Unpliced_UTR	gene	forward	1..1464
 

cyp-13A5 SV F	primer_bind	forward	1698..1719
TGTTGTGTCGACATCATACGGG

cyp-13A5 Upstream Homology Arm	misc_feature	forward	2139..2588
TCCGACCAATTTTAATGGAATTTCTGTTTGCGTAGAAGTTTCGAATGTGTACTTTTTCAGGATGTGGGTCAAAAGCATTTTTTTTTCCATCATTGCCAATCGCATTCCAATACAAATTCTTGGTCCCATACCGAATGGAAGATATCCTCCTTTTGCATAAAATAGTTCGTCTCCAGATTCCCATCTGCAAAAAGTGATGTTCTTTGGGAAGAAAAAATTAAACTTGAAGCTCATAGCTCATCTTATTAAGAACATAGAACATACCTCTCTGGCCTAAACTCATTTGCATCCTCTCCCCAAACTTTTGGATCATAATGAAGTGTCCATGTATCCACTTGAACATTTGTTCCTTTCTCTATTTGAACTCCAAGTACATTCGTTTCCTTCATGCACTTTCGGTTGTGAACGCTGAAAATCGAATTGACCTGCTTAGATTATTAATAGAATACT

Left Deletion Flank	misc_structure	forward	2559..2588
TTGACCTGCTTAGATTATTAATAGAATACT

cyp-13A5 sgRNA #1	protein_bind	forward	2589..2608
TTCCAAAGCGAAGATCAAAT

cyp-13A5 WT F	primer_bind	forward	3006..3027
CCTAGTGCGGATTCCTCTTCAA

cya-13A5 sgRNA #2	protein_bind	forward	3887..3906
TCTCCCAATTTCAACAGTCC

Right Deletion Flank	misc_structure	forward	3907..3936
TGGATTTTCATAGTCTTGGAACTCGTGAAT

cyp-13A5 Downstream Homology Arm	misc_feature	forward	3907..4356
TGGATTTTCATAGTCTTGGAACTCGTGAATCACTCCAAAAAATGGAAATCCACGGGGTCCTTTGACTCCTTTCCTGATCCAAAATGACCAAATCCAGATATAATATGTCAAAAGTCCAATAAACGAAGCACCAGCAATAAGTATACTTAAACTCATATTTTGGAACAAAATATAGTGTTTCTTCTATCTAGTCCAGAGAGTATTTATATACTAACCGACATATTTACCAAGGCCGTGGTCACAAAAATCATTTGACTTGCTTACTCACTCGGGTGTTTCTCGGCGCAACAAGTCACTATGATTTGTGTTTGAAAAATGGGAAATGAATGACGGAAAATTTGTGACTTGGAATTGGACAGAAGTGAAACAAGATAGTGTTTAGATGATGATTGATTAGCTAGGTAATTCATTTATGATATTTATTGTTAGAGTTTTTTGAAGTTGTTCAAG

