| NL713 |
mut-2(r459) I; sox-2(pk65). |
K08A8.2. Location of the insertion: TCACGTATCT TA CATATTATAT. |
| OH10051 |
unc-119(ed3) III; sox-2(ot640[unc-119(+)])/+ X. |
ot640 was generated by using MosDel removing the entire sox-2 locus and insert unc-119(+). Heterozygotes are WT and segregate WT heterozygotes, Uncs and ot640 homozygous that arrest in L1 with a pharynx unattached (Pun) phenotype. Maintain by picking WT and check for correct segregation of progeny to maintain. Reference: Vidal B, et al. Development. 2015 Jul 15;142(14):2464-77. |
| OH10053 |
unc-119(ed3) III; sox-2(ot640[unc-119(+)]) X; otEx4454. |
otEx4454 [sox-2(fosmid)::mCherry + elt-2p::DsRed]. ot640 was generated by using MosDel removing the entire sox-2 locus and insert unc-119(+). ot640 homozygous arrest in L1 with a pharynx unattached (Pun) phenotype. Maintain by picking WT to maintain (transgene should be required for viability). Reference: Vidal B, et al. Development. 2015 Jul 15;142(14):2464-77. |
| RW12244 |
sox-2(st12244[sox-2::TY1::EGFP::3xFLAG]) X. |
CRISPR/Cas9 engineered tagged endogenous locus. |