File: lmbr-1_Wildtype.ape

Comments
>R05D3.2a.1 unspliced + UTR + 2000 upstream + 2000 downstream; +ive
 strand; Sequence: 6951 bp
>Deletion size = 1876 bp
>lmbr-1 sgRNA #1, Chr III, GGCCCAATACGCCCTGGAGG; cut=8372289;
 strand=+1; GC=70; 3'GG=yes; folding=-3.2
>lmbr-1 sgRNA #2, Chr III, GACATGCTCTCTAATCATGG; cut=8374145;
 strand=+1; GC=45; 3'GG=yes; folding=-1.6



Features
lmbr-1-1 SV F	primer_bind	forward	1051..1072
CTTTTTGTGGCGAAAGAAACCG

lmbr-1 Upstream Homology Arm	misc_feature	forward	1553..2002
TTCTAACTAACTTTCAAACACCGATATCTTTTTTCTAAGTTTCTAACCGAACCGATGAGAACAATCTGGGAGATTTTTGAGACTTCCCAGTTCTATTCGGTTATCCACTCGTAGGTTCCAACAATTGAACTTTGTCCTTTTCAATTATATCTGTTAAATTTCATCCCTATCAATGATAACATAGTGACCCTTTATAAGGTCTTCGTGCTTCTACTGTGAATGTTTTGATAAGGTGATACGATTTATCTCTTCCTTCGCATACCAAATTTTTTTAAAAACGAACTGTGCCTCTGTGACAAGGAACATGTGTGCATGAATATAATAAAACGCCTTCACTTTCTTCTTTTAATTTCTGGTATACCCAATTTCTCATTGTGCCTTCAATTTCTTATTTGGCAAGAATTATGAAAAATTTCTCTATTGCTTTTTACAGATTTAATAACACCAAAT

Left Deletion Flank	misc_structure	forward	1973..2002
TTGCTTTTTACAGATTTAATAACACCAAAT

lmbr-1 sgRNA #1	protein_bind	forward	2003..2022
GGCCCAATACGCCCTGGAGG

lmbr-1-1 WT R	primer_bind	reverse	2812..2833
CCATCCTCGACTACCCTGTATC

lmbr-1 sgRNA #2	protein_bind	forward	3859..3878
GACATGCTCTCTAATCATGG

Right Deletion Flank	misc_structure	forward	3879..3908
TGGCTACAAATACCTTGAAATTGTTATTCG

lmbr-1 Downstream Homology Arm	misc_feature	forward	3879..4328
TGGCTACAAATACCTTGAAATTGTTATTCGGGTATCGCAGTTTACCTGTCTATGCTCAATACATTGAGGTAAGCTTCGGAGATTTTAAATATAATTTCTAAAAGTACATAATTTTTCAGGTACACACTCGTCATTCATTCGGACTTTTTGGGGCTTGCATCGAAACTCTTCTCATTATTTACGTGATGATCACATCTTTCGTTGGGCTGTACTCCCTTCCGGTTCTTCGTTCTCTTCGACCTGTTCGAAAAGATACTCCAATGCCAACGATAATTATTAATTCTTCAATAGTTCTTGTTGTTGCATCCGCTCTCCCAGTAGCTGTTAACACAGTTGGAATGACAACTTTTGATCTTCTCGGCTCCCACTCATCGCTCCAATGGCTTGGATCATTTCGAGTCGTTGTTGCCTATAATACTCTATTCGTCGTGTTGTCTGTCGCATTTCTCT

