# File: algn-5_Deletion.ape

## Comments

>H43I07.3 unspliced + UTR + 2000 upstream + 2000 downstream; +ive strand; Sequence: 7823 bp
>gene: algn-5; chr: V; strain: VH7186; allele: hd7175; deletion size: 1325 bp; type: Heterozygous; QC: PFHP; outcrossed: 0
>Deletion size = 1325 bp
>algn-5 sgRNA #1, Chr V, GACTTTCCTACGCAACACCA; cut=4195777; strand=+1; GC=50; 3'GG=no; folding=0.0
>algn-5 sgRNA #2, Chr V, ATTCTCTTCGCAGATGCCGA; cut=4197082; strand=+1; GC=50; 3'GG=no; folding=-1.5

## Features

Name | Type | Orientation | Position
--- | --- | --- | ---
algn-5-1 SV F | primer_bind | forward | 1354..1374
algn-5 Upstream Homology arm | misc_feature | forward | 1834..2183
Left Deletion Flank | misc_structure | forward | 2154..2183
Selection Cassette | variation | forward | 2184..7600
LoxP | misc_recomb | forward | 2205..2238
Pmyo-2 | regulatory:promoter | forward | 2281..3259
pMYO-2 SEC Insertion Primer 2 | primer_bind | reverse | 2786..2809
GFP(S65C) | CDS | forward | 3287..3457,3509..3662,3714..3877,3929..4156
unc-54 3'UTR | 3'UTR | forward | 4293..5027
Prps-27 | regulatory:promoter | forward | 5226..6019
neoR | CDS | forward | 6020..6814
NeoR SEC Insertion Primer 2 | primer_bind | forward | 6321..6342
unc-54 3'UTR | 3'UTR | forward | 6815..7513
LoxP | misc_recomb | forward | 7544..7577
Right Deletion Flank | misc_structure | forward | 7601..7630
algn-5 Downstream Homology Arm | misc_feature | forward | 7601..7950
algn-5-1 SV R | primer_bind | reverse | 8837..8858

## Sequence

agcatgcttagtacctgctgc cccccggcaactcgcttgtttggccttcaatcgattagcgccctgccggtgagtgctgacgcatctctcgtgtcacttgcacccgcttttctcccaccgatttttgcacattgaaagtgccacgggggctcaatgcaaatagaaaattgaacaagattgagccttgttttaattcttttgtgcatctttttttctgaattagttttttcttcatttttattattttaggctgtgatatttaaagtttctttagaaaatcgtcttgaaattgaagggaaaacgaatataaatcgaaaaaatcgaaaaaaaaggaatttaatttaaaaatcaatttaaactgatttttgttttcccatgcgcctttaatttttacagtatccttttttccgaaaatgaactatttttgcctattcaacattcaatttttgctaaaatactttattttgcttatttcaatgt gttttaaaatattattaatacaaaaattcgttttttttgctccttaaattgaaacaattgagcaactgatggttttcgttgcgagacccgggcttaaatttctataacgcgcctttaaaagtcctacggtttgcttttttttcgttaaatttttattttcgaaaaccaaagcttattccgatttaatctcgattttaattccagaaATGTGGGATTTAATTTCGCCGGAAATTTTCTCAATACAAAATCTTTGCCTGCTGGGATTTCTCGTCGCCTCAATCGCCGTGAGCAGCCTCTTCACgtaagtttttcgatttttc tccaaaaaatcaatatcttcaccattttca ccgcggcgcatcaggcggcct ataacttcgtatagcatacattatacgaagttat tacgtagagctcggtacctcgtacgtaatacgactcactagt acaagtttgtacaaaaaagcaggctta ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGG gtaagtttaaacatatatatactaactaaccctgattatttaaattttcag CCAACACTTGTCACTACTTTCTGTTATGGTGTTCAATGCTTCTCGAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC gtaagtttaaacagttcggtactaactaaccatacatatttaaattttcag GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTT gtaagtttaaacatgattttactaactaactaatctgatttaaattttcag AACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG cattcgtagaattccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcag agatcttcgaatgcatcgcgcgcaccgtacgtctcgaggaattcctgcaggatatctggatccacgaagcttcccatggtgacgtcaccggttctagatacctaggtgagctctggtaccctctagtcaaggccttaagtgagtcgtattacggactggccgtcgttttacaggccggatgggccatggcaccttaac ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG ATCTCCTGTCATCTCACCTTGC TCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCACCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA ctcgaggtcgacaggcctgcatgcgtcgac ataacttcgtatagcatacattatacgaagttat agttgcaggaccactgcggccgc TGGAGCTACAAAATTCGCCGATTTTGAAAA TCTCGAGAAAGAAATGCTCCGAACTGCCGGCGGAGAGCCACTTGATGAATCATTTCCAGCAGTTATTGTTGCTTCTAGgtttttaattgatttttatagaaatttttaataattttcccattttcagAGCGCATTTGGAAGCCGAATCTATGGCTGTTAGATCAGTTCCACGCACAATTTTAATGCTCGGTTTTCACTTACTCGTCTATACTTTTGCTGCAAGAACAATTAAAGATACACAGTGTGGATTCAAACTTTTTACTAGATCGATTGCTGCGAGGgtgagattttagcttatttttagctaaaaatctccaatt CGATGGACTGAAATCGAAGGGT