| NQ792 |
qnIs303 IV; dmsr-1(qn45) V. |
qnIs303 [hsp-16.2p::flp-13 + hsp-16.2p::GFP + rab-3p::mCherry] IV. Pumps and moves 2 hours after heat induced flp-13 over-expression. Outcrossed 1x to NQ570. Reference: Iannacone M, et al. eLife 2017. |
| NQ915 |
dmsr-1(qn45) V. |
Pumps and moves 30 minutes after 30-minute 35 deg heat shock. Derived by outcrossing NQ792 to remove transgene. Reference: Iannacone M, et al. eLife 2017. |
| PS8789 |
dmsr-1(sy1522) V. |
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-1.
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
left flanking sequence: CCTATACATGCCTACTTATCAATATTTCTATGCGT
right flanking sequence: GTTGGGTACAATCGCTAATTTCTGTAACATCGTCG
inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TCAATATTTCTATGCGTGTT
Method Reference: G3 (Bethesda). |