| PS8303 |
oac-59(sy1260) IV. |
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of oac-59;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: CCACCGTCTTCAAAACGGCTGGACCTTCAAGGCAT.
Right flanking sequence: TAGAGGGTTGGCAATTCTATCAGTTCTGGGATTTC
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : CTGGACCTTCAAGGCATTAG
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616 |