Caenorhabditis Genetics Center (CGC)

Rearrangement Information

NameeT1 View on WormBase
Description[Edgley M] Reciprocal translocation. Suppresses recombination on left arm of LG V and right arm of LG III. Breaks in unc-36. Homozygous viable with Unc-36 phenotype. Summary: Reciprocal translocation, well characterized, extremely stable. Very effective balancer for left portion of LG V from left end through unc-23, and right portion of LG III from right end to unc-36. eT1(III) is LG V (left) translocated to LG III (left), disjoins from normal LG III. eT1(V) is LG III (right) translocated to LG V (right), disjoins from normal LG V. Zhao et al. reports the sequence of the physical breakpoint of eT1 on chromosome V (the breakpoint of eT1 chromosome III is in the unc-36 gene). Growth characteristics: Original isolate homozygous viable with Unc-36 phenotype caused by the translocation breakpoint. Brood size in heterozygotes ~ 100, in homozygotes ~160. eT1 homozygotes can overgrow a population, especially in balanced strains carrying certain lethal mutations or deficiencies. Handling: Easy to manipulate. Heterozygous male stock mate well. 20-bp sequences of either side of the eT1 breakpoints are as follows, eT1(V): aataatttttgaaacaaaaa-GTTTATCATCTCAAGACGTG; eT1(III): GAGCCAATCACTTACTGGTTAGTAATGATTCCAAATATGAGCCAATCACTTACTGGTTAGTAATGATTCCAAA-cttgattccaaatatgacat Phenotype: Unc Recommended use: General balancing, strain construction, strain maintenance, and mutant screens. cross-over sup left of V, right of III;'allele' of unc-36(III); e873
Genetic positiongenetic position unknown or not listed
Genomic positiongenomic coordinates unknown or not listed

Strains carrying this rearrangement

Strain Genotype Species Description
BC3702 dpy-18(e364)/eT1 III; unc-46(e177) let-402(s1526)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT.
BC3712 dpy-18(e364)/eT1 III; unc-46(e177) let-474(s1577)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT.
BC3713 dpy-18(e364)/eT1 III; unc-46(e177) let-473(s1602)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. [This strain is acting strangely: it is throwing bent heads. 12/95]
BC3716 dpy-18(e364)/eT1 III; unc-46(e177) let-475(s1606)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adult) and dead eggs. Maintain by picking WT.
BC3718 dpy-18(e364)/eT1 III; unc-46(e177) let-407(s1631)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval (hatches). Maintain by picking WT.
BC3727 dpy-18(e364)/eT1 III; sDf56 unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC3728 dpy-18(e364)/eT1 III; unc-46(e177) let-480(s1607)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adult) and dead eggs. Maintain by picking WT.
BC3729 dpy-18(e364)/eT1 III; let-478(s1620) let-?(s2169) unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT.
BC3731 dpy-18(e364)/eT1 III; unc-46(e177) let-479(s1576)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adult-> Lays unfertilized eggs) and dead eggs. Maintain by picking WT.
BC3732 dpy-18(e364)/eT1 III; unc-46(e177) let-418(s1617)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adult; Vulva protrudes; partial rescue at 15C) and dead eggs. Maintain by picking WT.
BC3733 dpy-18(e364)/eT1 III; let-481(s1636) unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval (hatches). Maintain by picking WT.