lmbr-1-1 SV R	primer_bind	reverse	4469..4490
CCATTGAGATCCTTAGAGGCGA

met-2_Unspliced_UTR	gene	forward	5031..6951
 


formatted sequence

        1 aaatctttgt gtaacattga atacagtttt tttttaattt ttgtatttaa taataaatgc
       61 aaatcacttt caaattcaaa ctcgcgcccc cactctcctc tcggtctctc cattcgacgt
      121 gctctctagc tgcataggtc tcaccacgca gcctttgtga actcctctcg gagtacttaa
      181 atttttttta gtttattaat tttaacagtt atttctttcg ttttcttggg ggttccaatg
      241 ttataaagca caaaaactat tataatagat ttattaaatt tagaaaattg ctaaagcgtg
      301 atttttaaca gaaacactgt tttccttgcg tgcagaagtg atattggctt cctataggcc
      361 tttaagactt tagggtttaa gactgtctat acaggtgtaa aacataagtt ttagttattg
      421 ctatgcgaat aatttcgatt tttctaaagt tgtgaaatga accatcttat ggtttcgaaa
      481 aaaaaacaat gttgacattg ttttctgttc tcttgaattg acatcatttt taacgcaaaa
      541 ataattaaat accataaatt agagctcaac tgatcaataa tacaaccttt cagcacgtga
      601 gagtgcgtgt ttcctcctcc tccaaccagc catctcgatt cgaatcgaca cgctctttgt
      661 ctttctatgt ctcttatttc ctcatgctct ctcggttttc ttcttcgacg cgttcaaatt
      721 gtaagagtgg ttgcaccact gagcgtccgt gcatggttct gatccatttt tatgttttta
      781 ttttgttttt tttttttcga attttcacgt gaaaaaacca gtttctgttt ccttttcagt
      841 gtctcttatc taatcttgat tacccatttc acatttcgcc tcactctctt ttcacgcagc
      901 ctttacactt tgaacccttc tttttatgtc gttgtttttc tttgcaatca ctaaaatcac
      961 acaggagttg gaaattttta cttgcgaaat ttaagtcaac gtcgacctat tatctttatt
     1021 gacacatata tttttctcgc gtttcggatg ctttttgtgg cgaaagaaac cgtaaattag
     1081 tcaaaggaag acaaaaagaa ttcctccttg tttcaattca ctatctcgct ctttaccacc
     1141 tgtatagcta atcaatttcg aatttttcat atgaaaatgc atgttttttt caaaactgtt
     1201 tttagcaatt attttagaat ttccaaaacc tccaataatt tcgattcaat gctttgataa
     1261 gttaaccgct tttataaatg ctctttacac gttgagttct tgaagttttt tcttttcact
     1321 tgacgaattt acgaggtcat gttgtaacat tgtccccgcc gaaatgaaat agaaaacgca
     1381 ctcaaaaaac actctgacct cataaccaat tttctattgg ccgctatcta ttttcaaatt
     1441 gtaaaaaaaa gatttaccga aattcgactc cacaacaaag agcttctcca ccgggacgaa
     1501 atacgacaat ttccggcaac ctgttttcaa tatgtcacat gtgtttgtgt ctttctaact
     1561 aactttcaaa caccgatatc ttttttctaa gtttctaacc gaaccgatga gaacaatctg
     1621 ggagattttt gagacttccc agttctattc ggttatccac tcgtaggttc caacaattga
     1681 actttgtcct tttcaattat atctgttaaa tttcatccct atcaatgata acatagtgac
     1741 cctttataag gtcttcgtgc ttctactgtg aatgttttga taaggtgata cgatttatct
     1801 cttccttcgc ataccaaatt tttttaaaaa cgaactgtgc ctctgtgaca aggaacatgt
     1861 gtgcatgaat ataataaaac gccttcactt tcttctttta atttctggta tacccaattt
     1921 ctcattgtgc cttcaatttc ttatttggca agaattatga aaaatttctc tattgctttt
     1981 tacagattta ataacaccaa ATGGCCCAAT ACGCCCTGGA GGCGGGTGTC AGCTGGCTGG
     2041 CCACATCTGT ATCAGTGGTG GCCAGCGGAA CTTGGCAGTT TGCCAAATGG ACACACAAAT
     2101 ATGTGATGCA GCAAGCCGAA GAACTGGAAG CAGATGAACC AGAGGAATCG TATTTCCAAC
     2161 AGATGGTTGA TAAGGAAAAG GAGTTTCACA ATTATGTTCG ACAACAAATT gtaagcttaa
     2221 tttttatttg cgaaaatgtt caaatgtcta acttacagAT CTGCATGTGG CTATTCATGT
