File: srpa-68_Wildtype.ape

Comments

>F55C5.8.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 6737 bp
>Deletion size = 2300 bp
>srpa-68 sgRNA #1, Chr V, GAAGAACAACAAGGACTTAC; cut=12289364; strand=-1; GC=40; 3'GG=no; folding=-0.0
>srpa-68 sgRNA #2, Chr V, CAGGGAAATGACAAACGACG; cut=12291644; strand=-1; GC=50; 3'GG=no; folding=-0.0

Features

Name Type Orientation Position
rskd-1_Unspliced_UTR gene reverse 1..1694
srpa-68-1 SV F primer_bind forward 1129..1149
srpa-68 Upstream Homology Arm misc_feature forward 1991..2440
Left Deletion Flank misc_structure forward 2411..2440
srpa-68 sgRNA #1 protein_bind reverse 2441..2460
srpa-68-1 WT F primer_bind forward 4199..4220
srpa-68 Downstream Homology Arm misc_feature forward 4741..5190
Right Deletion Flank misc_structure forward 4741..4770
srh-16_Unspliced_UTR gene forward 4940..6737
srpa-68-1 SV R primer_bind reverse 5311..5332

Sequence

gcagctctttgagccgttgccactagctgcccagccgtaactaagtagtcagctggagtctcttcgatttctgaatttatatatttttgaattcgccaaaaataagtgttctaccaatattatgatgcgtagatcctcgtaatcttgggaagagtcgtttcattgagcttttccttgcagttcgttgctcttcgtttgaagtttcggaagctatagccacgtccggaaattgaaagtcgtcgccagtctgaaaaatgagtggatatttcaaaaactattttactatttaattgaaaaatattttttatttctgattagatttttataatacattagacactacaaagaaaaagttcagtgaatacatggatatgacagaacttattgaggtgaaactgaagtgaacgaatcttttgagcgaaaataataatttagcaagtgggactgacttgttcgtgtgtcggttccaagagagcagcgtggttcggtgaatcggaatcactggaaataaattattttatagaaatgtgtacacagagatactaacctggaatttgtattgggttggtccaaaattgaagaagaattgtcataaaacacctcgtttccagtgatttcgtgtgccgtataggttttttctttggctttctgaaatgataaatatgttgttgagtggaaggaaaagtttcaaaatggtcaagagagtttcgatgaaattcggtttgtactcgtgccagtctgttccctggagactactatgggagtcttcgaaaatatggaaatattcgagaagttttcagttgttttgttttaattttcgagaacattttctcaaaagtaatacaaaaaactgaaacaaatcgtaaaattgacgagcatcgcgtcaacaagaaacaaacaagggaccaatttctttgtcaaaaagagaaaatgtgctagtgacatcatctgacgagacccttttcttcagtgtgtgagcatgactgtctgcgtcttatctgactctttttcgtatgtaaattctctcggcgtctcgatgctttagcattcagaaagaacgttacctcgacaaattcgtgtagaagacgtgatttacgtagaacttcgctgtcgaacacaaagtttaggcaggcttctatagcatgtcggcgctcttggatgacggatggatccgctgaaaaattagaagaaaaaataattgaagaataggattaaaaattatttcgagggaaaaagtgacacaaagtttagtatttaaggaaattaccaaaaacctacaagatccgatcaactttcccggtggaaaaacagggaaagttcctcgaagatacagctgtttatggagtttcgagattgccgcatgaagttttgtcatttctttatatcgagtgctcaatacaaaagttcgacgagaaacgtcatttgcgacgagatgggatgccactggattacattcaatttcgacagtgtaaactgtatgcgaaccagtaagatgtttctcttcggaaattgatgttactgtagagctgcatgaccagtcagtctgaaaattagcttattggcaagttggaaaaaacttctagaagtatctataggaataagcttttattaaccttaagttggtaaagtatccttcctatatcacttgtgcagtagctttcatctggatataacgagctcatctttcatactttcctgaaatgattgaaacttaaaacttcagaagttgtaattattaacaaaaaatcaacattcagggccaaaatagaattctacacatttagaacttttcaaaagttggaaaaactggcagataggaaaataaaatcgttcgggtttgtttacaattccgtgtcatttcaatcatgttgtctgcgtctcctgattttgtgaaatgaccaacaagatcgatgaggttgcgaaagaaaagaagatcataaaatatttttgcaaaggtttttatattttctcacctttatagaaaaatgggaaacttgaattcacaataaatatctttattaaaacgagactctgattccgaatagtacgaaacgccgttccaataaaaaacgaagtttccaagaagagatgagggcaggggaaaggtgtggatgaaggtatcaaataaataccttagctatttttgaatcaaaccataaaaaaagagcaaaaagtctggggtagaaatgaatatggtatcacaagaaatttagagaaatgttactttgtaacaaaatctcagaaaattgaagacaggaaagaacagaagaactgtgtgaaaaaccagaaaaaaaacattaaatatgagaaactgggccgatgcaagaggtatctatggacaacgaaacaacagtaacaaatgataattaatactctattcaacgtcttcgtagtcTTATTTATTTCCCCAGAAACTCTTTTTCCACCCGGAAAGCATTCCAGTAAGTCCTTGTTGTTCTTCTTCTTCTTGATTTGAAGTTTTTGACGAAGCAGCTGCTGCAGAAGATCCTTTCGCTGCCTTCTTTGGAGTAGCATCACGATCTTTTTGGAGTTTTTCAAGACGGTCATCAACATTGGGCATctgtaaataattgtttaaaactaaatacttttttgatgaataagtttagataaaattttgatcagcaagttcaaaaatatgataaatataaattacagcatagcatcaaacatgttttctctttgtttttaatggaaaacaacgattagtgtttttttttcaagtgaatacgaattgtcttactcttgcaaaagtcgaaaaattggaatttctaaactacaaaacgattttttaatttatataccaagttttgcgtgatgaaaaaacttacGGTAAGATGGAAGTTTGCCAAATCGAAGAAAATAGGTTTGTTCGGCATTGGAATAAAAGCTGGTGGTAAACTGGCAACTGGAATTGTCTTTTTATCTTTCAAGGAGTTACGAACATCCCATTGACGCCATTCATTGACTGTCTCCAGAAGTGGGCGTTTATCAATAATCTTGGCAAGTTCCGAATCGGTTTTAACATCTCCAGCAGCGGAAGCCAAACGAGCAGCTCGTACAGTAACCTTCGCAGATTCCACTTCAGATCGGAGCTCATTCAATGACGATTGAGTTTCATTGGTGATAAATGAGCTCGATTTTAATTTCTTAAGTTTTCCTTCTGCATCTTGAACTCGAGAAACAGTTCTGTCAAAGAGAGCAGCGGCTTCACTGTACTTATGAAGAGCAGAATAAGAAGAAGCCATATAGAAACATCTGAAAGCTCTGTAGTACTCAACTTTGACTTCAAATGCTTGAATAAGATTTTTGTCATGATCAGCTCCCGGGATTTCTGCGACTTCTTTGTAAATTTCGATAACAGAATCGTAGAGTCTCAAAAGATCTTGAGGTTTGCTCTTCTTTTCACTTTTTGTGTTATCAATAATAGCCAAGTAACGCGAAGCAGTTCCGTTCATTTTCAGGAATTCCAAATAAGCCTTGATTGATTGAAGAACAGTCGTGTCAGCATTTTCAGAACTTTTACGGCGAATAATATCCGAAATTCGATCTATAGCATCACGAGTATCAGCGGTGGCTTTTTCAAAGAGAGCCATTTTCTCTTTTGGAGTTTGGCATTGTGCCAATTCGACTTCTGTTTGCTTCCATTCTTGAACAACTTGCTTTGCTTTTTCATCGTCAACAGTACTCTTAGCTCCTCCCCATTCGATGGTGACAACTTCAGCGGATGTAGCAGAAGCTCGCATTTCCGAGATTAGTTTctgaaaacatatttatttgttaattttttttcttaatacttacATCAACAGAACTGTCTCCTCCTTCTCCCATTTGCATTCTCAATTCCATAAGCTCTGTCATTGTTCCAACAGCACCAGGAGACTCGGCGATATTGAATTCACAAAGTCGAAGTTGAGGTTGAATTTCACGACAACGACCCTTGAAAATCGAAGAAAGTGTAGTGTTGTTGGTGGCTTCAGCAAGCTTCTCATAAACAGTTTTAGCGAGCTTGAGGGATTCAGATGCTTTCTGCCAGTTTCGAGATTCAAAAGAGCACATTCCACGCATCCATGCTGCATAAGCTTGCGCTTCGAGTTTTGTTGGAGCGTCAAActgaaaataaagtgtatctggtcatcaaacaaagattttctctctttctttctgctcaaacttacTCTTTCGCTTTCCTGGACGATTTTTTCGAGATTTGTAGCGTGAAGAACAGCCTTACGGAGTGAATTTATCATTGAAAACTTCTTTCTTGACTTTTCTGGGTTGTCTTCAAGCGTGATCTTGTCAATCATTGCTTCAGCGTATCTCCGCTCTGATTCGAAAATTCCAAAATTAAGAAATTGCACATTTTGCACCGACTCCACAGAAAGCCACTTTTTGACAAACTTCGCTTTTCTCTTCTTCTGGCAATTGTGCGAATTCGTGAATTTCAATGCTTTTCTCATGCGCTCCAACTTTGCTGCACAGTACTTTctgaataaaatcggttatttaatgttttaatcctagcattgttacCTATAGCGAGCATAATCACCATGTCGTAAACCGTGTTGCTGTTGAGCGTCTTTTACGACctgaaaatatattgtttttattcaaaataagaagaaaaactattacCTGAAGAATATGAACAGTGGGGAATGGTGGAAGCTCTTCAGTCTCTGATTTCATCTCCACGTCGTTTGTCATttccctgaaaatattgaatttaaatatttaaatgttaattttacataaagaactaagtttctttgatgaaaaaataaaaaagaacggaaatattggacacatcggcagaaagcaaaaactgcgtcagcacagaagcgcgccagtgaccgcgctgcttgcgcactctccgatgaaatttgcggtttttcaaataaatataactgcaagaaaatccaaaattaccgtactttattttacgttgtatttaatgtttttttgttttccaataatgataagaacagagatgagcacagaaatctatacttttcgtttgaaaattacgttagataatctattataaataattcaattgtaatttaaatggcaaatcaattggtaattgatcaggttctcattcatacaaaattttgctcaatatttcagaaaacatcgatttagtttgatcatttggactaatctgattctgaaagttataatttattttatacacctcctcgtgctctgtaatatgtcatgtttgagtattgaattcattttctaacaacttttttctaaaaacatgcaggaaatgcattgtcaaacgccaccgatttcagtatataagttagtttttcataatgaaaagttaaaacctcattttaatttcagaacactcatgcatactctccatattctcgacgtaccactttatctgattgtgataatcgcccttttcaaaattaaatcaaatatttttcaaacgtataaatactatttgatatggcatacaataatgtgatctaacttctgaaaaggttttcaaaattttgtaacattttttagaaatgtgctcaccgagtttcatagctgctttctcatgcttccagttgttcatctaccgtacccattactacgtgcaactggaattctcgcagaatttggatttagtggcttattcttgttttatgtcctatctgcatttattatacgtgagttgaatattagtttaaaattaatcgatcgaaaaacacttttctatgtttcagaaacttcattttcaattatcgagatgttctatttccgatacaaagctagcgtttttgactatcaaagccgaaacttcacaaaattcgtaaagctattcatatattcatttcgtgttttaacgattctctttcctggaatcacattcggaacatttttctatagtattgaacgacaagaagaatacaaaaaagagcttgcaatggtagcgtatcaaaaatatttctagatgtgaaagattttaataaaaagtaatagaaacaacgctcgaattatcaaattaaataaaagaataagacataaagattagtgaattctaaatgcttttacaaaatttagcaatcgatccttaaacggcgcgcttttttgctgattctttttgcatttagcattcgaaatattcatctatgtcccatttataaagctttaaataaaagttgaaccagttcgtacgcttgtaaactccgccagcctctgcggaacacaaaaagtccactgatcgattgataaattttgagagtaatttcaaacagtcaatgatctatgttaagattgatccatcaattcctgaagtaacatgttattcatcagtacttgctgctccgttttccgattccgtaatgatatctacaatgattacctggttggcatgtataatttgtgcattctcagtaattccaagcaccactatttatttgaattctcatttgcggagtacgaaaaatatgtctccaggagttgtaaggatgcagaaaatgcttttatcaagtttgatcgttcaggtatataacatgataattttatattaagaaaatttaattttcagacattcgttcacggattcatgttgggaatacctaatattctgttcgtttacacaatattttttggggctcatcttgaaggtataattttatcttaaaaccggcatttcatctattattattttcagttgcggcatacgcagcattcatgtgtctg