BC3737 sDp8 (III;I); eT1 (III;V). C. elegans WT strain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC3953 dpy-18(e364)/eT1 III; sDf70 unc-46(e177)/eT1 [let-500(s2165)] V. C. elegans Heterozygotes are WT and segregate WT, no Unc36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC3954 dpy-18(e364)/eT1 III; unc-46(e177) sDf71/eT1 [let-500(s2165)] V. C. elegans Heterozygotes are WT and segregate WT and dead eggs. Embryonic lethal. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC3955 dpy-18(e364)/eT1 III; sDf72 unc-46(e177)/eT1 [let-500(s2165)] V. C. elegans Heterozygotes are WT and segregate WT and dead eggs. Embryonic lethal. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC3956 dpy-18(e364)/eT1 III; sDf73 unc-46(e177)/eT1 [let-500(s2165)] V. C. elegans Heterozygotes are WT and segregate WT, dead eggs, no Unc-36, and DpyUncLet. Lethal early larva. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC3957 dpy-18(e364)/eT1 III; sDf74 unc-46(e177)/eT1 [let-500(s2165)] V. C. elegans WT strain which segregates WT and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC3958 dpy-18(e364)/eT1 III; sDf75 unc-46(e177)/eT1 [let-500(s2165)] V. C. elegans Heterozygotes are WT and segregate WT and dead eggs. Embryonic lethal. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC4425 dpy-18(e364)/eT1 III; sDf48 unc-46(e177)/eT1 [let-500(s2165)] V. C. elegans Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC986 sDp3 (III;f); eT1 (III;V). C. elegans Throws Unc-36 and WT. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BW1739 +/eT1 III; unc-62(t2012) dpy-11(e224)/eT1 V. C. elegans Heterozygotes are wild-type, and segregate WT heterozygotes, Dpy Mel (unc-62 dpy-11 homozygotes), and dead eggs (eT1 homozygotes). unc-62 dpy-11 homozygotes give only dead embryos (96%) or dead larva (4%).
BW1747 dpy-18(e364)/eT1 III; unc-46(e177) let-427(s1057)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, Sterile DpyUncs and dead eggs. Maintain by picking WT.
CB4281 +/eT1 III; eDf43 dpy-11(e224)/eT1 V. C. elegans eDf43 pka lin-49(e2173). Heterozygotes are WT and segregate WT, Unc-36, and LinDpys. The lin-40 dpy-11 homozygotes are Dpy, Sterile and abnormal. Maintain by picking WT.
CB6121 eT5 (X; III); fog-2(q71) V females and eT5 (X; III) / eT1(III; V); fog-2 / eT1 (fog-2 V; III) males C. elegans Male/female strain, propagate by crossing. Females are eT5 (III;X); fog-2(q71) V. Males are eT5 (III;X) / eT1(III;V); fog-2 / eT1 (fog-2 III;V). Strain with multichromosomal sex determination. Reference: Strain 21 in Hodgkin (2002) PMID: 12399387.
CB873 eT1 (III;V). C. elegans M-MATING+POOR <1%WT. Recessive. Poor movement forward and backward. Unc-36 phenotype. Dead eggs.
CGC19 eT1 III; eT1 [umnIs8] V. C. elegans umnIs8 [myo-2p::GFP + NeoR, III:9421936 (intergenic)] V. Derived by insertion of myo-2p::GFP transgene into eT1 balancer in parental strain CB873 using CRISPR/Cas9.
CGC20 dpy-18(e364)/eT1 III; unc-46(e177)/eT1[umnIs9] V. C. elegans umnIs9 [myo-2p::GFP + NeoR, III:9421936 (intergenic)] V. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, Unc-36 GFP+ (eT1), dead eggs, and DpyUncs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9.
CGC23 dpy-18(e364)/eT1 [umnIs12] III; unc-46(e177)/eT1 V. C. elegans umnIs12 [myo-2p::GFP + NeoR, V: 1005689 (intergenic)] III. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, Unc-36 GFP+ (eT1), dead eggs, and DpyUncs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9.
CGC34 eT1 [umnIs12] III; eT1 V. C. elegans umnIs12 [myo-2p::GFP + NeoR, V: 1005689 (intergenic)] III. Derived by insertion of myo-2p::GFP transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9.
CGC55 eT1 [umnIs45] III; eT1 V. C. elegans umnIs45 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] III. Derived by insertion of myo-2p::mKate2 transgene into eT1 balancer in parental strain CB873 using CRISPR/Cas9.
CGC60 dpy-18(e364)/eT1 III; unc-46(e177)/eT1[umnIs46] V. C. elegans umnIs46 [myo-2p::mKate2 + NeoR, III:9421936 (intergenic)] V. Heterozygotes are wild-type mKate+, and segregate wild-type mKate2+, Unc-36 mKate+ (eT1), dead eggs, and DpyUncs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9.
