Gene Information: fem-3

Namefem-3 View on WormBase
Species C. elegans
SequenceC01F6.4
Genetic positionIV:4.05 +/- 0.002 cM
Genomic positionIV: 9104966..9107607

Strains carrying this gene

Strain Genotype Description
CB3843 fem-3(e1996)/unc-24(e138) dpy-20(e1282) IV. Heterozygotes are WT and segregate WT, DpyUnc and fertile females.
CB3844 fem-3(e2006) IV. Temperature sensitive Fem. Hermaphrodites at 15C, female at 25C. Maintain at 15C.
CB4015 fem-3(e1999) IV. Weakest known fem-3 mutation. Viable non-temperature-sensitive self-fertile XX hermaphrodites. e1999 XO males are slightly feminized, sometimes male-fertile. Reference: Hodgkin (1986) PMID: 3770465.
CB4760 fem-1(e2044) mor-2(e1125) unc-24(e158) fem-3(q20) / unc-5(e53) dpy-20(e1282) IV Wild-type hermaphrodites segregating wild-type hermaphrodites, Unc-24 females, Unc Dpy hermaphrodites. Maintain by picking wild-type hermaphrodites. Deletion allele of fem-1, with unusual maternal effect. Reference: Spence AM, et al. Cell. 1990 Mar 23;60(6):981-90. Johnson CL & Spence AM.. Science. 2011 Sep 2;333(6047):1311-4.
CP87 Cbr-fem-3(nm63) IV. XO are self-fertile hermaphrodites with low brood size and some somatic gonad defects. XX animals have normal brood size.
CP89 Cbr-fem-2(nm27) III; Cbr-fem-3(nm63) IV. XX are self-fertile hermaphrodites.
JK1973 fem-3(q96) IV. Semi-dominant Mog. Temperature-sensitive sterile. Maintain at 15C. Masculization of germline (Mog) at 25C.
JK3965 fbf-1(ok91) fbf-2(q704)/mIn1 [mIs14 dpy-10(e128)] II; fem-3(e1996)/qIs24 IV. qIs24 [lag-2p::GFP]. Maintain by picking individual animals with green DTCs and green pharynx and scoring offspring for Fems and Fbfs. fem-3 is not well balanced by qIs24. fbf-1 fbf-2 homozygotes are germline proliferation defective and make only sperm. fem-3 homozygotes make only oocytes. fbf-1 fbf-3; fem-3 proliferates more than fbf-1 fbf-2 and makes only oocytes; also Muv. qIs24 contains lag-2::GFP; Distal Tip Cells are green; homozygotes are Rollers and heterozygotes Roll weakly. mIn1 is Dpy and GFP+ in the pharynx. Do not distribute this strain; other labs should request it from the CGC.
JK551 unc-5(e53) fem-3(q22) IV. Temperature sensitive. At 25C, XX germline makes only sperm; at 15C, germline makes oocytes and excess sperm. Unc. Do not distribute this strain; other labs should request it from the CGC.
JK654 fem-3(q23) IV. Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC.
JK816 fem-3(q20) IV. Gain of function. Temperature sensitive. XX germline makes only sperm at 25C; XX germline makes oocytes and excess sperm at 15C. Do not distribute this strain; other labs should request it from the CGC.
JK860 fem-3(q20) IV; fog-2(q71) V. Temperature-sensitive. Maintain at 15 C. Do not distribute this strain; other labs should request it from the CGC.