     2281 TACTATATCT ATTTGCATAT TGGCTTATAT CAAGATTAAA ACGAAAAACG GAAAGAGAAG
     2341 CACTGTATGC TGGAGAGGAA GACTATTTTG TCTACCGAGT ATCgtgagtt tatcattttt
     2401 tgcctattaa cccaataaaa taaatttcag TGTATGGATC AGCAGTACAG CAACAGCAAC
     2461 ATCAATAGGA TCCCTAACAC TGTTGCCGTT CTCCGTTATT GGAGTAGAAC TACTTCAGCT
     2521 TTATGATGGA AACTACTACT TACAGTGGTT GTCATATTCG TTGATTGGAG CTCTTTGGAA
     2581 TTACGTTTTC GTTCTTTCCA ACGTCTCGTT ATTTGTTCTA TTGCCATTTT CTTATTTTTT
     2641 CATCGAATCT CAAGGGTTTT CGACGTCGAA AATTGGAAAT gtaggtttct tagttttatt
     2701 cgtgttatat atttttctag tttcagGACA TGACTCAACG CATCTACGAA GCCATGGCGA
     2761 TTTCTTTTCT GTTCGCATTC GTTCTTCTGT GCCTCGCGGA AGTTGTTTTA ACCATCCTCG
     2821 ACTACCCTGT ATCATTTCTT TCGATCACTT CTGTCAATCT TCCATTGATC TACTCATGTG
     2881 TCAGTTTTAT CGGAGCGGTT CTTCTCCTGA TTTCGACTCC ATATGGGTTC GCGAAAATGT
     2941 TCTCTCTGGC CAGAGATTTT TTGGTTACGG AGGAAACCGC AGACATCGAG GAAGAGAATT
     3001 CAGAACAATC AGAAGATGTA ACAGAGCCGA AAAATTCAAG TTCTGATGAA ACGATACATC
     3061 AAGTTGATCG CAGCGATACT CCACATCTAG AAGACGTGGT CAATGATATA ACTGAAAACG
     3121 TTGATGCAGA TGGAGAGTTT AGAAAGGATA GTGATAGCGG AATCGAATCT GGATCAACTG
     3181 AAGAAATGCG GCTGAACACT GATGACGAAG AGATGGGAAT AAATGATTCG GATGATAAGT
     3241 CAGCGTTTGG TGATGATGGA GATTTAGGAA ACTCAACACC CACCAAAAAT AAAAAGAAGA
     3301 GGAGGAAACA TGATTATGCT GCGACAACAC CTATAGTTCG AAAATGGGAT AAAGATGTTC
     3361 CAAAAAAGCC AAAGAATCCA AATTTCGACT ATAGGAATCT GAAAGAGTAT GTCAAAGAGG
     3421 CTCGCCGGCA GAGGTCATCC CTTTCTGAAT CTGACGATTA TTGGTTTGGG TCACCTCCAA
     3481 GGAGCTCATT TTCGGCAAAT TATTATTCTT CAAGgtattt aatttttgta tgttttaaac
     3541 agttttattt aacaatgttg atttaagATT TTCTCGCTGG AAACACAATT CCGAAACTGG
     3601 CCTCAATCCA TCCTCATCAC TTCTTGTGGA TCCTTTTGCT TCTGGAGATT TCCATGAAGC
     3661 ATCTTCGTCT GAGGCATCAT CAACACCATT ATCACCTGCT CGTCGCACCA AATCCGAGGA
     3721 AGCCATCTGG AAGCCAGTTC TTCACACAGT AAAATCCTCG AAATTATACA AAAGAGCAAT
     3781 TGAGAAGCAG GGGCGACTTG TGAAACTTTT CATGAGTCTT CGATTCCCAG TAGCGGCCGC
     3841 TGCTTTGTTA GTTTTGACGA CATGCTCTCT AATCATGGTG GCTACAAATA CCTTGAAATT
     3901 GTTATTCGGG TATCGCAGTT TACCTGTCTA TGCTCAATAC ATTGAGgtaa gcttcggaga
     3961 ttttaaatat aatttctaaa agtacataat ttttcagGTA CACACTCGTC ATTCATTCGG
     4021 ACTTTTTGGG GCTTGCATCG AAACTCTTCT CATTATTTAC GTGATGATCA CATCTTTCGT
     4081 TGGGCTGTAC TCCCTTCCGG TTCTTCGTTC TCTTCGACCT GTTCGAAAAG ATACTCCAAT
     4141 GCCAACGATA ATTATTAATT CTTCAATAGT TCTTGTTGTT GCATCCGCTC TCCCAGTAGC
     4201 TGTTAACACA GTTGGAATGA CAACTTTTGA TCTTCTCGGC TCCCACTCAT CGCTCCAATG
     4261 GCTTGGATCA TTTCGAGTCG TTGTTGCCTA TAATACTCTA TTCGTCGTGT TGTCTGTCGC
     4321 ATTTCTCTTC AATCAATTGA CTGCTTCAAT GAGAAGGCAA ATCTGGAAGT Ggtaagctgt
     4381 gcaatttaaa gtttaaattc ttattaattt ttttgcagGA TATGTCAACT ACGATGTGGA
     4441 ATCAGACGGG AGAGTGATGC GGATGAAACC ATTGAGATCC TTAGAGGCGA TAAGAAAAGC
     4501 AATTGAattt ctttcctttt tcaacacttc ttacccatgt tcatcatttt aatcttttca
     4561 ttacaaaaac aaggtcctat tttttttctc gggtactact cgccttttct aataattcag