cyp-13A5 SV R	primer_bind	reverse	4642..4663
CCTACCAACTTCACCGGAATCT


formatted sequence

        1 ttggttttct tcatgcactt tcggttgtga actctgaaaa tatacttttt gaaaaaaagc
       61 aaacaaattt gtaatagcgg gtaaattgga atatctagaa tttaataaac gataataaaa
      121 aacttacaag gatgctaaag ggtacattcg tagagcttct tttaccacac attccatgta
      181 cttcagtttt gatatctgat caaaagtgac ttcagggtct gggcattctc tatccacttc
      241 ttcttgtaat ttcttctgaa tctctggatg tgttgccagg agatatgaag aatatgacag
      301 agaaagggca gttgtatcat atccagctaa caagaaaaca aacaattgac caatgatctc
      361 atcaaatgtc agatgcttat ccactttcaa gacttcactt tttgcaaatc ctaatgtcga
      421 ttcctcttca ataaaatcta cgtttgccct tgcgtctagg aacaagtcga taaaatcttg
      481 tggttcacca gatggctcaa ttccagcttt tttgtctgct gctctttgtt ctagtcgttt
      541 gttgagagct ttttccatgg tattcattat accgaaagcc ggactaaatt ttgggaattt
      601 catgaaaagt tggcgcatca aagttcctgc aattgggaaa acaccagcaa ttaggaaagg
      661 cattttacca ccctttttga aaatctggaa gttttttatt tgcaaattgg taaaatttct
      721 tacaaagtac aacttacttc ttttacctta ggaagcattg gatttctaaa catcagcgat
      781 tcggtttgac ccattgcaat tcttccaatg atgtcaagag tgaattcttg atagtagctg
      841 aaaaaggttt ctgcatcgtc tcgaaaattt aaacgaaaaa ctcactctaa taaatctaca
      901 gcttttcctt ttgcacttgc ctcatccaaa tgtctcatca actccactac agtttcctct
      961 acagtactca taatttttct gagactctta ttcgaaaacg taggcgaagc caatgttctc
     1021 aaccgcttcc atcggtgccc ttgagccaag acaaggtgtg ctcttttgtt tttatttggg
     1081 tctccttgaa tgggatttgt ctttcttcca tagaaattat caaattgttt cacaaaaact
     1141 tcgtggacaa attctggatt agacacaacc aaagtttttt cgacaccttc agtgatacca
     1201 tagattgatc catatttctg gaagattgat gatagtttat atataactct gtatcaataa
     1261 aaagaagcaa acactcactt ttgtccattc tcccaacttc aaaagtcctg gattttcata
     1321 gtcatgaaac ttgagaatca ctccgaaaaa tggaaatcca cggggtcctt tgactccttt
     1381 cctgatccaa aatgaccaaa tccagatata atatgtcaaa aaaccaataa acagagcacc
     1441 ggcaataagt aaacttaaac tcatagttgg aaaaaatgct tgattttgct atttagagta
     1501 tggtgtttat atacagatca gtttgtgtac tgtggttcag tattgtctgc tttcacacat
     1561 ttgtgtttcg cgtcgcaaca attatggttt gtgttttaga aggaaacatg gatgatagca
     1621 gagagcacga caatttctga acgtaggttg ataaattgga aaaattatca actcctatgc
     1681 attgttggaa actgttatgt tgtgtcgaca tcatacgggc aggattccgt acttgaagtt
     1741 agttttgacc atacaaacga actcttattt ccacttatct ctctaatatg cattctattt
     1801 tctgttagtt atttttttga aatgatgttt taaaatatgt tttctaattt aaatgatttc
     1861 ctaacgtgta gatacattag gacgagtaaa gtaatttttt attagaaaag tgatttaaaa
     1921 ttagaagcaa gcacatgagc tggttttcaa tgttaataaa ttgtctttct tgaaggattc
