# File: lmbr-1_Wildtype.ape

## Comments

>R05D3.2a.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 6951 bp
>Deletion size = 1876 bp
>lmbr-1 sgRNA #1, Chr III, GGCCCAATACGCCCTGGAGG; cut=8372289; strand=+1; GC=70; 3'GG=yes; folding=-3.2
>lmbr-1 sgRNA #2, Chr III, GACATGCTCTCTAATCATGG; cut=8374145; strand=+1; GC=45; 3'GG=yes; folding=-1.6

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
lmbr-1-1 SV F | primer_bind | forward | 1051..1072
lmbr-1 Upstream Homology Arm | misc_feature | forward | 1553..2002
Left Deletion Flank | misc_structure | forward | 1973..2002
lmbr-1 sgRNA #1 | protein_bind | forward | 2003..2022
lmbr-1-1 WT R | primer_bind | reverse | 2812..2833
lmbr-1 sgRNA #2 | protein_bind | forward | 3859..3878
Right Deletion Flank | misc_structure | forward | 3879..3908
lmbr-1 Downstream Homology Arm | misc_feature | forward | 3879..4328
lmbr-1-1 SV R | primer_bind | reverse | 4469..4490
met-2_Unspliced_UTR | gene | forward | 5031..6951

## Sequence

ctttttgtggcgaaagaaaccg taaattagtcaaaggaagacaaaaagaattcctccttgtttcaattcactatctcgctctttaccacctgtatagctaatcaatttcgaatttttcatatgaaaatgcatgtttttttcaaaactgtttttagcaattattttagaatttccaaaacctccaataatttcgattcaatgctttgataagttaaccgcttttataaatgctctttacacgttgagttcttgaagttttttcttttcacttgacgaatttacgaggtcatgttgtaacattgtccccgccgaaatgaaatagaaaacgcactcaaaaaacactctgacctcataaccaattttctattggccgctatctattttcaaattgtaaaaaaaagatttaccgaaattcgactccacaacaaagagcttctccaccgggacgaaatacgacaatttccggcaacctgttttcaatatgtcacatgtgtttgtgtct ttctaactaactttcaaacaccgatatcttttttctaagtttctaaccgaaccgatgagaacaatctgggagatttttgagacttcccagttctattcggttatccactcgtaggttccaacaattgaactttgtccttttcaattatatctgttaaatttcatccctatcaatgataacatagtgaccctttataaggtcttcgtgcttctactgtgaatgttttgataaggtgatacgatttatctcttccttcgcataccaaatttttttaaaaacgaactgtgcctctgtgacaaggaacatgtgtgcatgaatataataaaacgccttcactttcttcttttaatttctggtatacccaatttctcattgtgccttcaatttcttatttggcaagaattatgaaaaatttctcta ttgctttttacagatttaataacaccaaAT GGCCCAATACGCCCTGGAGG CCATCCTCGACTACCCTGTATC GACATGCTCTCTAATCATGG TGGCTACAAATACCTTGAAATTGTTATTCG GGTATCGCAGTTTACCTGTCTATGCTCAATACATTGAGgtaagcttcggagattttaaatataatttctaaaagtacataatttttcagGTACACACTCGTCATTCATTCGGACTTTTTGGGGCTTGCATCGAAACTCTTCTCATTATTTACGTGATGATCACATCTTTCGTTGGGCTGTACTCCCTTCCGGTTCTTCGTTCTCTTCGACCTGTTCGAAAAGATACTCCAATGCCAACGATAATTATTAATTCTTCAATAGTTCTTGTTGTTGCATCCGCTCTCCCAGTAGCTGTTAACACAGTTGGAATGACAACTTTTGATCTTCTCGGCTCCCACTCATCGCTCCAATGGCTTGGATCATTTCGAGTCGTTGTTGCCTATAATACTCTATTCGTCGTGTTGTCTGTCGCATTTCTCT TCAATCAATTGACTGCTTCAATGAGAAGGCAAATCTGGAAGTGgtaagctgtgcaatttaaagtttaaattcttattaatttttttgcagGATATGTCAACTACGATGTGGAATCAGACGGGAGAGTGATGCGGATGAAA CCATTGAGATCCTTAGAGGCGA