||Maternally rescued. Homozygous e2519 worms coming from homozygous mothers develop slowly. Adult rhythmic behaviors are also slowed.
||clk-1(e2519) III; rte-5(qm197) X.
||qm197 suppresses the L2 arrest and sterility of clk-1(e2519) on UQ- bacteria.
||Maternally rescued slow growing strain. Grows better at 15C. See also WBPaper00002456.
||clk-1(e2519) III; rte-1(qm199) X.
||qm199 suppresses the growth arrest and sterility of clik-1(e2519) on ubi- bacteria.
||rte-2(qm210) I; clk-1(e2519) III.
||qm210 suppresses the growth arrest and sterility of clik-1(e2519) on UQ- bacteria.
||clk-1(e2519) III; rte-4(qm213) X.
||qm213 suppresses the growth arrest and sterility of clik-1(e2519) on ubi- bacteria.
||clk-1(e2519) III; rte-3(qm211) X.
||qm211 suppresses the growth arrest and sterility of clik-1(e2519) on ubi- bacteria.
||dpy-17(e164)/clk-1(e2519) clk-2(qm37) III.
||Hets are WT and segregate WT, Dpys and maternally rescued clk double mutants (WT). To study clk double mutants, pick WT worms singly onto plates. Maternally rescued clk-1 clk-2 worms segregate very slow hatching and developing progeny and no Dpys. Double mutants do not give a viable strain but rather die out over a few generations.
||clk-3(qm38) II; clk-1(qm30)/dpy-17(e164) III.
||Develops slowly (clk-3(qm38) rate). clk-1(qm30) is maternally rescued. Segregates 1/4 Dpy progeny. To study clk-3; clk-1 doubles place WT worms singly onto plates-the clk doubles will throw no Dpys and their progeny develop extremely slowly (about 3 days to hatch and 10 days for postembryonic development)-they will be sterile when they finally become adults.
||daf-2(e1370) clk-1(e2519) III.
||Daf-c at 25C. Slower growing than clk-1(e2519).
||clk-1(ok1247) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
||ZC395.2 Heterozygotes are WT and GFP+. Maintain by picking GFP+ worms. ok1247 animals are homozygous viable. qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. Outer Left Sequence: CGGGTTTCGCACTATTTTGT. Outer Right Sequence: CAGCTACCGTACCCGACATT. Inner Left Sequence: GCTGGCCCAGTACATTTGTT. Inner Right Sequence: CAGTGTTCCGGATTTCAGGT. Inner Primer PCR Length: . Estimated Deletion Size: about 1250 bp. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807