Gene Information: puf-3
| Name | puf-3 View on WormBase |
|---|---|
| Species | C. elegans |
| Sequence | Y45F10A.2 |
| Genetic position | IV:9.43 +/- 0.017 cM |
| Genomic position | IV: 13510307..13512162 |
Strains carrying this gene
| Strain | Genotype | Description |
|---|---|---|
| JK5915 | puf-3(q966) IV. | Superficially wild-type. |
| JK6080 | puf-3(q1058[3xV5::puf-3]) IV. | 3x V5 epitope tags inserted into endogenous puf-3 locus. Expresses functional 3x V5-tagged PUF-3. Reference: Haupt KA, Genetics. 2020 Jan;214(1):147-161. PMID: 31740451 |
| JK6321 | puf-3(q966) puf-11(q971) IV/ nT1[qIs51] (IV;V). | Homozygous maternal effect lethal double mutant balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested GFP+ nT1[qIs51] aneuploids, and non-GFP puf-3(q966) puf-11(q971) homozygotes (maternal effect lethal). Homozygous nT1[qIs51] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. |