| PS8936 |
otpl-2(sy1579) V. |
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of otpl-2;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: CATGAAAATATGAAACGTTGGACGAAAAACCCGGAA
Right flanking sequence: GCAAGgtaaaaagggaataactcaatataattc
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : GGACGAAAAACCCGGAAGCA
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616 |