CZ18412 |
juSi94 II; rps-18(ok3353) IV; glo-4(ok623) V; juEx5515. |
juSi94 [GFP11::rps-18 + Cbr-unc-119(+)] II. juEx5515 [unc-25p::GFP1-10 + unc-25p::mCherry::rab-3 + ttx-3p::RFP]. Pick ttx-3::RFP to maintain. GABAergic motor neuron-specific expression of split GFP reporter allows visualization of ribosomes in neurons, and GABAergic motor neuron-specific expression of mCherry::rab-3. Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. |
FK134 |
ttx-3(ks5) X. |
Abnormal in thermotaxis. Cryophilic (cold-seeking) and abnormal in isothermal tracking. |
GR1395 |
mgIs49 IV. |
mgIs49 [mlt-10p::GFP::PEST + ttx-3::GFP] IV. GFP expression oscillates with molting cycle. Reference: Veli SM, et al. Mol Biol Cell. 2010 May 15;21(10):1648-61. PMID: 20335506. |
OH1078 |
ttx-3(ot22) X; otEx621. |
otEx621 [(pAIY-MCS) ttx-3(cDNA) + unc-47(long)::GFP]. Maintain by picking GFP+. |
OH1098 |
otIs133 II. |
otIs133[pttx-3::RFP + unc-4(+)]. RFP expressed in AIY only. |
OH160 |
otIs76 mgIs18 IV; lon-2(e678) hst-6(ot17) X. |
otIs76 [pttx-3p::kal-1 + unc-122p::GFP] IV. mgIs18 [ttx-3p::GFP] IV. Long, otherwise phenotypically WT. ttx-3p::GFP labels AIY interneurons. hst-6(ot17) suppresses the branching phenotype of KAL-1 overexpression in AIY. hst-6(ot17) is closely linked to lon-2. |
OH161 |
ttx-3(ot22) X. |
Thermotaxis defective (cryophilic). Null allele. |
OH2018 |
mgIs18 IV; otIs35 hst-6(ot19) X. |
mgIs18 [ttx-3p::GFP] IV. otIs35 [ttx-3p::kal-1 + rol-6(su1006)] X. otIs35 has been mapped to LG X between lon-2 and hst-6. Rollers. ttx-3p::GFP labels AIY interneurons. ot19 suppresses the branching phenotype of KAL-1 overexpression in AIY. hst-6(ot19) is closely linked to otIs35. |
OH4343 |
otIs133 II; otEx2498. |
otEx2498 [gcy-18(prom1)::GFP + rol-6(su1006)]. Expresses GFP in AFD and AIM. Maintain by picking GFP+ animals (which should also be Rollers). otIs133 [pttx-3::RFP + unc-4(+)]. RFP expressed in AIY only. |
OH4768 |
kyIs140 I; ttx-3(mg158) X. |
kyIs140 [str-2::GFP + lin-15(+)] I. |
OH4769 |
ttx-3(mg158) X; nuIs11. |
nuIs11 [osm-10::GFP + lin-15(+)]. Array contains pool28; KP#57-59 (osm-10::GFP insert at Nrul site) and pJM24 [lin-15(+) rescues n765 at 20C]. GFP expressed in ASH, ASI, PHA and PHB after 3-fold. nuIs11 may be inserted on LG I. |
OH4770 |
ttx-3(mg158) X; otIs24. |
otIs24 [sre-1::GFP + dpy-20(+)]. |
OH7074 |
otIs173 III; ttx-3(ot355) X. |
otIs173 [F25B3.3::DsRed2 + ttx-3promB::GFP] III. Whole genome sequenced strain. Reference: Sarin S, et al., Genetics. 2010 Jun;185(2):417-30. |
OH7075 |
otIs173 III; ttx-3(ot356) X. |
otIs173 [F25B3.3::DsRed2 + ttx-3promB::GFP] III. Whole genome sequenced strain. Reference: Sarin S, et al., Genetics. 2010 Jun;185(2):417-30. |
OH7076 |
otIs173 III; ttx-3(ot357) X. |
otIs173 [F25B3.3::DsRed2 + ttx-3promB::GFP] III. Whole genome sequenced strain. Reference: Sarin S, et al., Genetics. 2010 Jun;185(2):417-30. |
OH7079 |
otIs173 III; ttx-3(ot360) X. |
otIs173 [F25B3.3::DsRed2 + ttx-3promB::GFP] III. Whole genome sequenced strain. Reference: Sarin S, et al., Genetics. 2010 Jun;185(2):417-30. |
OH7103 |
otIs173 III; ttx-3(ot365) X. |
otIs173 [F25B3.3::DsRed2 + ttx-3promB::GFP] III. Whole genome sequenced strain. Reference: Sarin S, et al., Genetics. 2010 Jun;185(2):417-30. |
OH8 |
ttx-3(mg158) X. |
Thermotaxis defective (cryophilic); strong loss of function. |
OH910 |
otIs77 II. |
otIs77 [ttx-3p::kal-1 + unc-122p::GFP] II. Overexpressing line from P. Loria. The integrated array has been mapped to LG II between stP36 and maP1. AIY interneurons have a 100% penetrant axon branching phenotype. Otherwise, the animals look phenotypically wild type. |
OH912 |
otIs76 mgIs18 IV. |
otIs76 [pttx-3p::kal-1 + unc-122p::GFP] IV. mgIs18 [ttx-3p::GFP] IV. otIs76 is closely linked to mgIs18 on LG IV. Superficially wild-type. AIY interneurons have a 100% penetrant axon branching phenotype. |
OH9331 |
ttx-3(ot358). |
Whole genome sequenced strain. |
OH99 |
mgIs18 IV. |
mgIs18 [ttx-3p::GFP] IV. ttx-3p::GFP labels AIY interneurons. |