     1981 tgcaattttt taaattagct atcgatttga acaatttatt aatcaaaaaa ccaataggta
     2041 cacatatgca taatttatta tacaactaaa attaggaaTT AATTGGAATG TCTTGGTGTC
     2101 AACTTGAGCA TAACACTTCT TGGTGCAGTT GTGGCGGATC CGACCAATTT TAATGGAATT
     2161 TCTGTTTGCG TAGAAGTTTC GAATGTGTAC TTTTTCAGGA TGTGGGTCAA AAGCATTTTT
     2221 TTTTCCATCA TTGCCAATCG CATTCCAATA CAAATTCTTG GTCCCATACC GAATGGAAGA
     2281 TATCCTCCTT TTGCATAAAA TAGTTCGTCT CCAGATTCCC ATctgcaaaa agtgatgttc
     2341 tttgggaaga aaaaattaaa cttgaagctc atagctcatc ttattaagaa catagaacat
     2401 acCTCTCTGG CCTAAACTCA TTTGCATCCT CTCCCCAAAC TTTTGGATCA TAATGAAGTG
     2461 TCCATGTATC CACTTGAACA TTTGTTCCTT TCTCTATTTG AACTCCAAGT ACATTCGTTT
     2521 CCTTCATGCA CTTTCGGTTG TGAACGctga aaatcgaatt gacctgctta gattattaat
     2581 agaatacttt ccaaagcgaa gatcaaatag ggaattgcat taaacctaat gaattttaaa
     2641 tcttcttgaa ttaaaaattc aaattataaa tagagtcaac ggataacttg aaacatctaa
     2701 aagcccgcta ataaaatact tacATGGATG CTAATGGATA CATTCGTAGA GCTTCCTTTA
     2761 CCACACATTC CATGTACTTC AATTTTGATA TTTGATCAAA AGTGACTTCA GGATTTGGGC
     2821 ATTCTCTATC CACTTCTTCT TGTAATTTCT TCTGAATCTC TGGATGTCTT GCCAGGAGAT
     2881 ATGAAGAATA TGAGAGGGAA AGGGCAGTTG TATCATATCC AGCTAACAGA AAAACGAACA
     2941 ATTGGCCAAT AATTTCATCA AATGTCAGTT GTTTATCAAC TTTCGCAATT TCTGTTTTAG
     3001 CAAATCCTAG TGCGGATTCC TCTTCAAAGA AATCTACATT TGCTCTTGCG TCTAGGAACA
     3061 AATCAATGAA ATCTTGTGGT TCACCAGATG GCTCAATTCC AGCTTTTTCG TCTGCGGCTC
     3121 TTTGTTCCAG TCGTTTATTC AAAGCTTTTT CCACAGTACT CATAATATCG AAGGCTGGTT
     3181 GTATAGATGG GAATCTCATG AAGAATTCGC GGAACATAGT TCCCGCTATC GGGAAAATAC
     3241 CAGAAACTAG GAAAGGTAGT TTACGACCAT CTTTGAAAAT ctaaaattat aatttttgtt
     3301 atgcttttga tggaattcag tacaaaatgc aacttacTCC TTTCACCTTA GGAAGCATTG
     3361 GATTTCTAAA CATCAGCGAT TCTGTTTGAC CCATTGCAAT TCTTCCAATG ATGTCAAGAG
     3421 TGAATTCTTG GTAGTAGctg caaattgttt ttttgtaaat gttttgcatc ttctcaatat
     3481 ttttaattaa agacttacTC TAATAAATCT ACAGCTTTTC CTTTTGCACT TGCATCGTCC
     3541 AAATGTCTCA TCAACTCTAC TACAGTTTCT TCTACAGTAC TCATAATTTT TCTGAGGTTT
     3601 TTATTTGAAA ACGTAGGTGA TGACAGAGTT CTCAACCGCT TCCAACGGTG CCCTTGAGCC
     3661 GAGACAAGAT GTGCTCTTTT GTTTTTATTT GGGTCTCCTT GAATGGGATT TGTCTTTCTT
     3721 CCATAGAAAT TATCAAACTG TTTCACAAAT ACTTCATGAA CAAATTCTGG ATTAGACACA
     3781 ATCAAAGTTT TTTCGACTCC TTCAGTAATA CCATATATTG GTCCGTATTC ctgaatatgt
     3841 gactcgtttt tggtaaacac ataattttaa actcacTTTT GTCCATTCTC CCAATTTCAA
     3901 CAGTCCTGGA TTTTCATAGT CTTGGAACTC GTGAATCACT CCAAAAAATG GAAATCCACG
     3961 GGGTCCTTTG ACTCCTTTCC TGATCCAAAA TGACCAAATC CAGATATAAT ATGTCAAAAG
