# File: rnst-2_Deletion.ape

## Comments

>K10C9.3.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 10314 bp
>gene: K10C9.3.1; chr: V; strain: VH7194; allele: hd7194; deletion size: 4597 bp; type: Homozygous; QC: PPPP; outcrossed: 0
>Deletion size = 4597 bp
>rnst-2 sgRNA #1, Chr V, CTGGGAAGTGAGCAGTCTAC; cut=1060677; strand=-1; GC=55; 3'GG=no; folding=-2.1
>rnst-2 sgRNA #2, Chr V, AGCGTACCTTCTTATCCCGG; cut=1065254; strand=-1; GC=55; 3'GG=yes; folding=-0.0

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
rnst-2 SV F | primer_bind | forward | 251..272
rnst-2 Upstream Homology Arm | misc_feature | forward | 631..1080
Left Deletion Flank | misc_structure | forward | 1051..1080
Selection Cassette | variation | forward | 1081..6497
LoxP | misc_recomb | forward | 1102..1135
Pmyo-2 | regulatory:promoter | forward | 1178..2156
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 1683..1706
GFP(S65C) | CDS | forward | 2184..2354,2406..2559,2611..2774,2826..3053
unc-54 3'UTR | 3'UTR | forward | 3190..3924
Prps-27 | regulatory:promoter | forward | 4123..4916
neoR | CDS | forward | 4917..5711
NeoR SEC Insertion Primer 2 | primer_bind | forward | 5218..5239
unc-54 3'UTR | 3'UTR | forward | 5712..6410
LoxP | misc_recomb | forward | 6441..6474
rnst-2 Downstream Homology Arm | misc_feature | forward | 6498..6947
Right Deletion Flank | misc_structure | forward | 6498..6527
K10C9.7_Unspliced_UTR | gene | reverse | 6908..8794
rnst-2 SV R | primer_bind | reverse | 7675..7696

## Sequence

ttttcaaacctttgtaaatcaaagtggcctgaacattttactctagttctaattttctgaaatgctgctactttgtatttgttgacaattttaaattacctcgtttaaatatcagtcgtttcattacagcaacaagttttaaaaaacttgtaaaaatgtttgattttgtgctttttgtgattacactttggggggtatcatattaacttaacgtcgtaataatttttgtttttatgttaagatttacaaa gtaagctgacatgccttatgcc taaatatctacgttttttttaaataacgaagcagacatgcttttttctatatctctaaaaattacttttgcgccaaattaaaaatgtaattatgcacggttgaacttatctaaaaactgtatttcctctattagacttgcacccttacgagtcaatcaaaaattagtcttgcatgcaaaagtaatagaggcaatacggttgtttaatgttgtacaattatcttaaataactacctaattcaaattatttcatttaaagttttgataagttagggggtttcaagtaacttcaatcacttctgcagaggaaaatgtttgaaaacaataatttaaaacaagttcaaaatttgaaaatatca gtgtttatttcctgctttttaatgatgggtgaaaagtggctcaggttttttgacggaaaatttcacatcaacttttgaaatttctacattacatttgatcattttagtttgatattagccgctattcttggtggccgaactttttcgcaatttctaggccacgctgagtcgctatcaaactttctgttttcaggtcacactatctctgtttgttatcatttattgttttttctttacctactttcttgataagatatatgtatcattcttttttgcgtttcttctgcgaggtcaaatatgttctgttttcctaataaattagttttcaaaccaaaaaccatcctatgtagttgcacttaattaaaaattatctggtcttaaatctgaatggtctcaacaataaatcatgtggcagctgtt agcacctgtcacttgacactcctttgtcca ccgcggcgcatcaggcggcct ataacttcgtatagcatacattatacgaagttat tacgtagagctcggtacctcgtacgtaatacgactcactagt acaagtttgtacaaaaaagcaggctta ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGG gtaagtttaaacatatatatactaactaaccctgattatttaaattttcag CCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC gtaagtttaaacagttcggtactaactaaccatacatatttaaattttcag GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTT gtaagtttaaacatgattttactaactaactaatctgatttaaattttcag AACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG cattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcag agatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaac ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG ATCTCCTGTCATCTCACCTTGC TCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA ctcgaggtcgacaggcctgcatgcgtcgac ataacttcgtatagcatacattatacgaagttat agttgcaggaccactgcggccgc tttaagcctcaaaattgtcatgctaaaaaa ttaaatttcagACCAAACAGTTCCTTCTCGGTGACATTCGCTTGTGTCTCAACAAGGACTTGACAATCCGAGACTGTCCGACTGATGGAAAACATCCGAATCGAGTCAGCAGATTCGAGAGATCAATTGGCAGAAATAGACGCGGGCCACCACTTCCAAGCTTCCAGgttggttacgatttaaaattttgtttgaccttttccaaaattccgaatccttcattttttgttggaattttgcaatttaataaattgccgattcaagatttttcggcaattgacagttttcaaaactctcagaccaaatttttctcgagatactttcggattttttattcgaatttttccactgtcaaaacaaaaaattcggaacttttacat