File: cyp-14A1_Wildtype.ape

Comments
>K09A11.2.1 unspliced + UTR + 2000 upstream + 2000 downstream; -ive strand; Sequence: 6315 bp
>Deletion size = 1777 bp
>cyp-14A1 sgRNA #1, Chr X, GCAAAAACTAATGAACCAGC; cut=13265457; strand=+1; GC=40; 3'GG=no; folding=-0
>cyp-14A1 sgRNA #2, Chr X, GGTTTGTCCAGTGGCATAGG; cut=13267199; strand=-1; GC=55; 3'GG=yes; folding=-2



Features
K09A11.1_Unspliced_UTR	gene	forward	1..1549
 

cyp-14A1 SV F	primer_bind	forward	1180..1201
ACTCGATATCTCTCAACAGCCG

cyp-14A1 Upstream Homology Arm	misc_feature	forward	2000..2399
TATACATTGTTCATTTTTATTCAAAAACAAAGATCAGTAGTTTAAAATTGAGGAATAATATTACAAGTGTAAGGTTTAGCGGTTAAAACAACTCCCCAATCTGGAGTCAAGTCAATATTAGAAGTGGCAACAAACTCATACTTCTGAATTAGTGCACTGAAAATCAGGAATAGTTCCATTCTTGCAAGCCCTTCTCCAACACAGTTTCTTTTTCCAATACTAAAGGGGATCGTTTTTTCAAGAACAGCCTGAAATACAAGACACATTTTTTAAAATTCAATTTCCTAATTCATGCTCTACCTTATCAAACGTTTTCCCATCGGATTGTAAGTAGCGGCTTGGATTGAATGTTTCTGGATCTTCAAACACTGTGTCATTTCTCATCACTGACCACAATTGC

Left Deletion Flank	misc_structure	forward	2370..2399
GTGTCATTTCTCATCACTGACCACAATTGC

cyp-14A1 sgRNA #1	protein_bind	forward	2400..2419
GCAAAAACTAATGAACCAGC

cyp-14A1 WT F	primer_bind	forward	3384..3404
ACCATCCGAGGCAAGAATACC

cyp-14A1 sgRNA #2	protein_bind	reverse	4157..4176
CCTATGCCACTGGACAAACC

cyp-14A1 Downstream Homology Arm	misc_feature	forward	4177..4626
TGGTGAATATTACCAATGAATGGAAGAGGTAGTGGTCCTTTTGGATATTTACTCACTTTCCAGTAAAAATGAAAAACATAAAAAATAATAAAAATTACAAAGGCTAAAATGAAGACTGACATTTTAACCTCTCAAATCTATTTCCCAATTGTCCCAAATAACTTCTCCCTGATTGTTTTATAAGGCCGTTCTAATATGATAACAATTGCGTCATTGTTGACAGACCAACCTTCACATTCAAAAGACACAATATGGTTTGTGAATGAGGGAATATTTGAACTAGACTGTCGGGAAAATGAAACAATGAACTTGAATTTCTCAATGTATGAATCTCAAGAAATGAATAATAATTCAATCGTTTATTTACAGCGTTCAAGTGAAGTTGGGTTTAAGCAGCCTGAGGAACTAGCTCGCATATGTATGGCTTTGTGGTGAGGACTGCTCCGCAGA

Right Deletion Flank	misc_structure	forward	4177..4206
TGGTGAATATTACCAATGAATGGAAGAGGT

