| PS8829 |
col-156(sy1536) V. |
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of col-156;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: GTCGAACACGTTCATCGCTGGACTCACCACTGT
Right flanking sequence: ATCCGGTGTAGCGATTCTCGGATGTCTTTTGTTTTTG
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : GCTGGACTCACCACTGTATC
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616 |