# File: srpa-68_Deletion.ape

## Comments

>F55C5.8.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 6737 bp
>gene: F55C5.8.1; chr: V; strain: VH7200; allele: hd7195; deletion size: 2300 bp; type: Heterozygous; QC: PPHP; outcrossed: 0
>Deletion size = 2300 bp
>srpa-68 sgRNA #1, Chr V, GAAGAACAACAAGGACTTAC; cut=12289364; strand=-1; GC=40; 3'GG=no; folding=-0.0
>srpa-68 sgRNA #2, Chr V, CAGGGAAATGACAAACGACG; cut=12291644; strand=-1; GC=50; 3'GG=no; folding=-0.0

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
rskd-1_Unspliced_UTR | gene | reverse | 1..1694
srpa-68-1 SV F | primer_bind | forward | 1129..1149
srpa-68 Upstream Homology Arm | misc_feature | forward | 1991..2440
Left Deletion Flank | misc_structure | forward | 2411..2440
Selection Cassette | variation | forward | 2441..7857
LoxP | misc_recomb | forward | 2462..2495
Pmyo-2 | regulatory:promoter | forward | 2538..3516
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 3043..3066
GFP(S65C) | CDS | forward | 3544..3714,3766..3919,3971..4134,4186..4413
unc-54 3'UTR | 3'UTR | forward | 4550..5284
Prps-27 | regulatory:promoter | forward | 5483..6276
neoR | CDS | forward | 6277..7071
NeoR SEC Insertion Primer 2 | primer_bind | forward | 6578..6599
unc-54 3'UTR | 3'UTR | forward | 7072..7770
LoxP | misc_recomb | forward | 7801..7834
Right Deletion Flank | misc_structure | forward | 7858..7887
srpa-68 Downstream Homology Arm | misc_feature | forward | 7858..8307
srh-16_Unspliced_UTR | gene | forward | 8057..9854
srpa-68-1 SV R | primer_bind | reverse | 8428..8449

## Sequence

g aggcttctatagcatgtcggc ctgaaatgattgaaacttaaaacttcagaagttgtaattattaacaaaaaatcaacattcagggccaaaatagaattctacacatttagaacttttcaaaagttggaaaaactggcagataggaaaataaaatcgttcgggtttgtttacaattccgtgtcatttcaatcatgttgtctgcgtctcctgattttgtgaaatgaccaacaagatcgatgaggttgcgaaagaaaagaagatcataaaatatttttgcaaaggtttttatattttctcacctttatagaaaaatggga aacttgaattcacaataaatatctttattaaaacgagactctgattccgaatagtacgaaacgccgttccaataaaaaacgaagtttccaagaagagatgagggcaggggaaaggtgtggatgaaggtatcaaataaataccttagctatttttgaatcaaaccataaaaaaagagcaaaaagtctggggtagaaatgaatatggtatcacaagaaatttagagaaatgttactttgtaacaaaatctcagaaaattgaagacaggaaagaacagaagaactgtgtgaaaaaccagaaaaaaaacattaaatatgagaaactgggccgatgcaagaggtatctatggacaacgaaacaacagtaacaaatgataattaatactctattcaacgtcttcgtagtcTTATTTATTTCCCCAG AAACTCTTTTTCCACCCGGAAAGCATTCCA ccgcggcgcatcaggcggcct ataacttcgtatagcatacattatacgaagttat tacgtagagctcggtacctcgtacgtaatacgactcactagt acaagtttgtacaaaaaagcaggctta ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGG gtaagtttaaacatatatatactaactaaccctgattatttaaattttcag CCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC gtaagtttaaacagttcggtactaactaaccatacatatttaaattttcag GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTT gtaagtttaaacatgattttactaactaactaatctgatttaaattttcag AACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG cattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcag agatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaac ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG ATCTCCTGTCATCTCACCTTGC TCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA ctcgaggtcgacaggcctgcatgcgtcgac ataacttcgtatagcatacattatacgaagttat agttgcaggaccactgcggccgc aaaatattgaatttaaatatttaaatgtta attttacataaagaactaagtttctttgatgaaaaaataaaaaagaacggaaatattggacacatcggcagaaagcaaaaactgcgtcagcacagaagcgcgccagtgaccgcgctgcttgcgcactctccgatgaaatttgcggtttttcaaataaatataactgcaa