cyp-14A2_Unspliced_UTR	gene	reverse	4522..6315
 

cyp-14A1 SV R	primer_bind	reverse	5218..5239
CCAGCGGAGAGAGTTAGAAGTG


formatted sequence

        1 tgagcacgtt gttctctctg ttgacatttg gtccgacaaa ggttcgatac aggagatgat
       61 tggagttact ggaaactttt tgaaaaacga gaaagtagtt cgtcgtacaa ttggagttat
      121 caactatcaa aaggacgtac acaacggagt ctatgttgct gctgaacttg ataacctgat
      181 ggcccggttc aatttaaatg gaaaagtaga agttgtctgc agagattaca attcaattgt
      241 agaaaatgca tgtgaccatt tgaaaatttc ttcagttaat tgcttcaatc aacagctaca
      301 tttggcagct tgtgaggcaa aatcaaaaat tggacaaata aacagagttt tgttgaactg
      361 caagctactt gtcaaaaatt tgaaaaacag ttctgtcacc tggcaaacat cagagaaaat
      421 ccaaagttta atggaaattc caaatttgtg cctcaatcat tgctcagacg aatgttggtg
      481 tttcttgcaa gaattctttg ggagggtcat agaatgccaa caagtgattg aagaacttca
      541 aaaactgcaa tcgagcgatt gggaccttat gagagatgtc gtggaaattc tggcaccact
      601 tagcaagttt tctaaattat cagaggtaaa tttgctacta tcatcattac ccctctatgt
      661 tttttttcca gtcaaaagag tcgtctgctt cagtgatcat tccatactgt gattatcttg
      721 tgaaagcata cgaatcaaca caaaatcagc cggaagcatt tttggcagtt aaagaacaca
      781 tggaaagaga gctgcacaaa tacacatctt gccagtactt gaaattttcc actctacttg
      841 atccgcgata taaggtataa ctttaaaata tgagaacaag atttgttaag ttgtatattt
      901 caggatttat atacaaacga cagtgacact tccaatttga taaaggccat tgaaactgaa
      961 tttgaaacga tggattacaa aaacagaaca tttgaagaga tttcttcaga attgtgtgga
     1021 ccagctccaa aatctcagaa acttgatttt gacgcttata tacaggtatt taaaactcaa
     1081 aacatagtta agttaacaaa taaattgcag gaaaaaatta aatctcgcgg caaacataac
     1141 gcattaactg gtgaaaaatt gaccgtgaga atggaagtga ctcgatatct ctcaacagcc
     1201 gttgaagatg taacgcctag agatttctgg ctaaacgatg tgacaaaagc ttcttatcca
     1261 agacttgcca aattcgctcg acgttttata atttgtccga cgggatctag tgcagccgag
     1321 aaactatttt caacagatcg agcgactatg gatgagtaca gaaatacttt gtctgatgaa
     1381 aatttgaaaa tgctcatttt tttgaacaaa aatatttctt caatgactga tcttgaattt
     1441 gaaacagagc gggacctaca tgactaatta ttgatctaat gattttattt atttgttact
     1501 gaaaatttct gatttttttt tgttttccaa taaaaatctt tatacttttt gttgcaaatt
     1561 ttgtgtcttt aaacgatagg gcaataaaaa ctagcgttgg aaaataccat gaaaaaataa
     1621 atcgaaaaaa tcaaaaaaga aatccgtcga accggattcg aaccagtgac ctaaggatac
     1681 ccgctgactc tacagtcctc cgctctacca actgagctat cgacggcatg tgaacatgca
     1741 acacgctctg cgtaaaagtt cttgcccctt ctcccactct ctctctctct ctctcttttg
     1801 ctttcgtgcg gagaagcatg aaagatagag cggagagaag aagcgtgaaa agtgccggcg
     1861 gctttcatgt gcttgctgac tagaaaagag cagtggctat tttctagtta gataaattga
     1921 atattgtgaa aatggtttaa taaatgcacc actgtacaag aaaaagctta ttgaaattca
     1981 tctttaaaac tacatatatt atacattgtt catttttatt caaaaacaaa gatcagtagt
