| PS8031 |
cest-1.1(sy1180) V. |
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of cest-1;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: CAATTGGAGACCTTCGATATCGAAAACCCCGTCCACCG
Right flanking sequence: AAATCATGGGAAGGAGTTTTGGTAACAAATGAATA
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : ACTCCTTCCCATGATTTCGG
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616 |