Caenorhabditis Genetics Center (CGC)

Rearrangement Information

NameeT1 View on WormBase
Description[Edgley M] Reciprocal translocation. Suppresses recombination on left arm of LG V and right arm of LG III. Breaks in unc-36. Homozygous viable with Unc-36 phenotype. Summary: Reciprocal translocation, well characterized, extremely stable. Very effective balancer for left portion of LG V from left end through unc-23, and right portion of LG III from right end to unc-36. eT1(III) is LG V (left) translocated to LG III (left), disjoins from normal LG III. eT1(V) is LG III (right) translocated to LG V (right), disjoins from normal LG V. Zhao et al. reports the sequence of the physical breakpoint of eT1 on chromosome V (the breakpoint of eT1 chromosome III is in the unc-36 gene). Growth characteristics: Original isolate homozygous viable with Unc-36 phenotype caused by the translocation breakpoint. Brood size in heterozygotes ~ 100, in homozygotes ~160. eT1 homozygotes can overgrow a population, especially in balanced strains carrying certain lethal mutations or deficiencies. Handling: Easy to manipulate. Heterozygous male stock mate well. 20-bp sequences of either side of the eT1 breakpoints are as follows, eT1(V): aataatttttgaaacaaaaa-GTTTATCATCTCAAGACGTG; eT1(III): GAGCCAATCACTTACTGGTTAGTAATGATTCCAAATATGAGCCAATCACTTACTGGTTAGTAATGATTCCAAA-cttgattccaaatatgacat Phenotype: Unc Recommended use: General balancing, strain construction, strain maintenance, and mutant screens. cross-over sup left of V, right of III;'allele' of unc-36(III); e873
Genetic positiongenetic position unknown or not listed
Genomic positiongenomic coordinates unknown or not listed

