| PS8990 |
otpl-8(sy1592) X. |
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of otpl-8;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: CATCACCCACACATCATCATGAACTCTACCGACGA
Right flanking sequence: CCTTGGATTCATGAGCCAAGAGCCACgtaagtctag
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : ATGAACTCTACCGACGACCT
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616 |