# File: cyp-14A1_Deletion.ape

## Comments

>K09A11.2.1 unspliced + UTR + 2000 upstream + 2000 downstream; -ive strand; Sequence: 6315 bp
>gene: K09A11.2.1; chr: X; strain: VH7199; allele: hd7199; deletion size: 1777 bp; type: Homozygous; QC: PPPP; outcrossed: 0
>Deletion size = 1777 bp
>cyp-14A1 sgRNA #1, Chr X, GCAAAAACTAATGAACCAGC; cut=13265457; strand=+1; GC=40; 3'GG=no; folding=-0
>cyp-14A1 sgRNA #2, Chr X, GGTTTGTCCAGTGGCATAGG; cut=13267199; strand=-1; GC=55; 3'GG=yes; folding=-2

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
K09A11.1_Unspliced_UTR | gene | forward | 1..1549
cyp-14A1 SV F | primer_bind | forward | 1180..1201
cyp-14A1 Upstream Homology Arm | misc_feature | forward | 2000..2399
Left Deletion Flank | misc_structure | forward | 2370..2399
Selection Cassette | variation | forward | 2400..7816
LoxP | misc_recomb | forward | 2421..2454
Pmyo-2 | regulatory:promoter | forward | 2497..3475
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 3002..3025
GFP(S65C) | CDS | forward | 3503..3673,3725..3878,3930..4093,4145..4372
unc-54 3'UTR | 3'UTR | forward | 4509..5243
Prps-27 | regulatory:promoter | forward | 5442..6235
neoR | CDS | forward | 6236..7030
NeoR SEC Insertion Primer 2 | primer_bind | forward | 6537..6558
unc-54 3'UTR | 3'UTR | forward | 7031..7729
LoxP | misc_recomb | forward | 7760..7793
cyp-14A1 Downstream Homology Arm | misc_feature | forward | 7817..8266
Right Deletion Flank | misc_structure | forward | 7817..7846
cyp-14A2_Unspliced_UTR | gene | reverse | 8162..9955
cyp-14A1 SV R | primer_bind | reverse | 8858..8879

## Sequence

a actcgatatctctcaacagccg ttgaagatgtaacgcctagagatttctggctaaacgatgtgacaaaagcttcttatccaagacttgccaaattcgctcgacgttttataatttgtccgacgggatctagtgcagccgagaaactattttcaacagatcgagcgactatggatgagtacagaaatactttgtctgatgaaaatttgaaaatgctcatttttttgaacaaaaatatttcttcaatgactgatcttgaatttgaaacagagcgggacctacatgactaattattgatctaatgattttatttatttgttactgaaaatttctgatttttttttgttttccaataaaaatctttatactttt tgttgcaaattttgtgtctttaaacgatagggcaataaaaactagcgttggaaaataccatgaaaaaataaatcgaaaaaatcaaaaaagaaatccgtcgaaccggattcgaaccagtgacctaaggatacccgctgactctacagtcctccgctctaccaactgagctatcgacggcatgtgaacatgcaacacgctctgcgtaaaagttcttgccccttctcccactctctctctctctctctcttttgctttcgtgcggagaagcatgaaagatagagcggagagaagaagcgtgaaaagtgccggcggctttcatgtgcttgctgactagaaaagagcagtggctattttctagttagataaattgaatattgtgaaaatggtttaataaatgcaccactgtacaagaaaaagcttattgaaattcatctttaaaactacatatat tatacattgttcatttttattcaaaaacaaagatcagtagtTTAAAATTGAGGAATAATATTACAAGTGTAAGGTTTAGCGGTTAAAACAACTCCCCAATCTGGAGTCAAGTCAATATTAGAAGTGGCAACAAACTCATACTTCTGAATTAGTGCACTGAAAATCAGGAATAGTTCCATTCTTGCAAGCCCTTCTCCAACACAGTTTCTTTTTCCAATACTAAAGGGGATCGTTTTTTCAAGAACAGCctgaaatacaagacacattttttaaaattcaatttcctaattcatgctctacCTTATCAAACGTTTTCCCATCGGATTGTAAGTAGCGGCTTGGATTGAATGTTTCTGGATCTTCAAACACT GTGTCATTTCTCATCACTGACCACAATTGC ccgcggcgcatcaggcggcct ataacttcgtatagcatacattatacgaagttat tacgtagagctcggtacctcgtacgtaatacgactcactagt acaagtttgtacaaaaaagcaggctta ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGG gtaagtttaaacatatatatactaactaaccctgattatttaaattttcag CCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC gtaagtttaaacagttcggtactaactaaccatacatatttaaattttcag GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTT gtaagtttaaacatgattttactaactaactaatctgatttaaattttcag AACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG cattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcag agatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaac ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG ATCTCCTGTCATCTCACCTTGC TCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA ctcgaggtcgacaggcctgcatgcgtcgac ataacttcgtatagcatacattatacgaagttat agttgcaggaccactgcggccgc TGGTGAATATTACCAATGAATGGAAGAGGT AGTGGTCCTTTTGGATATTTACTCACTTTCCAGTAAAAATGAAAAACATAAAAAATAATAAAAATTACAAAGGCTAAAATGAAGACTGACATtttaacctctcaaatctatttcccaattgtcccaaataacttctccctgattgttttataaggccgttctaatatgataacaattgcgtcattgttgacagaccaaccttcacattcaaaagacacaatatggtttgtgaatgagggaatatttgaactagactgtcgggaaaatgaaacaatgaacttgaatttctcaatgtatgaatctcaagaaatgaat