| PS8120 |
gnrr-4(sy1196) X. |
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of gnrr-4;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: CGGACTGCATCGTTCTCTTTATCTACGCTCCAACT
Right flanking sequence: CAGTTTGCATGGATTCACTCATACTGGgtaagtctg
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : TGAATCCATGCAAACTGAGT
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616 |