# File: ZK632.4_Wildtype.ape

## Comments

>ZK632.4.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 5940 bp
>Deletion size = 1382 bp
>ZK632.4 sgRNA #1, Chr III, TCACTACTATTTACTCCACG; cut=9806006; strand=-1; GC=40; 3'GG=no; folding=-0.0
>ZK632.4 sgRNA #2, Chr III, GGAAGTCTTGCATTAGATGG; cut=9807368; strand=-1; GC=45; 3'GG=yes; folding=-2.2

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
riok-3_Unspliced_UTR | gene | reverse | 1..1497
ZK632.4 SV F | primer_bind | forward | 937..958
ZK632.4 Upstream Homology Arm | misc_feature | forward | 2040..2489
Left Deletion Flank | misc_structure | forward | 2460..2489
ZK632.4 sgRNA #1 | protein_bind | reverse | 2490..2509
ZK632.4 WT F | primer_bind | forward | 2955..2976
ZK632.4 sgRNA #2 | protein_bind | reverse | 3852..3871
ZK632.4 Downstream Homology Arm | misc_feature | forward | 3872..4321
Right Deletion Flank | misc_structure | forward | 3872..3901
ZK632.4 SV R | primer_bind | reverse | 4920..4941
gtap-2_Unspliced_UTR | gene | forward | 5030..5940

## Sequence

g ctcatcaagcttcgcatagagc aaaacagtgactagcacttttgtatgtaattgaaattccaaaattccaatttttagctgaaataacgtggaagctatagcatggcaagtttaatttttaaatacaatcattgcgtaaaactgtggtgaattatataaagacataaaaagatttctgaaTTAATCAATAATCAGACGCTTATTTGAAAGACTTTCGAGGGCTCTTGGTGATGGTGTGAACGCTCGGAATGCAAGAAAATCATCAGAAATATTAATCTTGGGACGTTCAGCGTCATGTGTAGCTCCAATGAAAACGACTTCTCCTCGATTCAATACTTGTGATGCATCTCCCAATGTAACTGTTGCAGTACCATAGAGAACCGTCAAGACAGAACATGATTCGGAGTAAGGAAGTTGGTAGATCGATCCAGCGGGTCCTTTG ACTTCCTGCACCCAGAACTCATCAATTCCA CGTGGAGTAAATAGTAGTGA TCCATCGTCCAGTTCATTTGGAATATATTTTGGAAGCAGGGTCTCATCATAATTCAACATTTCAACCAATGATTCTACATCAATATATTTTGGTGTTAATCCAGCTCGAATAGTATTGTCACTGTCTGCCATACACTCAACACAATCTCCTTTCAAATAAGCATGTGGCATGTTTGGTTCTAAAAATGTTGCCTCTCCAGGTTGTAATTTGAAGTAGTTCAAGAAAATTGGAGCAAATACACCAACATCTCCAGGGTACAATGTGAAAAGGTAGACAATTATTTCATCAAGAGCCGTTTTGTTTTctgaaatttagtaattaaaaagaagatattcattcaaaaatatcgcttacCATGTCCTTGAATTCGTCTGGCAAGTTTATCAACAACTATTTGAAGTTTTTCCTTTGGAGTTCGCCAAATTCTTGAGAAGATCTTTTTGA GAACCTGTGCAGAACTCTCTCC CCATCTAATGCAAGACTTCC AGCCATGCTAGAATTTCCTTTGGGTCCCCA TGCGTAGTTATTGACAGTGCACTTTAGCTTCAGAAGCATctgaaaacatgaattatggaagaaatgttttgagagaacagattatgttcgaaatatgagttagggagaaaataaaagatataatgcatcaaaatgacggtaaacgactgatctctctagttttctctgatcaacgcatgcgctaaaatcgacacgtgactaaatataatcagtcataagttgaccataagacaagagaaaaagacttccgaactggaagacacggaatacatatatatgaccgagaggctcggagacgcagattgaaaacgctgagatcagaactataaaacgagtgcgaccgtcgacttttttcatatcttgagtgggtatgcgtgtgagattgcgtcaacgtgactgcctaaactaattgtagagaaa ggcgtgctctactaaactcctc ctaatgtttttttgttgttttttcaattgccactgtttttttttctaatttcaataaaactgaatcctttttaaattgtttttttcag