     2041 TTAAAATTGA GGAATAATAT TACAAGTGTA AGGTTTAGCG GTTAAAACAA CTCCCCAATC
     2101 TGGAGTCAAG TCAATATTAG AAGTGGCAAC AAACTCATAC TTCTGAATTA GTGCACTGAA
     2161 AATCAGGAAT AGTTCCATTC TTGCAAGCCC TTCTCCAACA CAGTTTCTTT TTCCAATACT
     2221 AAAGGGGATC GTTTTTTCAA GAACAGCctg aaatacaaga cacatttttt aaaattcaat
     2281 ttcctaattc atgctctacC TTATCAAACG TTTTCCCATC GGATTGTAAG TAGCGGCTTG
     2341 GATTGAATGT TTCTGGATCT TCAAACACTG TGTCATTTCT CATCACTGAC CACAATTGCG
     2401 CAAAAACTAA TGAACCAGCT GGAACTCTGT TTCCGTGAAT TTCAGTTTCC TCTTTGCctg
     2461 aaaatgcaca agttgacttt gaaaaaaaaa acagtatcca aaaatgaaca tcttacACTG
     2521 ATGAGACCCC AAGAAAGGAA TCATGTTAGA ACATCGCTGC ACTTCATGGA TCACTGCTTG
     2581 AGTATATGGC ATATTTGGTT TATCTGACAT TGATGGCAAA CGTGCTGTTC CAACATTGTC
     2641 CAGTATTTCT TTTCTGACTT TATCTTGAAC TTCTGGATAT TTCATCATAA ATGCCAAATG
     2701 CCAGCGAAGA GAATTTGATG TGGTCTCCAT GCCAGCTAAC CAAAAATCGA GAACTGTTGC
     2761 ACACAAGTTT GTAATActga aatagtgatt atatttagtt ttagagaata agcaaataca
     2821 aacTCAAGTC CAGGATTTCC CGTTTGCTTC ATCTGTTGCA TGTAAGCGTG GACAAAATTT
     2881 TCAGGTTCAC TCTCCCCATC GTATTTTTTC ACTTGCGTAG CTACTTCTTC TTCGATAAAT
     2941 GTATGGTAct gaaaatctca ctcaatttct acttaagtaa taactaatat aattcaactc
     3001 acCGAAGATA TATTATTTTT AATGTTGTGA TATCCGATAT CTCCAACTAT CGGAACATGT
     3061 TTCAACCATG GAAACGCAGA AACCAATAGT GGCATTTTTC CTTGAAGATG CCTCAGATGT
     3121 TTATCGACAA TTTTAACAAA CGTCTTGAAT TTCTCCGAGT CTTCATATTT GTAATGAAAA
     3181 CCGAACAGTG TTTCATTGAT CACGTTTCCC ACACAAAGTT GAATTGGCCA AAAAACGTCG
     3241 ACGTTTTTCT TATCATTAAT GTTTTCCAGT TGAATAAGCA TTTCGTGAAC CGAACGGATT
     3301 ACTTGTTCTT CCATCAAATT TCTCCCTAAA CCAAAGTCAC GAAGGATTTT CAAGGAAGTT
     3361 CTTCTCTGAA GTCGCCAGTG GTCACCATCC GAGGCAAGAA TACCAGTGTG ATCGTGAACT
     3421 GGCAGAAGGG TTTCCGGAGA ACGGTGAGCT CGGTAAGTGA ATGTGTCACc tgaagaactt
     3481 atacagttat acttaaagcg tttaaataat tgatggaata ttggaaaagt ttgtagtttt
     3541 taatgggtta accggtataa agtaaagctt tctacgaaaa atctgtcagt tttgcaaagc
     3601 ttgaaaaata gtcttgcagc ctcttatagt cttgtaactt ctatcagtct tgcaccccta
     3661 cggaccgatt ggaaattagt tttgtttgca atacaaatag agtaaatact gtaaattata
     3721 ccacaattaa acagttgata atttacaact tttttactac tccctatgct gattcaaagt
     3781 ttattaacag aaaccccaat ttttttgaaa ataaactgct acaaacaatg tgtctctaga
     3841 gatatgtttt tgtaacttga ttttttttgt tggaaaatga tcaagttttt gtatttttta
     3901 aaatattttt aattatattc tataatgttt cataaattta tattattgaa acgttgagat
     3961 ttttattttt aaattttatt taaaacacac CTTGATTAAC AAATGCATCT TTTATATGCT
     4021 CATAATCAGT CAACACAATT GCCGGAAATG GAATCCATAT GGTAAAACAT GGCCCATAGG
     4081 TTTTAGATAA AACGTCAAAA TACTTTTGAA CATTGTCTGG AGGGAActga aatattttat
     4141 ttagaacaac tttcccccta tgccactgga caaacCTGGT GAATATTACC AATGAATGGA
