# File: cyp-14A1_Wildtype.ape

## Comments

>K09A11.2.1 unspliced + UTR + 2000 upstream + 2000 downstream; -ive strand; Sequence: 6315 bp
>Deletion size = 1777 bp
>cyp-14A1 sgRNA #1, Chr X, GCAAAAACTAATGAACCAGC; cut=13265457; strand=+1; GC=40; 3'GG=no; folding=-0
>cyp-14A1 sgRNA #2, Chr X, GGTTTGTCCAGTGGCATAGG; cut=13267199; strand=-1; GC=55; 3'GG=yes; folding=-2

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
K09A11.1_Unspliced_UTR | gene | forward | 1..1549
cyp-14A1 SV F | primer_bind | forward | 1180..1201
cyp-14A1 Upstream Homology Arm | misc_feature | forward | 2000..2399
Left Deletion Flank | misc_structure | forward | 2370..2399
cyp-14A1 sgRNA #1 | protein_bind | forward | 2400..2419
cyp-14A1 WT F | primer_bind | forward | 3384..3404
cyp-14A1 sgRNA #2 | protein_bind | reverse | 4157..4176
cyp-14A1 Downstream Homology Arm | misc_feature | forward | 4177..4626
Right Deletion Flank | misc_structure | forward | 4177..4206
cyp-14A2_Unspliced_UTR | gene | reverse | 4522..6315
cyp-14A1 SV R | primer_bind | reverse | 5218..5239

## Sequence

a actcgatatctctcaacagccg ttgaagatgtaacgcctagagatttctggctaaacgatgtgacaaaagcttcttatccaagacttgccaaattcgctcgacgttttataatttgtccgacgggatctagtgcagccgagaaactattttcaacagatcgagcgactatggatgagtacagaaatactttgtctgatgaaaatttgaaaatgctcatttttttgaacaaaaatatttcttcaatgactgatcttgaatttgaaacagagcgggacctacatgactaattattgatctaatgattttatttatttgttactgaaaatttctgatttttttttgttttccaataaaaatctttatactttt tgttgcaaattttgtgtctttaaacgatagggcaataaaaactagcgttggaaaataccatgaaaaaataaatcgaaaaaatcaaaaaagaaatccgtcgaaccggattcgaaccagtgacctaaggatacccgctgactctacagtcctccgctctaccaactgagctatcgacggcatgtgaacatgcaacacgctctgcgtaaaagttcttgccccttctcccactctctctctctctctctcttttgctttcgtgcggagaagcatgaaagatagagcggagagaagaagcgtgaaaagtgccggcggctttcatgtgcttgctgactagaaaagagcagtggctattttctagttagataaattgaatattgtgaaaatggtttaataaatgcaccactgtacaagaaaaagcttattgaaattcatctttaaaactacatatat tatacattgttcatttttattcaaaaacaaagatcagtagtTTAAAATTGAGGAATAATATTACAAGTGTAAGGTTTAGCGGTTAAAACAACTCCCCAATCTGGAGTCAAGTCAATATTAGAAGTGGCAACAAACTCATACTTCTGAATTAGTGCACTGAAAATCAGGAATAGTTCCATTCTTGCAAGCCCTTCTCCAACACAGTTTCTTTTTCCAATACTAAAGGGGATCGTTTTTTCAAGAACAGCctgaaatacaagacacattttttaaaattcaatttcctaattcatgctctacCTTATCAAACGTTTTCCCATCGGATTGTAAGTAGCGGCTTGGATTGAATGTTTCTGGATCTTCAAACACT GTGTCATTTCTCATCACTGACCACAATTGC GCAAAAACTAATGAACCAGC ACCATCCGAGGCAAGAATACC cctatgccactggacaaacC TGGTGAATATTACCAATGAATGGAAGAGGT AGTGGTCCTTTTGGATATTTACTCACTTTCCAGTAAAAATGAAAAACATAAAAAATAATAAAAATTACAAAGGCTAAAATGAAGACTGACATtttaacctctcaaatctatttcccaattgtcccaaataacttctccctgattgttttataaggccgttctaatatgataacaattgcgtcattgttgacagaccaaccttcacattcaaaagacacaatatggtttgtgaatgagggaatatttgaactagactgtcgggaaaatgaaacaatgaacttgaatttctcaatgtatgaatctcaagaaatgaat