# File: ZK632.4_Deletion.ape

## Comments

>ZK632.4.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 5940 bp
>gene: ZK632.4; chr: III; strain: VH7191; allele: hd7181; deletion size: 1382 bp; type: Heterozygous; QC: PPHP; outcrossed: 0
>Deletion size = 1382 bp
>ZK632.4 sgRNA #1, Chr III, TCACTACTATTTACTCCACG; cut=9806006; strand=-1; GC=40; 3'GG=no; folding=-0.0
>ZK632.4 sgRNA #2, Chr III, GGAAGTCTTGCATTAGATGG; cut=9807368; strand=-1; GC=45; 3'GG=yes; folding=-2.2

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
riok-3_Unspliced_UTR | gene | reverse | 1..1497
ZK632.4 SV F | primer_bind | forward | 937..958
ZK632.4 Upstream Homology Arm | misc_feature | forward | 2040..2489
Left Deletion Flank | misc_structure | forward | 2460..2489
Selection Cassette | variation | forward | 2490..7906
LoxP | misc_recomb | forward | 2511..2544
Pmyo-2 | regulatory:promoter | forward | 2587..3565
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 3092..3115
GFP(S65C) | CDS | forward | 3593..3763,3815..3968,4020..4183,4235..4462
unc-54 3'UTR | 3'UTR | forward | 4599..5333
Prps-27 | regulatory:promoter | forward | 5532..6325
neoR | CDS | forward | 6326..7120
NeoR SEC Insertion Primer 2 | primer_bind | forward | 6627..6648
unc-54 3'UTR | 3'UTR | forward | 7121..7819
LoxP | misc_recomb | forward | 7850..7883
ZK632.4 Downstream Homology Arm | misc_feature | forward | 7907..8356
Right Deletion Flank | misc_structure | forward | 7907..7936
ZK632.4 SV R | primer_bind | reverse | 8955..8976
gtap-2_Unspliced_UTR | gene | forward | 9065..9975

## Sequence

g ctcatcaagcttcgcatagagc aaaacagtgactagcacttttgtatgtaattgaaattccaaaattccaatttttagctgaaataacgtggaagctatagcatggcaagtttaatttttaaatacaatcattgcgtaaaactgtggtgaattatataaagacataaaaagatttctgaaTTAATCAATAATCAGACGCTTATTTGAAAGACTTTCGAGGGCTCTTGGTGATGGTGTGAACGCTCGGAATGCAAGAAAATCATCAGAAATATTAATCTTGGGACGTTCAGCGTCATGTGTAGCTCCAATGAAAACGACTTCTCCTCGATTCAATACTTGTGATGCATCTCCCAATGTAACTGTTGCAGTACCATAGAGAACCGTCAAGACAGAACATGATTCGGAGTAAGGAAGTTGGTAGATCGATCCAGCGGGTCCTTTG ACTTCCTGCACCCAGAACTCATCAATTCCA ccgcggcgcatcaggcggcct ataacttcgtatagcatacattatacgaagttat tacgtagagctcggtacctcgtacgtaatacgactcactagt acaagtttgtacaaaaaagcaggctta ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGG gtaagtttaaacatatatatactaactaaccctgattatttaaattttcag CCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC gtaagtttaaacagttcggtactaactaaccatacatatttaaattttcag GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTT gtaagtttaaacatgattttactaactaactaatctgatttaaattttcag AACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG cattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcag agatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaac ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG ATCTCCTGTCATCTCACCTTGC TCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA ctcgaggtcgacaggcctgcatgcgtcgac ataacttcgtatagcatacattatacgaagttat agttgcaggaccactgcggccgc AGCCATGCTAGAATTTCCTTTGGGTCCCCA TGCGTAGTTATTGACAGTGCACTTTAGCTTCAGAAGCATctgaaaacatgaattatggaagaaatgttttgagagaacagattatgttcgaaatatgagttagggagaaaataaaagatataatgcatcaaaatgacggtaaacgactgatctctctagttttctctgatcaacgcatgcgctaaaatcgacacgtgactaaatataatcagtcataagttgaccataagacaagagaaaaagacttccgaactggaagacacggaatacatatatatgaccgagaggctcggagacgcagattgaaaacgctgagatcagaactataaaacgagtgcgaccgtcgacttttttcatatcttgagtgggtatgcgtgtgagattgcgtcaacgtgactgcctaaactaattgtagagaaa ggcgtgctctactaaactcctc ctaatgtttttttgttgttttttcaattgccactgtttttttttctaatttcaataaaactgaatcctttttaaattgtttttttcag