     4201 AGAGGTAGTG GTCCTTTTGG ATATTTACTC ACTTTCCAGT AAAAATGAAA AACATAAAAA
     4261 ATAATAAAAA TTACAAAGGC TAAAATGAAG ACTGACATtt taacctctca aatctatttc
     4321 ccaattgtcc caaataactt ctccctgatt gttttataag gccgttctaa tatgataaca
     4381 attgcgtcat tgttgacaga ccaaccttca cattcaaaag acacaatatg gtttgtgaat
     4441 gagggaatat ttgaactaga ctgtcgggaa aatgaaacaa tgaacttgaa tttctcaatg
     4501 tatgaatctc aagaaatgaa taataattca atcgtttatt tacagcgttc aagtgaagtt
     4561 gggtttaagc agcctgagga actagctcgc atatgtatgg ctttgtggtg aggactgctc
     4621 cgcagaccgg tttgacatca atattagttt ttggaatgaa ctcgtatttt tggatcaatg
     4681 cactaaaaat caagaagagc tccatgcggg ccaatccttc tccaacgcaa tttcgctttc
     4741 cgacactgaa gggaattgtt cgttctaata cagactgaaa taaaatttta attgaacgaa
     4801 atacttcaac tattgattgt attttacctt atttattgtt tttccatcgg ccattaaata
     4861 tctatcaggg ttgaatttag agctctcttc gaaaactggg tcatttttca gaactgacca
     4921 aatttgtgca aatacaaggg taccagttgg tattttcttc cctttaagta gaatgtcatc
     4981 tcggtctgta acaaattgat catactgaat acaacaattg tacacaaagt tacttgtatg
     5041 agtcccaaga attggtatca tgtttgagca tctttgaact tcatggatga ctgcttgagt
     5101 gtacggcata tttggtttgt ctgacattga tggtaatcta gcagttccta cattatcaag
     5161 tatttccttc cggactttat cttgaatttc tggatatttc atcatgaatg ccaaatgcca
     5221 gcggagagag ttagaagtgg tttccattcc agccagccag aaatcgataa ctgatgcaca
     5281 taggttgttc atgctgaaat tgaaaaatgt atcaaatgga tacgaacatt gaaaaaaaag
     5341 taaacaattt gggatggaac gagaattcga ctcactcaag atttggattt ccactttgtt
     5401 tcatttgttg catgtatgca tgcacaaagt tttctggttc gctttctcca tcataatgtt
     5461 tgatctggct ggtcacttca tcattaataa attcttgata ctgaaaataa aattattccc
     5521 attttttcga cttatctgtt tttaaaattt accgactgaa tgttcttttt gattctgtga
     5581 tatcctagct ctccaattac tgggaaatgt tttaaccatg ggaaagctga catgagaaag
     5641 atcgtctttc cttgtacggt tttcaagtgc ttatctagaa ctttcacaaa tgtctcaaac
     5701 ttcttagaat cctcatattt gtaatggtaa ctgaagaggc tctcgttgat gacatttcca
     5761 acacaaagtt gaatcggcca aaacatgtca acattcttct tatcagcaat acgctcaagt
     5821 tgtgcaagca tttcgtggac tgatctcatg acttgttctt ccatcaaatt tctgcctaaa
     5881 ccaaagtcgc gaagaatttt caaagcagtt cttctctgaa atctccaatt gtctccattc
     5941 gaaaacaaga ctcctgtgtt caggtgaggc tggagcaaag tttctggtgg tcgatcagca
     6001 cggtaaataa aggcgtcacc tgtaaatcgg ttaagttcat gaaaatgtct agaaaacaaa
     6061 agaaaattga tcaatgctta atttagggaa ttcagcatcg gaaaaaatcc cgtgcaaatt
     6121 taaagttgga cttttttatt gaggtgcata ttttgaatat aacagaagaa ttcgatggat
     6181 ataaatttaa taatatttct aatattcaaa tgattaacat tttgccctgc taaaaattgt
     6241 tgtcacttga aaatgcagca taccagtgtg taaaaaacac aaaacgtcat actgtttata
     6301 attgcattat aacta    
//

plain sequence
 
