| SP483 |
lem-3(mn155) I. |
Extremely hypersensitive to UV, X and gamma irradiation. |
| TG4267 |
lem-3(gt3309[eGFP::Stag::lem-3]) I. |
GFP tag inserted into endogenous lem-3 locus by CRISPR. Reference: Hong Y, et al. Nat Commun. 2018 Feb 20;9(1):728. |
| TG4298 |
lem-3(gt3310[eGFP::STag::lem-3[S192A S194A]]) I. |
Endogenous lem-3 locus carries GFP tag and two misense mutations in putative phosphorylation sites [S192 S194]. Homozygous viable, though [S192A S194A] mutants exhibit increased embryonic lethality after irradiation. Reference: Hong Y, et al. Nat Commun. 2018 Feb 20;9(1):728. |
| TG4300 |
lem-3(gt3311[eGFP::Stag::lem-3[Y556A G558A]]) I. |
Endogenous lem-3 locus carries GFP tag and two misense mutations in conserved residues [Y556A G558A] of GIY-YIG nuclease domain. Homozygous viable, though [Y556A G558A] mutants exhibit increased embryonic lethality after irradiation and abolished localization of GFP::LEM-3 at the midbodies. Reference: Hong Y, et al. Nat Commun. 2018 Feb 20;9(1):728. |
| TG4319 |
lem-3(tm3468) I. |
Homozygous viable. Increased embryonic lethality after irradiation. |