     4621 aatcatcaat ttttgccaac ctctagcttt acatgtctgt ttttcatcat tttctctcaa
     4681 gcattctcct aatatattat gttccctagt atttcccctc agtcagcaat tttctcgtcg
     4741 tcgaaaccgt ttagctttac tttcaatcaa aacgtggaac atttttcaaa ctatttgaag
     4801 ccaaaaaaaa ccagggcttt tgtatatgta ccatattttc cctctgattt tctttatcgc
     4861 cttctctttt catgtagaat aactgaaata caaaccattt taattttttc ttttaattat
     4921 caatactgtc cgtataggta aaaattattt cttcaggttt gaaaaaatcc gaaatatgta
     4981 tctgcaactc ttcagggcat tgcctcaatt aatttttatc taatattcag atggaccaac
     5041 aagaaccatc gaataacgta gatacgagca gtattctttc ggatgatggg atggaaacac
     5101 aggaacaaag ttcattcgtc actgctgtga gtgaaattat ttaaaatttc gcttcggaga
     5161 ttcattgtca tataattcaa tttatcgatt ttcagacaat tgacctaaca gtggacgact
     5221 acgatgaaac agaaatacag gagattctgg ataatggaaa agcagaagaa ggaacagatg
     5281 aagattctga tttagttgaa gggattctta acgctaattc agatgtccaa gcgctccttg
     5341 atgcgccatc tgagcaagta gctcaagctc ttaattcgtt cttcggaaat gagagtgaac
     5401 aagaagctgt tgcagcacaa agacgggttg atgcggagaa gactgccaaa gatgaagctg
     5461 aactcaagca acaggaagag gcggttagat tgcaataaag gaaacaataa taaaattatt
     5521 ttattttcag gaagatctta ttatagaaga ttcgatagtc aaaactgatg aagaaaaaca
     5581 agcagttcga agactgaaaa tcaacgaatt tttatcgtgg ttcacaaggc tccttccaga
     5641 acaatttaaa aatttcgaat tcacaaatcc gaactatctg acagaatcta tcagcgattc
     5701 accggttgta aatgtcgata aatgcaagga aattgtcaaa tcgttcaagg aaagtgaatc
     5761 acttgaggga ctttcacaga aatacgaatt aattgatgaa gacgtgctag tcgctgctat
     5821 ttgtattggc gttctcgata ccaacaacga agaagatgtc gactttaatg ttctatgtga
     5881 tgatcgtatc gacgattgga gtatagaaaa atgtgtcact tttcttgatt atccaaatac
     5941 tggattgaat tcgaaaaatg gaccgttgag attcatgcag tttactgtca catcacctgc
     6001 atcagcaatt ctcatgctca ctctgattcg attacgcgaa gaagggcatc cgtgtcgatt
     6061 agattttgat tcaaatccga ctgatgattt actcttgaat ttcgatcaag tggaattttc
     6121 taataatatc attgatacgg cagtcaaata ctgggatgat cagaaggaaa acggtgcgca
     6181 ggataaaatt ggcaggcgag tattaatcaa actcacaact gttttgaaag tattttcata
     6241 attatcactt aaataccttt tagagagctc aacgacttct tccacgaaat cgagtcaaca
     6301 tcagcagaat tcaaacaaca ttttgagaac gccgttggca gccgtaatga aataattcaa
     6361 cttgtcaacg agaaaattcc cgattttgat ggcactgagg ctgctgtgaa tgagagtttt
     6421 acatccgatc aacgaaccga aattatcaac tctcgtgcaa taatggagac attaaaagcc
     6481 gagatgaagc tcgccatcgc cgaagctcag aaagtttacg acaccaagac tgacttcgaa
     6541 aaattcttcg ttttgacagt tggagatttc tgtctggctc gcgccaatcc ttctgacgat
     6601 gcagaattaa catacgccat agttcaggat cgtgtggatg caatgaccta taaggttaaa
     6661 tttatcgaca caagtcagat cagagagtgt aacatcagag atttagccat gactacgcag
     6721 ggaatgtatg acccgagttt gaatacattt ggtgatgttg gtgagtttta agttaaaatt
     6781 gatatttaat attacatctg ttatgtagaa taagggtttc ggtttttcga ttttattaga
     6841 aaatcgaaaa ttttagtttt tgtgttaaat ttaaaaaaat caaaatttga ttcactatca
     6901 agtccgtttt tctcttctca aaattgacaa aattttgata atctagaatt t
//

plain sequence
 
