| BOX163 |
erm-1(mib9[erm-1[T544D]]) I. |
Homozygous viable. Modification of endogenous erm-1 locus mimics ERM-1(T544) phosphorylation. Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 |
| BOX165 |
erm-1(mib10[erm-1[T544A]]) I. |
Homozygous viable. Modification of endogenous erm-1 locus mimics non-phosphorylated ERM-1(T544). Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 |
| BOX213 |
erm-1(mib15[erm-1::eGFP]) I. |
Endogenous erm-1 locus tagged with eGFP. Homozygous viable, partially functional endogenous erm-1 tag. erm-1::GFP animals have a reduced brood size and incomplete outgrowth of the excretory canal, but show no other developmental or morphological abnormalities. |
| BOX215 |
erm-1(mib16[erm-1[T544D]::GFP]) I. |
Homozygous viable. Endogenous erm-1 locus tagged with eGFP and modified to mimic ERM-1(T544) phosphorylation. Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. eGFP-tagged ERM-1 is not fully functional: animals have a reduced brood size and incomplete outgrowth of the excretory canal, but show no other developmental or morphological abnormalities. The penetrance of intestinal phenotypes is slightly higher than in untagged T544 mutants, presumably owing to a detrimental influence of the COOH-terminal GFP tag. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 |
| BOX218 |
erm-1(mib19[erm-1[T544A]::GFP]) I. |
Homozygous viable. Endogenous erm-1 locus tagged with eGFP and modified to mimic non-phosphorylated ERM-1(T544). Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. eGFP-tagged ERM-1 is not fully functional: animals have a reduced brood size and incomplete outgrowth of the excretory canal, but show no other developmental or morphological abnormalities. The penetrance of intestinal phenotypes is slightly higher than in untagged T544 mutants, presumably owing to a detrimental influence of the COOH-terminal GFP tag. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 |
| VC2616 |
erm-1(ok3268)/szT1 [lon-2(e678)] I; +/szT1 X. |
C01G8.5. Homozygous viable deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3268 homozygotes. Pick WT and check for correct segregation of progeny to maintain. External left primer: TGTTGAGTGTGTTGTTGCGA. External right primer: GCGCACATCCTTTTTCATTT. Internal left primer: ACAATCAGGGATTCCGTTTT. Internal right primer: TGGATGGAACATTTTGTGGA. Internal WT amplicon: 1263 bp. Deletion size: 528 bp. Deletion left flank: TTGTCTTGTAACAGTAGTCTGTCTCTCGAC. Deletion right flank: GTTTGTTGATACGGAGTCGTGGAGCGTAGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
| VC2617 |
erm-1(ok3269)/szT1 [lon-2(e678)] I; +/szT1 X. |
C01G8.5. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3269 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TGTTGAGTGTGTTGTTGCGA. External right primer: GCGCACATCCTTTTTCATTT. Internal left primer: ACAATCAGGGATTCCGTTTT. Internal right primer: TGGATGGAACATTTTGTGGA. Internal WT amplicon: 1263 bp. Deletion size: 1068 bp. Deletion left flank: GAAAACATTTAAAAAAATGTTTATCAAAAA. Deletion right flank: CATTTTTTCGATTTTTTTTTCAGCGAAAAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 |
| VJ311 |
erm-1(tm677)/unc-63(x18) dpy-5(e61) I. |
Heterozygotes are WT and segregate WT, DpyUncs, and erm-1 homozygotes which grow up to bagging adults with few progeny. Some of these hatch but die as L1s. |
| VJ317 |
erm-1(tm677) I; sDp2 (I;f). |
Animals which have lost the Dp grow up to bagging adults with few progeny. Some of these hatch but die as L1s. |