Strains carrying this rearrangement

Strain Genotype Species Description
BC1230 dpy-18(e364)/eT1 III; sDf27 unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1282 dpy-18(e364)/eT1 III; unc-62(s472) unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. Unc-62 is a recessive lethal.
BC1283 dpy-18(e364)/eT1 III; unc-46(e177) sDf20/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1287 dpy-18(e364)/eT1 III; sDf26/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1289 dpy-18(e364)/eT1 III; sDf28 unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1381 dpy-18(e364)/eT1 III; unc-46(e177) sDf29/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1424 dpy-18(e364)/eT1 III; let-331(s427) unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Homozygous s427 is a cold sensitive sterile adult.(??) Maintain by picking WT.
BC1433 dpy-18(e364)/eT1 III; let-345(s578) unc-46(e177)/eT1 V. C. elegans WT strain which segregates WT, Unc-36, dead eggs, and DpyUncLets. Lethal mid-larval. Maintain by picking WT.
BC1434 dpy-18(e364)/eT1 III; let-330(s573) unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, DpyUncLet and dead eggs. Lethal mid larval. Maintain by picking WT.
BC1435 dpy-18(e364)/eT1 III; unc-46(e177) let-329(s575)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Some DpyUnc escapers are Sterile and have a protruding vulva.
BC1439 dpy-18(e364)/eT1 III; let-344(s376) unc-46(e177)/eT1 V. C. elegans WT strain which segregates WT, Unc-36, and dead eggs. (Egg lethal.) Maintain by picking WT.
BC1466 dpy-18(e364)/eT1 III; let-326(s238) unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, dead eggs and DpyUncLets. Lethal mid-larval. Maintain by picking WT.
BC1480 dpy-18(e364)/eT1 III; unc-46(e177) let-332(s234)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, and dead eggs. Egg lethal. Maintain by picking WT.
BC1481 dpy-18(e364)/eT1 III; let-350(s250) unc-46(e177)/eT1 V. C. elegans WT strain which segregates WT, Unc-36, dead eggs and DpyUncLet. Lethal late larval. Maintain by picking WT.
BC1483 dpy-18(e364)/eT1 III; unc-46(e177) let-419(s219)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT.
BC1519 dpy-18(e364)/eT1 III; sDf31/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. Original isolation: BO strain BC1261 unc-22(s727) hermaphrodite heat shocked for 1 hour at 34C. Crossed to N2 strain BC1270 unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Picked individual WT hermaphrodites. Screened for the absence of gravid Unc-22. Retained strain as BC1379. Balanced over eT1: BC1379 unc-22(s727)[BO]/nT1[N2] IV; sDf31[BO]/nT1[N2] hermaphrodite crossed to BC 1265 dpy-18(e364)/eT1 III; unc-46(e177)/eT1 V. Pick WT hermaphrodites that twitch in 1% nicotine. Retained one strain that segregated Unc-36 as BC1428. Pseudolinked sDf31 to dpy-18: BC1428 +/eT1 III; sDf31[BO]/eT1 V crossed to BC 1265 dpy-18/eT1 III; unc-46/eT1 V male. Picked WT hermaphrodite F1. From strains segregating Unc-36 but no Dpy or Unc-46 or DpyUnc-46, cross WT hermaphrodites to BC1265 males. From strains producing Dpy, crossed individual WT males to BC70 eT1;eT1. From each cross, crossed WT X WT. Kept one strain producing on Dpy or DpyUncs as S-H716 male. Picked one hermaphrodite to start BC1519. Comments: 1) The LGV region balanced by eT1 should be all BO. 2) Probably the rest of the genome still has a considerable amount from BO since it was crossed to N2 only 4 times. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1521 dpy-18(e364) III/eT1 [let-?(s1799)] (III;V); let-327(s247) unc-46(e177) V/eT1 [let-?(s1799)] (III;V). C. elegans Heterozygotes are WT and throw WT, DpyUncLet and dead eggs. DpyUncLet are translucent, have slow development, are cold sensitive and die as late larvals. Maintain by picking WT.
BC1549 dpy-18(e364)/eT1 III; unc-46(e177) let-349(s217)/eT1 V. C. elegans WT strain which segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal early larval. Maintain by picking WT.
BC1554 dpy-18(e364)/eT1 III; sDf32 unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1572 +/eT1 III; let-401(s193) dpy-11(e224) unc-42(e270)/eT1 V. C. elegans WT strain which segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal mid-larval. Maintain by picking WT.
BC1573 +/eT1 III; let-402(s127) dpy-11(e224) unc-42(e270)/eT1 V. C. elegans WT strain which segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal mid larval. Maintain by picking WT.
BC1574 +/eT1 III; let-403(s120) dpy-11(e224) unc-42(e270)/eT1 V. C. elegans WT strain which segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal mid-late larval. Maintain by picking WT.
BC1751 dpy-18(e364)/eT1 III; let-466(s1063) unc-46(e177)/eT1 V. C. elegans WT strain which segregates WT, Unc-36, dead eggs and DpyUncs. The DpyUncs throw DpyUncs that are Sterile adults. (Mel->Sterile adult in F2.) Maintain by picking WT.
BC1772 dpy-18(e364)/eT1 III; unc-46(e177) let-334(s908)/eT1 V. C. elegans Heterozygotes are WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT.
BC1781 dpy-18(e364)/eT1 III; sDf33 unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1782 dpy-18(e364)/eT1 III; sDf34 unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1784 dpy-18(e364)/eT1 III; unc-46(e177) sDf36/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1785 dpy-18(e364)/eT1 III; unc-46(e177) sDf30/eT1 V. C. elegans WT strain that segregates WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1919 dpy-18(e364)/eT1 III; unc-46(e177) let-343(s1025)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Egg lethal. Maintain by picking WT.
BC1922 dpy-18(e364)/eT1 III; let-342(s1029) unc-46(e177)/eT1 V. C. elegans WT strain that segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal mid-larval. Maintain by picking WT.
BC1924 dpy-18(e364)/eT1 III; unc-46(e177) let-340(s1022)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT.
BC1925 dpy-18(e364)/eT1 III; sos-1(s1031) unc-46(e177)/eT1 V. C. elegans WT strain that segregates WT, Unc-36 (eT1 homozygotes), and dead eggs. s1031 is an egg lethal. Maintain by picking WT. Previously called let-341(s1031).
BC1927 dpy-18(e364)/eT1 III; let-347(s1035) unc-46(e177)/eT1 V. C. elegans WT strain that segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal late larval. Maintain by picking WT.
BC1958 dpy-18(e364)/eT1 III; unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncs and dead eggs. Maintain by picking WT.
BC1999 dpy-18(e364)/eT1 III; nDf18/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT.
BC2179 dpy-18(e364)/eT1 III; mDf3/eT1 V. C. elegans Heterozygotes are WT and segregate WT, dead eggs and Unc-36. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. [2/99: Strain may have broken down: doesn't segregate real WT, and those animals that are not Unc-36 still are somewhat Unc; and unc-23 is no longer under mDf3 in this strain. Mike Nonet]
BC2200 dpy-18(e364)/eT1 III; unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, dead eggs and DpyUncs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC2237 dpy-18(e364)/eT1 III; let-348(s1436) unc-46(e177)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT.
BC2245 dpy-18(e364)/eT1 III; unc-46(e177) let-339(s1444)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, dead eggs and DpyUncLets. Lethal early larval. Maintain by picking WT.
BC2282 dpy-18(e364)/eT1 III; unc-46(e177) pst-1(s1481)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncs which arrest as embryos or as early larva and dead eggs.
BC2303 dpy-18(e364)/eT1 III; unc-46(e177) unc-70(s1502)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Uncs and DpyUnc Lets that hatch.
BC2383 dpy-18(e364)/eT1 III; unc-46(e177) let-469(s1582)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal as sterile adult. Maintain by picking WT. [7/94: No longer throwing eT1 Unc-36's. May have picked up a lethal mutation on one of the eT1 chromosomes. Also, appears to be lethal in mid-late larval stage rather than as adult.]
BC2409 dpy-18(e364)/eT1 [dpy-?] III; let-331(s1608) unc-46(e177)/eT1 [dpy-?] V. C. elegans Heterozygotes are WT and segregate WT, Unc-36???, Sterile DpyUncs, and dead eggs. Maintain by picking WT.
BC2420 dpy-18(e364)/eT1 III; unc-46(e177) let-346(s1619)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, dead eggs and DpuUncLets. Lethal mid-larval. Maintain by picking WT.
BC2422 dpy-18(e364)/eT1 III; unc-46(e177) let-476(s1621)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adults) and dead eggs. Maintain by picking WT.
BC2440 dpy-18(e364)/eT1 III; unc-46(e177) unc-70(s1639)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Uncs, and DpyUnc Lets that hatch.
BC2501 dpy-18(e364)/eT1 III; unc-46(e177) sDf57/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC2504 dpy-18(e364)/eT1 III; unc-46(e177) let-406(s514)/eT1 V. C. elegans WT strain that segregates WT, Unc-36, dead eggs and mid larval DpyUnc lethals. Maintain by picking WT.
BC2507 dpy-18(e364)/eT1 III; unc-60(e677) cdc-25.2(s819) dpy-11(e224)/eT1 V. C. elegans WT strain which segregates WT, Unc-36, and dead eggs. Egg lethal. Maintain by picking WT.
BC2509 dpy-18(e364)/eT1 III; unc-60(e677) dpy-11(e224) let-408(s827)/eT1 V. C. elegans WT strain that segregates WT, Unc-36 and dead eggs. Egg lethal. Maintain by picking WT.