# File: cyp-13A5_Deletion.ape

## Comments

>T10B9.2 unspliced + UTR + 2000 upstream + 2000 downstream; -ive strand; Sequence: 6086 bp
>gene: T10B9.2; chr: II; strain: VH7206; allele: hd7206; deletion size: 1318 bp; type: Homozygous; QC: PPPP; outcrossed: 0
>Deletion size = 1318 bp
>cyp-13A5 sgRNA #1, Chr II, TTAGAAGCAAGCACATGAGC; cut=9798882; strand=+1; GC=35; 3'GG=no; folding=-0.4
>cyp-13A5 sgRNA #2, Chr II, TCTCCCAATTTCAACAGTCC; cut=9800180; strand=+1; GC=45; 3'GG=no; folding=0.0

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
cyp-13A4_Unspliced_UTR | gene | forward | 1..1464
cyp-13A5 SV F | primer_bind | forward | 1698..1719
cyp-13A5 Upstream Homology Arm | misc_feature | forward | 2139..2588
Left Deletion Flank | misc_structure | forward | 2559..2588
Selection Cassette | variation | forward | 2589..8005
LoxP | misc_recomb | forward | 2610..2643
Pmyo-2 | regulatory:promoter | forward | 2686..3664
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 3191..3214
GFP(S65C) | CDS | forward | 3692..3862,3914..4067,4119..4282,4334..4561
unc-54 3'UTR | 3'UTR | forward | 4698..5432
Prps-27 | regulatory:promoter | forward | 5631..6424
neoR | CDS | forward | 6425..7219
NeoR SEC Insertion Primer 2 | primer_bind | forward | 6726..6747
unc-54 3'UTR | 3'UTR | forward | 7220..7918
LoxP | misc_recomb | forward | 7949..7982
Right Deletion Flank | misc_structure | forward | 8006..8035
cyp-13A5 Downstream Homology Arm | misc_feature | forward | 8006..8455
cyp-13A6_Unspliced_UTR | gene | forward | 8581..10185
cyp-13A5 SV R | primer_bind | reverse | 8741..8762

## Sequence

t agttggaaaaaatgcttgattttgctatttagagtatggtgtttatatacagatcagtttgtgtactgtggttcagtattgtctgctttcacacatttgtgtttcgcgtcgcaacaattatggtttgtgttttagaaggaaacatggatgatagcagagagcacgacaatttctgaacgtaggttgataaattggaaaaattatcaactcctatgcattgttggaaactgtta tgttgtgtcgacatcatacggg caggattccgtacttgaagttagttttgaccatacaaacgaactcttatttccacttatctctctaatatgcattctattttctgttagttatttttttgaaatgatgttttaaaatatgttttctaatttaaatgatttcctaacgtgtagatacattaggacgagtaaagtaattttttattagaaaagtgatttaaaattagaagcaagcacatgagctggttttcaatgttaataaattgtctttcttgaaggattctgcaattttttaaattagctatcgatttgaacaatttattaatcaaaaaaccaataggtacacatatgcataatttattatacaactaaaattaggaaTTAATTGGAATGTCTTGGTGTCAACTTGAGCATAACACTTCTTGGTGCAGTTGTGGCGGA TCCGACCAATTTTAATGGAATTTCTGTTTGCGTAGAAGTTTCGAATGTGTACTTTTTCAGGATGTGGGTCAAAAGCATTTTTTTTTCCATCATTGCCAATCGCATTCCAATACAAATTCTTGGTCCCATACCGAATGGAAGATATCCTCCTTTTGCATAAAATAGTTCGTCTCCAGATTCCCATctgcaaaaagtgatgttctttgggaagaaaaaattaaacttgaagctcatagctcatcttattaagaacatagaacatacCTCTCTGGCCTAAACTCATTTGCATCCTCTCCCCAAACTTTTGGATCATAATGAAGTGTCCATGTATCCACTTGAACATTTGTTCCTTTCTCTATTTGAACTCCAAGTACATTCGTTTCCTTCATGCACTTTCGGTTGTGAACGctgaaaatcgaa ttgacctgcttagattattaatagaatact ccgcggcgcatcaggcggcct ataacttcgtatagcatacattatacgaagttat tacgtagagctcggtacctcgtacgtaatacgactcactagt acaagtttgtacaaaaaagcaggctta ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGG gtaagtttaaacatatatatactaactaaccctgattatttaaattttcag CCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC gtaagtttaaacagttcggtactaactaaccatacatatttaaattttcag GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTT gtaagtttaaacatgattttactaactaactaatctgatttaaattttcag AACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG cattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcag agatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaac ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG ATCTCCTGTCATCTCACCTTGC TCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA ctcgaggtcgacaggcctgcatgcgtcgac ataacttcgtatagcatacattatacgaagttat agttgcaggaccactgcggccgc TGGATTTTCATAGTCTTGGAACTCGTGAAT CACTCCAAAAAATGGAAATCCACGGGGTCCTTTGACTCCTTTCCTGATCCAAAATGACCAAATCCAGATATAATATGTCAAAAGTCCAATAAACGAAGCACCAGCAATAAGTATACTTAAACTCATattttggaacaaaatatagtgtttcttctatctagtccagagagtatttatatactaaccgacatatttaccaaggccgtggtcacaaaaatcatttgacttgcttactcactcgggtgtttctcggcgcaacaagtcactatgatttgtgtttgaaaaatgggaaatgaatgacggaaaatttgtgacttggaattggacagaagtgaaacaagatagtgtttagatgatgattgattagctaggtaattcatttatgatatttattgttagagttttttgaagttgttcaag tagaataatgttctatactccaaagacaacaacattttttttttcatttttttgtaaaccatttttttgtaaactatcagtttgtttaactgttttcaatattaaatgttcaatataaaattatg tttgattttattttgatttatttatggctatttttcaattaaaacatatcagtaaaataaatattaattagacaatttttatacatgtaaaattacaaaaaattatgaatctctcggctccaatttcacaattacactttccggcgaaagtgtcaacttt cctaccaacttcaccggaatct