     4021 TCCAATAAAC GAAGCACCAG CAATAAGTAT ACTTAAACTC ATattttgga acaaaatata
     4081 gtgtttcttc tatctagtcc agagagtatt tatatactaa ccgacatatt taccaaggcc
     4141 gtggtcacaa aaatcatttg acttgcttac tcactcgggt gtttctcggc gcaacaagtc
     4201 actatgattt gtgtttgaaa aatgggaaat gaatgacgga aaatttgtga cttggaattg
     4261 gacagaagtg aaacaagata gtgtttagat gatgattgat tagctaggta attcatttat
     4321 gatatttatt gttagagttt tttgaagttg ttcaagtaga ataatgttct atactccaaa
     4381 gacaacaaca tttttttttt catttttttg taaaccattt ttttgtaaac tatcagtttg
     4441 tttaactgtt ttcaatatta aatgttcaat ataaaattat gtttgatttt attttgattt
     4501 atttatggct atttttcaat taaaacatat cagtaaaata aatattaatt agacaatttt
     4561 tatacatgta aaattacaaa aaattatgaa tctctcggct ccaatttcac aattacactt
     4621 tccggcgaaa gtgtcaactt tcctaccaac ttcaccggaa tctgtgtatt ttttgttgtt
     4681 ctgaatgagt attctttgag aatctgagcc aataaaatct tctgctccat ttgagctagt
     4741 ctcattccaa tgcactgacg tggtcccgag ccaaatggaa tgtacgatcc attgtgctcc
     4801 aaatgctcac aagctccgtt ttcccacctg taatatagaa gattgtgatg aaaacaagat
     4861 cactaagatt acctttcggg tttgaagtct tctacgtctt ctccccaaat tcttggattg
     4921 tgatgtagtg tccatgtgtc aacttgaatg ttcattccct catcaaaatt caccccgttg
     4981 attgttgtct ctcgcataca ttttctcgtg tttgctctgt aagattatat tgttctcact
     5041 tttttttaat tttaagccaa aaatagtctt gtcaacttac gtcgttccta atggatataa
     5101 tctcaacgtt tctttcatta cacactccaa atatttcagt ttcgataaat gatcaaagaa
     5161 gatttcggga tctggacact ctctgtccac ttcttcttgt aattttgttt ggatttctgg
     5221 atgtgttgca agaagatatg tagcatatga gagagataat gcagttgtgt caaagccagc
     5281 agctaaaaat acgaaacact gtgcaataat ctcatcgaaa gtcaactcct tgttgatttt
     5341 tacaacgctc gattttgcaa aatcttcatt cgcctctccg ctcaatattt tcacgtctgg
     5401 aactcgggca tcaaggaata aatcaataaa atcctgaggt tctcctggct caactccttt
     5461 tatctcatct ttagctcgct gatcaattct tgcctgtaca gctacttcta ttaaatcgaa
     5521 aattttgaag gctggaatga aacttgctgg tagcattttt gacatttttc tgaaaactcc
     5581 tgcgagcgga ggaaagattc cagtgataag gaacacatgc caggaaccgt tgaaaatctg
     5641 aaaacacttg ctattcttct tcagaatatt ataaacatac ggcttgaact tttggcagca
     5701 atgggttctt gaacatgttc gattcagact ggccaagagc aatgcgacta attactccca
     5761 gagtgtactc ttgatagact ctgaattttt atcataaaac ttaatcactt acaaaattac
     5821 cactcacatt aacatatcaa tatcttcgtt cttcctcact ttctgctcaa tattccataa
     5881 aatttcagtt ccacattctt gaacagagtt cctcactttt cgaagactgt tattggaaaa
     5941 agatggactt gacagtgtcc gaagacgttt ccaacgtttt ccttgagtcg agaaaatatt
     6001 gactcgtttg tctttttcag gatcaccaat gattggacgc aactttcgac catagaaatt
     6061 gtcgaattgt tttttgaata tttcat   
//

plain sequence
 
