# File: lmbr-1_Deletion.ape

## Comments

>R05D3.2a.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 6951 bp
>gene: lmbr-1; chr: III; strain: VH7188; allele: hd7180; deletion size: 1876 bp; type: Heterozygous; QC: PPHP; outcrossed: 0
>Deletion size = 1876 bp
>lmbr-1 sgRNA #1, Chr III, GGCCCAATACGCCCTGGAGG; cut=8372289; strand=+1; GC=70; 3'GG=yes; folding=-3.2
>lmbr-1 sgRNA #2, Chr III, GACATGCTCTCTAATCATGG; cut=8374145; strand=+1; GC=45; 3'GG=yes; folding=-1.6

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
lmbr-1-1 SV F | primer_bind | forward | 1051..1072
lmbr-1 Upstream Homology Arm | misc_feature | forward | 1553..2002
Left Deletion Flank | misc_structure | forward | 1973..2002
Selection Cassette | variation | forward | 2003..7419
LoxP | misc_recomb | forward | 2024..2057
Pmyo-2 | regulatory:promoter | forward | 2100..3078
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 2605..2628
GFP(S65C) | CDS | forward | 3106..3276,3328..3481,3533..3696,3748..3975
unc-54 3'UTR | 3'UTR | forward | 4112..4846
Prps-27 | regulatory:promoter | forward | 5045..5838
neoR | CDS | forward | 5839..6633
NeoR SEC Insertion Primer 2 | primer_bind | forward | 6140..6161
unc-54 3'UTR | 3'UTR | forward | 6634..7332
LoxP | misc_recomb | forward | 7363..7396
lmbr-1 Downstream Homology Arm | misc_feature | forward | 7420..7869
Right Deletion Flank | misc_structure | forward | 7420..7449
lmbr-1-1 SV R | primer_bind | reverse | 8010..8031
met-2_Unspliced_UTR | gene | forward | 8572..10492

## Sequence

ctttttgtggcgaaagaaaccg taaattagtcaaaggaagacaaaaagaattcctccttgtttcaattcactatctcgctctttaccacctgtatagctaatcaatttcgaatttttcatatgaaaatgcatgtttttttcaaaactgtttttagcaattattttagaatttccaaaacctccaataatttcgattcaatgctttgataagttaaccgcttttataaatgctctttacacgttgagttcttgaagttttttcttttcacttgacgaatttacgaggtcatgttgtaacattgtccccgccgaaatgaaatagaaaacgcactcaaaaaacactctgacctcataaccaattttctattggccgctatctattttcaaattgtaaaaaaaagatttaccgaaattcgactccacaacaaagagcttctccaccgggacgaaatacgacaatttccggcaacctgttttcaatatgtcacatgtgtttgtgtct ttctaactaactttcaaacaccgatatcttttttctaagtttctaaccgaaccgatgagaacaatctgggagatttttgagacttcccagttctattcggttatccactcgtaggttccaacaattgaactttgtccttttcaattatatctgttaaatttcatccctatcaatgataacatagtgaccctttataaggtcttcgtgcttctactgtgaatgttttgataaggtgatacgatttatctcttccttcgcataccaaatttttttaaaaacgaactgtgcctctgtgacaaggaacatgtgtgcatgaatataataaaacgccttcactttcttcttttaatttctggtatacccaatttctcattgtgccttcaatttcttatttggcaagaattatgaaaaatttctcta ttgctttttacagatttaataacaccaaAT ccgcggcgcatcaggcggcct ataacttcgtatagcatacattatacgaagttat tacgtagagctcggtacctcgtacgtaatacgactcactagt acaagtttgtacaaaaaagcaggctta ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGG gtaagtttaaacatatatatactaactaaccctgattatttaaattttcag CCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC gtaagtttaaacagttcggtactaactaaccatacatatttaaattttcag GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTT gtaagtttaaacatgattttactaactaactaatctgatttaaattttcag AACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG cattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcag agatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaac ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG ATCTCCTGTCATCTCACCTTGC TCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA ctcgaggtcgacaggcctgcatgcgtcgac ataacttcgtatagcatacattatacgaagttat agttgcaggaccactgcggccgc TGGCTACAAATACCTTGAAATTGTTATTCG GGTATCGCAGTTTACCTGTCTATGCTCAATACATTGAGgtaagcttcggagattttaaatataatttctaaaagtacataatttttcagGTACACACTCGTCATTCATTCGGACTTTTTGGGGCTTGCATCGAAACTCTTCTCATTATTTACGTGATGATCACATCTTTCGTTGGGCTGTACTCCCTTCCGGTTCTTCGTTCTCTTCGACCTGTTCGAAAAGATACTCCAATGCCAACGATAATTATTAATTCTTCAATAGTTCTTGTTGTTGCATCCGCTCTCCCAGTAGCTGTTAACACAGTTGGAATGACAACTTTTGATCTTCTCGGCTCCCACTCATCGCTCCAATGGCTTGGATCATTTCGAGTCGTTGTTGCCTATAATACTCTATTCGTCGTGTTGTCTGTCGCATTTCTCT TCAATCAATTGACTGCTTCAATGAGAAGGCAAATCTGGAAGTGgtaagctgtgcaatttaaagtttaaattcttattaatttttttgcagGATATGTCAACTACGATGTGGAATCAGACGGGAGAGTGATGCGGATGAAA CCATTGAGATCCTTAGAGGCGA