CGC69 dpy-18(e364)/eT1 [umnIs55] III; unc-46(e177)/eT1 V. C. elegans umnIs55 [myo-2p::mKate2 + NeoR, V:1005689 (intergenic)] III. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, Unc-36 mKate+(eT1), dead eggs, and DpyUncs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9.
CGC70 eT1 III; eT1 [umnIs56] V. C.elegans umnIs56 [myo-2p::mKate2 + NeoR, III: 9421936 (intergenic)] V. Derived by insertion of myo-2p::mKate2 transgene into eT1 balancer in parental strain CB873 using CRISPR/Cas9.
DA1060 +/eT1 III; adDf1059/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs.
DG769 unc-32(e189) emb-30(tn476) III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36.
DG783 unc-32(e189) emb-30(tn478) III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36.
DG784 unc-32(e189) emb-30(tn477) III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Unc-32 maternal effect lethals (Mel) and Unc-36.
DG786 unc-32(e189) emb-30(tn480) III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36.
DG800 unc-32(e189) tnDf2 III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency).
DG832 emb-30(tn475)/eT1 III; unc-46(e177) mdf-1(gk2)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-46 Steriles, and Unc-36 (eT1 homozygotes). Maintain by picking WT.
EL186 dpy-19(e1259) ego-4(om30)/eT1 III; +/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Dpy (ts) Mel, Unc-36 and dead eggs. dpy-19 ego-4 progeny die as embryos.
EL187 dpy-19(e1259) ego-5(om31)/eT1 III; +/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Dpy (ts) Mel, Unc-36 and dead eggs.
FX291 +/eT1 III; spn-4(tm291)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. 1/3 of WT animals (spn-4 homozygotes) are Mels: segregate only dead embryos with have no morphogenesis. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
GC613 eef-1A.1(ar229) III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Steriles (homozygous ar229), Unc-36 (homozygous eT1), and dead eggs. ar229 have severely reduced germline proliferation.
JK1129 unc-32(e189) qDf2/eT1 III; +/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK1389 mog-1(q223) unc-69(e587)/eT1 III; +/eT1 V. C. elegans Heterozygotes are wild-type and segregate wild-type, Unc-36 (eT1 homozygotes), homozygous Mog (Unc, weak coiler). Maintain by picking wild-type. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK1505 unc-32(e189) glp-1(e2072) III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Sterile Unc coilers, Unc-36s (eT1 homozygotes) and dead eggs. Pick WT to maintain. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK2533 qC1 [dpy-19(e1259) glp-1(q339) qIs26] III/eT1 (III;V). C. elegans qIs26 [lag-2::GFP + rol-6(su1006)]. Throws heterozygous Rollers and Unc eT1 homozygotes. qIs26 was integrated into qC1 and in the process made qC1 homozygous lethal. The distal tip cells are GFP+. It was an integration of qEx233. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK590 glp-1(q35)/eT1 III; him-5(e1490)/eT1 V. C. elegans Heterozygotes are wild-type and segregate wild-type heterozygotes, glp-1(q35) homozygotes (Muv steriles), eT1 homozygotes (Unc-36), and males. glp-1(q35) has a semi-dominant multi-vulva phenotype as well as the loss-of-function Glp phenotype (sterility and embryonic lethality). The q35 mutation is a nonsense mutation that eliminates 122 C-terminal amino acids including a PEST sequence. The C terminus was thought to contain a negative regulatory domain that inactivates glp-1 in the VPCs; the inappropriate glp-1(q35) activity can substitute for lin-12 vulval fate determination. References: Austin J & Kimble J. Cell. 1987 Nov 20;51(4):589-99. Mango S, et al. Nature. 1991 Aug 29;352(6338):811-5.
JS71 dpy-11(e224) air-1(vw5) V/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Dpy Stu, Unc-36 (eT1 homozygotes) and dead eggs. Also weak Him.