Caenorhabditis Genetics Center (CGC)

Rearrangement Information

NamemnC1 View on WormBase
DescriptionGrowth characteristics: Homozygotes viable with extremely small broods (average: 7 progeny). Brood size in heterozygotes ~200, with 100% egg hatching. Recommended use: General balancing, strain construction, strain maintenance, mutant screens. Handling: Easy to manipulate. Heterozygous male stocks mate well. Very rare recombination gives rise to Dpy non-Unc and Unc non-Dpy progeny. The recombinant chromosomes carried by these progeny are homozygous lethal. Balancer Summary: Dominant crossover suppressor, uncharacterized with regard to structure, very stable. Very effective balancer for right portion of LG II from around dpy-10 to around unc-52.
Genetic positiongenetic position unknown or not listed
Genomic positiongenomic coordinates unknown or not listed

Strains carrying this rearrangement

Strain Genotype Species Description
SP758 unc-4(e120) mnDf92/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, paralysed DpyUnc and no viable Unc-4's. Maintain by picking WT.
SP759 unc-4(e120) mnDf93/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, paralysed DpyUnc and no viable Unc-4's. Maintain by picking WT.
SP760 unc-4(e120) mnDf94/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, paralysed DpyUnc and no viable Unc-4's. Maintain by picking WT.
SP781 unc-4(e120) mnDf97/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, dead eggs and DpyUnc. Maintain by picking WT. [2/97: This strain also throws Dpys. RK Herman put a new mnC1 dpy-10 unc-52 chromosome into the strains, and it still continues to throw Dpys. It seems that the mnC1 dpy-10 unc-52 chromosome is correct. It's possible that the mnDf97 chromosome is broken.]
SP782 unc-4(e120) mnDf98/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, paralysed DpyUnc and no viable Unc-4's. Maintain by picking WT.
SP783 unc-4(e120) mnDf99/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, DpyUnc and no viable Unc-4's. Maintain by picking WT.
SP784 unc-4(e120) mnDf100/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, paralysed DpyUnc and no viable Unc-4's. Maintain by picking WT.
SP787 unc-4(e120) mnDf95/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans
SP788 unc-4(e120) mnDf96/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are Dpy and segregate Dpy, dead eggs and paralysed DpyUnc. Maintain by picking Dpy.
SP789 unc-4(e120) mnDf101/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, paralysed DpyUnc and Unc-4 lethals that arrest at L2. Maintain by picking WT.
SP790 unc-4(e120) mnDf103/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, paralysed DpyUnc and no viable Unc-4's. Maintain by picking WT.
SP802 unc-4(e120) mnDf104/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, paralysed DpyUnc and no viable Unc-4's. Maintain by picking WT.
SP803 unc-4(e120) mnDf105/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, DpyUnc and no viable Unc-4's. Maintain by picking WT.
SP804 unc-4(e120) mnDf106/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, paralysed DpyUnc and no viable Unc-4's. Maintain by picking WT.
SP806 unc-4(e120) mnDf108/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, paralysed DpyUnc and no viable Unc-4's. Maintain by picking WT.
SP807 unc-4(e120) mnDf109/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Hets are WT and segregate WT, paralysed DpyUnc and no viable Unc-4's. Maintain by picking WT. [12/93 **mnC1 appears to have broken down**]
SS186 mes-2(bn11) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Uncs which give sterile progeny (maternal effect sterile: the progeny from mutant mothers are sterile). The mutation is a strict mel, fully penetrant, and fully expressed. Sterility is due to a failure in germ-cell proliferation.
ST29 ven-2(nc29)/mnC1 [dpy-10(e128) unc-52(e444)] II; ncIs3 III. C. elegans ncIs3 [pH20::GFP + pBlueScript]. Expresses GFP in nearly all neurons. Heterozygotes are WT and segregate WT, DpyUncs, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development.
SV122 lin-5(n3070)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, Stu and DpyUncs. n3070 is a strong loss-of-function or null allele. Molecular lesion: P to S at position 24 as well as an amber mutation terminating translation after amino acid 52. DNA replication continues in the absence of mitosis. Mutants enter mitotis at the normal time and form bipolar spindles, but fail chromosome alignment at the metaphase plate, sister chromatid separation and cytokinesis.
SV123 lin-5(n3066)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, Stu and DpyUncs. n3066 is a strong loss-of-function or null allele. Molecular lesion: ochre mutation terminating translation at amino acid 538. DNA replication continues in the absence of mitosis. Mutants enter mitotis at the normal time and form bipolar spindles, but fail chromosome alignment at the metaphase plate, sister chromatid separation and cytokinesis.
SV13 lin-5(e1348)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, Stu and DpyUncs. Molecular lesion: amber mutation terminating translation at amino acid 159. DNA replication continues in the absence of mitosis. Mutants enter mitotis at the normal time and form bipolar spindles, but fail chromosome alignment at the metaphase plate, sister chromatid separation and cytokinesis.
SV314 rol-1(e91) cyd-1(he112)/mnC1 [dpy-10(e28) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUncs, and rol-1 cyd-1 homozygotes which are thin, sterile, uncoordinated animals. rol-1 is largely suppressed by cyd-1. No postembryoinc cell divisions take place in cyd-1.
SV329 rol-1(e91) cyd-1(he116)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUncs, and rol-1 cyd-1 homozygotes which are thin, sterile, uncoordinated animals. rol-1 is largely suppressed by cyd-1. No postembryoinc cell divisions take place in cyd-1.
SV46 lin-5(e1457)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, Stu and DpyUncs. e1457 is a strong loss-of-function or null allele. Molecular lesion: G to E at position 40. DNA replication continues in the absence of mitosis. Mutants enter mitotis at the normal time and form bipolar spindles, but fail chromosome alignment at the metaphase plate, sister chromatid separation and cytokinesis.
VC2320 max-2(ok2553)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Y38F1A.10. Apparent homozygous lethal deletion chromosome balanced by recombination suppressor marked with dpy-10 and unc-52. Heterozygotes are WT and segregate WT, paralyzed Dpy mnC1 homozygotes and ok2553 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TGACAGAAATCGACAGCAGG. External right primer: ACGGGAACCCCCATATACTC. Internal left primer: AAGCGGTAAATGACGGAATG. Internal right primer: TGTGTCTGTGTGTCTTCGCA. Internal WT amplicon: 3342 bp. Deletion size: approximately 2000 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC274 arrd-3(ok536)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans M176.1. Heterozygotes are WT and segregate WT, paralyzed Dpy mnC1 homozygotes and ok536 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC341 hel-1(gk191)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans C26D10.2. Heterozygotes are WT and segregate WT, paralyzed Dpy mnC1 homozygotes and gk191 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VT132 sqt-1(sc13) lin-29(n333)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are slightly shorter than WT and segregate more animals which are slightly shorter than WT, Rollers which are Egl and have a protruding vulva, and DpyUncs.
VT516 lin-29(n546)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are slightly shorter than WT and segregate DpyUnc and Egl.
WM170 unc-4(e120) pir-1(tm1496)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUncs, and Unc-4 animals which arrest at the L4 stage. Rarely, a recombination will occur and unc-4 and pir-1 will become unlinked. Propagate the strain by picking single WT animals and checking for correct segregation of progeny. 6/2007: Daniel Chavez notes that tm1496 may also delete part of sec-5, which could be responsible for the developmental arrest of tm1496.